Interspecific Incompatibility Amongst Victorian Species of Amyema (Loranthaceae)

1982 ◽  
Vol 30 (2) ◽  
pp. 175 ◽  
Author(s):  
P Bernhardt

Each of the six Amyema species collected in Victoria had a natural distribution and floral phenoiogy that overlapped with at least one of its Victorian congenerics. All of the Amyema spp. were pollinated by the same family of birds (Meliphagidae, honeyeaters) and often by the same species. All intraspecific cross-pollinations in vitro consistently produced an average of more than five pollen tubes per style which grew more than 0.125 the length of the style within 24 hours. Seventeen of 30 interspecific pollinations in vitro failed to produce pollen tubes in the style over the same time. Only eight of the 30 interspecific crosses attempted produced results competitive with respective intraspecific crosses. The success of the interspecific cross appeared to depend on the breeding system of the species receiving pollen from another. Self-incompatible Amyema pendulum (Sieb. ex Spreng.) Van Tieghem and Amyema miraculosum (Miq.) Van Tieghem repeatedly failed to accept the pollen of all the other species. Seif- compatible Amyema preisii (Miq.) Van Tieghem and Amyema quandang (Lindl.) Van Tieghem showed good to excellent interspecific pollen tube penetration of stylar tissue. Partially self-compatible Amyema linophyllum (Fenzl) Van Tieghem and Amyema miquelii (Lehm. ex Miq.) Van Tieghem showed results intermediate between self-compatible and self-incompatible taxa. Geographic and floral phenological barriers contribute partially to interspecific isolation as intercompatible species may have regionally different flowering seasons and distributions. F, hybrids recorded in southern Australia show extremely low rates of pollen viability and seed production. There is no current evidence that introgression occurs.

Author(s):  
Maha Mezied ◽  
Norah Alnasser ◽  
Reema Al Owaid ◽  
Rand Bakhsh ◽  
Lama Alkhudhayr ◽  
...  

The accuracy of the intraoral digital impression systems has been previously reported to refer to the quality of the obtained data from the related scanning procedures, irrespective of the quality of the clinical outcomes and the estimated costs. Trueness and precision have been frequently found among studies in the literature as two terms describing the accuracy of the intraoral digital impression systems. Various digital impression modalities have been proposed among studies in the literature and were investigated for estimation of their accuracy. The paper aims to review and discuss the most common literature regarding the current common systems and their accuracy among the different studies in the literature. According to the results, Chairside Economical Restoration of Esthetic Ceramic (CEREC) systems appear to have the highest estimated rates of trueness and precision as compared to the other modalities while the iTero system appears to have the lowest estimated rates. However, some studies have reported contradicting results and the current evidence is mainly based on findings from in vitro investigations. Accordingly, further studies might be needed for further validation of the current evidence and strengthening the quality of the future potential implications for clinicians. 


2000 ◽  
Vol 125 (2) ◽  
pp. 265-270 ◽  
Author(s):  
A.M.S. Nyomora ◽  
P.H. Brown ◽  
K. Pinney ◽  
V.S. Polito

The effect of boron (B) on in vivo and in vitro development of almond [Prunus dulcis (Mill.) D.A. Webb (syn. P. amygdalus Batsch)] pollen and pollen tubes and the resultant effect on fruit set was studied in mature trees. The cultivars Mono (pistil donor) and Butte (pollinizer) in an orchard with low soil B in Fresno, California were sprayed with B at 0, 0.8, 1.7, or 2.5 kg·ha-1 during Fall 1993. Pollen viability as indicated by the fluorescein diacetate method (FDA) was >85% and was not affected by field-applied B, however, in vivo pollen germination and tube growth were enhanced by foliar-applied B. More effect of applied B on in vivo growth appeared as pollen tubes progressed toward the ovary. For in vitro germination, foliar-applied B reduced bursting of tubes, and addition of B to the culture media significantly increased pollen germination and pollen tube growth.


HortScience ◽  
1992 ◽  
Vol 27 (5) ◽  
pp. 425-427 ◽  
Author(s):  
Gregory A. Lang ◽  
E. James Parrie

Pollen from six southern highbush blueberry cultivars derived from Vaccinium corymbosum L. and one or more other species (V. darrowi Camp, V. ashei Reade, and V. angustifolium Aiton) was incubated on nutrient agar to determine tetrad viability, pollen tube growth rates, and incidence of multiple pollen tube germinations. `Avonblue' pollen had a significantly lower tetrad germination percentage than `Georgiagem', `Flordablue', `Sharpblue', `Gulfcoast', or `O'Neal', all of which had >90% viable tetrads. The in vitro growth rate of `O'Neal' pollen tubes was significantly higher than the growth rates of `Sharpblue' and `Georgiagem pollen tubes. Of those tetrads that were viable, more than two pollen tubes germinated from 83% and 91% of the `Gulfcoast' and `Sharpblue' tetrads, respectively, while only 11% of the `Flordablue' tetrads produced more than two pollen tubes. The total number of pollen tubes germinated per 100 tetrads ranged from 157 (`Flordablue') to 324 (`Sharpblue'), resulting in actual pollen grain viabilities ranging from 39% to 81%. Genetic differences in pollen vigor, as indicated by pollen viability, pollen tube growth rates, and multiple pollen tube germinations, may influence blueberry growers' success in optimizing the beneficial effects of cross-pollination on fruit development.


HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 585-586 ◽  
Author(s):  
Sierd Zijlstra ◽  
Coen Purimahua ◽  
Pim Lindhout

Crossing barriers between white- and purple-flowered species were examined. Four accessions of Capsicum annuum and three of C. pubescens were reciprocally crossed with one to four accessions of C. baccatum, C. cardenasii, C. chacoense, C. chinense, C. eximium, C. frutescens, C. galapagoense, and C. praetermissum. Capsicum chacoense is the only white-flowered species that inhibits C. annuum pollen tube growth but allows C. pubescens pollen tube penetration into the egg cell. Capsicum cardenasii and C. eximium exhibit similar crossabilities with C. annuum and C. pubescens: pollen tubes of C. cardenasii and of C. eximium can penetrate the egg cells of C. annuum but not vice versa, and pollen tubes of C. pubescens can penetrate the egg cells of C. cardenasii and of C. eximium but not vice versa.


2012 ◽  
Vol 59 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Wojciech Antkowiak ◽  
Andrzej Wojciechowski

Interspecific compatibility and self-compatibility in <i>Pyrus communis</i>, <i>P. pyraster</i> and <i>P. salicifolia</i> were evaluated. Degree of compatibility was determined by means of fluorescence microscope. Self-incompatibility evaluated on the basis of the pollen tubes growth showed that two cultivars of <i>P. communis</i> (Bera Hardy and Lukasówka) were self-compatible and the other three cultivars were self-incompatible. All ecotypes of <i>P. pyraster</i> are self-incompatible. In interspecific crosses full inter-incompatibility or unilateral self-incompatibility was observed.


2005 ◽  
Vol 130 (3) ◽  
pp. 341-347 ◽  
Author(s):  
Nadine Ledesma ◽  
Nobuo Sugiyama

The effects of high-temperature stress on pollen viability and in vitro and in vivo germinability were studied in two facultative, short-day strawberries (Fragaria ×ananassa Duch.), `Nyoho' and `Toyonoka.' Plants were exposed to two day/night temperature regimes of either 23 °C/18 °C (control) or 30 °C/25 °C (high temperature) from when the first inflorescence became visible until anthesis. Pollen viability in `Nyoho' was only slightly affected at 30 °C/25 °C when compared with pollen from plants grown at 23 °C/18 °C. In `Toyonoka', however, pollen viability was significantly lower at 30 °C/25 °C than at 23 °C/18 °C. The in vitro germination percentages were significantly lower in pollen from plants grown at 30 °C/25 °C and germinated at 30 °C than from plants grown at 23 °C/18 °C and germinated at 23 °C in both cultivars. But the percentages were much lower in `Toyonoka' than in `Nyoho', particularly at the 30 °C germination temperature. Pollen from plants grown at 23 °C/18 °C also extended longer pollen tubes than pollen grown at 30 °C/25 °C in both cultivars, but `Nyoho' had longer pollen tubes than `Toyonoka' at 30 °C/25 °C. Fluorescence microscopy revealed that most of the `Nyoho' pollen germinated on the stamen, elongated through the style and reached the ovule regardless of temperature treatment. In `Toyonoka', pollen germination and elongation were greatly inhibited at 30 °C/25 °C, resulting in unfertilized ovules. These results suggest that certain strawberry cultivars produce heat-tolerant pollen, which in turn could result in higher fruit set.


1970 ◽  
Vol 40 (1) ◽  
pp. 93-95 ◽  
Author(s):  
Feruzan Dane ◽  
Nuran Ekici

In vitro and in vivo studies on pollen germination of Paeonia tenuifolia L. (Paeoniaceae) revealed that pollen grains are shed at two-celled stage. Normal and abnormal pollens were observed. Pollen viability was recorded between 55 and 75%. In vitro studies revealed 85% germination and usually the germination was monosphonic. Some pollen tubes with swollen tube tip and undulations were found. Styles and stigma were found to contain many pollen tubes 24 hrs after pollination. Key words: Paeonia tenuifolia; Pollen tube; In vitro growth; In vivo growth  DOI: http://dx.doi.org/10.3329/bjb.v40i1.8003 Bangladesh J. Bot. 40(1): 93-95, 2011 (June)


2020 ◽  
Vol 23 (1) ◽  
pp. 52-58
Author(s):  
Y. Arnaoudova ◽  
B. Arnaoudov

Yield realizing in vegetable crops depends on several reproductive processes that include pollen fertility as well as pollen tubes germination and growth. The study was conducted as the pollen characteristics fertility and viability were used, as well as the productivity for evaluation of drought tolerance in four pepper genotypes Capsicum annuum L. From the conducted cytological analyses was found that decreasing the water standard with 50% exert negative influence over the male gametophyte and the pollen fertility decreases with up to 37.4% average. More sensitive to drought showed to be the plants from both cultivars Pazardzhishka kapia and Albena, in which lower productivity and lower restorable potential were found in comparison with those found in the mutant lines No.1936 and No.1917. Increased tolerance showed the male gametophyte of line No.1936. After a drought in it were found the highest values for pollen viability – 33.4% average pollen germination and 438.7µm pollen tubes length which was with proven mathematical difference compared with the rest of the genotypes. These results showed that pollen fertility analyses and pollen viability analyses in drought plants could be used for evaluation of the drought tolerance.


HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 683e-683
Author(s):  
José G. Levy ◽  
Dina A. St. Clair

High temperatures during flowering have been implicated in reducing seed set and fruit set in tomatoes (Lycopersicon esculentum). Pollen viability and vigor were studied by measuring in vitro germination and pollen tube development in pollinated pistils of four processing tomato cultivars under normal (25° C day/15° C night) and high (32° C day/23° C night) temperatures. Preliminary studies were carried out to determine the length of pollen tubes in styles collected in times ranging from 3 to 48 hours after pollination. Under normal temperatures the pollen tubes reach the end of the style between 12 and 18 hours. At high temperatures there are fewer pollen tubes moving through the style and the time to reach the end of the style is longer. In pollen vigor studies, crosses were made between pollen and pistils of plants grown under different temperature treatments, then pollinated pistils were collected at 4, 8 and 12 hours after pollination. There were differences in in vitro pollen germination percentage and pollen tube length in the pollinated pistils, suggesting that high temperatures act to slow down pollen activity.


1990 ◽  
Vol 68 (5) ◽  
pp. 1163-1165 ◽  
Author(s):  
D. Eisikowitch ◽  
M. A. Lachance ◽  
P. G. Kevan ◽  
S. Willis ◽  
D. L. Collins-Thompson

The yeast Metschnikowia reukaufii is a natural contaminant of nectar and is vectored to the flowers of the field milkweed Asclepias syriaca by insects, some of which are pollinators of the plants. In its natural habitat, the yeast inhibits the germination of the milkweed's pollen, which normally uses nectar in the stigmatic cavity for germination. This inhibition is irreversible after about 8 h of exposure to the yeasts. Two selected strains of the yeast were isolated and investigated for their effects on pollen germination in vitro. The two strains, and their mixture, affected pollen germination adversely by reducing its amount and vigour and causing any pollen tubes that were produced to burst: One strain was more virulent than the other, and the mixture seemed to have an additive effect. The strains may be more efficacious than the natural assemblage of microbes in disrupting fertilization of milkweed flowers because they cause the immediate death (bursting) of the growing microgametophyte (pollen and tube). Key words: yeasts, pollen germination, milkweed, Metschnikowia reukaufii, Asclepias syriaca.


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