Exine Ultrastructure of Myrtaceous Pollen

1982 ◽  
Vol 30 (1) ◽  
pp. 75 ◽  
Author(s):  
PA Gadek ◽  
HA Martin

Exine ultrastructure has been determined by transmission electron microscopy analysis of the grains of a number of species of Myrtaceae, chosen to represent some of the diversity of sculpture and structure that can be observed using light microscopy and scanning electron microscopy. The species all show a typical angiosperm exine differentiation consisting of two chemically different layers, an electron-dense ektexine and a less dense endexine divided by a very thin electron-transparent lamella. They all differ, however, from the typical angiosperm architecture by the presence of a somewhat unstructured, granulate infratectal layer and a granular/alveolate endexinous layer around the pores. Species differences relate to the granulate coiumellate organization of the infractectal layer, the extent or density of tectal perforations and the presence and thickness of a foot layer around the grain.

2018 ◽  
Vol 44 (4) ◽  
pp. 287-291
Author(s):  
Rafael Manfro ◽  
Gislaine Felipe Garcia ◽  
Marcelo Carlos Bortoluzzi ◽  
Vinicius Fabris ◽  
Atais Bacchi ◽  
...  

Author(s):  
Hongyan Xu ◽  
Jing Guo ◽  
Qing Meng ◽  
Zhanling Xie

<i>Morchella</i> is a genus of edible fungi with strong resistance to Cd and the ability to accumulate it in the mycelium. However, the mechanisms conferring Cd resistance in <i>Morchella</i> are unknown. In the present study, morphological and physiological responses to Cd were evaluated in the mycelia of <i>Morchella spongiola</i>. Variations in hyphal micro-morphology including twisting, folding and kinking in mycelia exposed to different Cd concentrations (0.15, 0.9, 1.5, 2.4, 5.0 mg/L) were observed using scanning electron microscopy. Deposition of Cd precipitates on cell surfaces (at Cd concentrations > 2.4 mg/L) was shown by SEM-EDS. Transmission electron microscopy analysis of cells exposed to different concentrations of Cd revealed the loss of intracellular structures and the localization of Cd depositions inside/outside the cell. FTIR analysis showed that functional groups such as C=O, -OH, -NH and -CH could be responsible for Cd binding on the cell surface of <i>M. spongiola</i>. In addition, intracellular accumulation was observed in cultures at low Cd concentrations (< 0.9 mg/L), while extracellular adsorption occurred at higher concentrations. These results provide valuable information on the Cd tolerance mechanism in <i>M. spongiola</i> and constitute a robust foundation for further studies on fungal bioremediation strategies.


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