Shoot Regeneration in vitro From Native Australian Fruit-Bearing Trees - Quandong and Plum Bush

1980 ◽  
Vol 28 (4) ◽  
pp. 405 ◽  
Author(s):  
M Barlass ◽  
WJR Grant ◽  
KGM Skene
Keyword(s):  
Shoot Growth ◽  
Adventitious Shoot ◽  
Indolebutyric Acid ◽  
Axillary Shoots ◽  
Aseptic Culture ◽  
Aerial Parts ◽  
Edible Fruit ◽  
Semiarid Areas ◽  
Regeneration In Vitro

Explants from quandong (Santalum acuminatum) and plum bush ( S. lanceolatum), native Australian trees, regenerated shoots in aseptic culture. In the presence of cytokinin. shoots were produced in quantity from all cultured aerial parts of quandong seedlings. Individually cultured nodes of I-yr-old plum bush and quandong plants proliferated axillary shoots, while internodal pieces produced adventitious shoot growth. Shoots were also regenerated from explants of a 5-yr-old mature quandong tree. Excised in vitro-grown shoots produced roots in culture on a medium containing indolebutyric acid at pH 4 following shoot etiolation. These results offer the promise of clonal propagation in species in which conventional vegetative propagation by cuttings has not been reported, and will provide a useful adjunct to a quandong breeding program to develop edible fruit and nuts in semiarid areas.

scholarly journals Influence of propagation method a nd indole-3-butyric acid on growth and development of in vitro- and ex vitro-derived lingonberry plants

2006 ◽  
Vol 86 (1) ◽  
pp. 235-243 ◽  
Author(s):  
Samir C Debnath
Keyword(s):  
Butyric Acid ◽  
Growth And Development ◽  
Shoot Growth ◽  
Adventitious Shoot ◽  
Ex Vitro ◽  
Adventitious Shoot Regeneration ◽  
Propagation Methods ◽  
Rhizome Morphology ◽  
Rhizome Development

The effects of two propagation methods and four indole-3-butyric acid (IBA) concentrations were studied in two cultivars of lingonberry (Vaccinium vitis-idaea L.). Cultivars Regal and Erntedank, propagated by conventional softwood cuttings (SC) and adventitious shoot regeneration from excised leaves of micropropagated shoots (LC), were evaluated for growth and morphology. Significant two- and three-way interactions for shoot and rhizome characteristics were observed among the treatments. The LC plants produced shorter and less-vigorous shoots but had more stems, branches, leaves and rhizomes in contrast to conventional cuttings, which rarely produced rhizomes. IBA had an effect on shoot and rhizome morphology, but not on the frequency of rhizome formation. In vitro culture on zeatin-containing nutrient medium apparently induces the juvenile branching characteristics that favored enhanced rhizome production. The advantage of shoot and rhizome production of adventitiously produced LC plants over SC plants varied between the genotypes. Erntedank plants had better shoot growth and rhizome development across propagation methods than did Regal plants. Key words: Vaccinium vitis-idaea, propagation, regeneration, cuttings, indole-3-butyric acid

REGENERATION OF PLANTLETS FROM HYPOCOTYL DERIVED CALLUS OF MORUS ALBA

1995 ◽  
Vol 43 (3) ◽  
pp. 259-262 ◽  
Author(s):  
K. Kathiravan ◽  
A. Shajahan ◽  
A. Ganapathi
Keyword(s):  
Indoleacetic Acid ◽  
Adventitious Shoot ◽  
Morus Alba ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Shoot Buds ◽  
Adventitious Shoot Buds ◽  
Hypocotyl Segments

Plantlets were regenerated from hypocotyl callus of Morus alba cv. MR2. Calli were established from hypocotyl segments on Murashige and Skoog (MS) medium supplemented with indoleacetic acid (0.5 mg/1) and benzyladenine (BA) (0.5 mg/1). They were transferred to MS medium with different concentrations of naphthaleneacetic acid NAA and BA for four weeks. Adventitious shoot buds were observed by transferring callus onto fresh Linsmaier and Skoog (LS) medium containing NAA (0.5 mg/1) and BA (0.75 mg/1). Shoots produced in vitro were rooted on MS medium with indolebutyric acid (0.75 mg/1).

scholarly journals Adventitious Shoot Production from a Vireya Hybrid of Rhododendron

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 594-596 ◽  
Author(s):  
Giovanni Iapichino ◽  
Tony H.H. Chen ◽  
Leslie H. Fuchigami
Keyword(s):  
Acetic Acid ◽  
Adventitious Shoots ◽  
Fold Increase ◽  
Adventitious Shoot ◽  
In Vitro Rooting ◽  
Axillary Shoots ◽  
Shoot Production ◽  
Shoot Tip Explants ◽  
Indole 3 Acetic Acid

An efficient adventitious shoot production protocol has been developed for Rhododendron laetum × aurigeranum. Shoot tips taken from greenhouse-grown plants were cultured on Anderson's medium supplemented with 74 μM 2iP. Axillary shoots were excised and cultured on medium containing 23 μM IAA and 74 μM 2iP. After 6 months, brown callus developed at the cut surfaces of the shoot-tip explants. This callus produced many adventitious shoots (up to 70 per explant). Clusters of adventitious shoots were divided, subculture, and continued to proliferate shoots. An estimated 1600-fold increase in the number of shoots could be readily achieved in 6 months. In vitro rooting of adventitious shoots was accomplished in 4 weeks. Seventy-three percent of shoots rooted on 1/4 strength Anderson's medium supplemented with 28 μm IAA. Plantlet survival was 100%3 weeks after transfer to soil. Chemical names used: 1-H-indole-3-acetic acid (MA); N-(3 -methy1-2-butenyl) -1H-purine-6 amine (2iP).

scholarly journals Regeneration of Ornamental Cherry (Prunus) Taxa from Mature Stored Seed

HortScience ◽  
2000 ◽  
Vol 35 (4) ◽  
pp. 745-748 ◽  
Author(s):  
Karen E. Hokanson ◽  
Margaret R. Pooler
Keyword(s):  
Callus Formation ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Adventitious Shoot Regeneration ◽  
Potential System ◽  
Regeneration In Vitro ◽  
2,4 Dichlorophenoxyacetic Acid

Callus formation and adventitious shoot regeneration in vitro from mature stored seed were evaluated in eight ornamental cherry (Prunus) taxa: P. campanulata Maxim., P. maackii Rupr., P. sargentii Rehd., P. serrula Franch., P. serrulata Lindl., P. subhirtella Miq., P. virginiana L., and P. yedoensis Matsum. Several portions of the embryo (cotyledons and hypocotyl sections) and nine combinations of growth regulators (BA, 2,4-D, IBA, NAA, and TDZ) were compared. Effects of embryo portions and growth regulator treatments were generally small within taxa, but shoot formation differed among taxa. About 20% to 50% of the embryos from P. virginiana and P. serrula and ≈5% to 30% of those from P. maackii produced shoots. The other taxa generally did not produce shoots. Regeneration from mature stored seed in the responsive taxa represents a potential system for genetic transformation. Chemical names used: 6-benzyladenine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); indole-3-butyric acid (IBA); α-naphthaleneacetic acid (NAA); thidiazuron (TDZ).

scholarly journals Gelling Agents Affect Adventitious Shoot Organogenesis in White Ash

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 873E-873
Author(s):  
Sharon A. Bates ◽  
John E. Preece ◽  
John H. Yopp
Keyword(s):  
Liquid Medium ◽  
Shoot Growth ◽  
Shoot Organogenesis ◽  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Gelling Agent ◽  
White Ash ◽  
Shoot Initiation ◽  
Adventitious Shoot Organogenesis

Dissected white ash seeds were placed on an agar-solidified MS medium with 10 μM TDZ and 1 μM 2,4-D (shoot initiation medium). After 4 weeks, explants were transferred to shoot elongation medium (3 μM TDZ, 1 μM BA, and 1 μM IBA) solidified with 0.7% Sigma agar, 0.525% agar + 0.05% gelrite, 0.35% agar + 0.1% gelrite, 0.175% agar + 0.15% gelrite, 0.2% gelrite, or no gelling agent (liquid medium). After 12 weeks in vitro, shoot growth and number were suppressed in cultures containing 0.2% gelrite (9.3 mm and 0.7 shoots) and in cultures containing no gelling agent (6.9 mm and 0.7 shoots). There were no differences in shoot growth and number in cultures containing 0.7% Sigma agar (2.2 mm and 16.5 shoots), 0.525% agar + 0.05% gelrite (2.6 mm and 18.7 shoots), 0.35% agar + 0.1% gelrite (1.6 mm and 17.4 shoots), and 0.175% agar + 0.15% gelrite (2.0 mm and 20.4 shoots). The most vitrification occurred in cultures on medium with the lowest amount of agar, gelrite only, and liquid medium.

scholarly journals IN VITRO PROPAGATION OF GUAVA (PSIDIUM GUAJAVA L.) FROM SEEDLING EXPLANTS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 696e-696
Author(s):  
Yaseen Mohamed-Yaseen ◽  
Raymond J. Schnell ◽  
Robert J. Knight ◽  
T. L. Davenport
Keyword(s):  
Shoot Growth ◽  
Multiple Shoots ◽  
Psidium Guajava ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Seedling Explants ◽  
Cultivated Species ◽  
Regenerated Plantlets

Guava (Psidium guajava L.) is an exceptional source of vitamin. C. It is also considered to be the most important cultivated species of the Myrtel family. Shoot tip and stem node were taken from seedling germinated in Murashige and Skoog medium (MS) and cultured in the same medium supplemented with 1-3mg/l benzylaminopurine (BA) and 0.1mg/l naphthaleneacetic acid (NAA) or 0.2-2mg/l thidiazuron (TDZ) and 0.1mg/l NAA. Multiple shoots (4-6) were obtained in 4-5 weeks from culture in 1-2mg/l BA and 0.1mg/l NAA, while TDZ caused abnormal shoot growth. Shoots were rooted successfully with 100% frequency in MS medium containing 2mg/l indolebutyric acid and further elongation of shoots was achieved in MS medium, supplemented with lg/l activated charcoal. Regenerated plantlets were successfully established in soil.

scholarly journals Micropropagation of Antelope Bitterbrush [Purshia tridentata (Pursh) DC.]

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 312-314 ◽  
Author(s):  
John L. Edson ◽  
David L. Wenny ◽  
Annette Leege-Brusven
Keyword(s):  
Woody Plant ◽  
Naphthaleneacetic Acid ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Axillary Shoots ◽  
Full Strength ◽  
Woody Plant Medium ◽  
Half Strength ◽  
In Vitro Survival

In vitro—derived microshoots of antelope bitterbrush, incubated for 1 month in media supplemented with 0.44 μm BA, grew 0.8 and 1.1 cm longer in woody plant medium (WPM) compared to full-strength and half-strength Murashige and Skoog (MS) media, respectively. Explants cultured in WPM supplemented with 0.44 μm BA and 0.54 μm NAA produced a mean of five axillary shoots per explant. Explants dipped in 0.1% IBA or 0.1% NAA rooted best in 0.1% IBA with 89% success ex vitro vs. 60% success in vitro. Survival of acclimatized plantlets rooted ex vitro was 95%, while 50% survived when rooted in vitro. After 1 year of greenhouse growth, 98% of plantlets survived and flowered. Chemical names used: benzyladenine (BA), 3-indolebutyric acid (IBA), 1-naphthaleneacetic acid (NAA).

scholarly journals In Vitro Propagation of the Rare and Endangered Grevillea scapigera (Proteaceae)

HortScience ◽  
1992 ◽  
Vol 27 (3) ◽  
pp. 261-262 ◽  
Author(s):  
Eric Bunn ◽  
Kingsley W. Dixon
Keyword(s):  
Shoot Growth ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Zeatin Riboside ◽  
Multiplication Rate ◽  
Rare And Endangered Species ◽  
Isopentenyl Adenine ◽  
Rare And Endangered

Micropropagation, including adventitious shoot growth from leaf sections, was achieved for Grevillea scapigera (Proteaceae), a rare and endangered species from Western Australia. Shoot tips were initiated on filter paper supports with liquid WPM (Woody Plant Medium) and supplemented with 20 μm zeatin riboside and 2 μm GA3. Shoots were then incubated on WPM solidified with agar and supplemented with 5 μm kinetin and 0.5 μm BA, which produced an approximate 6-fold multiplication rate per month. Up to three adventitious shoots were induced from 0.7-cm2 leaf sections after 6 to 7 weeks on solid 1/2 MS (Murashige and Skoog) medium supplemented with 10 μm BA and 0.5 μm IBA. Shoots, 30 to 50 mm long, were rooted in vivo in a fogged glasshouse under 70% shade using a commerical rooting powder [IBA, 0.1% (w/w)] applied to the base of the shoots. Most (67%) of the shoots treated in this way rooted after 5 weeks. Established, rooted plants have been grown on under glasshouse conditions. Chemical names used: N6-[2-isopentenyl] adenine riboside (zeatin riboside); gibberellic acid (GA3); 6-furfurylaminopurine (kinetin); N-(phenylmethyl)-1H-purine-6-amine (BA); 1-H-indole-3-butyric acid (IBA).

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