Chromoplast Ultrastructure in Fruit of Solanum pseudocapsicum and Fruit and Sepals of Physalis alkekengi

1978 ◽  
Vol 26 (6) ◽  
pp. 793 ◽  
Author(s):  
DJ Simpson ◽  
MR Baqar ◽  
TH Lee
Keyword(s):  
The Other ◽  
Wild Plants ◽  
Ripe Fruit ◽  
Green Fruit ◽  
Different Types ◽  
Zeaxanthin Dipalmitate ◽  
Β Carotene ◽  
Chromoplast Ultrastructure

The chromoplasts of ripe fruit of Solanum pseudocapsicum contain mostly β-carotene, with lesser amounts of lutein, antheraxanthin, zeaxanthin, mutatochrome and violaxanthin. The ultrastructure of these plastids reveals that the carotenoids appear to crystallize out from the plastoglobules into many small slabs. In addition, fibrils are also present and develop from plastoglobules. In fruit from wild plants, they consist of a single microfibril with a diameter of 21.3 ± 1.4 nm ( ± SD), while those from an ornamental cultivar, Solanum Christmas Cherry Jubilee, contain fibrils consisting of several small microfibrils, with an average centre-to-centre distance of 4.9 ± 0.5 nm (±SD). The chromoplasts of ripe fruit of Physalis alkekengi are remarkably similar to those of S. pseudocapsicum with respect to their ultrastructure, although they contain mainly zeaxanthin dipalmitate and β-carotene. Fruit from one plant contain the electron-transparent crystalloids described for S. pseudocapsicum, as well as fibrils consisting of one or more large microfibrils. Fruit from a second plant contain crystalloids and the short, small fibrils composed of several microfibrils with a centre- to-centre distance of 4.1 ± 0.2 nm (± SD ) . Treatment of mature green fruit of S. pseudocapsicum with CPTA, which inhibits β-carotene accumulation, prevents the formation of crystalloids and both types of fibrils. It is suggested that the crystalloids are the site of β-carotene deposition in S. pseudocapsicum, and of zeaxanthin di- palmitate in P. alkekengi. Furthermore, it is postulated that there are different types of plasto- globules, one type generating crystalloids and the other fibrils.

Comparative Surface Imaging Study of Multifocal Diffractive Intraocular Lenses

2021 ◽  
Author(s):  
Hyeck Soo Son ◽  
Jung Min Lee ◽  
Ramin Khoramnia ◽  
Chul Young Choi
Keyword(s):  
Imaging Study ◽  
The Other ◽  
Intraocular Lenses ◽  
Optical Surface ◽  
Diffractive Optic ◽  
Atomic Force ◽  
Acceptable Range ◽  
Different Types ◽  
Concentric Pattern

Abstract Purpose To analyse and compare the surface topography and roughness of three different types of diffractive multifocal IOLs. Methods Using scanning electron microscope (SEM, Inspect F, 5.0 KV, maximum magnification up to 20,000) and atomic force microscope (AFM, Park Systems, XE-100, non-contact, area profile comparison, 10 × 10 µm, 40 × 40 µm), the surface quality of the following diffractive IOLs was studied: the AcrySof IQ PanOptix (Alcon, USA), the AT LARA 829MP (Carl Zeiss Meditec, Germany), and Tecnis Symfony (Johnson&Johnson Vision, USA). The measurements were made over three representative areas (central non-diffractive optic, central diffractive optic, and diffractive step) of each IOL. Roughness profile in terms of mean arithmetic roughness (Ra) and root-mean-squared roughness (Rq) values were obtained and compared statistically. Results In SEM examination, all IOLs showed a smooth optical surface without any irregularities at low magnification. At higher magnification, Tecnis Symfony showed unique highly regular, concentric, and lineate structures in the diffractive optic area which could not be seen in the other studied diffractive IOLs. The differences in the measured Ra and Rq values of the Tecnis Symfony were statistically significant compared to the other models (p < 0.05). Conclusion Various different topographical traits were observed in three diffractive multifocal IOLs. The Ra values of all studied IOLs were within an acceptable range. Tecnis Symfony showed statistically significant higher surface Ra values at both central diffractive optic and diffractive step areas. Furthermore, compared to its counterparts, Tecnis Symfony demonstrated highly ordered, concentric pattern in its diffractive surfaces.

RADIATION EXPOSURE TO THE BACK WITH DIFFERENT TYPES OF APRONS

2021 ◽  
Author(s):  
Seung Wan Hong ◽  
Tae Won Kim ◽  
Jae Hun Kim
Keyword(s):  
Radiation Exposure ◽  
Effective Dose ◽  
Exposure Time ◽  
The Other ◽  
Lead Apron ◽  
Type Group ◽  
Air Kerma ◽  
Different Types ◽  
The Relationship

Abstract Physicians and nurses stand with their back towards the C-arm fluoroscope when using the computer, taking things out of closets and preparing drugs for injection or instruments for intervention. This study was conducted to investigate the relationship between the type of lead apron and radiation exposure to the backs of physicians and nurses while using C-arm fluoroscopy. We compared radiation exposure to the back in the three groups: no lead apron (group C), front coverage type (group F) and wrap-around type (group W). The other wrap-around type apron was put on the bed instead of on a patient. We ran C-arm fluoroscopy 40 times for each measurement. We collected the air kerma (AK), exposure time (ET) and effective dose (ED) of the bedside table, upper part and lower part of apron. We measured these variables 30 times for each location. In group F, ED of the upper part was the highest (p &lt; 0.001). ED of the lower part in group C and F was higher than that in group W (p = 0.012). The radiation exposure with a front coverage type apron is higher than that of the wrap-around type and even no apron at the neck or thyroid. For reducing radiation exposure to the back of physician or nurse, the wrap-around type apron is recommended. This type of apron can reduce radiation to the back when the physician turns away from the patient or C-arm fluoroscopy.

scholarly journals MITOTIC CROSSING OVER AND NONDISJUNCTION IN TRANSLOCATION HETEROZYGOTES OF ASPERGILLUS

Genetics ◽  
1976 ◽  
Vol 82 (4) ◽  
pp. 605-627
Author(s):  
Etta Käfer
Keyword(s):  
Selective Advantage ◽  
The Other ◽  
Translocation Chromosome ◽  
Crossing Over ◽  
Selective Marker ◽  
Reciprocal Translocations ◽  
Poor Growth ◽  
Different Types ◽  
Diploid Genotype ◽  
One Step

ABSTRACT To analyze mitotic recombination in translocation heterozygotes of A. nidulans two sets of well-marked diploids were constructed, homo- or heterozygous for the reciprocal translocations T1(IL;VIIR) or T2(IL;VIIIR) and heterozygous for selective markers on IL. It was found that from all translocation heterozygotes some of the expected mitotic crossover types could be selected. Such crossovers are monosomic for one translocated segment and trisomic for the other and recovery depends on the relative viabilities of these unbalanced types. The obtained segregants show characteristically reduced growth rates and conidiation dependent on sizes and types of mono- and trisomic segments, and all spontaneously produce normal diploid sectors. Such secondary diploid types either arose in one step of compensating crossing over in the other involved arm, or—more conspicuously—in two steps of nondisjunction via a trisomic intermediate.—In both of the analyzed translocations the segments translocated to IL were extremely long, while those translocated from IL were relatively short. The break in I for T1(I;VII) was located distal to the main selective marker in IL, while that of T2(I;VIII) had been mapped proximal but closely linked to it. Therefore, as expected, the selected primary crossover from the two diploids with T2(I;VIII) in coupling or in repulsion to the selective marker, showed the same chromosomal imbalance and poor growth. These could however be distinguished visually because they spontaneously produced different trisomic intermediates in the next step, in accordance with the different arrangement of the aneuploid segments. On the other hand, from diploids heterozygous for T1(I;VII) mitotic crossovers could only be selected when the selective markers were in coupling with the translocation; these crossovers were relatively well-growing and produced frequent secondary segregants of the expected trisomic, 2n+VII, type. For both translocations it was impossible to recover the reciprocal crossover types (which would be trisomic for the distal segments of I and monosomic for most of groups VII or VIII) presumably because these were too inviable to form conidia.—In addition to the selected segregants of expected types a variety of unexpected ones were isolated. The conditions of selection used favour visual detection of aneuploid types, even if these produce only a few conidial heads and are not at a selective advantage. For T2(I;VIII) these "non-selected" unbalanced segregants were mainly "reciprocal" crossovers of the same phenotype and imbalance as the selected ones. For T1(I;VII) two quite different types were obtained, both possibly originating with loss of the small VII-Itranslocation chromosome. One was isolated when the selective marker in repulsion to T1(I;VII) was used and, without being homo- or hemizygous for the selective marker, it produced stable sectors homozygous for this marker. The other was obtained from both coupling and repulsion diploids and showed a near-diploid genotype; it produced practically only haploid stable sectors of the type expected from monosomics, 2n-1 for the short translocation chromosome.

scholarly journals Development of Speech Recognition Systems in Emergency Call Centers

Symmetry ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 634
Author(s):  
Alakbar Valizada ◽  
Natavan Akhundova ◽  
Samir Rustamov
Keyword(s):  
Speech Recognition ◽  
Markov Model ◽  
Hidden Markov ◽  
Call Centers ◽  
The Other ◽  
Language Models ◽  
Emergency Call ◽  
Acoustic Model ◽  
Different Types ◽  
Recognition Systems

In this paper, various methodologies of acoustic and language models, as well as labeling methods for automatic speech recognition for spoken dialogues in emergency call centers were investigated and comparatively analyzed. Because of the fact that dialogue speech in call centers has specific context and noisy, emotional environments, available speech recognition systems show poor performance. Therefore, in order to accurately recognize dialogue speeches, the main modules of speech recognition systems—language models and acoustic training methodologies—as well as symmetric data labeling approaches have been investigated and analyzed. To find an effective acoustic model for dialogue data, different types of Gaussian Mixture Model/Hidden Markov Model (GMM/HMM) and Deep Neural Network/Hidden Markov Model (DNN/HMM) methodologies were trained and compared. Additionally, effective language models for dialogue systems were defined based on extrinsic and intrinsic methods. Lastly, our suggested data labeling approaches with spelling correction are compared with common labeling methods resulting in outperforming the other methods with a notable percentage. Based on the results of the experiments, we determined that DNN/HMM for an acoustic model, trigram with Kneser–Ney discounting for a language model and using spelling correction before training data for a labeling method are effective configurations for dialogue speech recognition in emergency call centers. It should be noted that this research was conducted with two different types of datasets collected from emergency calls: the Dialogue dataset (27 h), which encapsulates call agents’ speech, and the Summary dataset (53 h), which contains voiced summaries of those dialogues describing emergency cases. Even though the speech taken from the emergency call center is in the Azerbaijani language, which belongs to the Turkic group of languages, our approaches are not tightly connected to specific language features. Hence, it is anticipated that suggested approaches can be applied to the other languages of the same group.

Plausibility and Aesthetic Interpretation

1977 ◽  
Vol 7 (2) ◽  
pp. 327-340 ◽  
Author(s):  
Denis Dutton
Keyword(s):  
Scientific Inquiry ◽  
The Other ◽  
Inquiry Science ◽  
Works Of Art ◽  
Different Types ◽  
Logical Structures ◽  
The One

If a catalogue were made of terms commonly used to affirm the adequacy of critical interpretations of works of art, one word certain to be included would be “plausible.” Yet this term is one which has received precious little attention in the literature of aesthetics. This is odd, inasmuch as I find the notion of plausibility central to an understanding of the nature of criticism. “Plausible” is a perplexing term because it can have radically different meanings depending on the circumstances of its employment. ln the following discussion, I will make some observations about the logic of this concept in connection with its uses in two rather different contexts: the context of scientific inquiry on the one hand, and that of aesthetic interpretation on the other. In distinguishing separate senses of “plausible,” I shall provide reason to resist the temptation to imagine that because logical aspects of two different types of inquiry—science and criticism—happen to be designated by the same term, they may to that extent be considered to have similar logical structures.

Comparison of two types of inocula during acclimation and stable operation for nitrophenol biodegradation in an anaerobic-aerobic SBR

2006 ◽  
Vol 54 (10) ◽  
pp. 39-45
Author(s):  
A. Vargas ◽  
D. González ◽  
A. Estival ◽  
G. Buitrón
Keyword(s):  
Wastewater Treatment ◽  
Treatment Plant ◽  
The Other ◽  
Stable Operation ◽  
Reaction Phase ◽  
Toxic Compound ◽  
Anaerobic Wastewater Treatment ◽  
Significant Difference ◽  
Different Types ◽  
Batch Bioreactors

This work presents a comparison of two inocula used for the acclimation of two anaerobic-aerobic sequencing batch bioreactors used for toxic wastewater treatment. The bioreactors were acclimated with different types of sludge: one coming from an anaerobic wastewater treatment plant and the other one from a conventional aerobic activated sludge plant. The model toxic compound was p-nitrophenol, which is reduced to p-aminophenol during the initial anaerobic phase of the reaction, and later mineralized during a posterior aerated reaction phase. Biodegradation of the compounds was monitored using UV/Vis spectrophotometry. After acclimation stabilization of the sludge and of the process was also monitored. Results show that there is no significant difference in acclimation times and stability of the process between the two employed inocula, and thus an originally anaerobic inoculum presents no apparent advantage over a more easily accessible aerobic one.

scholarly journals Escherichia coli O157:H7 SURVIVAL IN TRADITIONAL AND LOW LACTOSE YOGURT DURING FERMENTATION AND COOLING PERIODS

2017 ◽  
Vol 18 (0) ◽  
Author(s):  
Camila Sampaio Cutrim ◽  
Raphael Ferreira de Barros ◽  
Robson Maia Franco ◽  
Marco Antonio Sloboda Cortez
Keyword(s):  
Escherichia Coli ◽  
The Other ◽  
Lactose Hydrolysis ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Gas Formation ◽  
Whole Milk ◽  
Milk Contamination ◽  
Different Types ◽  
Fermentation Period

Abstract The purpose of this study was to evaluate the behavior of E. coli O157:H7 during lactose hydrolysis and fermentation of traditional and low lactose yogurt. It also aimed to verify E. coli O157:H7 survival after 12 h of storage at 4 ºC ±1 ºC. Two different types of yogurts were prepared, two with whole milk and two with pre-hydrolyzed whole milk; in both groups one yogurt was inoculated with E. coli O157:H7 and the other one was not inoculated. The survival of E. coli and pH of yogurt were determined during fermentation and after 12-h refrigeration. The results showed that E. coli O157:H7 was able to grow during the fermentation period (from 4.34 log CFU.mL-1 to 6.13 log CFU.mL-1 in traditional yogurt and 4.34 log CFU.mL-1 to 6.16 log CFU.mL-1 in low lactose yogurt). The samples with E. coli O157:H7 showed gas formation and syneresis. Thus, E. coli O157:H7 was able to survive and grow during fermentation of traditional and low lactose yogurts affecting the manufacture technology. Moreover, milk contamination by E. coli before LAB addition reduces the growth of L. bulgaricus and S. thermophilus especially when associated with reduction of lactose content.

Occurrence of Bubbles in a Thin Wire at Low Reynolds Number

1992 ◽  
Vol 114 (2) ◽  
pp. 255-260 ◽  
Author(s):  
K. Sato
Keyword(s):  
Separation Zone ◽  
Reynolds Numbers ◽  
Cavitation Number ◽  
The Other ◽  
Laminar Separation ◽  
Low Reynolds Numbers ◽  
Thin Wires ◽  
Different Types ◽  
Low Reynolds ◽  
Air Diffusion

Thin wires of various diameters from 0.07 to 0.7 mm are examined about appearances and characteristics of bubble occurrence behind them in the range of low Reynolds numbers. The appearance of bubbles is very dependent on diameters of wires. Two different types of bubbles can be observed in the present experiment. One is a streamer-type bubble for smaller wires and the other is a small unspherical bubble for larger wires. The incipient and the desinent values of cavitation number also change greatly with the bubble types. The streamer-type bubble is related to the presence of laminar separation zone and the growth due to air diffusion. The small unspherical bubble can be mainly attributed to the motion of rolled-up vortices and the growth due to vaporization.

The Atomic Structure of the UO2 (111) Surface and the Effects of Additional Surface Oxygen Studied by Elevated Temperature STM

1998 ◽  
Vol 05 (01) ◽  
pp. 315-320 ◽  
Author(s):  
C. Muggelberg ◽  
M. R. Castell ◽  
G. A. D. Briggs ◽  
D. T. Goddard
Keyword(s):  
Elevated Temperature ◽  
Atomic Structure ◽  
Uranium Oxide ◽  
The Other ◽  
Surface Oxygen ◽  
Surface Termination ◽  
Sample Bias ◽  
Different Types ◽  
Stm Images ◽  
Mobile Oxygen

The structure of the UO 2+x (111) surface has been investigated by elevated temperature STM. Images of atomic terraces reveal two different types of surface termination. One of them corresponds to the stoichiometric UO 2 (111) surface and can be resolved atomically in empty state images above ~ 1.6 V sample bias. The observed (1 × 1) ordering is thought to be due to uranium states because its occurrence corresponds to the bottom of the empty uranium 5f band. On these terraces mobile oxygen forms a local [Formula: see text] superstructure. The other terrace type observed on top of the UO 2+x (111) surface is thought to be a phase of a higher uranium oxide which has grown epitaxially.

Manipulating fruit chloroplasts as a strategy to improve fruit quality

2013 ◽  
Author(s):  
Alan B. Bennett ◽  
Arthur A. Schaffer ◽  
Ilan Levin ◽  
Marina Petreikov ◽  
Adi Doron-Faigenboim
Keyword(s):  
Chloroplast Development ◽  
Tomato Fruit ◽  
Soluble Sugar ◽  
Fruit Size ◽  
Soluble Solids ◽  
Nucleotide Polymorphisms ◽  
Ripe Fruit ◽  
Green Fruit ◽  
Functional Alleles ◽  
Sugar Levels

The Original Objectives were modified and two were eliminated to reflect the experimental results: Objective 1 - Identify additional genetic variability in SlGLK2 and IPin wild, traditional and heirloom tomato varieties Objective 2 - Determine carbon balance and horticultural characteristics of isogenic lines expressing functional and non-functional alleles of GLKsand IP Background: The goal of the research was to understand the unique aspects of chloroplasts and photosynthesis in green fruit and the consequences of increasing the chloroplast capacity of green fruit for ripe fruit sugars, yield, flavor and nutrient qualities. By focusing on the regulation of chloroplast formation and development solely in fruit, our integrated knowledge of photosynthetic structures/organs could be broadened and the results of the work could impact the design of manipulations to optimize quality outputs for the agricultural fruit with enhanced sugars, nutrients and flavors. The project was based on the hypothesis that photosynthetic and non-photosynthetic plastid metabolism in green tomato fruit is controlled at a basal level by light for minimal energy requirements but fruit-specific genes regulate further development of robust chloroplasts in this organ. Our BARD project goals were to characterize and quantitate the photosynthesis and chloroplast derived products impacted by expression of a tomato Golden 2- like 2 transcription factor (US activities) in a diverse set of 31 heirloom tomato lines and examine the role of another potential regulator, the product of the Intense Pigment gene (IP activities). Using tomato Golden 2-like 2 and Intense Pigment, which was an undefined locus that leads to enhanced chloroplast development in green fruit, we sought to determine the benefits and costs of extensive chloroplast development in fruit prior to ripening. Major conclusions, solutions, achievements: Single nucleotide polymorphisms in the promoter, coding and intronicSlGLK2 sequences of 20 heirloom tomato lines were identified and three SlGLK2 promoter lineages were identified; two lineages also had striped fruit variants. Lines with striped fruit but no shoulders were not identified. Green fruit chlorophyll and ripe fruit soluble sugar levels were measured in 31 heirloom varieties and fruit size correlates with ripe fruit sugars but dark shoulders does not. A combination of fine mapping, recombinant generation, RNAseq expression and SNP calling all indicated that the proposed localization of a single locus IP on chr 10 was incorrect. Rather, the IP line harbored 11 separate introgressions from the S. chmielewskiparent, scattered throughout the genome. These introgressions harbored ~3% of the wild species genome and no recombinant consistently recovered the IP parental phenotype. The 11 introgressions were dissected into small combinations in segregating recombinant populations. Based on these analyses two QTL for Brix content were identified, accounting for the effect of increased Brix in the IP line. Scientific and agricultural implications: SlGLK2 sequence variation in heirloom tomato varieties has been identified and can be used to breed for differences in SlGLK2 expression and possibly in the green striped fruit phenotype. Two QTL for Brix content have been identified in the S. chmielewskiparental line and these can be used for increasing soluble solids contents in breeding programs. 

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