Differential responses of accessions of native Australian Nicotiana species to water stress

2018 ◽  
Vol 66 (3) ◽  
pp. 265 ◽  
Author(s):  
Khondoker M. G. Dastogeer ◽  
Hua Li ◽  
Krishnapillai Sivasithamparam ◽  
Michael G. K. Jones ◽  
Stephen J. Wylie
Keyword(s):  
Water Stress ◽  
Water Content ◽  
Relative Water Content ◽  
Dry Weight ◽  
Shoot Tip ◽  
Initial Symptom ◽  
Fresh Weight ◽  
Nicotiana Species ◽  
Water Stress Tolerance ◽  
Relative Water

Thirty-two accessions of four Nicotiana species (Nicotiana benthamiana Domin, Nicotiana occidentalis H.-M.Wheeler, Nicotiana simulans N. Burb. and Nicotiana umbratica N.T.Burb.) collected from wild plants in northern Australia were assessed for responses to water stress. Under moderate water stress conditions, shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, root : shoot ratio, and relative water content of leaves were significantly affected. However, the degree to which the accessions were affected varied considerably. Some accessions of N. simulans, N. benthamiana and N. occidentalis were significantly more affected by water stress than others. There was significant variation between accessions in leaf and shoot tip wilting times. Initial symptom expression (leaf wilting) was significantly delayed in three accessions of N. benthamiana, and in one accession of N. umbratica. The least water stress tolerant lines, two accessions each of N. benthamiana, N. occidentalis and N. simulans, exhibited advanced symptoms of water stress (shoot tip wilting) within 14–17 days of cessation of watering. This stage was significantly delayed in three accessions of N. benthamiana and two accessions N. occidentalis and one accession of each of N. simulans and N. umbratica, which showed tip wilting only after 21–24 days. There were variations among the accessions of same Nicotiana species on their tolerance to water stress. Plant responses to water stress could not be predicted from their plant biomass and leaf relative water content under well-watered conditions. Leaf chlorophyll content was variable under water stress, but did not correlate with water stress tolerance.

scholarly journals In vitro screening of rice genotypes using polyethylene glycol under drought stress

2016 ◽  
Vol 27 (2) ◽  
pp. 128-135 ◽  
Author(s):  
J Akte ◽  
S Yasmin ◽  
MJH Bhuiyan ◽  
F Khatun ◽  
J Roy ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Water Content ◽  
Relative Water Content ◽  
Dry Weight ◽  
Proline Accumulation ◽  
Shoot Length ◽  
Fresh Weight ◽  
Relative Water

Five rice varieties viz. Binadhan-4, Binadhan-5, Binadhan-6, Binadhan-10 and Iratom-24 were evaluated in vitro under different water stress conditions. Several parameters such as germination percentage, shoot length, root length, shoot-root ratio, fresh weight, dry weight, turgid weight, relative water content and proline accumulation were studied. Drought condition was created by MS medium supplemented with five treatments of PEG, with a control such as 0%, 1%, 2%, 3% and 4% of PEG. The highest germination (100%) was found in the variety Binadhan-10 under low water stress conditions induced by 1% PEG. Similarly, the highest percentage of germination was found in all varieties under control condition (0% PEG). The lowest percentage of germination was obtained in the variety Iratom-24. But under severe stress (4% PEG), the highest percentage of germination was found only in the variety Binadhan-10. Moreover, the variety Binadhan-10 was found to be the best at 4% PEG for shoot length, root length, shoot-root ratio, relative water content and also the best at 1% PEG for fresh weight, dry weight, turgid weight. Water stress decreased relative water content and increased proline accumulation in rice. The highest relative water content was recorded in the variety Binadhan-10 and the lowest value recorded in the variety Binadhan-5. The highest proline content was obtained from the binadhan-6 at the highest treatment (4% PEG). Binadhan-10 showed the best performance almost in all the parameters under drought stress because of its own nature of tolerancy.Progressive Agriculture 27 (2): 128-135, 2016

scholarly journals PENGARUH ASAM GIBERELAT (GA3) DAN KALIUM NITRAT (KNO3) SERTA INTERAKSINYA TERHADAP SENESCENCE BUNGA POTONG GERBERA PUTIH (Gerbera jamesonii).

2019 ◽  
Vol 5 (2) ◽  
pp. 42-46
Author(s):  
Maulidina Agustin ◽  
Martha Lulus Lande ◽  
Zulkifli Zulkifli ◽  
Tundjung Tripeni Handayani
Keyword(s):  
Water Content ◽  
Relative Water Content ◽  
Dry Weight ◽  
Cut Flowers ◽  
Fresh Weight ◽  
Significance Level ◽  
Simple Effect ◽  
Relative Water ◽  
Homogeneity Of Variance ◽  
Concentration Levels

The purpose of this study was to decide whether the mixture of KNO3 and GA3 solutions was more effective than a single solution of KNO3 or GA3 in maintaining the freshness of cut flowers. The study was conducted at the Botanical Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung from November - December 2017. The research was carried out in a 2 x 3 factorial experiment with reason A was the GA3 solution with 3 concentration levels: 0%, 0.5%, and 1%. Factor B is KNO3 solution with 2 concentration levels: 0% and 5%. The limits in this study were fresh weight, dry weight, relative water content, and total dissolved carbohydrate content of gerbera cut flowers, qualitative limits were the level of reducing sugars. Measurements were made 7 days after soaking cut flowers in KNO3 or GA3 solutions, and a mixture of both. Homogeneity of variance and analysis of variance were determined at a significant level of 5%. Simple effect of KNO3 at each concentration level of GA3 decide by F test at 5% significance level. The results showed that GA3 with a concentration of 0.25% and 0.5% increased the fresh weight and dry weight of Gerbera cut flowers. KNO3 0.5% much reduced the relative water content of Gerbera cut flowers. The interaction between GA3 and KNO3 does not much effect all freshness of cut flowers. From the results of the study concluded that the single treatment of GA3 was more effective than the combination treatment of GA3 and KNO3 in maintaining the freshness of Gerbera cut flowers.

scholarly journals Gibberellic Acid (GA3) and Potassium Nitrate (KNO3): The Influence and Interaction inThe Senescence Process in White Rosa Sp.

2019 ◽  
Vol 6 (1) ◽  
pp. 32-38
Author(s):  
Nur Jannah Cortesa ◽  
Martha Lulus Lande ◽  
Zulkifli Zulkifli ◽  
Tundjung Tripeni Handayani
Keyword(s):  
Water Content ◽  
Chlorophyll Content ◽  
Relative Water Content ◽  
Dry Weight ◽  
Total Chlorophyll ◽  
Cut Flowers ◽  
Fresh Weight ◽  
Total Carbohydrate ◽  
Relative Water ◽  
White Rose

The purpose of this study was to find out whether the combination of KNO3 and GA3 solutions were more effective than a single solution of KNO3 or GA3 in keeping the freshness of cut flowers. The research was conducted in Botanical Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences University of Lampung from November to December 2017. The experiment was conducted in 2 x 3 factorial experiment with factor A is GA3 solution with 3 concentration level: 0% w/v, 25% w/v, and 0.5% w/v. Factor B is KNO3 solution with 2 concentration levels: 0% w/v and 5% w/v. The parameters of this study were fresh weight, dry weight, relative water content, total chlorophyll content, total chlorophyll of leaves, and total carbohydrate content of rose cut flowers. Measurements were made 7 days after the immersion of cut flowers in KNO3 or GA3 solution, and mixed both. Homogeneity of variance and analysis of variance is determined at confidence level 5%. KNO3 simple effect at every level of GA3 concentration is determined by the F test at 5%. The results showed that there was no interaction between GA3 and KNO3 to fresh weight, dry weight, relative water content and chlorophyll content a, b and total white roses, but KNO3 reduced fresh weight of cut flowers by 48.15% and dry weight 58.60%. KNO3 increased the relative water content of white rose flowers by 6.85%. The results of the study concluded that the combination of GA3 and KNO3 is not effective to keep the freshness of white rose flowers cut.

scholarly journals Ameliorative Effect of CaCl2 on Growth, Membrane Permeability and Nutrient Uptake in Oryza sativa Grown at High NaCl Salinity

2014 ◽  
Vol 8 ◽  
pp. 14-22 ◽  
Author(s):  
D. Anbu ◽  
S. Sivasankaramoorthy
Keyword(s):  
Oryza Sativa ◽  
Water Content ◽  
Membrane Permeability ◽  
Calcium Chloride ◽  
Nutrient Solution ◽  
Relative Water Content ◽  
Dry Weight ◽  
Physiological Variables ◽  
Relative Water ◽  
Ameliorative Effect

A pot culture was carried out with Oryza sativa L. vari-Co-39, to investigate the effects of supplementary calcium chloride on plants grown at NaCl (50mM) concentration. Treatments were: (1) Control: nutrient solution alone (C); (2) nutrient solution plus 50mM sodium chloride (NaCl); (3) nutrient solution plus 10mM calcium chloride (CaCl2); (4) nutrient solution plus 15mM calcium chloride (CaCl2); (5) nutrient solution and 50 mM NaCl plus supplementary 10 mM CaCl2 (NaCl + CaCl2); and (6) 50 mM NaCl plus additional mixture of 15 mM CaCl2 in nutrient solution (NaCl + CaCl2). The plants grown under salt stress produced low dry weight and relative water content than those grown in standard nutrient solution and in CaCl2 alone. Supplemental calcium chloride added to nutrient solution containing salt significantly improved growth and relative water content. Membrane permeability increased with high NaCl application and these increases in root membrane permeability were decreased with supplementary Ca. The concentration of chloride (Cl) increases highly for all treatments. Sodium (Na) concentration in plant tissues increased in both shoots and roots at high NaCl treatment. Application of supplementary Ca lowered Na concentration. Concentrations of Ca. K and N were at deficient ranges in the plants grown at high NaCl levels and these deficiencies were corrected by supplementary Ca. The ameliorating effect of Ca on growth and physiological variables could reduce the negative effect of salinity of Oryza sativa L., plants.

scholarly journals Modifications in vapor diffusion resistence of leaves and water relations in barbados cherry plants under water stress

2001 ◽  
Vol 13 (1) ◽  
pp. 75-87 ◽  
Author(s):  
REJANE J. MANSUR C. NOGUEIRA ◽  
JOSÉ ANTÔNIO P. V. DE MORAES ◽  
HÉLIO ALMEIDA BURITY ◽  
EGÍDIO BEZERRA NETO
Keyword(s):  
Water Stress ◽  
Water Potential ◽  
Water Content ◽  
Relative Water Content ◽  
Leaf Temperature ◽  
Diffusive Resistance ◽  
Severe Water Stress ◽  
Relative Water ◽  
Proline Concentration ◽  
Prolonged Stress

Young sexually and assexually propagated Barbados cherry plants were submitted to water deficit (20 days without irrigation). During this period the accumulation of proline, water potential of branches, osmotic potential, the relative water content of leaves, the leaf diffusive resistance, the transpiration rate and leaf temperature in the cuvette were determined. In addition, photosynthetically active radiation (PAR) and vapor pressure deficit (VPD) were measured in the porometer cuvette. The concentration of proline for both types of plants began to increase on the fifth day without watering, and reached 38.1 times the concentration in the control plants grown from seeds and 26.4 times the concentration in grafted plants on the tenth day without watering. The lowest levels of leaf water potential in the plants suffering from severe water stress varied from -4.5 to -5.7 MPa, the lowest values being observed in the sexually propagated plants. These plants also showed the highest values for transpiration (0.9 mmol.m-2.s-1) and proline concentration (20.42 mg.g-1 DM), the lowest for relative water content of the leaves (38.4%) and diffusive resistance (940 s.m-1) at the end of the experiment. The Barbados cherry plants developed strategies for surviving drought, with differences between various characteristics, resulting from prolonged stress, which significantly influenced the parameters evaluated, with the exception of leaf temperature.

scholarly journals Comparison of Strawberry F. chilocnsis and F. ×ananassa in Response to Water Stress: I. Leaf Water Potential, Relative Water Content, and Gas Exchange Characteristics

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 601c-601
Author(s):  
Chuhe Chen ◽  
J. Scott Cameron ◽  
Stephen F. Klauer
Keyword(s):  
Water Stress ◽  
Gas Exchange ◽  
Water Potential ◽  
Water Content ◽  
Leaf Water Potential ◽  
Relative Water Content ◽  
Leaf Water ◽  
Severe Water Stress ◽  
Relative Water ◽  
Gas Exchange Characteristics

Leaf water potential (LWP), relative water content (RWC), gas exchange characteristics, and specific leaf weight (SLW) were measured six hours before, during, and after water stress treatment in F. chiloensis and F. ×ananassa grown in growth chambers. The leaves of both species showed significantly lower LWP and RWC as water stress developed. F. ×ananassa had consistency lower LWP under stressed and nonstressed conditions than F. chiloensis. F. ×ananassa had higher RWC under nonstressed conditions, and its RWC decreased more rapidly under water stress than F. chiloensis. In comparison to F. ×ananassa, F. chiloensis had significantly higher CO2 assimilation rate (A), leaf conductance (LC), and SLW, but not transpiration rate (Tr), under stressed and nonstressed conditions. LC was the most sensitive gas exchange characteristic to water stress and decreased first. Later, A and stomatal conductance were reduced under more severe water stress. A very high level of Tr was detected in F. ×ananassa under the most severe water stress and did not regain after stress recovery, suggesting a permanent damage to leaf. The Tr of F. chiloensis was affected less by water stress. Severe water stress resulted in higher SLW of both species.

scholarly journals EFEITOS DO DÉFICIT HÍDRICO EM PARÂMETROS FISIOLÓGICOS DE FOLHAS DE SORGO (Sorghum bicolor, L.)

Irriga ◽  
1998 ◽  
Vol 3 (3) ◽  
pp. 81-88
Author(s):  
Carlos Augusto Lima Porto ◽  
Antonio Evaldo Klar ◽  
José Vicente Vasconcelos
Keyword(s):  
Water Stress ◽  
Sorghum Bicolor ◽  
Water Potential ◽  
Water Content ◽  
Relative Water Content ◽  
Water Status ◽  
Soil Water Potential ◽  
Relative Water ◽  
Conductivity Water ◽  
Sorghum Bicolor L

EFEITOS DO DÉFICIT HÍDRICO EM PARÂMETROS FISIOLÓGICOS DE FOLHAS DE SORGO (Sorghum bicolor, L.)  Carlos Augusto Lima PortoAntonio Evaldo Klar(2)José Vicente VasconcelosDepartamento de Engenharia Rural – Faculdade de Ciências Agronômicas – UNESPFone: (014) 821-3883  Fax: (014) 821-343818603-97’ – Botucatu - SP  1 RESUMO O experimento foi conduzido em casa de vegetação no Departamento de Engenharia Rural da Faculdade de Ciências Agronômicas - UNESP/Botucatu, SP, com delineamento experimental inteiramente casualizado, com 12 repetições. A cultura do sorgo (Sorghum bicolor, L.) foi plantada em vasos que continham 8,0 kg de solo (base em peso de solo seco), pertencente ao grande grupo Terra Roxa Estruturada para os dois tratamentos: a) plantas submetidas a défices  hídricos, sendo irrigadas quando o potencial de água no solo chegava a -1,5 MPa, elevando-o às imediações de -0,01 MPa), e b) plantas irrigadas constantemente por capilaridade. Todas as plantas foram irrigadas aos 55 dias após a emergência e os parâmetros avaliados foram: condutância estomática, potencial de água e teor relativo de água nas folhas mais novas totalmente expandidas, com determinações diárias entre as onze e treze horas, até que o potencial de água no solo atingisse valores em torno de -1,5 MPa. Da análise geral dos dados obtidos, pode-se inferir que a variação no status de água na folha observado através do potencial e do teor relativo de água nas folhas pode ser utilizado para indicar o momento de irrigar; ainda estas medições podem ser indicativas das plantas ou cultivares de sorgo que se mostram mais tolerantes à seca e que o mecanismo de adaptação é o  “avoidance”. UNITERMOS: Condutividade estomática, potencial de água na folha, teor relativo de água na folha,  tolerância à seca.  PORTO, C. A . L.., KLAR, A. E. , VASCONCELLOS, V. J.  Water deficit on physiological parameters of soybean  leaves (Sorghum bicolor L).  2 ABSTRACT A study was carried out at Agricultural Engineering Department, UNESP, Botucatu - SP, with a sorghum crop (Sorghum bicolor, L.) in order to physiologically evaluate the crop response to drought. A completely random design with twelve replications were used. Pots with 8 kg of a medium texture soil (dry weight basis) were used in order to test the influence of the two treatments: a) plants being submitted to a water stress, where irrigation were done when the water potential in the soil (s) were -1,5 MPa, raising it to about -0,01 MPa, and b) plants being always irrigated by capillary. The parameters evaluated were water vapor stomata conductivity, water potential  and relative water content in the leaves.  All plants were irrigated at 55 days after emergency, with daily determinations from eleven AM to thirteen PM, until soil water potential reaches around -1,5MPa. From the general data analysis, it can be inferred that there was a significant variation in the water status in the leaves by determinations of water potential and relative water content in the leaves, indicating that the method may be used to indicate the moment of irrigation and the plants and cultivars more tolerant to drought.  Sorghum plants showed adaptation to water stress under avoidance mechanism. KEYWORDS: Stomata conductivity, water potential in the leaves, relative water content, drought tolerance.

Effect of PEG mediated water stress on solute accumulation, relative water content, biomass and antioxidant enzymes in rice

2016 ◽  
Author(s):  
Ramadevi Kundur ◽  
Papi Reddy Reddy T. ◽  
Manohar Rao.T.
Keyword(s):  
Antioxidant Enzymes ◽  
Water Stress ◽  
Drought Tolerance ◽  
Water Content ◽  
Relative Water Content ◽  
Root Biomass ◽  
Proline Accumulation ◽  
Rice Varieties ◽  
Breeding Programs ◽  
Relative Water

In rice, several cultivated and upland varieties need to be assessed and analyzed for drought tolerance traits which could be used in screening and breeding programs for drought tolerance. Hence, the objectives of this study were to investigate the effects of water deficits in two rice cultivars and thereby analyze the role of several physiological traits useful in rice breeding programs for drought tolerance. The rice varieties Tellahamsa (TH) and N22 were screened for tolerance to drought. A comparative study was done subjecting them to PEG mediated water stress. Accumulation of solutes, i.e., proline, total free aminoacids and sugars; biomass production, Relative Water Content (RWC) and the levels of antioxidant enzymes, viz., Catalase (CAT), Ascorbate Peroxidase (APX), Glutathione Reductase(GR), and Superoxide Dismutase(SOD) were analyzed in response to water stress. Maximum proline accumulation was seen within 24hrs of stress, after 10 days TH decreased its proline to one-third, whereas in N22 doubled. Although amino acids doubled within 24hrs, gradually they depleted in N22. This may be due to conversion of aminoacids into proline which could be the most compatible solute. Sugars increased within 24hrs, but were depleted in 10days in both. In TH, the shoot and root biomass decreased, whereas in N22 there was a significant increase in root biomass. Shoot and root RWC of N22 was higher than TH under stress. GR increased in both TH and N22, APX and SOD increased only in N22. Proline accumulation, increase of root biomass and antioxidant enzymes such as APX or SOD during water stress are contributing to drought tolerance and could be used in screening for drought tolerance.

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