Inhibitory action of allelochemicals from Artemisia nanschanica to control Pedicularis kansuensis, an annual weed of alpine grasslands

2017 ◽  
Vol 65 (4) ◽  
pp. 305 ◽  
Author(s):  
Zhanhuan Shang ◽  
Yuan Hou ◽  
Fei Li ◽  
Cancan Guo ◽  
Tianhua Jia ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Weed Management ◽  
Inhibitory Action ◽  
Germination Rate ◽  
Chemical Compounds ◽  
Inhibitory Effect ◽  
The Tibetan Plateau ◽  
Inhibitory Effects ◽  
Alpine Pastures ◽  
Ethyl Acetate Extracts

The inhibitory action of allelochemicals can be used effectively for biological weed management and can minimise environmental impacts related to herbicides. The aim of the present study was to identify allelochemicals of Artemisia nanschanica Krasch, a typical weed with strong allelopathic effects, that could potentially control Pedicularis kansuensis Maxim, the key pioneer weed that causes rapid degradation of alpine pastures. Gas chromatography and mass spectrometry were used to identify the compounds of six extracts from A. nanschanica. Two ethyl acetate extracts were the most effective in reducing the seed germination rate and in inhibiting seedling shoot and root growth of P. kansuensis. Three chemical compounds from the ethyl acetate extracts were identified for their allelopathic inhibitory effects, a sesquiterpene and two aromadendrene oxides, with the sesquiterpene being the most effective. The three compounds showed the best inhibitory effect through synergistic action. We concluded that the three allelochemicals of A. nanschanica, either alone or in combination, can be used to biologically control P. kansuensis in alpine pastures on the Tibetan plateau.

Inhibition of ADP-Induced Responses in Human Platelets by Agents Elevating the Cyclic AMP Level: Comparison of Aggregation and Shape Change

1984 ◽  
Vol 52 (03) ◽  
pp. 333-335 ◽  
Author(s):  
Vider M Steen ◽  
Holm Holmsen
Keyword(s):  
Inhibitory Action ◽  
Human Platelet ◽  
Platelet Rich Plasma ◽  
Shape Change ◽  
Inhibitory Effect ◽  
Human Platelets ◽  
Inhibitory Effects ◽  
Early Step ◽  
Stimulus Response ◽  
Sequential Relationship

SummaryThe inhibitory effect of cAMP-elevating agents on shape change and aggregation in human platelets was studied to improve the understanding of the sequential relationship between these two responses.Human platelet-rich plasma was preincubated for 2 min at 37° C with prostaglandin E1 or adenosine, agents known to elevate the intracellular level of cAMP. Their inhibitory effects on ADP-induced shape change and aggregation were determined both separately and simultaneously. The dose-inhibition patterns for shape change and aggregation were similar for both PGE1 and adenosine. There was no distinct difference between the inhibitory action of these two inhibitors.These observations suggest that elevation of the intracellular concentration of cAMP interferes with an early step in the stimulus-response coupling that is common for aggregation and shape change.

Vasodilatory effects and underlying mechanisms of the ethyl acetate extracts from Gastrodia elata

2017 ◽  
Vol 95 (5) ◽  
pp. 564-571 ◽  
Author(s):  
Rong Dai ◽  
Ting Wang ◽  
Xiaoqin Si ◽  
Yuanyuan Jia ◽  
Lili Wang ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Vascular Tone ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Rat Aorta ◽  
Gastrodia Elata ◽  
Vasodilatory Effect ◽  
Intracellular Ca ◽  
Underlying Mechanisms ◽  
Ethyl Acetate Extracts

The objective of this study was to assess the ethyl acetate extracts of Gastrodia elata Blume (GEB) on vascular tone and the mechanisms involved. GEB was extracted with 95% EtOH followed by a further extraction with ethyl acetate. The effects of GEB and its ingredients on the isometric tensions of the aortic rings from rats were measured. The ethyl acetate extract of GEB induced a vasodilatory effect on rat aorta, which was partially dependent on endothelium. Four chemical compounds isolated from GEB were identified as 3,4-dihydroxybenzaldehyde (DB), 4-hydroxybenzaldehyde (HB), 4-methoxybenzyl alcohol (MA), and 4,4′-dihydroxydiphenyl methane (DM), respectively. All of these compounds induced vasodilatations, which were dependent on the endothelium to different degrees. After pretreatment with Nω-nitro-l-arginine methyl ester, indomethacin, or methylene blue, the vasodilatations induced by DB, HB, and MA were significantly decreased. In addition, the contractions of the rat aortic rings due to Ca2+ influx and intracellular Ca2+ release were also inhibited by DM. Furthermore, the administration of DB significantly enhanced the productions of nitric oxide (NO) and the activities of the endothelial NO synthase in aorta and in endothelial cells. Thus, GEB may play an important role in the amelioration of hypertension by modulating vascular tones.

scholarly journals Ethyl Acetate Extracts of Semen Impatientis Inhibit Proliferation and Induce Apoptosis of Human Prostate Cancer Cell Lines through AKT/ERK Pathways

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Tao Wang ◽  
Yang Cai ◽  
Wen Song ◽  
Ruibao Chen ◽  
Dunmei Hu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Western Blot ◽  
Ethyl Acetate ◽  
Caspase 3 ◽  
Inhibitory Effect ◽  
G1 Phase ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Impatiens Balsamina ◽  
Ethyl Acetate Extracts

Objective. To investigate the inhibitory effect of ethyl acetate extracts of Impatiens balsamina L. on prostate cancer cells. Methods. Impatiens balsamina L. was extracted to get water, ethanol, oil ether, ethyl acetate, and butanol extracts. CCK-8 assay was used to detect the inhibitory effect. Apoptosis rates and cell cycle distribution were detected by flow cytometry. Transwell assay was performed to test the ability of migration. The expressions of Bcl-2, Bax, cleaved-caspase-3, p-ERK, ERK, p-AKT, AKT, cyclin D1, cyclin E, and MMP2 were detected by Western blot. Results. Ethyl acetate extracts had the strongest inhibitory effect. After being treated with different concentrations of ethyl acetate extracts, the percentage of G0/G1 phase increased significantly, cyclin D1 and cyclin E expression decreased, apoptosis rate was significantly higher, and the ability of migration of PC-3 and RV1 was inhibited significantly. Western blot showed that the expressions of Bcl-2, p-ERK, and p-AKT were significantly decreased, but the expressions of Bax and caspase-3 cleavage were increased. Conclusions. Impatiens balsamina L. inhibited the proliferation of human prostate cancer cells; ethyl acetate extracts have the strongest effect. It could inhibit cell proliferation and migration, cause G1 phase arrest, and induce apoptosis probably through inhibition of the AKT and ERK pathways.

scholarly journals Profil Fitokimia Ekstrak Etil Asetat Temu Kunci (Boesenbergia rotunda L.) dan Serai (Cymbopogon citratus)

2021 ◽  
Vol 8 (1) ◽  
pp. 45
Author(s):  
Muhammad Priyadi ◽  
Nurul Chusna ◽  
Isnawati Isnawati ◽  
Opi Indriani
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Chemical Compounds ◽  
Cymbopogon Citratus ◽  
Color Reagent ◽  
Qualitative Test ◽  
Boesenbergia Rotunda ◽  
Layer Chromatography ◽  
Ethyl Acetate Extracts

Senyawa bahan alam yang terdapat pada tanaman memiliki banyak khasiat bagi kesehatan yang telah dibuktikan melalui pengobatan tradisional secara empiris. Identifikasi senyawa kimia sangat penting untuk mengetahui kemungkinan adanya senyawa yang dapat memiliki aktivitas farmakologi. Tanaman yang telah banyak digunakan oleh masyarakat termasuk pengobatan adalah temu kunci (Boesenbergia rotunda L.) dan serai (Cymbopogon citratus). Temu kunci dan serai diekstraksi menggunakan pelarut etil asetat. Uji fitokimia pada ekstrak etil asetat temu kunci dan serai dilakukan dengan uji kualitatif pereaksi warna dan pengendapan serta kromatografi lapis tipis untuk melihat gambaran pemisahan senyawa kimia yang terkandung. Ekstrak etil asetat temu kunci dan serai mengandung senyawa golongan alkaloid, terpenoid, flavonoid, fenol, dan kuinon. Senyawa pada temu kunci dan serai dapat dipisahkan menggunakan kromatografi lapis tipis dengan fase gerak etil asetat : n-heksan (8:2).    Kata Kunci: Fitokimia, Temu Kunci, Serai, Ekstrak Etil Asetat, Kromatografi Lapis Tipis Natural compounds found in plants have many health benefits that have been proven through empirically traditional medicine. Identification of chemical compounds is very important to determine the possibility of compounds having pharmacological activity. Plants that have been widely used by the community, including medicinal plants, are Temu Kunci (Boesenbergia rotunda L.) and serai or lemongrass (Cymbopogon citratus). Temu Kunci and lemongrass were extracted using ethyl acetate as a solvent. Phytochemical test on ethyl acetate extract of temu Kunci and lemongrass was carried out by qualitative test using color reagent and deposition and thin layer chromatography to see the description of the separation of the chemical compounds contained. Temu Kunci and lemongrass ethyl acetate extracts contain alkaloids, terpenoids, flavonoids, phenols, and quinones. Compounds in Temu Kunci and lemongrass can be separated using thin layer chromatography with ethyl acetate: n-hexane (8: 2) as mobile phase.

scholarly journals FOUR SOLVENT EXTRACTION OF CINNAMOMUM CAMPHORA XYLEM AND ANALYSIS OF THE ANTI-FUNGAL ACTIVITY OF THE EXTRACTIVES

Wood Research ◽  
2021 ◽  
Vol 66 (4) ◽  
pp. 666-677
Author(s):  
LI QUAN ◽  
NING JI ◽  
ZHANGUANG WANG ◽  
CUIXIANG LU ◽  
LIN HUI
Keyword(s):  
Ethyl Acetate ◽  
Chemical Compounds ◽  
Relative Content ◽  
Gas Chromatography Mass Spectrometry ◽  
Maximal Effect ◽  
Gloeophyllum Trabeum ◽  
Test Fungus ◽  
Growth Inhibitory ◽  
Anti Fungal ◽  
Ethyl Acetate Extracts

Four solvents including distilled water, acetone, ethyl acetate and petroleum ether were used to extract xylem of C. camphora. The differences in chemical compounds of xylem of C. camphorawere analyzed by gas chromatography/mass spectrometry(GC/MS)and the anti-fungal activityof C. camphoraextractives on Coriolus versicolor(CV), Trametes versicolor(TV),Poria vaporaria (PP) and Gloeophyllum trabeum(GT) were tested. The result showed that the chemical composition and relative content of the four different solvent extracts were different.A large number of chemical compounds in the C. camphoraextractives had a variety of biological activity and certain application value. The growth inhibitory rates of ethyl acetate extracts of C. camphora on PP, CV, TV and GTwere 52.24%, 52.51%, 43.26%, and 54.63%, respectively. According to the concentration for 50% of maximal effect, the inhibitory order on test fungus were GT > PP > CV > TV.

scholarly journals Potential and Persistence of the Inhibitory Effect of Sorghum on Weeds

2018 ◽  
Vol 36 (0) ◽  
Author(s):  
E.M. BIESDORF ◽  
L.D. PIMENTEL ◽  
M.F.F. TEIXEIRA ◽  
E. BIESDORF ◽  
P.H.H. SALLA ◽  
...  
Keyword(s):  
Weed Management ◽  
Block Design ◽  
Inhibitory Effect ◽  
Integrated Weed Management ◽  
Lower Amount ◽  
Inhibitory Effects ◽  
Weed Species ◽  
Inhibitory Interaction ◽  
Randomized Block Design ◽  
Weed Infestation

ABSTRACT: The inhibitory interaction between plants may be an important strategy of integrated weed management (IWM). This study aimed to investigate the inhibitory effects of sorghum on phytosociology and infestation of weeds during cultivation and after harvest. An experiment was carried out in the field in a randomized block design in a split-plot arrangement. Plots consisted of sorghum and corn (control) and subplots were composed of different periods of weed community assessment after crop harvest (DAH) (0, 20, 40, 60, and 80 days). An increased number of species and botanical families was observed after harvest. Degradation of sorghum and corn straw reached the stabilization at 60 DAH. Although sorghum has presented a lower amount of straw and soil cover, weed infestation was, on average, 30% lower in relation to areas cultivated with corn. Sorghum showed an inhibitory effect on some weed species, influencing phytosociology, especially during the first 40 DAH, and can be used as part of IWM strategy.

Inhibitory effects of antibiotics on platelet aggregation in vitro

1997 ◽  
Vol 16 (11) ◽  
pp. 662-666 ◽  
Author(s):  
Hiroko Kariyazono ◽  
Kazuo Nakamura ◽  
Terutoshi Shinkawa ◽  
Yukinori Moriyama ◽  
Hitoshi Toyohira ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Blood Concentration ◽  
Chemical Structure ◽  
Inhibitory Action ◽  
Adenosine Diphosphate ◽  
Inhibitory Effect ◽  
Carboxyl Group ◽  
Inhibitory Effects ◽  
Induced Aggregation

1 We evaluated in vitro inhibitory effects of six types of antibiotic, aztreonam (AZT), cefamandole (CMD), cefmetazole (CMZ), cefotiam (CTM), flomoxef (FMOX) and latamoxef (LMOX), on platelet aggregation, using healthy volunteers' blood. Four types-FMOX, LMOX, CTM and CMD-inhibited, in concentration of 2500 ?g/ml, the secondary aggregation induced by 3.0 ?M adenosine diphosphate (ADP), and also inhibited the aggregation induced by 0.5 ?g/ml collagen. AZT in the same concentration, did not inhibit the aggregation induced by collagen, and it inhibited only ADP induced aggregation. CMZ, in the same concentration, inhibited neither of the two aggregations. 2 The inhibitory effects of the antibiotics on collagen- induced aggregation were dependent on the concen tration of respective antibiotics. When classified by the strength of inhibitory action, LMOX and FMOX were strong, followed by CTM and CMD. The action of AZT and CMZ was weak. In particular, LMOX showed a 32% inhibitory effect at concentration of 50 ?g/ml, a level near the blood concentration obtained with clinical usual dose. 3 No relationship was observed between inhibitory effects of antibiotics on ADP- or collagen-induced aggregation and the presence or absence of carboxyl group and/or N-methyltetrazolethiol group in the chemical structure.

scholarly journals Ephedra alata extracts exhibits anti-obesity, anti-hyperlipidaemic, anti-hyperglycemia, anti-antipyretic and analgesic effects through the inhibition of lipase, α-amylase and inflammation

2020 ◽  
Author(s):  
Mohamed Tiss ◽  
Zoubeida Souiy ◽  
Mohamed Boujbiha ◽  
Lotfi Achour ◽  
Khaled Hamden
Keyword(s):  
Ethyl Acetate ◽  
Liver Toxicity ◽  
Ethyl Acetate Extract ◽  
Inhibitory Effect ◽  
Analgesic Effects ◽  
Fat Digestion ◽  
Key Enzyme ◽  
Analgesic Activities ◽  
First Time ◽  
Ethyl Acetate Extracts

Abstract In this present study, phytochemical screening, anti-obesity, anti- antipyretic and analgesic effect of Ephedra alata (EA) extracts were evaluated. Results of this study showed that the methanol EA extract (MEAE) had a stronger inhibition activity on key enzyme related to obesity as lipase (IC50 = 1.296 mg/ml) as compared to water and ethyl acetate extracts ( with IC50 = 1.639 and 1.897 respectively). Also results revealed that ethyl acetate extract depicted high activity toward key enzyme related to hyperglycemia as α-amylase (IC50 = 0.284 mg/mL) while water and methanol extracts showed good to moderate inhibitory activity (IC50 = 1,525 and 1,103 mg/ml respectively). In HFFD rats, the administration of MEAE at 200 mg/kg inhibited key enzyme related to fat digestion and absorption as lipase in the intestine, pancreas and serum by 53, 40 and 53% respectively,which leads to decrease in body weight by 20% and also modulation of lipid profile. In addition, the administration of MEAE to HFFD-rats exerts an inhibitory effect on α-amylase activity in the intestine, pancreas and serum by 43, 26 and 46% respectively and this leads to decrease in blood glucose level by 35%. Also, MEAE exert antipyretic and analgesic activities. This study demonstrates for the first time that MEAE are efficient in preventing obesity and hyperglycemia and liver toxicity. This data generated during study shows antipyretic and analgesic activities of MEAE.

scholarly journals SPATIAL SUMMATION OF INHIBITORY INFLUENCES IN THE EYE OF LIMULUS, AND THE MUTUAL INTERACTION OF RECEPTOR UNITS

1958 ◽  
Vol 41 (5) ◽  
pp. 1049-1066 ◽  
Author(s):  
H. K. Hartline ◽  
Floyd Ratliff
Keyword(s):  
Inhibitory Action ◽  
Spatial Summation ◽  
Nerve Impulse ◽  
Inhibitory Effect ◽  
Simultaneous Equations ◽  
The Other ◽  
Inhibitory Influence ◽  
Inhibitory Effects ◽  
Lateral Eye ◽  
Total Inhibition

The inhibitory influences exerted mutually among the receptor units (ommatidia) of the lateral eye of Limulus are additive. If two groups of receptors are illuminated together the total inhibition they exert on a "test receptor" near them (decrease in the frequency of its nerve impulse discharge in response to light) depends on the combined inhibitory influences exerted by the two groups. If the two groups are widely separated in the eye, their total inhibitory effect on the test receptor equals the sum of the inhibitory effects they each produce separately. If they are close enough together to interact, their effect when acting together is usually less than the sum of their separate effects, since each group inhibits the activity of the other and hence reduces its inhibitory influence. However, the test receptor, or a small group illuminated with it, may interact with the two groups and affect the net inhibitory action. A variety of quantitative effects have been observed for different configurations of three such groups of receptors. The activity of a population of n interacting elements is described by a set of n simultaneous equations, linear in the frequencies of the receptor elements involved. Applied to three interacting receptors or receptor groups equations are derived that account quantitatively for the variety of effects observed in the various experimental configurations of retinal illumination used.

In Vitro Effects of Ethanol on Rabbit Platelet Aggregation, Secretion of Granule Contents, and Cyclic AMP Levels in the Presence of Prostacyclin

1989 ◽  
Vol 61 (02) ◽  
pp. 254-258 ◽  
Author(s):  
Margaret L Rand ◽  
Peter L Gross ◽  
Donna M Jakowec ◽  
Marian A Packham ◽  
J Fraser Mustard
Keyword(s):  
Cyclic Amp ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Inhibitory Effects ◽  
Low Concentrations ◽  
Rabbit Platelets ◽  
High Concentrations ◽  
In Vitro Effects ◽  
Aggregation Of Platelets

SummaryEthanol, at physiologically tolerable concentrations, inhibits platelet responses to low concentrations of collagen or thrombin, but does not inhibit responses of washed rabbit platelets stimulated with high concentrations of ADP, collagen, or thrombin. However, when platelet responses to high concentrations of collagen or thrombin had been partially inhibited by prostacyclin (PGI2), ethanol had additional inhibitory effects on aggregation and secretion. These effects were also observed with aspirin- treated platelets stimulated with thrombin. Ethanol had no further inhibitory effect on aggregation of platelets stimulated with ADP, or the combination of ADP and epinephrine. Thus, the inhibitory effects of ethanol on platelet responses in the presence of PGI2 were very similar to its inhibitory effects in the absence of PGI2, when platelets were stimulated with lower concentrations of collagen or thrombin. Ethanol did not appear to exert its inhibitory effects by increasing cyclic AMP above basal levels and the additional inhibitory effects of ethanol in the presence of PGI2 did not appear to be brought about by further increases in platelet cyclic AMP levels.

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