Germination of mucilaginous seeds of Plantago albicans (Plantaginaceae): effects of temperature, light, pre-sowing treatments, osmotic stress and salinity

2014 ◽  
Vol 62 (2) ◽  
pp. 141 ◽  
Author(s):  
L. Veiga-Barbosa ◽  
F. Pérez-García

Plantago albicans L. (Plantaginaceae) is a perennial herbaceous plant widely distributed throughout the Mediterranean region. The germination requirements (under different controlled conditions of light and temperature, and after two pre-sowing treatments) and tolerance to osmotic stress (polyethylene glycol, PEG 6000) and salinity (NaCl) of P. albicans seeds were studied. Seeds were germinated under constant temperatures (5°C, 10°C, 15°C, 20°C, 25°C and 30°C) and alternating temperature regimes of 20/10°C and 25/15°C with a 16 h/8 h light/dark photoperiod. The outer layer of seeds become mucilaginous when wetted and the presence of mucilage on seeds significantly increased germination percentages at all temperatures tested. P. albicans seeds were non-dormant and temperature significantly affected germination percentages and germination rate (germination velocity expressed as mean germination time, MGT). The final germination percentages ranged from 34% to 89% for intact seeds (seeds with mucilage) and from 9% to 62% for demucilaged seeds, depending on the temperature. Temperatures of 25°C and 25/15°C gave the highest germination percentages. Light did not affect seed germination at both temperature regimes assayed (25°C and 25/15°C). Germination percentages of seeds soaked for 24 h in distilled water or in a gibberellic acid (GA3) solution were not significantly higher than that of untreated seeds. In general, both the final germination percentage and germination rate were reduced by increasing salinity and PEG concentration. Seeds germinated in up to 35% PEG and 300 mmol·L–1 NaCl. Recovery of germination for seeds when transferred to distilled water after being in PEG or salinity treatments for 15 days was quite high, suggesting that P. albicans seeds are tolerant to osmotic and salt stresses.

2017 ◽  
Vol 9 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Faride BEHBOUDI ◽  
Zeinalabedin TAHMASEBI SARVESTANI ◽  
Mohamad Zaman KASSAEE ◽  
Seyed Ali Mohamad MODARES SANAVI ◽  
Ali SOROOSHZADEH

Plants such as wheat and barley that are strategically important crops need to be considered to develop a comprehensive toxicity profile for nanoparticles (NPs). The present study was aimed to investigate the effects of chitosan and SiO2 NPs on wheat and barley plants. Two factorial experiments (seeds priming and direct exposure) were performed based on a completely randomized design in four replications. Results showed that the seeds priming with the NPs had not significant effect on germination parameters such as Germination Percentage (GP), Germination Rate (GR), Germination Value (GV), Mean Germination Time (MGT), Pick Value (PV) and Mean Daily Germination (MDG). In contrast, exposure of the seeds to the NPs had significant effects on these parameters. In both experiments, treatments had significant effects on shoot, seedling, root length, fresh and dry weight, as well as vigor indexes as compared to the control. In most traits, the best concentration of NPs was 30 ppm, whereas applications of the NPs with 90 ppm displayed adverse effects on majority of the studied traits. According to these results, selectivity in applications of NPs with suitable concentration and method is essential for different plant species.  


2018 ◽  
Vol 36 ◽  
Author(s):  
X. LIU ◽  
T. ZONG ◽  
Y. LI ◽  
X. ZHOU ◽  
L. BAI

ABSTRACT: Carolina geranium (Geranium carolinianum) is an emerging invasive weed in rape and wheat fields in China. A better understanding of its germination and emergence ecology will enable the development of integrated weed control strategies. In this study, we investigated the effects of temperature, photoperiod, soil water content, salinity, and burial depth, on germination and emergence of Carolina geranium. Germination percentages were over 74% under 15/20 and 20/25 oC night/day temperature regimes. Germination rate was independent of light/dark regime. Increasing salinity reduced germination of Carolina geranium from 81.1% at 0 mM to 0% at 160 mM NaCl. Seeds germination was peaked at 50% soil moisture, but was completely inhibited at < 20% and > 90%. The seedling emergence above 82.2% was observed when seeds were placed at a depth from 0 to 1 cm, and no seedlings emerged from seeds placed at a depth of 7 cm. Current work provide the basic information to effectively prevent and control this invasive weed in Chinese rape and wheat fields.


2019 ◽  
Vol 41 ◽  
pp. e46030
Author(s):  
João Everthon da Silva Ribeiro ◽  
Arliston Pereira Leite ◽  
Jackson Silva Nóbrega ◽  
Edna Ursulino Alves ◽  
Riselane de Lucena Alcântara Bruno ◽  
...  

Erythroxylum pauferrense is an endemic understory plant species of the Northeast Region of Brazil. The species is of great importance to the region and so ecophysiological studies are needed for its preservation. The objective of the present study was to determine the best substrates and temperatures for testing germination and seed vigor of E. pauferrense. An experiment was performed comprising a completely randomized design in a 5 x 4 factorial scheme, with five temperature regimes (20, 25, 30, 35°C constant and 20-30°C alternating) and four types of substrates (paper, vermiculite, sand and commercial substrate). The following characteristics were evaluated: germination percentage, first germination count, germination speed index, mean germination time, seedling length and dry mass (root and shoot). Paper and vermiculite substrates combined with constant temperatures of 20, 25, 30 and alternated between 20-30°C, provide greater seed germination and vigor while 35°C reduces seed physiological quality.


Botany ◽  
2013 ◽  
Vol 91 (1) ◽  
pp. 12-16 ◽  
Author(s):  
Esther Giménez Luque ◽  
Isabel C. Delgado Fernández ◽  
Francisco Gómez Mercado

Limonium cossonianum Kuntze is an interesting endemic plant in the southeastern Iberian Peninsula and the Balearic Islands that can be used in wetland restoration and as an ornamental species in saline areas. Experiments were carried out to investigate seed germination responses in L. cossonianum at different levels of salinity (0, 100, 200, and 400 mmol/L NaCl) and under different temperature regimes (20/10, 25/15, 30/20, and 35/25 °C, day/night), both in a 14 h light – 10 h dark photoperiod. Seed germination in L. cossonianum was significantly affected by salinity levels, temperature, and the interaction of both parameters. Maximum germination was recorded in the lowest saline media (100 mmol/L NaCl) and distilled water (0 mmol/L NaCl) at 20/10 °C. No seeds germinated in concentrations above 200 mmol/L NaCl at the higher temperatures. The increase in salinity delayed the beginning and end of germination, reduced final germination percentage, and increased mean time to germination. The rate of germination decreased with an increase in salinity and temperature.


Botany ◽  
2012 ◽  
Vol 90 (8) ◽  
pp. 731-742 ◽  
Author(s):  
Félix Pérez-García ◽  
Federico Varela ◽  
M. Elena González-Benito

Gentiana lutea L. (yellow gentian, Gentianaceae) is an important medicinal plant under protection as endangered species in most European countries. The aim of this work was to evaluate variation in seed mass, seed water content, and seed germination among 56 wild accessions of G. lutea. The effect of gibberellic acid (GA3), putrescine, moist chilling, and level of ripeness of seeds on subsequent germination was also investigated. Seeds of G. lutea showed physiological dormancy (final germination percentages ranged from 0% to 11%, depending on the accession) and GA3 enhanced seed germination drastically in all the accessions. The highest germination (99%) of GA3-treated seeds was reached at 15 °C. Final germination percentage and germination rate (as expressed by mean germination time), as well as seed mass and seed water content, varied significantly among accessions. In general, 1 year moist chilling did not significantly enhance G. lutea seed germination. For most accessions, no significant differences were found between fully ripe seeds and less ripe seeds for seed water content, seed mass, and seed germination. Applications of GA3 were always most effective than those of putrescine for increasing seed germination.


2021 ◽  
Vol 10 (2) ◽  
pp. e27110212471
Author(s):  
Aline Bueno Ramalho ◽  
Lúcia Filgueiras Braga

Plant growth regulators in the germination of Diospyros hispida A. DC. (Ebenaceae) Seed. Diospyros hispida species is a native fruit that has long germination period, hindering seedling production. The aim of this work was to evaluate the germination of Diospyros hispida seeds under the effect of gibberellic acid and biostimulant applications. Four replicates of 25 seeds per treatment were used. Treatments were: T1 - distilled water, T2, T3 and T4 - GA3 solutions at 100, 150 and 200 mg L-1, respectively, T5 - Immersion for 24 hours in distilled water with aeration, T6, T7 and T8 - Immersion for 24 hours in aerated GA3 solutions at 100, 150 and 200 mg L-1, respectively, T9, T10 and T11 - biostimulant application (containing auxin, gibberellin and cytokinin) at 3, 5 and 7 ml kg-1 of seeds. Germination rate, mean germination time, germination speed index, synchronization index, relative germination frequency, normal seedlings, root length, shoot length and seedling dry mass were evaluated. The effect of gibberellic acid and biostimulant containing auxin, gibberellin and cytokinin negatively influenced Diospyros hispida germination under the conditions of this study.


Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 577
Author(s):  
Mi-Hyun Lee ◽  
Jin-Hyun Lim ◽  
Cho-Hee Park ◽  
Jun-Hyeok Kim ◽  
Chae-Sun Na

In this study, we determined the germination response in the seeds of the rare plant Pseudolysimachion pusanensis (Y. N. Lee) Y. N. Lee to different temperatures. P. pusanensis seeds were collected from the Baekdudaegan National Arboretum, South Korea, in November 2019, and dried. Dry seeds were placed at constant and alternating temperatures (5 °C, 10 °C, 15 °C, 20 °C, 25 °C, 30 °C, and 35 °C) to determine their germination percentage (GP). The seeds were exposed to 59 temperature combinations ranging from 5 °C to 43 °C using a thermal gradient plate. The photoperiod was set at 12:12 h (light:dark) and germination assays were performed five times a week. Subsequently, the seed GP and the number of days required to reach 50% of the germination (T50) were determined. The highest final GP was 94.38%, with a T50 value of 9.26 d at 15 °C. However, the mean germination time was 12.5 d at 15 °C, and linear regression using 1/T50 revealed that the base temperature ranged from 2.69 °C to 4.68 °C. These results for P. pusanensis seeds stored in a seed bank provide useful data for the native plants horticulture industry and can also be utilized for storage management.


2012 ◽  
Vol 76 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Maryam Haghighi ◽  
Zahra Afifipour ◽  
Maryam Mozafarian

The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity StressThis study was conducted to evaluate the effectiveness of silicon (Si) application under salinity levels on seed germination and growth characteristics of tomato seeds. A laboratory experiment was performed on completely randomized design with two levels of salinity (25 and 50 mM NaCl) and 2 concentration of Si (1 and 2 mM) with 4 replications. Germination percentage, germination rate, seedling shoot and root length, fresh and dry weight of seedling and mean germination time was measured. Seed germination ofLycopersicon esculentumL. was significantly affected by salinity levels, Si and their interaction. Germination characteristics of tomato seeds decreased drastically by increasing NaCl concentrations. However, 1 mM Si had positive effects on seed germination characteristics and improved germination percentage, germination rate and mean germination time. Si alleviated the harmful effect of salinity stress on tomato seed germination at almost all germination characteristics.


2017 ◽  
Vol 40 (04) ◽  
Author(s):  
Hamdi Özaktan ◽  
Cemalettin Yasar Çiftçi Çiftçi ◽  
Mehmet Demir KAYA ◽  
Sati Uzun ◽  
Oguzhan Uzun ◽  
...  

The effects of chloride salts (NaCl, MgCl2 and CaCl2) with the same electrical conductivity (EC) values on germination, emergence, seedling growth and Cl content of seedling in chickpea cultivars (Akçin 91, Aziziye, Gökçe, Inci, Iºýk-05 and Yaºa-05) were investigated. The EC values of the chloride salts were adjusted to 4, 8 and 16 dS m-1 and the distilled water was served as control. Germination percentage, mean germination time, radicula and plumula lengths, fresh and dry radicula and plumula weight, chloride (Cl-) content of seedling in germination test, and seedling length, fresh and dry seedling weight in emergence test were measured. The results revealed that increasing salt doses caused a significant reduction in parameters of all cultivars. The dose of 16 dS m-1 from each salt was found to be lethal. Cl- content of seedling was increased from 0.05 ppm in control to 4.10 ppm in 16 dS m-1; resulting in decreasing germination, emergence and seedling growth. The cultivar Aziziye presented better performance and gave higher emergence rates in NaCl, while Iºýk-05 in CaCl2 and Gökçe in MgCl2; indicating that there was a genotypic variation towards different salt sources. It was concluded that emergence and seedling growth were severely influenced by increasing salinity without changing germination and emergence was not possible at 16 dS m-1.


2012 ◽  
Vol 47 (8) ◽  
pp. 1181-1184 ◽  
Author(s):  
Badar‑uz‑Zaman ◽  
Arshad Ali ◽  
Syed Ishtiaq Hyder ◽  
Muhammad Arshadullah ◽  
Saqib Umar Bhatti

The objective of this work was to determine if KCl could be a useful nutrient primer for safe seed germination in maize crop under salt stress conditions. Seed priming was done using 50 mmol L‑1 of muriate of potash, and germination and seedling growth were evaluated after salt stress with NaCl up to 50 mmol L‑1. Another set of seeds was tested under the same salt stress conditions without priming. Under salinity stress, germination percentage, germination rate index, germination coefficient, and seedling vigor indexes were higher in primed seeds. In unprimed seeds, mean germination time increased, while the germination rate index and the fresh and dry matter mass decreased more sharply with salinity stress. The Na/K ratio was higher in unprimed seeds.


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