Genomic constitution and phylogenetic position of several New Zealand Triticeae species revealed by two single copy nuclear genes

2011 ◽  
Vol 59 (1) ◽  
pp. 1 ◽  
Author(s):  
Aimee G. Oliver ◽  
Kara Harnish ◽  
Genlou Sun
Keyword(s):  
New Zealand ◽  
North American ◽  
Diploid Species ◽  
Single Copy ◽  
Nuclear Genes ◽  
Phylogenetic Position ◽  
Genome Constitution ◽  
Elymus Species ◽  
New Zealand Flora ◽  
Hordeum Species

Three genera of Triticeae, Elymus, Stenostachys and Australopyrum, are described in the New Zealand flora. Cytological analyses suggested that five basic genomes (St, H, Y, P and W) donated by different diploid species in different combinations exist in the genera Elymus and Stenostachys, whereas Australopyrum species contain the W genome only. Morphological and cytogenetic data suggested that the genome constitution for both E. apricus and E. multiflorus is StYW. Chloroplast DNA and ITS data supported the genome constitution of these Elymus species, but the HW genome constitution was assigned to the Stenostachys species. In this study, sequences of two single copy nuclear genes, RPB2 and DMC1, were used to confirm or refute the genome constitutions of the two Stenostachys species and the two Elymus species from New Zealand, and to analyse their phylogenetic relationships with other Elymus species. Our RPB2 and DMC1 data confirmed that the genome constitution of hexaploid E. apricus is StWY, and tetraploid S. gracilis is HW. The presence of the StW genome in hexaploid E. multiflorus, and the W genome in tetraploid S. laevis is also confirmed. No obvious St genome differentiation between New Zealand and non-New Zealand species is observed. The H genomes in the S. gracilis and S. laevis are closely related to the H genome from North American species, indicating that the H genomes in these two New Zealand species might originate from North American Hordeum species.

Origin of the H genome in StH-genomic Elymus species based on the single-copy nuclear gene DMC1

Genome ◽  
2011 ◽  
Vol 54 (8) ◽  
pp. 655-662 ◽  
Author(s):  
Genlou Sun ◽  
Xiaodi Zhang
Keyword(s):  
North American ◽  
Diploid Species ◽  
Nuclear Gene ◽  
Single Copy ◽  
Elymus Lanceolatus ◽  
Single Copy Nuclear Gene ◽  
Elymus Species ◽  
Elymus Multisetus ◽  
Elymus Hystrix ◽  
Hordeum Species

Previous studies have suggested that the H haplome in Elymus could originate from different diploid Hordeum species, however, which diploid species best represent the parental species remains unanswered. The focus of this study seeks to pinpoint the origin of the H genome in Elymus. Allopolyploid Elymus species that contain the StH genome were analyzed together with diploid Hordeum species and a broad sample of diploid genera in the tribe Triticeae using DMC1 sequences. Both parsimony and maximum likelihood analyses well separated the American Hordeum species, except Hordeum brachyantherum subsp. californicum, from the H genome of polyploid Elymus species. The Elymus H-genomic sequences were formed into different groups. Our data suggested that the American Horedeum species, except H. brachyantherum subsp. californicum, are not the H-genomic donor to the Elymus species. Hordeum brevisubulatum subsp. violaceum was the progenitor species to Elymus virescens, Elymus confusus, Elymus lanceolatus, Elymus wawawaiensis, and Elymus caninus. Furthermore, North American H. brachyantherum subsp. californicum was a progenitor of the H genome to Elymus hystrix and Elymus cordilleranus. The H genomes in Elymus canadensis, Elymus sibiricus, and Elymus multisetus were highly differentiated from the H genome in Hordeum and other Elymus species. The H genome in both North American and Eurasian Elymus species was contributed by different Hordeum species.

Origins and chromosome differentiation of Thinopyrum elongatum revealed by PepC, Pgk1 genes and ND-FISH

Genome ◽  
2021 ◽  
Author(s):  
Yi Dai ◽  
Shuai Huang ◽  
Genlou Sun ◽  
Haifeng Li ◽  
Shiqiang Chen ◽  
...  
Keyword(s):  
Gene Pool ◽  
Genetic Improvement ◽  
Phylogenetic Analyses ◽  
Single Copy ◽  
Nuclear Genes ◽  
Chromosome Differentiation ◽  
Genome Constitution ◽  
Thinopyrum Elongatum ◽  
Intraspecific Relationships

<i>Thinopyrum elongatum</i> is an important gene pool for wheat genetic improvement. However, the origins of the <i>Thinopyrum</i> genomes and the nature of the genus’ intraspecific relationships are still controversial. In this study, we used single-copy nuclear genes and non-denaturing fluorescence <i>in situ</i> hybridization (ND-FISH) to characterize genome constitution and chromosome differentiation in <i>Th. elongatum</i>. According to phylogenetic analyses based on <i>PepC</i> and <i>Pgk1</i> genes, there was an E genome with three versions (E<sup>e</sup>, E<sup>b</sup>, E<sup>x</sup>) and St genomes in the polyploid <i>Th. elongatum</i>. The ND-FISH results of pSc119.2 and pAs1 revealed that the karyotypes of diploid <i>Th. elongatum</i> and <i>Th. bessarabicum</i> were different and the chromosome differentiation occurred among accessions of the diploid <i>Th. elongatum</i>. In addition, the tetraploid <i>Th. elongatum</i> has two groups of ND-FISH karyotype, indicating that the tetraploid <i>Th. elongatum</i> might be a segmental allotetraploid. In summary, our results suggested that the diploid <i>Th. elongatum</i>, <i>Th. bessarabicum</i> and <i>Pseudoroegneria</i> were the donors of the E<sup>e</sup>, E<sup>b</sup> and St genome to polyploid <i>Th. elongatum</i> species, respectively.

scholarly journals Genome analysis of South American Elymus (Triticeae) and Leymus (Triticeae) species based on variation in repeated nucleotide sequences

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 505-520 ◽  
Author(s):  
Jorge Dubcovsky ◽  
A. R. Schlatter ◽  
M. Echaide
Keyword(s):  
Genome Analysis ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Nucleotide Sequences ◽  
South American ◽  
Restriction Fragments ◽  
Genome Constitution ◽  
Elymus Species ◽  
Hordeum Species ◽  
Species Hybridization

Variation in repeated nucleotide sequences (RNSs) at the level of entire families assayed by Southern blot hybridization is remarkably low within species and is a powerful tool for scrutinizing the origin of allopolyploid taxa. Thirty-one clones from RNSs isolated from different Triticeae genera were used to investigate the genome constitution of South American Elymus. One of these clones, pHch2, preferentially hybridized with the diploid H genome Hordeum species. Hybridization of this clone with a worldwide collection of Elymus species with known genome formulas showed that pHch2 clearly discriminates Elymus species with the H genome (StH, StHH, StStH, and StHY) from those with other genome combinations (StY, StStY, StPY, and StP). Hybridization with pHch2 indicates the presence of the H genome in all South American Elymus species except Elymus erianthus and Elymus mendocinus. Hybridization with additional clones that revealed differential restriction fragments (marker bands) for the H genome confirmed the absence of the H genome in these species. Differential restriction fragments for the Ns genome of Psathyrostachys were detected in E. erianthus and E. mendocinus and three species of Leymus. Based on genome constitution, morphology, and habitat, E. erianthus and E. mendocinus were transferred to the genus Leymus.Key words: Triticeae, Elymus, Leymus, repeated sequences.

Molecular phylogeny and reticulate origins of several American polyploid Hordeum species

Botany ◽  
2011 ◽  
Vol 89 (6) ◽  
pp. 405-415 ◽  
Author(s):  
Huan Wang ◽  
Genlou Sun
Keyword(s):  
Diploid Species ◽  
Nuclear Gene ◽  
Single Copy ◽  
Polyploid Species ◽  
Sequence Comparisons ◽  
Internal Transcribed Spacer Sequences ◽  
Hordeum Jubatum ◽  
Terminal Element ◽  
Hordeum Species ◽  
Genome Donors

The phylogeny of diploid species in the genus Hordeum has been studied intensively. Although the origin of American polyploid species has been analyzed using multiple-copy internal transcribed spacer sequences, the origins of these species in Hordeum remain unclear. The objectives of our study were to elucidate the origins of American polyploid species and to explore phylogenetic relationships of these polyploids to diploid Hordeum and other diploid species in Triticeae using a single copy of a nuclear gene, disrupted meiotic cDNA1 (DMC1). DMC1 sequences from nine Hordeum polyploid species were analyzed. Sequence comparisons revealed that one copy of sequences from polyploid species Hordeum fuegianum , Hordeum jubatum , and Hordeum tetraploidum showed a 82 bp miniature inverted-repeat terminal element (MITE) (Stowaway) insertion, which was also detected in the Triticeae diploid species Australopyrum species (W genome) and Taeniatherum caput-medusae (Ta genome). Maximum parsimony and Bayesian analysis suggested that diploid Hordeum brachyantherum subsp. californicum is one ancestor of polyploids Hordeum arizonicum , H. brachyantherum subsp. brachyantherum , Hordeum depressum , and Hordeum procerum . The other ancestor of tetraploid H. depressum is probably Hordeum euclaston . Hordeum cordobense was suggested to be one of the genome donors to hexaploid H. procerum. The diploid Hordeum flexuosum and tetraploid H. tetraploidum were suggested as the parents to hexaploid species Hordeum parodii . The result is that one sequence from each of three Hordeum tetrapolyploids, including H. fuegianum, H. jubatum, and H. tetraploidum, and one from Hordeum hexaploid H. arizonicum fall outside the Hordeum clade of the DMC1 phylogenetic tree, therefore representing another example of complex evolutionary history. Our data may shed light on future phylogenetic studies in Triticeae, especially for the polyploids, by broadening the scope of investigations through sampling more genome types in Poaceae, not only from the tribe Triticeae.

He awa whiria—A “Braided River”: An Indigenous Māori Approach to Mixed Methods Research

2021 ◽  
pp. 155868982098402
Author(s):  
Rhiannon Martel ◽  
Matthew Shepherd ◽  
Felicity Goodyear-Smith
Keyword(s):  
Mixed Methods ◽  
New Zealand ◽  
North American ◽  
Mixed Methods Research ◽  
Mixed Methodology ◽  
Braided River ◽  
Research Framework ◽  
Research Philosophy

While literature on mixed methodology predominantly focuses on North American and European philosophical stances, non-Eurocentric worldviews and indigenous philosophies are also relevant to mixed methods research. This article aims to present the indigenous Māori worldview ( te ao Māori) and how this lends itself to mixed methods research, in a New Zealand European and Māori partnership, to conduct bicultural research. The authors use the Māori metaphor He awa whiria (braided river) to describe combining the strengths of two distinct worldviews into a “workable whole.” A framework brings together these two different paradigms as equals, incorporating both numerical and opinion-driven results. The authors illustrate this with a research example of creating a bicultural research framework, underpinned by mixed methods research philosophy.

Taxonomic notes on the New Zealand flora: lectotypes in Dryopteridaceae and Nephrolepidaceae

2018 ◽  
Vol 57 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Patrick J. Brownsey ◽  
Leon R. Perrie ◽  
Ashley R. Field
Keyword(s):  
New Zealand ◽  
New Zealand Flora
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