Potential for using Vasconcellea parviflora as a bridging species in intergeneric hybridisation between V. pubescens and Carica papaya

2009 ◽  
Vol 57 (7) ◽  
pp. 592 ◽  
Author(s):  
C. M. O'Brien ◽  
R. A. Drew

There have been numerous attempts to transfer Papaya ringspot virus type P (PRSV-P) resistance from wild Vasconcellea relatives to Carica papaya L. Success has been limited by the high degree of genetic divergence and thus incompatibility between Vasconcellea spp. and C. papaya. In the present work, intergeneric hybrid populations of C. papaya × V. pubescens Lenne et Koch, C. papaya × V. parviflora A. DC. and interspecific populations of V. pubescens × V. parviflora were produced and evaluated for morphological characteristics and PRSV-P resistance. F2 and F3 populations were produced from the V. pubescens × V. parviflora F1 and PRSV-P resistant individuals with homozygous genotypes (RR) were selected with the aid of a co-dominant CAPS marker and backcrossed to V. parviflora or outcrossed to C. papaya. Leaf vein number (seven) from V. pubescens, flower colour (pink) from V. parviflora and petiole colour (red–green) and plant size from C. papaya were transferred as dominant traits in interspecific and intergeneric crosses. Other traits, e.g. flower shape and fruit size, were intermediate in hybrids. When the wild species or their hybrids were outcrossed to C. papaya, inheritance patterns did not always follow Mendelian ratios, suggesting abnormal pairing of chromosomes or preferential elimination of the Vasconcellea genes. For example, in crosses between C. papaya × V. pubescens, all flowers were female and in crosses between C. papaya × V. parviflora, the cream flower colour of C. papaya was dominant over the pink colour of V. parviflora. However, the PRSV-P resistance gene from V. pubescens was successfully backcrossed into V. parviflora from F2 hybrids, and in the F1 hybrids between C. papaya and V. parviflora, 45% pollen fertility was obtained thus V. parviflora is proposed as a potential bridging species between C. papaya and V. pubescens.

2009 ◽  
pp. 102-112
Author(s):  
Florence Lasalita-Zapico ◽  
Violeta Villegas

Morphological analysis was undertaken to assess the degree of genetic relatedness and to characterize selected papaya (Carica papaya L.) inbreds and hybrids. Transmission of phenotypic traits from inbred parents to hybrid progeny followed the Mendelian pattern (complete dominance). The clustering mechanism separated the papaya genotypes into two groups. It was also revealed that some inbred lines presumably of very diverse origins exhibited similar morphological characteristics, raising the possibility that they have phylogenetic affinities and/or common origins. Screening for morphological traits with a high degree of polymorphism and with invariable expressions of the phenotypes would help in the identification of markers for hybrid identification and also in the accurate estimation of genetic relatedness among these hybrids and their parents.


2014 ◽  
Vol 20 (3-4) ◽  
Author(s):  
R. Sóspataki ◽  
M. Józsa ◽  
G. Simon

Lycium barbarum is known exclusively as an ornamental plant in Hungary, and is planted so, as popular belief deem it a toxic plant. The plant’s fruit receives great respect in countries abroad, thus, due to its favourable content values the Lycium has achieved the title of ‘biological gold mine’. The last couple of years has seen the import of Lycium shrubs and its corresponding products, dried goods or in the form of various processed products which have been marketed and sold at extreme prices (under name of Goji, Wolfberry, Lifeberry). Our goal was to examine the similarities of the content values of wolfberry found in Hungary and those cultivated abroad. Along with Miklós Józsa the domestic Lycium population was surveyed based on foreign examples, between the years 2009 and 2011. Those defined sweet and largefruited were selected for further investigations to be set into a clone repository. This clone repository– which contains 67 different clones from a number of regions of Hungary – was established in the nursery of Dr. Miklós Józsa, located in the city of Szombathely. The phenological and morphological characteristics and the fruit ripening and quality indicators of plants in the clone repository were investigated. Six ‘best’ clones – selected based on flavour, disease-resistance and vegetative characteristics (plant size, fruit set, yield and fruit size) were analysed based on their content values. The control plant was a cultivar imported by a delivery service, found also in growing. In addition to the results of the selection, the results of the content values of the six selected ‘best’ clones (total soluble sugar content, glucose-fructose ratio, carotene content, FRAP value) is documented in this paper. Further, based on the resulting information the possibilities of the fruit’s utilization are suggested. Significant differences were measured in the vegetative characteristics of the clones (plant size, fruit set, yield and fruit size) and of those ofits inner content values. The resulting properties are considerable in regards of the plant’s introduction into cultivation and also utilization. Based on growing characteristics, more favourable clones were selected than the foreign varieties already in growing. Those clones selected by us have had similar or better content values than foreign breeds. The investigation of these content values is still in progress.


2006 ◽  
Vol 46 (3) ◽  
pp. 413 ◽  
Author(s):  
R. A. Drew ◽  
S. V. Siar ◽  
C. M. O'Brien ◽  
P. M. Magdalita ◽  
A. G. C. Sajise

Papaya ringspot virus type P (PRSV-P) is a major threat to the papaya industry worldwide. F1 hybrids have been produced when Carica papaya L. female flowers have been pollinated with pollen of the PRSV-P resistant species Vasconcellea quercifolia. F1 plant production required embryo rescue 90 days post-pollination, and plantlet regeneration in vitro. Three hundred F1 hybrids were grown to maturity in the field and had morphological characteristics that were identical to 1 or both parents, were intermediate between those of the parents, or were greater than either parent. They had a sex ratio of 2 (male) : 49 (hermaphrodite) : 49(female). Eighteen plants (7 male and 11 hermaphrodite) produced some viable pollen. Pollen viability of these plants as measured by germination on agar medium varied between 1.1 and 6.1% (mean of 3.37 ± 0.18) compared with >90% for C. papaya. Cytogenetic studies showed limited homology between C. papaya and V. quercifolia genomes. The intergeneric hybrids varied in their reaction to strains of PRSV-P in Australia and the Philippines. Of the hybrids, 75% were resistant to PRSV-P while 25% produced virus symptoms.


2019 ◽  
Vol 37 ◽  
Author(s):  
N. KANDEMIR ◽  
G. KHAN ◽  
A. ÇELIK

ABSTRACT: Iris is the largest genus of family Iridaceae having 50 species with 24 endemic to Turkey. Here in this study we compared morphological, anatomical and ecological characters of two varieties of Iris unguicularis subsp. carica (Iris unguicularis subsp. carica var. carica and Iris unguicularis subsp. carica var. syriaca) from Turkey. We reported some distinctive morphological characteristics between both of the varieties like plant size, root length and width, leaf length and width, perianth tube, falls, standard fruit and bract length, falls and standards width and structure, flower colour which are of important taxonomic values. We further investigated that both the varieties are found in different localities and the differences in morphological properties are specific to ecological conditions. Soil analysis of both varieties reveal that CO3, Ca, Mg, Fe and Mn values are more effective than the other soil factors in the distributions of these varieties.


1983 ◽  
Vol 23 (120) ◽  
pp. 106 ◽  
Author(s):  
WB McGlasson ◽  
JB Sumeghy ◽  
LL Morris ◽  
RL McBride ◽  
DJ Best ◽  
...  

F1 hybrids of the nor non-ripening mutant tomato in different genotypic backgrounds were evaluated between 1978-81. The nor gene in the heterozygous condition delayed the start of ripening by a few days, increased the interval between breaker and the table ripe stage to 10 d at 21�C compared with 6 d for fixed cultivars and increased the storage life of ripe fruit at 21�C by about 50%. The retention of firmness by ripe fruit of the hybrids was affected by parental genotype. Fruit of some hybrids was firmer or as firm as fruit of the hard commercial Flora-Dade but others were much softer. A major problem with fruit of F1 nor hybrids was poor colour development. The fruit ripened to an orange-red colour, and strains with green shoulders developed an unattractive yellow on the shoulders. This deficiency was most pronounced in fruit picked before the appearance of red colour. The best hybrid found in this study was 75T10-1 x nor backcross 4 or 5 Heinz 1350. Fruit size, soluble solids, pH, titratable acidity, total ascorbic acid levels and acceptability except external colour of table ripe fruit appeared to be influenced by the parental genotype rather than by the nor gene. Since there were marked differences between hybrids with different genotypic backgrounds, it should be possible to breed nor hybrids with improved colour and firmness.


Author(s):  
Michaela Benková ◽  
Iveta Čičová ◽  
Daniela Benedikova ◽  
Lubomir Mendel ◽  
Miroslav Glasa

Abstract The work is focused on the evaluation of variability of morphological and pomological characteristics of several old sweet cherries (Prunus avium L.) that were found in different Slovak regions. The experimental work has been performed during two years, 2014 and 2015. The following characteristics according to the descriptor list of subgenus Cerasus were evaluated - period of flowering and ripening, morphological characteristics of the flowers, fruit size, fruit weight, and quality parameters. The results showed high variability of evaluated accessions. From the 13 surveyed localities, the most valuable accessions were found in the locality Hornį Streda - places Čachtice, Krakovany, Nitra, and Brdárka. During the collecting expeditions, 170 accessions of sweet cherry, with fruit of the different quality were found. The most interesting accessions were grafted onto rootstocks with different intensity of growth (Prunus avium L., Prunus mahaleb L., and ‘Gisela5’). Some of the selected cherry accessions can be used for commercial growing after tests, while some of them can be used only for collection of genetic resources and as potential genitors in breeding programmes.


2013 ◽  
Vol 5 (1) ◽  
pp. 90-97 ◽  
Author(s):  
Srikrishna LATHA ◽  
Deepak SHARMA ◽  
Gulzar S. SANGHERA

The nature and magnitude of heterosis and combining ability was studied in 18 F1 hybrids involving three CMS lines and six testers using line × tester analysis. The analysis of variance for combining ability of all the traits showed that variances due to treatments, parents, hybrids were highly significant. The line ‘CRMS 32A’ and testers viz. ‘Super rice-8’, ‘R 1099-2569-1-1’ and ‘Jitpiti’ were identified as good general combiners. The significant differences between lines x testers interaction indicates that SCA attributed heavily in the expression of these traits and demonstrates the importance of dominance or non additive variances for all the traits. The hybrid ‘CRMS 32A’/‘R 1099-2569-1-1’ and ‘APMS 6A’/‘Super rice-8’ were promising for grain yield. The magnitude of relative heterosis, heterobeltiosis and standard heterosis were also estimated for different characters. A high degree of relative heterosis was observed for grain yield (20.45- 82.37%) in the hybrids viz., ‘CRMS 32A’/‘Super rice-8’, ‘APMS 6A’/‘Super rice-8’, ‘APMS 6A’/‘Jitpiti’ and ‘CRMS 32A’/‘R 1099-2569-1-1’. While, a higher degree of: heterobeltiosis (13.60 -68.37%) was observed for grain yield in the hybrids viz., ‘CRMS 32A’/‘Super rice-8’, ‘CRMS 32A’/‘R 1099-2569-1-1’, ‘APMS 6A’/’Super rice-8’ and ‘APMS 6A’/’Jitpiti’. A high degree of standard heterosis was observed for grain yield in the hybrid ‘CRMS 32A’/‘R 1099-2569-1-1’. The hybrid ‘CRMS 32A’/ ‘R 1099-2569-1-1’ recorded a high degree of relative heterosis (62.01%), heterobeltiosis (57.35%) and standard heterosis (15.05 and 25.51% over check hybrids, ‘Mahamaya’ and ‘Indirasona’, respectively) that can be tested on yield trials for its further testing over locations.


2021 ◽  
Author(s):  
Carmen Julia Figueredo-Urbina ◽  
Gonzalo D. Álvarez-Ríos ◽  
Mario Adolfo García-Montes ◽  
Pablo Octavio-Aguilar

Abstract Background: The agaves are plants of cultural importance that have been used by humans for about 10,000 years for about 40 specific uses, of which the most culturally and economically important are fermented (Pulque) and distilled beverages (Mescal). Pulque continues to be produced in nearly all of Mexico, and the agaves used for this propose have shown domestication syndrome and selected morphological characteristics, such as larger plant size (since size is positively related to sap production) and less dentition of the leaves.Methods: In this study, we carry out an ethnobotanical, morphological and genetic analysis of the traditional varieties of pulque agave used in the production of aguamiel (agave sap) and pulque in two localities of the state of Hidalgo. We did semi-structured interviews and tours with crop owners, tlachiqueros (sap harvesters), and people related to the production system. We analyzed the morphology of 111 plants, and we studied the diversity and genetic structure of 130 individuals using 16 nuclear microsatellites.Results: We found wild, tolerated, transplanted, and cultivated varieties of agave. Asexual reproduction was the main method of propagation. We recorded 19 traditional varieties of pulque agave, 12 of them in production during the study, which correspond to the species Agave americana, A. salmiana y A. mapisaga with five intraspecific entities. The varieties were grouped according to a management gradient; the wild varieties were the smallest, with more lateral teeth and a larger terminal spine. The cultivated varieties clearly exhibited domestication syndrome, with larger plants and smaller dentition. The expected heterozygosity (He) of the varieties ranged between 0.204 to 0.721. Bayesian clustering suggested the existence of three genetic groups (K = 3), both at the level of traditional varieties of pulque agaves and for management categories, a result that matches multivariate clustering.Conclusions: Pulque producers in the studied localities, maintain high agrobiodiversity. The cultivated varieties exhibit domestication syndrome, as has been reported for other species of the genus with the same selection purposes. Our results support the hypothesis of a decrease in genetic diversity in crops compared to wild agaves, which seems to be due to vegetative propagation, among other factors.


2020 ◽  
pp. 84-87
Author(s):  
S. V. Koroleva ◽  
N. B. Shulyak

Relevance. The most complex and common physiological disease on the cabbage is a tip burn, associated with impaired Ca2 + transport inside the plant. Of all the ways to control this physiological disease, the most effective is the cultivation of tolerant hybrids. Material and methods.The aim of the research was to evaluate the mid-late and late ripening F1 hybrids of white cabbage for a tip burn and to determine the degree of hybrids responsiveness to calcium deficiency depending on weather conditions. The studies were carried out at breeding and experimental plot of the vegetable growing department at All-Russian Rice Research Institute in 2017-2019 on drip irrigation. Material - hybrid combinations of ARRRI breeding.Results. The manifestation of a physiological disturbance called “burn of the top of the inner leaves of the cabbage head” or “tip burn” on individual hybrids was observed during all years of cultivation, but to a different degree; The greatest damage to the heads was recorded in the years (2017 and 2019) with high productivity, which is due to the intensive assimilation ability against the background of warm sunny weather. In 2019, the manifestation of burns was noted in 25% of hybrids, which allowed them to be divided into 4 groups, depending on the degree of damage and the spread of the disease.It was established that hybrids with overmature standing for 2.5-3 weeks and also hybrids that were promptly removed at the time of mass technical ripeness had a high degree of damage. The lines that are most often found in hybrid combinations with signs of burns (269-824, Agr1321, 269Jas13, Byum112, Yas25, etc.) were identified, but it is rather difficult to assume the nature of inheritance, and, therefore, to develop the principle of pair selection when developing relatively stable hybrids also not yet possible.


Plants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 328 ◽  
Author(s):  
Ana V. González-de-Peredo ◽  
Mercedes Vázquez-Espinosa ◽  
Estrella Espada-Bellido ◽  
Marta Ferreiro-González ◽  
Antonio Amores-Arrocha ◽  
...  

Myrtus communis L. is an evergreen shrub that produces berries with a high content in antioxidant compounds. Since these compounds have demonstrated a positive effect on human health, the interest on berries and their usages has increased. However, environmental conditions may affect the productivity of these species and consequently the quality of wild myrtle. Ecotypes from diverse geographical origins may result in significant variations in terms of bioactive compounds content as well as in chemical traits. For this reason, in this work ecotypes from two different localizations have been studied to determine if their differences in morphological and anthocyanins traits can be attributed to their origin and the environmental characteristics of these locations. For this, chemometric analyses such as Hierarchical Cluster Analysis and Principal Component Analysis, were employed. The results showed differences between the ecotypes depending on their location. In particular, myrtle berries from maritime zones present greater fruit size and amount of bioactive compounds, which means an improvement in the quality of the final product based on this raw material. It can be concluded that both morphological and anthocyanins traits are related to the location of the ecotype and allow selecting the best ecotype for the required applications.


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