scholarly journals Effects of Lys-ß-urogastrone in vivo

1988 ◽  
Vol 41 (4) ◽  
pp. 463 ◽  
Author(s):  
Anne J Campbell ◽  
S S Adams ◽  
Mary W Davey ◽  
DA Titchen
Keyword(s):  
Body Weight ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Neonatal Mice ◽  
Epidermal Growth

Lys-�-urogastrone, an analogue of human {1-urogastrone with an additional N-terminal lysine, was shown to have similar effects in mice and sheep to mouse epidermal growth factor (mEGF). Lys-{1- urogastrone in doses of 0'18-3 �24 "g g-I body weight caused both precocious separation of eyelids and eruption of incisors in neonatal mice.

Effect of epidermal growth factor on glucosamine-6-phosphate synthetase activity in the colon of neonatal mice

1985 ◽  
Vol 105 (2) ◽  
pp. 197-200 ◽  
Author(s):  
M. Hiramatsu ◽  
M. Kashimata ◽  
N. Minami ◽  
N. Minami ◽  
M. Kumegawa
Keyword(s):  
Body Weight ◽  
Enzyme Activity ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Colonic Mucosa ◽  
Single Injection ◽  
Synthetase Activity ◽  
Apparent Effect ◽  
Neonatal Mice ◽  
Epidermal Growth

ABSTRACT Epidermal growth factor (EGF), administered subcutaneously to neonatal mice at daily doses of 1, 2 and 4 μg/g body weight for 3 days, significantly increased glucosamine-6-phosphate synthetase activity in the colon. A single injection of EGF at doses of 2 and 4 μg/g body weight also significantly increased enzyme activity. When administered to mature mice, EGF (4 μg/g body weight for 3 days) had no apparent effect on the enzyme activity. From these results, we suggest that EGF acts as a trophic factor for the maturation of the colonic mucosa of neonatal mice. J. Endocr. (1985) 105, 197–200

scholarly journals Epidermal growth factor receptor kinase translocation and activation in vivo.

1986 ◽  
Vol 261 (18) ◽  
pp. 8473-8480
Author(s):  
D G Kay ◽  
W H Lai ◽  
M Uchihashi ◽  
M N Khan ◽  
B I Posner ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Factor Receptor ◽  
Receptor Kinase ◽  
Epidermal Growth

In vivo fluorescence imaging for cancer diagnosis using receptor-targeted epidermal growth factor-based nanoprobe

Biomaterials ◽  
2013 ◽  
Vol 34 (36) ◽  
pp. 9149-9159 ◽  
Author(s):  
Ju Hee Ryu ◽  
Miyoung Shin ◽  
Sun Ah Kim ◽  
Sangmin Lee ◽  
Hyunjoon Kim ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Fluorescence Imaging ◽  
Cancer Diagnosis ◽  
In Vivo Fluorescence ◽  
Epidermal Growth ◽  
In Vivo Fluorescence Imaging

Modulation of epidermal growth factor receptor proto-oncogene transcription by a promoter site sensitive to S1 nuclease

1988 ◽  
Vol 8 (10) ◽  
pp. 4174-4184
Author(s):  
A C Johnson ◽  
Y Jinno ◽  
G T Merlino
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Egf Receptor ◽  
Receptor Gene ◽  
Gene Promoter ◽  
Direct Repeat ◽  
S1 Nuclease ◽  
Epidermal Growth ◽  
Specific Factors

The epidermal growth factor (EGF) receptor is the functional target of the mitogen EGF and the cellular homolog of the avian erythroblastosis virus erbB oncogene product. Regulation of expression of the proto-oncogene encoding the EGF receptor can be elucidated by studying the structure and function of the gene promoter outside the confines of the cell. Previously, we reported the isolation of the human EGF receptor gene promoter. The promoter is highly GC rich, contains no TATA or CAAT box, and has multiple transcription start sites. An S1 nuclease-sensitive site has now been found 80 to 110 base pairs (bp) upstream from the major in vivo transcription initiation site. Two sets of direct repeat sequences were found in this area; both conform to the motif TCCTCCTCC. When deletion mutations were made in this region of the promoter by using either Bal 31 exonuclease or S1 nuclease, we found that in vivo activity dropped three- to fivefold, on the basis of transient-transfection analysis. Examination of nuclear protein binding to normal and mutated promoter DNAs by gel retardation analysis and DNase I footprinting revealed that two specific factors bind to the direct repeat region but cannot bind to the S1 nuclease-mutated promoter. One of the specific factors is the transcription factor Sp1. The results suggest that these nuclear trans-acting factors interact with the S1 nuclease-sensitive region of the EGF receptor gene promoter and either directly or indirectly stimulate transcription.

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