scholarly journals Satellite DNA Sequences in the Red Kangaroo (Macropus rufus)

1982 ◽  
Vol 35 (3) ◽  
pp. 313 ◽  
Author(s):  
A Elizur ◽  
E S Dennis ◽  
WJ Peacock
Keyword(s):  
Dna Sequences ◽  
Satellite Dna ◽  
Complex Pattern ◽  
Equilibrium Density ◽  
Density Gradients ◽  
Hoechst 33258 ◽  
Main Band ◽  
Red Kangaroo ◽  
Macropus Rufus

There is a complex pattern of satellite DNA sequences in M. rufus which are revealed by addition of Ag+ or dye (Hoechst 33258) to the DNA in CS2S04 or CsCI equilibrium density gradients. Six satellite DNA fractions have been isolated; these have buoyant densities in neutral CsCI of 1� 692, 1� 704, 1� 705, 1� 707 (two), 1� 710 and 1� 712 gjml compared with 1� 696 g/ml for the main band DNA. Each satellite accounts for 1-3 % of the DNA of the genome.

scholarly journals Divergence, differential methylation and interspersion of melon satellite DNA sequences

1981 ◽  
Vol 195 (3) ◽  
pp. 723-734 ◽  
Author(s):  
R Shmookler Reis ◽  
J N Timmis ◽  
J Ingle
Keyword(s):  
Dna Sequences ◽  
Satellite Dna ◽  
Dna Analysis ◽  
Buoyant Density ◽  
Sequence Divergence ◽  
Main Band ◽  
Repeat Array ◽  
Tandem Repeat Sequences ◽  
Tandem Arrays

Melon (Cucumis melo) satellite DNA consists of two components, Q and S, each with a buoyant density in CsCl of 1.707 g/ml, but differing by 9 degrees C in “melting” temperature. These physical properties appear to be in contradiction, since both depend on G + C content. In order to resolve this anomaly, base compositions were directly determined for isolated fractions. the low-“melting” component S contains 41.8% G + C, with 6% of C present as 5-methylcytosine, whereas Q DNA contains 54% G + C, with 41% of C methylated. Analyses of restriction site loss agreed well with the direct determinations of methylation and divergence, and indicated some clustering of methylated sites in Q DNA. Analysis of restricted main-band DNA by hydridization with RNA complementary to Q satellite DNA (“Southern transfer”) showed satellite Q tandem arrays interspersed in DNA of main-band density. Sequence divergence and extent of methylation did not appear to depend on whether a repeat array was present as satellite or interspersed in main-band DNA. Hydridization in situ indicated considerable heterogeneity in the genomic proportion of the Q-DNA sequences in melon fruit nuclei, implying over- and under-representation consistent with extensive unequal recombination in satellite Q tandem arrays. The cucumber, Cucumis sativus, contains less than 8% as much Q-homologous DNA per genome as the melon, suggesting rapid evolutionary gain or loss of these tandem repeat sequences.

Diagnosis and treatment of mesenteric volvulus in a red kangaroo (Macropus rufus)

2014 ◽  
Vol 244 (7) ◽  
pp. 844-850 ◽  
Author(s):  
S. Emmanuelle Knafo ◽  
Alana J. Rosenblatt ◽  
James K. Morrisey ◽  
James A. Flanders ◽  
Margret S. Thompson ◽  
...  
Keyword(s):  
Diagnosis And Treatment ◽  
Red Kangaroo ◽  
Macropus Rufus

scholarly journals Transcription termination and processing of transcripts from tRNA-related Xenopus satellite DNA sequences

1987 ◽  
Vol 164 (2) ◽  
pp. 287-293 ◽  
Author(s):  
Wolfgang MEYERHOF ◽  
Burghardt WITTIG ◽  
Beatrix TAPPESER ◽  
Walter KNOCHEL
Keyword(s):  
Dna Sequences ◽  
Satellite Dna ◽  
Transcription Termination

Dissociation of minor satellite from the centromere in mouse

1994 ◽  
Vol 107 (11) ◽  
pp. 3091-3095
Author(s):  
B.K. Vig ◽  
D. Latour ◽  
J. Frankovich
Keyword(s):  
Satellite Dna ◽  
Hoechst 33258 ◽  
Major Satellite ◽  
Primary Constriction ◽  
Minor Satellite ◽  
Antibody Reaction

The minor satellite DNA of mouse is believed to constitute the centromere. We report that centromeres of some chromosomes in the Cl1D cells of mouse are not associated with this DNA even though the latter is present on these chromosomes. The satellite DNA was detected distally from the centromere and could not be mistaken as a component of the centromere. We also report that the site of the primary constriction may not always coincide with the site of the anti-kinetochore antibody reaction. Whereas the regions containing the major satellite decondense upon treatment with bisbenzimidazole (Hoechst 33258), the sites carrying minor satellite resist decondensing.

Epigenetic control of mammalian centromere protein binding: does DNA methylation have a role?

1996 ◽  
Vol 109 (9) ◽  
pp. 2199-2206
Author(s):  
A.R. Mitchell ◽  
P. Jeppesen ◽  
L. Nicol ◽  
H. Morrison ◽  
D. Kipling
Keyword(s):  
Dna Sequences ◽  
Satellite Dna ◽  
Inbred Mouse ◽  
Inbred Mouse Strain ◽  
Chromosome 1 ◽  
Satellite Sequences ◽  
E Protein ◽  
Minor Satellite ◽  
Internal Block ◽  
Terminal Block

Chromosome 1 of the inbred mouse strain DBA/2 has a polymorphism associated with the minor satellite DNA at its centromere. The more terminal block of satellite DNA sequences on this chromosome acts as the centromere as shown by the binding of CREST ACA serum, anti-CENP-B and anti-CENP-E polyclonal sera. Demethylation of the minor satellite DNA sequences accomplished by growing cells in the presence of the drug 5-aza-2′-deoxycytidine results in a redistribution of the CENP-B protein. This protein now binds to an enlarged area on the more terminal block and in addition it now binds to the more internal block of minor satellite DNA sequences on chromosome 1. The binding of the CENP-E protein does not appear to be affected by demethylation of the minor satellite sequences. We present a model to explain these observations. This model may also indicate the mechanism by which the CENP-B protein recognises specific sites within the arrays of minor satellite DNA on mouse chromosomes.

Chromatin organization in the homogeneously staining regions of a methotrexate-resistant mouse cell line: interspersion of inactive and active chromatin domains distinguished by acetylation of histone H4

1996 ◽  
Vol 109 (9) ◽  
pp. 2221-2228 ◽  
Author(s):  
L. Nicol ◽  
P. Jeppesen
Keyword(s):  
Cell Line ◽  
Dna Sequences ◽  
Satellite Dna ◽  
Mouse Cell ◽  
Histone H4 ◽  
Active Chromatin ◽  
Chromatin Domains ◽  
Resistant Mouse ◽  
Major Satellite ◽  
Drosophila Heterochromatin

We have analyzed the organization of the homogeneously staining regions (HSRs) in chromosomes from a methotrexate-resistant mouse melanoma cell line. Fluorescence in situ hybridization techniques were used to localize satellite DNA sequences and the amplified copies of the dihydrofolate reductase (DHFR) gene that confer drug-resistance, in combination with immunofluorescence using antibody probes to differentiate chromatin structure. We show that the major DNA species contained in the HSRs is mouse major satellite, confirming previous reports, and that this is interspersed with DHFR DNA in an alternating tandem array that can be resolved at the cytological level. Mouse minor satellite DNA, which is normally located at centromeres, is also distributed along the HSRs, but does not appear to interfere with centromere function. The blocks of major satellite DNA are coincident with chromatin domains that are labelled by an autoantibody that recognizes a mammalian homologue of Drosophila heterochromatin-associated protein 1, shown previously to be confined to centric heterochromatin in mouse. An antiserum that specifically recognizes acetylated histone H4, a marker for active chromatin, fails to bind to the satellite DNA domains, but labels the intervening segments containing DHFR DNA. We can find no evidence for the spreading of the inactive chromatin domains into adjacent active chromatin, even after extended passaging of cells in the absence of methotrexate selection.

Bilateral radial and ulnar fractures in a red kangaroo (Macropus rufus)

2019 ◽  
Vol 255 (8) ◽  
pp. 942-948 ◽  
Author(s):  
Katherine L. Ballor ◽  
Krista M. Gazzola ◽  
Karen L. Perry
Keyword(s):  
Red Kangaroo ◽  
Macropus Rufus
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