scholarly journals Changes in the Concentration of Gonadotrophic and Steroidal Hormones in the Antral Fluid of Ovarian Follicles Throughout the Oestrous Cycle of the Sheep

1981 ◽  
Vol 34 (1) ◽  
pp. 67 ◽  
Author(s):  
KP McNatty ◽  
M Gibb ◽  
C Dobson ◽  
DC Thurley ◽  
JK Findlay
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Ovarian Follicles ◽  
Oestrous Cycle ◽  
Steroid Secretion ◽  
Steroidal Hormones ◽  
Follicle Size ◽  
The Individual ◽  
Secretion Rates ◽  
Venous Plasma

The concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, progesterone, androstenedione and oestradiol were determined in the antral fluid of ovarian follicles > 1 mm in diameter as well as in ovarian venous or peripheral venous plasma, or both, from at least four different animals on each day throughout the oestrous cycle of the sheep. The individual steroid hormones in antral fluid were examined in relation to the steroid-secretion rates in ovarian venous plasma, follicle size and the hormone levels in jugular venous plasma.

CONCENTRATION OF OESTRADIOL, PROGESTERONE, LUTEINIZING HORMONE AND FOLLICLE-STIMULATING HORMONE IN THE JUGULAR VENOUS PLASMA OF EWES DURING THE OESTROUS CYCLE

1977 ◽  
Vol 73 (2) ◽  
pp. 247-255 ◽  
Author(s):  
H. C. PANT ◽  
C. R. N. HOPKINSON ◽  
R. J. FITZPATRICK
Keyword(s):  
Luteinizing Hormone ◽  
Luteal Phase ◽  
Follicle Stimulating Hormone ◽  
Oestrous Cycle ◽  
Phase 3 ◽  
Ovarian Steroids ◽  
The Mean ◽  
Two Peaks ◽  
Distinct Increase ◽  
Venous Plasma

SUMMARY Changes in the concentrations of ovarian steroids and pituitary gonadotrophins were measured by radioimmunoassay in the jugular plasma of six Clun Forest ewes throughout the oestrous cycle. The concentration of oestradiol began to rise 12–14 h before the onset of oestrus from values of 11·2 ± 0·36 (s.e.m.) pg/ml during the luteal phase to 21·1 ± 2·01 pg/ml at −8 to 0 h (oestrus). There was no distinct increase during the luteal phase. Circulating progesterone varied in a cyclic manner with the highest values at the mid-luteal phase (3·70 ± 0·28 ng/ml; n = 28). In five out of six ewes the concentration was still quite high (1·86 ± 0·43 ng/ml) at 35 h before the onset of oestrus. The concentration declined rapidly thereafter, reaching minimum values about 12 h before oestrus coincident with the increase in oestradiol concentration. Plasma LH increased from very low values of 2·59 ± 0·09 ng/ml during the luteal phase to 75·3 ± 7·4 ng/ml about 9 h after the onset of oestrus. Two peaks of plasma FSH concentration were detected after the onset of oestrus. The first peak (171·0± 35·5 ng/ml) coincided with the LH peak and the second (133·0 ± 10·7 ng/ml) occurred about 24 h later at a time when LH values were low. The mean FSH concentration at other times during the cycle was 61·9 ± 2·8 ng/ml.

THE LEVELS OF FSH AND LH IN THE PITUITARY OF THE EWE IN RELATION TO FOLLICULAR GROWTH AND OVULATION

1962 ◽  
Vol 24 (2) ◽  
pp. 143-151 ◽  
Author(s):  
H. A. ROBERTSON ◽  
J. S. M. HUTCHINSON
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Ovarian Follicles ◽  
Anterior Lobe ◽  
The State ◽  
Oestrous Cycle ◽  
Follicular Growth ◽  
Ovulatory Period ◽  
Marked Drop ◽  
Stimulating Hormone

SUMMARY The levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) have been determined in the anterior lobe of the pituitary of ewes killed at precise stages of the oestrous cycle, during anoestrus, pregnancy, lactation, and in virgin ewes. A marked drop in the content of both FSH and LH has been demonstrated during the ovulatory period of the cycle. The values obtained are discussed in relation to the state of the ovarian follicles.

OVARIAN SECRETION RATES OF OESTROGENS, ANDROGENS AND PROGESTERONE IN NORMAL WOMEN AND IN WOMEN WITH PERSISTENT OVARIAN FOLLICLES

1974 ◽  
Vol 77 (3) ◽  
pp. 575-587 ◽  
Author(s):  
F. H. de Jong ◽  
D. T. Baird ◽  
H. J. van der Molen
Keyword(s):  
Menstrual Cycle ◽  
Production Rate ◽  
Follicular Fluid ◽  
Ovarian Follicles ◽  
Dehydroepiandrosterone Sulphate ◽  
Peripheral Plasma ◽  
Normal Women ◽  
Quantitative Contribution ◽  
Secretion Rates ◽  
Venous Plasma

ABSTRACT The concentrations of oestradiol-17β, oestrone, androstenedione, testosterone, dehydroepiandrosterone, dehydroepiandrosterone sulphate and progesterone were measured in ovarian venous plasma from one or both ovaries in 4 normal women during different stages of the menstrual cycle and in 4 women with persistent ovarian follicles. In addition the steroid concentrations in peripheral plasma and follicular fluid were estimated. All steroids mentioned, with the exception of dehydroepiandrosterone sulphate, were secreted by the ovaries. The concentrations of oestradiol-17β, oestrone, androstenedione and progesterone were higher in the venous plasma from the ovary containing the developing follicle or corpus luteum than in venous plasma from the contralateral ovary. There was a good correlation between ovarian secretion of the oestrogenic steroids and androstenedione. Finally, the quantitative contribution of the ovarian secretion to the blood production rates of the androgens and progesterone was calculated. The only contributions exceeding 20 % of the blood production rate were those of progesterone and androstenedione during the second half of the cycle.

LUTEINIZING HORMONE AND LUTEINIZING HORMONE RELEASING HORMONE-LIKE IMMUNOREACTIVITY IN THE JUGULAR VENOUS BLOOD OF SHEEP AT VARIOUS STAGES OF THE OESTROUS CYCLE

1976 ◽  
Vol 68 (3) ◽  
pp. 409-417 ◽  
Author(s):  
J. P. FOSTER ◽  
S. L. JEFFCOATE ◽  
D. B. CRIGHTON ◽  
D. T. HOLLAND
Keyword(s):  
Luteinizing Hormone ◽  
Breeding Season ◽  
Venous Blood ◽  
Oestrous Cycle ◽  
Maximal Level ◽  
Blood Samples ◽  
Luteinizing Hormone Releasing Hormone ◽  
Two Samples ◽  
Lh Rh ◽  
Venous Plasma

SUMMARY Luteinizing hormone and LH-RH-like immunoreactivity were measured in the jugular venous plasma of Clun Forest ewes at various stages of the oestrous cycle. Blood samples were collected through jugular venous cannulae every 2 h for at least 20 days from three ewes during the breeding season. The ewes were checked twice daily for oestrus using a vasectomized ram. Plasma LH peaks of apparent height 112–192 ng NIH-LH-S17 equivalents/ml were detected at oestrus with basal levels of 2–15 ng/ml during most of the remainder of the 17-day oestrous cycle. Peaks of LH-RH-like immunoreactivity occurred at various times of the cycle. The apparent maximal level of these peaks was 220 pg/ml compared with basal levels of < 10 pg/ml. Further ewes (two for each group) were sampled at 4 min intervals for 12 h, (1) from onset of oestrus, (2) 36–48 h after onset of oestrus or (3) on day 10 of the oestrous cycle. In the ewes sampled at oestrus, peaks of LH-RH-like immunoreactivity were detected before, during and after the preovulatory LH peak. Those detected after the LH peak were unassociated with any further increases in the plasma LH level. In the ewes sampled 36–48 h after onset of oestrus and on day 10 of the cycle, several peaks of LH-RH-like immunoreactivity unassociated with any increases in the LH level were detected. These peaks, and those detected at oestrus, had durations of only one or two samples, and in some cases reached levels of several ng/ml compared with basal levels of < 10 pg/ml. The significance of these results is discussed.

OESTROGEN SYNTHESIS IN RAT OVARIES IN VITRO: EFFECTS OF ENDOGENOUS AND EXOGENOUS GONADOTROPHINS

1972 ◽  
Vol 53 (3) ◽  
pp. 397-406 ◽  
Author(s):  
BRENDA ROBINSON ◽  
R. E. OAKEY
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Oestrous Cycle ◽  
The Other ◽  
Ovarian Vein ◽  
Hormone Activity ◽  
In Vitro Effects ◽  
Stimulating Hormone

SUMMARY The rate of synthesis of [14C]oestrone and [14C]oestradiol-17β from [14C]testosterone in vitro by ovaries from rats at different stages of the oestrous cycle was measured. The rate of [14C]oestrogen synthesis was highest in ovaries taken from rats in pro-oestrus and lowest in ovaries taken from rats early in the dioestrous phase of the cycle. Rates of synthesis in ovaries obtained from rats in the late dioestrous stage were intermediate between the rates of the other groups. The rates of [14C]oestrogen synthesis at these periods of the cycle paralleled the concentrations of oestrogens in ovarian vein plasma reported by other authors. Gonadotrophin preparations with either luteinizing hormone activity or both follicle-stimulating hormone and luteinizing hormone activities had no effect on [14C]oestrogen synthesis by rat ovaries in vitro at any of these stages of the oestrous cycle.

DEVELOPMENT AND APPLICATION OF HOMOLOGOUS RADIOIMMUNOASSAYS FOR PORCINE GONADOTROPHINS

1979 ◽  
Vol 81 (1) ◽  
pp. 1-10 ◽  
Author(s):  
J. L. VANDALEM ◽  
CH. BODART ◽  
G. PIRENS ◽  
J. CLOSSET ◽  
G. HENNEN
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Oestrous Cycle ◽  
Releasing Hormone ◽  
Porcine Serum ◽  
Lh Releasing Hormone ◽  
Stimulating Hormone

Antisera were raised against highly purified preparations of porcine luteinizing hormone (pLH) and follicle-stimulating hormone (pFSH). Highly specific and sensitive radioimmunoassay systems were developed. The antisera to LH and FSH were used at working dilutions of 1: 500 000 and 1: 200 000 respectively and the sensitivities of the assays were 0·1 ng LH/ml serum (3 × 10−12 mol/l) and 0·5 ng FSH/ml serum (1·5 × 10−11 mol/l). The LH and FSH preparations used as standards were 1·2 and 81 times as potent as NIH-LH-S15 and NIH-FSH-P1 respectively. Both assays were validated and adapted for the measurement of the gonadotrophin content of porcine serum. The concentrations of LH and FSH in blood were measured simultaneously in prepubertal sows throughout a 24 h period, in adult sows during the oestrous cycle and in both prepubertal and adult animals after treatment with LH releasing hormone.

EFFECT OF OVINE LUTEINIZING HORMONE ON THE SECRETION OF PROGESTERONE IN VITRO BY OVARIAN FOLLICLES FROM HYPOPHYSECTOMIZED RATS TREATED WITH FOLLICLE-STIMULATING HORMONE

1978 ◽  
Vol 77 (3) ◽  
pp. 419-420
Author(s):  
A. R. LABARBERA ◽  
MERO R. NOCENTI
Keyword(s):  
New York ◽  
Luteinizing Hormone ◽  
Corn Oil ◽  
Follicle Stimulating Hormone ◽  
Virgin Female ◽  
Ovarian Follicles ◽  
Female Rats ◽  
Hypophysectomized Rats ◽  
Stimulating Hormone

Department of Physiology, College of Physicians and Surgeons, Columbia University, New York 10032, U.S.A. (Received 14 November 1977) Ovarian follicles from oestrous, pro-oestrous and hypophysectomized rats have the capacity to secrete progestins in vitro and to respond to luteinizing hormone (LH) by increasing this secretion (Stoklosowa & Nalbandov, 1972; LaBarbera, Nocenti & Castellano, 1974; Lindner, Tsafriri, Lieberman, Zor, Koch, Bauminger & Barnea, 1974). Follicles from hypophysectomized rats, untreated or treated with follicle-stimulating hormone (FSH), were therefore studied in experiments in vitro to investigate further the gonadotrophic control of progesterone secretion. Mature virgin female rats (200–250 g) were hypophysectomized and, beginning on day 4 after the operation, received a total of 360 μg ovine FSH (NIH-FSH-S9)/100 g body weight, injected s.c. in corn oil as six divided doses, one every 12 h for 3 days. Untreated controls received corn oil only. On day 7 after hypophysectomy, the ovaries were excised and

Release of 3H2O from 1β,2β[3H]androstenedione by equine granulosa cells

1983 ◽  
Vol 104 (2) ◽  
pp. 227-232 ◽  
Author(s):  
E. V. YoungLai ◽  
J. F. Jarrell
Keyword(s):  
Luteinizing Hormone ◽  
Granulosa Cells ◽  
Negative Correlation ◽  
Follicular Fluid ◽  
Enzyme System ◽  
Follicle Stimulating Hormone ◽  
Significant Negative Correlation ◽  
Oestrous Cycle ◽  
Bicarbonate Buffer ◽  
Stimulating Hormone

Abstract. Granulosa cells were harvested from mares at various stages of the oestrous cycle and incubated in Krebs-Ringer bicarbonate buffer with 1β,2β[3H]androstenedione as substrate. The release of 3H2O expressed as cpm/h/mg protein varied from 44 000 to 768 000 in follicles from 7 mares. The release of 3H2O was not significantly altered by luteinizing hormone, follicle stimulating hormone or pregnant mare's serum gonadotrophin. There was a significant negative correlation between the release of 3H2O and the concentration of progesterone in the follicular fluid. Based on the assumption that the release of 3H2O represent total aromatization, these data suggest that the equine granulosa cells have a very active aromatizing enzyme system.

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