scholarly journals Haemoglobin Oxidation in Whole Blood Samples From Sheep in Relation to Glutathione Peroxidase Activity

1979 ◽  
Vol 32 (5) ◽  
pp. 451 ◽  
Author(s):  
JF Wilkins
Keyword(s):  
Glutathione Peroxidase ◽  
Oxidative Damage ◽  
Peroxidase Activity ◽  
Whole Blood ◽  
Alternative Assessment ◽  
Selenium Status ◽  
Glutathione Peroxidase Activity ◽  
Blood Samples ◽  
Whole Blood Samples

A high correlation was observed between HzOz-induced oxidation of haemoglobin and glutathione peroxidase activity in whole blood samples from sheep. A role of this enzyme in the prevention of oxidative damage to the erythrocyte and its contents has been previously demonstrated. The possibility of using haemoglobin oxidation in whole blood as an alternative assessment of glutathione peroxidase activity and hence of selenium status is proposed.

scholarly journals  Direct and indirect assessment of selenium status in sheep – a comparison

2012 ◽  
Vol 57 (No. 5) ◽  
pp. 219-223 ◽  
Author(s):  
L. Pavlata ◽  
L. Misurova ◽  
A. Pechova ◽  
T. Husakova ◽  
R. Dvorak
Keyword(s):  
Czech Republic ◽  
Glutathione Peroxidase ◽  
Whole Blood ◽  
Selenium Concentration ◽  
Photometric Method ◽  
Reference Ranges ◽  
Selenium Status ◽  
The Czech Republic ◽  
Blood Samples ◽  
Whole Blood Samples

The aim of this study was to determine the relationship between selenium concentration and activity of glutathione peroxidase (GSH-Px) in the whole blood of sheep and reference ranges for the activity of GSH-Px for evaluation of the selenium status of sheep in the Czech Republic. Selenium concentration and activity of glutathione peroxidase were determined in whole blood samples collected from 92 sheep in six herds. The GSH-Px activity in the samples was measured using the photometric method. Selenium in the sample was measured using the hydride technique atomic absorption spectrometry. Data on both parameters were processed using correlation and regression analysis in order to obtain reference values of GSH-Px for the indirect evaluation of the selenium status of sheep. The two variables showed a close and significant correlation (r = 0.95; P &lt; 0.01). The regression line, defined by the equation y = 7.5857x &ndash; 121.87 (linear) or y = &ndash;0.0167x<sup>2</sup> + 11.993x &ndash; 355.57 (polynomial), allowed us to determine the GSH-Px activity of 637, resp. 677 &mu;kat/l as equivalent to selenium concentration in whole blood of sheep 100 &mu;g/l. Mean selenium concentration and mean GSH-Px activity found in whole blood samples were 123.42 &plusmn; 57.84 &mu;g/l and 814.34 &plusmn; 463.64 &mu;kat/l, respectively. In this study, a close dependence of GSH-Px activity on the selenium concentration in the blood of sheep was found. Activity values of GSH-Px were determined for use in the diagnosis of sufficient selenium status in sheep in the Czech Republic (GSH-Px activity greater than 600 &mu;kat/l of whole blood). &nbsp;

Low Selenium Status in the Elderly Influences Thyroid Hormones

1995 ◽  
Vol 89 (6) ◽  
pp. 637-642 ◽  
Author(s):  
Oliviero Olivieri ◽  
Domenico Girelli ◽  
Margherita Azzini ◽  
Anna Maria Stanzial ◽  
Carla Russo ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Thyroid Hormones ◽  
Peroxidase Activity ◽  
Controlled Trial ◽  
The Elderly ◽  
Serum Selenium ◽  
Selenium Status ◽  
Glutathione Peroxidase Activity ◽  
Double Blind ◽  
Double Blind Placebo

1. Iodothyronine 5′-deiodinase, which is mainly responsible for peripheral triiodothyronine (T3) production, has recently been demonstrated to be a selenium-containing enzyme. In the elderly, reduced peripheral conversion of thyroxine (T4) to T3 and overt hypothyroidism are frequently observed. 2. We measured serum selenium and erythrocyte glutathione peroxidase (as indices of selenium status), thyroid hormones and thyroid-stimulating hormone in 109 healthy euthyroid subjects (52 women, 57 men), carefully selected to exclude abnormally low thyroid hormone levels induced by acute or chronic diseases or calorie restriction. The subjects were subdivided into three age groups. To avoid conditions of undernutrition or malnutrition, dietary records were obtained for a sample of 24 subjects, randomly selected and representative of the whole population for age and sex. 3. In order to properly assess the influence of selenium status on iodothyronine 5′-deiodinase type I activity, a double-blind placebo-controlled trial was also carried out on 36 elderly subjects, resident at a privately owned nursing home. 4. In the free-living population, a progressive reduction of the T3/T4 ratio (due to increased T4 levels) and of selenium and erythrocyte glutathione peroxidase activity was observed with advancing age. A highly significant linear correlation between T4, T3/T4 and selenium was observed in the population as a whole (for T4, R = −0.312, P < 0.002; for T3/T4 ratio, R = 0.32, P < 0.01) and in older subjects (for T4, R = −0.40, P < 0.05; for T3/T4 ratio, R = 0.54, P < 0.002). 5. The main result of the double-blind placebo-controlled trial was a significant improvement of selenium indices and a decrease in the T4 level in selenium-treated subjects; serum selenium, erythrocyte glutathione peroxidase activity and thyroid hormones did not change in placebo-treated subjects. 6. We concluded that selenium status influences thyroid hormones in the elderly, mainly modulating T4 levels.

Whole blood glutathione peroxidase activity in melanoma patients

1999 ◽  
Vol 144 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Marı́a Rosa Ros-Bullón ◽  
Paloma Sánchez-Pedreño ◽  
José Hilario Martı́nez-Liarte
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Whole Blood ◽  
Glutathione Peroxidase Activity ◽  
Melanoma Patients

scholarly journals Probucol Increases Striatal Glutathione Peroxidase Activity and Protects against 3-Nitropropionic Acid-Induced Pro-Oxidative Damage in Rats

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e67658 ◽  
Author(s):  
Dirleise Colle ◽  
Danúbia Bonfanti Santos ◽  
Eduardo Luiz Gasnhar Moreira ◽  
Juliana Montagna Hartwig ◽  
Alessandra Antunes dos Santos ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Oxidative Damage ◽  
Peroxidase Activity ◽  
Glutathione Peroxidase Activity ◽  
Nitropropionic Acid ◽  
3 Nitropropionic Acid

Methods compared for determining glutathione peroxidase activity in blood.

1987 ◽  
Vol 33 (4) ◽  
pp. 539-543 ◽  
Author(s):  
B Faraji ◽  
H K Kang ◽  
J L Valentine
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Reference Intervals ◽  
The Other ◽  
Specific Time ◽  
Glutathione Peroxidase Activity ◽  
Time Intervals ◽  
Indirect Methods ◽  
Blood Samples

Abstract We compared four methods for determination of glutathione peroxidase (EC 1.11.1.9) activity, using blood samples from 52 healthy volunteers. Two methods depended on direct assay of the amount of glutathione remaining at specific time intervals; the two indirect methods involved measuring the rate of disappearance of NADPH. We assessed the precision and reproducibility of each method. One of the indirect assays proved to be far superior to the other methods. Results of each of the methods were correlated with one another. We present the normal reference intervals for glutathione peroxidase activity for all four methods.

Sign in / Sign up

Export Citation Format

Share Document