scholarly journals Studies on Wheat Endosperm III. Galactose-Rich Polysaccharides

1973 ◽  
Vol 26 (4) ◽  
pp. 1005 ◽  
Author(s):  
D JMares ◽  
BA Stone
Keyword(s):  
Cell Walls ◽  
Aqueous Ethanol ◽  
Endosperm Cell ◽  
Wheat Endosperm ◽  
Nylon Mesh

Galactose-rich polysaccharides have been found in the finely divided material passing 75-JLm nylon-mesh sieves during the isolation of wheat endosperm cell walls in 70 % aqueous ethanol.

scholarly journals Isolation of wheat endosperm cell walls: Effects of non-endosperm flour components on structural analyses

2017 ◽  
Vol 74 ◽  
pp. 165-173 ◽  
Author(s):  
Ghanendra Gartaula ◽  
Sushil Dhital ◽  
Denise Pleming ◽  
Michael J. Gidley
Keyword(s):  
Cell Walls ◽  
Endosperm Cell ◽  
Wheat Endosperm

Effect of Dough Mixing on Wheat Endosperm Cell Walls

2013 ◽  
Vol 61 (10) ◽  
pp. 2522-2529 ◽  
Author(s):  
Geraldine A. Toole ◽  
Elisa Selvatico ◽  
Louise J. Salt ◽  
Gwénaëlle Le Gall ◽  
Ian J. Colquhoun ◽  
...  
Keyword(s):  
Cell Walls ◽  
Endosperm Cell ◽  
Wheat Endosperm ◽  
Dough Mixing

scholarly journals Studies on Wheat Endosperm I. Chemical Composition and Ultrastructure of the Cell Walls

1973 ◽  
Vol 26 (4) ◽  
pp. 793 ◽  
Author(s):  
D JMares ◽  
BA Stone
Keyword(s):  
Chemical Composition ◽  
Cell Walls ◽  
Endosperm Cell ◽  
Wheat Endosperm ◽  
Ethanol Medium

A method is described for the isolation of wheat endosperm cell walls free from non-endospermic cell walls in a 70 % ethanol medium which prevents the loss of watersoluble polymeric components.

Wheat endosperm cell walls: Spatial heterogeneity of polysaccharide structure and composition using micro-scale enzymatic fingerprinting and FT-IR microspectroscopy

2009 ◽  
Vol 50 (3) ◽  
pp. 312-317 ◽  
Author(s):  
Luc Saulnier ◽  
Paul Robert ◽  
Mathilde Grintchenko ◽  
Frédéric Jamme ◽  
Brigitte Bouchet ◽  
...  
Keyword(s):  
Spatial Heterogeneity ◽  
Cell Walls ◽  
Endosperm Cell ◽  
Wheat Endosperm ◽  
Micro Scale ◽  
Ft Ir ◽  
Ir Microspectroscopy

FTIR imaging of wheat endosperm cell walls in situ reveals compositional and architectural heterogeneity related to grain hardness.

Planta ◽  
2004 ◽  
Vol 220 (5) ◽  
pp. 667-677 ◽  
Author(s):  
C. Barron ◽  
M. L. Parker ◽  
E. N. C. Mills ◽  
X. Rouau ◽  
R. H. Wilson
Keyword(s):  
Cell Walls ◽  
Endosperm Cell ◽  
Grain Hardness ◽  
Ftir Imaging ◽  
Wheat Endosperm

Quantitative structural organisation model for wheat endosperm cell walls: Cellulose as an important constituent

2018 ◽  
Vol 196 ◽  
pp. 199-208 ◽  
Author(s):  
Ghanendra Gartaula ◽  
Sushil Dhital ◽  
Gabriele Netzel ◽  
Bernadine M. Flanagan ◽  
Gleb E. Yakubov ◽  
...  
Keyword(s):  
Cell Walls ◽  
Endosperm Cell ◽  
Wheat Endosperm ◽  
Structural Organisation ◽  
Important Constituent

scholarly journals Studies on Wheat Endosperm II. Properties of the Wall Components and Studies on Their Organization in the Wall

1973 ◽  
Vol 26 (4) ◽  
pp. 813 ◽  
Author(s):  
D JMares ◽  
BA Stone
Keyword(s):  
Ammonium Sulphate ◽  
Cell Walls ◽  
Endosperm Cell ◽  
Wheat Endosperm ◽  
Exclusion Chromatography ◽  
Ammonium Sulphate Fractionation ◽  
Wall Components

Water- and alkali-soluble arabinoxylans from isolated wheat endosperm cell walls were examined by ammonium sulphate fractionation and gel-exclusion chromatography.

scholarly journals Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNAi lines with suppression of genes responsible for backbone synthesis and decoration

2017 ◽  
Vol 15 (11) ◽  
pp. 1429-1438 ◽  
Author(s):  
Jackie Freeman ◽  
Jane L. Ward ◽  
Ondrej Kosik ◽  
Alison Lovegrove ◽  
Mark D. Wilkinson ◽  
...  
Keyword(s):  
Cell Walls ◽  
Endosperm Cell ◽  
Wheat Endosperm

Isolation and characterization of cell walls from the mesocarp of mature grape berries (Vitis vinifera)

2020 ◽  
Author(s):  
KJ Nunan ◽  
Ian Sims ◽  
A Bacic ◽  
SP Robinson ◽  
GB Fincher
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Vascular Tissue ◽  
Compositional Analysis ◽  
Water Soluble ◽  
Vitis Vinifera L ◽  
Nylon Mesh ◽  
Cytoplasmic Material ◽  
Isolation And Characterization ◽  
Tissue Homogenates

Cell walls have been isolated from the mesocarp of mature grape (Vitis vinifera L.) berries. Tissue homogenates were suspended in 80% (v/v) ethanol to minimise the loss of water-soluble wall components and wet-sieved on nylon mesh to remove cytoplasmic material. The cell wall fragments retained on the sieve were subsequently treated with buffered phenol at pH 7.0, to inactivate any wall-bound enzymes and to dislodge small amounts of cytoplasmic proteins that adhered to the walls. Finally, the wall preparation was washed with chloroform/methanol (1:1, v/v) to remove lipids and dried by solvent exchange. Scanning electron microscopy showed that the wall preparation was essentially free of vascular tissue and adventitious protein of cytoplasmic origin. Compositional analysis showed that the walls consisted of approximately 90% by weight of polysaccharide and less than 10% protein. The protein component of the walls was shown to be rich in arginine and hydroxyproline residues. Cellulose and polygalacturonans were the major constituents, and each accounted for 30-40% by weight of the polysaccharide component of the walls. Substantial varietal differences were observed in the relative abundance of these two polysaccharides. Xyloglucans constituted approximately 10% of the polysaccharide fraction and the remainder was made up of smaller amounts of mannans, heteroxylans, arabinans and galactans.

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