scholarly journals Changes in Germination Promotion and Inhibition in Seed Extracts of Subterranean Clover (Trifolium Subterraneum L.) Related to Dormancy and Germination

1971 ◽  
Vol 24 (4) ◽  
pp. 897 ◽  
Author(s):  
MG Walker
Keyword(s):  
Ethyl Acetate ◽  
Paper Chromatography ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Germination Inhibitors ◽  
Aqueous Fraction ◽  
Clover Seed ◽  
Seed Extracts

Seeds of two varieties of T. BUbterraneum were extracted after imbibing water for various periods. The extracts were partitioned into water and ethyl acetate, purified by paper chromatography, and germination.modifying activity measured by a subterranean clover seed bioassay. As imbibition proceeded, the balance of germination inhibitors and promo tors in the aqueous fraction remained fairly constant in the dormant variety, but changed "rapidly in the non-dormant variety. The activity in the ethyl acetate fractions of both varieties also changed rapidly, but for the dormant variety the final balance was inhibitory, and in the non-dormant variety promotory.

scholarly journals Harvesting subterranean clover seed – current practices, technology and issues

2021 ◽  
Vol 72 (3) ◽  
pp. 223
Author(s):  
Wesley M. Moss ◽  
Andrew L. Guzzomi ◽  
Kevin J. Foster ◽  
Megan H. Ryan ◽  
Phillip G. H. Nichols
Keyword(s):  
Soil Erosion ◽  
Case Studies ◽  
Seed Production ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Root Cause ◽  
Pasture Plant ◽  
Clover Seed ◽  
Production Industry ◽  
Pasture Legume

Subterranean clover (Trifolium subterraneum L.) is Australia’s most widely sown annual pasture legume. Its widespread use as a pasture plant requires a well-functioning seed production industry, and Australia is the only significant producer of subterranean clover seed globally. However, the sustainability of this industry is under threat due to its reliance on ageing harvest equipment and the resultant environmental impacts. In order to evaluate seed harvesting practices, technology, and issues, we report on case studies, workshops, and a survey of seed producers across southern Australia. The Horwood Bagshaw Clover Harvester, designed in the 1950s, remains the most popular subterranean clover seed harvester. We discuss its use and modifications, and document several contemporary issues facing the seed production industry. Issues are primarily soil erosion and degradation; the expensive, slow and labour-intensive harvest process; and poor reliability and maintainability of harvesters that are now at least 30 years old. We conclude the root cause of these issues is the suction harvest technology utilised by the Horwood Bagshaw Clover Harvester. Analysis of the current harvest system is provided to support the development of new approaches to harvest subterranean clover seeds.

Effect of perennial pasture species on surface soil moisture and early growth and survival of subterranean clover (Trifolium subterraneum L.) seedlings

1997 ◽  
Vol 48 (5) ◽  
pp. 683 ◽  
Author(s):  
B. S. Dear ◽  
P. S. Cocks
Keyword(s):  
Mineral Soil ◽  
Subterranean Clover ◽  
Late Summer ◽  
Soil Surface ◽  
Trifolium Subterraneum ◽  
Annual Grass ◽  
Growth And Survival ◽  
Eastern Australia ◽  
Standing Dead ◽  
Clover Seed

Subterranean clover seedling numbers and growth in swards containing 1 of 5 perennial pasture species [phalaris (Phalaris aquatica) cv. Sirolan, cocksfoot (Dactylis glomerata) cv. Currie, lucerne (Medicago sativa) cv. Aquarius, wallaby grass (Danthonia richardsonii) cv. Taranna, and lovegrass (Eragrostis curvula) cv. Consol] were compared with those in typical annual pastures and pure clover swards in the wheatbelt of eastern Australia. Presence of a perennial species or the volunteer annual grass (Eragrostis cilianensis) increased the rate of drying of the soil surface (0–5 cm) after late February and May rain, compared with subterranean clover swards. Perennials differed in the rate they dried the soil surface, with the more summer-active lucerne and consul lovegrass drying the profile more rapidly than phalaris. The amount of water in the surface 5 cm, 6 days after the rainfall event on 27–28 February, was strongly negatively correlated (r = –0·75, P < 0·01) with the amount of green perennial biomass, but not related to standing dead material or surface residues. Where perennials were present, a smaller proportion (2–4%) of the clover seed pool produced seedlings in response to late summer rain, compared with pure clover swards (18%). A higher proportion of the seed pool produced seedlings (19–36%) following rain in late autumn but there was no difference between species. The more summer-active perennials (cocksfoot, danthonia, and lucerne) markedly depressed the survival of emerged clover seedlings following both germinations. Of the seedlings that emerged in early March, the proportion remaining by 29 March was 57% in phalaris, 21% in lucerne, 13% in danthonia, and 1% in cocksfoot, compared with a 78% increase in seedlings in pure subterranean clover swards. By 15 May, all perennials had <2 clover seedlings/m2 surviving, compared with 37 in the annual pasture and 964 plants/m2 in pure subterranean clover. Following the May germination, the highest proportion of emerged seedlings surviving until 29 May was in the phalaris swards (40%) and least in the cocksfoot and danthonia swards (2–4%). Presence of a perennial or annual grass decreased (P < 0·05) relative water content of clover seedlings on 15 March from 74% in pure clover swards, to 48% in annual pasture, 34% in phalaris, and 29% in lucerne swards. Clover seedlings growing in pure subterranean swards on 15 March (17 days after germinating rain) were 4 times larger than those in lucerne and twice as large as those in either phalaris or annual pasture. Seed size did not differ between treatments, but available mineral soil nitrogen was significantly higher (P < 0·001) in pure subterranean clover swards (32 mg N/g) compared with perennials (3–13 mg N/g). Strategies such as heavy grazing in late summer to reduce green biomass of the perennials or sowing the perennials at lower densities may reduce the adverse effects that perennials have on subterranean clover seedlings in these drier environments.

Lucerne, phalaris, and wallaby grass in short-term pasture phases in two eastern Australian wheatbelt environments. 2. Effect of perennial density and species on subterranean clover populations and the relative success of 3 clover cultivars of different maturity

2007 ◽  
Vol 58 (2) ◽  
pp. 123 ◽  
Author(s):  
B. S. Dear ◽  
G. A. Sandral ◽  
J. M. Virgona ◽  
A. D. Swan ◽  
B. A. Orchard ◽  
...  
Keyword(s):  
Seed Yield ◽  
Seed Bank ◽  
Seedling Survival ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Climatic Conditions ◽  
Perennial Species ◽  
Growing Season Length ◽  
Herbage Yield ◽  
Clover Seed

The effect of the density of 3 perennial species, phalaris (Phalaris aquatica L.), wallaby grass (Austrodanthonia richardsonii Kunth), and lucerne (Medicago sativa L.), on seed set, regeneration, and the relative competitiveness of 3 cultivars of subterranean clover (Trifolium subterraneum L.) was examined in 2 environments in the south-eastern Australian wheatbelt. Seed yields of subterranean clover were inversely related to perennial density at both sites over the first 2 years, the relationship varying with perennial species. Phalaris depressed the seed yield of clover more than lucerne and wallaby grass in the second and third year at equivalent densities. Clover seed yield was positively related to clover herbage yield in late spring at both sites, and inversely related to perennial herbage yield. Clover seed yield displayed an increasing linear relationship with the proportion of light reaching the clover understorey in spring, which in turn was inversely related to perennial density and perennial herbage yield. Clover seedling regeneration in mixed swards in autumn was positively related to the size of the summer seed bank, but negatively related to perennial density. Clover seedling survival following a premature germination at Kamarah was inversely correlated to the density of phalaris and lucerne in the sward. The relative competitiveness of the 3 subterranean clover cultivars varied between sites, with climatic conditions (rainfall and growing-season length) having a greater effect on the relative cultivar performance than companion perennial species or density. The later maturing subterranean clover cv. Goulburn became the dominant cultivar at the wetter site, constituting 72% of the seed bank, but declined to only 3–8% of the seed bank at the drier site. The proportion of the early flowering cultivar Dalkeith in the seed bank increased over time at the drier site and was highest (53%) in plots with the highest perennial density. We concluded that although perennial pasture species will depress clover seed yield and subsequent regeneration, these effects could be minimised by reducing perennial densities and exploiting variations in competitiveness between perennial species as identified in this study. Sowing earlier maturing subterranean clover cultivars would only be an advantage in increasing clover content in low-rainfall environments. The findings suggest that clover seed reserves and regeneration could also be increased by using grazing management to reduce the level of shading of clover by perennials, a factor associated with reduced clover seed yield.

Effect of tillage practices on the fate of hard seeds of subterranean clover in a ley farming system

1985 ◽  
Vol 25 (3) ◽  
pp. 568 ◽  
Author(s):  
GB Taylor
Keyword(s):  
Subterranean Clover ◽  
Soil Surface ◽  
Trifolium Subterraneum ◽  
Farming System ◽  
Conventional Tillage ◽  
No Tillage ◽  
Minimum Tillage ◽  
Tillage Practices ◽  
Tillage Treatment ◽  
Clover Seed

In a rotation of 1 year pasture/l year crop, a subterranean clover (Trifolium subterraneum cv. Daliak) pasture was either left untilled or subjected to minimum or conventional tillage. One set of tillage treatments was imposed in each ofthree crop years while another set of treatments was imposed in only the first crop year. Regenerating clover plants were prevented from setting seed. In the first crop, 44% of the clover seeds were buried below 2 cm of soil by minimum tillage; this proportion was 65% in the conventional tillage treatment. In the first pasture regeneration year, seedling densities were highest in the no-tillage treatment. Conversely, there were more residual seeds in the tilled treatments and, in the second and third pasture regeneration years, this led to higher seedling densities than in the no-tillage treatment. The effects of tillage were more marked in the conventional than in the minimum-tillage treatment. Clover establishment was improved by repeat tillage operations which returned some of the buried seeds closer to the soil surface. Although more seedlings overall were obtained from the no-tillage treatment, the disadvantage of fewer seedlings in the tilled treatments was offset by the spread of seedling establishment over a number of pasture years. This spread, which would be more marked with harder-seeded cultivars, could be desirable in environments in which clover seed production is unreliable.

Boron deficiency in pasture based on subterranean clover (Trifolium subterraneum L.) is linked to symbiotic malfunction

2015 ◽  
Vol 66 (11) ◽  
pp. 1197 ◽  
Author(s):  
Leo J. Hamilton ◽  
Kevin F. M. Reed ◽  
Elainne M. A. Leach ◽  
John Brockwell
Keyword(s):  
N2 Fixation ◽  
Rhizobium Leguminosarum ◽  
Root Nodule ◽  
Effective Strain ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Boron Deficiency ◽  
Initial Application ◽  
Clover Root ◽  
Clover Seed

Field and glasshouse experiments confirmed the occurrence of boron (B) deficiency in subterranean clover (Trifolium subterraneum L.) pasture in eastern Victoria. Diminished productivity was linked to the small-seededness of clover and the poor effectiveness of clover root-nodule bacteria (rhizobia, Rhizobium leguminosarum bv. trifolii). Productivity, especially of clover and clover seed, increased following applications of up to 6 kg B ha–1 (P < 0.001). The response was delayed, occurring several years after the initial application of B, unless the land was resown with fresh clover seed inoculated with an effective strain of rhizobia. B deficiency in the nodulated legume induced conditions within the plant and or its rhizobia that led to impaired nitrogen (N2) fixation. Glasshouse research indicated that populations of soil-borne rhizobia taken from B-deficient soils were poorly effective in N2 fixation and that rhizobia from soils growing subterranean clover cv. Leura were significantly less effective (P < 0.05) than rhizobia from a soil growing cv. Mt Barker. Additionally, subterranean clover seed generated in B-deficient soils was at least one-third smaller than the seed of commercial seed but responded to inoculation with effective rhizobia. This indicated that any symbiotic malfunction of clover from B-deficient soils was not due to an inability to respond to nitrogen per se. On the other hand, cv. Leura from B-deficient soils fixed significantly less N2 than commercial cv. Leura when each was inoculated with rhizobia from B-deficient soils.

Studies on nodulation responses to pelleting of subterranean clover seed.

1961 ◽  
Vol 12 (4) ◽  
pp. 578 ◽  
Author(s):  
JA Thompson
Keyword(s):  
Seed Coat ◽  
Field Experiments ◽  
Chemical Constituents ◽  
Chemical Properties ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Coating Materials ◽  
Physical Separation ◽  
Clover Seed

In a series of field experiments on seven soils of pH 6.0 or higher, nodulation of subterranean clover (Trifolium subterraneum L. var. Tallarook) was improved by pelleting the seed with various glues and coating materials, prior to inoculation with dry peat inoculum. On these soils the responses were not related to the chemical properties of the pellet materials. The beneficial effect was apparently the result of physical separation of the seed coat and inoculum. It is postulated that pelleting of seed protects the inoculum from an antibiotic, whlch has been found in subterranean clover seed coats, and which is active against Rhizobium in culture. On a soil of pH 5.1 a nodulation response to two types of pelleting materials was apparently related to the chemical constituents of the coating materials. In this soil there was less evidence of the importance of physical separation of the inoculum from the seed coat. It seems likely that soils may differ considerably in their ability to inactivate the antibiotic. Nodulation was not improved by in situ fumigation, prior to sowing, of two soils of pH 6.0 and 6.6, which indicated that antagonism by soil microorganisms was not the cause of poor nodulation in these soils. Significant nodulation responses to pelleting were obtained in sowings in these fumigated soils.

Distribution and abundance of annual legume seeds in the wheatbelt of Western Australia

1995 ◽  
Vol 35 (2) ◽  
pp. 189 ◽  
Author(s):  
JA Fortune ◽  
PS Cocks ◽  
CK Macfarlane ◽  
FP Smith
Keyword(s):  
Western Australia ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Farming Systems ◽  
Annual Rainfall ◽  
Soil Parameters ◽  
Widespread Species ◽  
Legume Seeds ◽  
Clover Seed

The size and composition of pasture legume seedbanks were estimated from 2 surveys on a 460-km west-east transect of the wheatbelt of Western Australia. Survey 1 (in spring) sampled naturalised legumes, and survey 2 (in summer) measured the amount and botanical composition of legume seed from selected sites. Seedbanks were examined in greater detail on 2 farms in the higher rainfall part of the wheatbelt. Survey 2 revealed that mean seedbank size of the poorest 40% of sites (those with 5200 kg seed/ha) was 61 kg/ha, and that 72% of seeds were naturalised clovers. In contrast, the best 60% of sites (those with >200 kg seed/ha) averaged 533 kg seed/ha, of which only 35% was naturalised clover seed, the remainder in both surveys being mainly subterranean clover (Trifolium subterraneum). Mean seed bank size (kg/ha) varied from 359 (survey 2) to 587 (survey 1) and, in both surveys, was poorly correlated with long-term mean annual rainfall and a number of soil parameters. On the 2 farms, seedbank size ranged from 300 to 345 kg/ha (in spring) and from 650 to 740 kg/ha (in summer). Trifolium glomeratum (cluster clover) and subterranean clover were the most widespread species in both surveys. They were present at 35 and 30 of the 57 survey sites, respectively, and at both farms. Most of the subterranean clover collected was cv. Geraldton (22 of 30 sites), the next most frequent cultivar was Dwalganup (6 sites). The currently recommended cultivar, Dalkeith, was found at only 5 sites. Several other legumes including T. tomentosum (16 sites), T. suffocatum (8 sites), Medicago truncatula (7 sites), T. hirtum (4 sites), and M. minima (4 sites) were common, while M. littoralis, M. polymorpha, T. dubium, T. cernuum, T. cherleri, and T. carnpestre were found at single sites. With few exceptions, these are naturalised species and were characterised by flowering times about 20 days later than sown legume cultivars, and seed sizes < 1 mg. The value of these widespread annual legumes to agricultural productivity and sustainability needs to be quantified and their adaptation to wheatbelt farming systems assessed.

Factors affecting the productivity of irrigated annual pastures. 2. Defoliation by dairy cows

1986 ◽  
Vol 26 (3) ◽  
pp. 305 ◽  
Author(s):  
CR Stockdale
Keyword(s):  
Grazing Intensity ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Total Production ◽  
Pasture Production ◽  
Factors Affecting ◽  
Grazing Intensities ◽  
Seed Reserves ◽  
Clover Seed

The influence of grazing intensity on the productivity of an irrigated annual pasture was studied for 3 years in northern Victoria. Lax-, medium- and hard-grazing intensities were described by post-grazing pasture heights of 7.2, 5.2 and 3.0 cm, respectively. Also, one instance of variable grazing frequency occurred, in winter of year 1. Hard-grazed plots produced 1 3 and 17% less herbage in years 1 and 2, respectively, than did lax- and medium-grazed plots, which produced similar amounts of herbage. When the interval between grazings was extended, the variation in productivity was reversed; lax grazing resulted in 9% less total production than heavier grazing. In years 1 and 2, there was little effect of grazing treatment on botanical composition until spring, at which time there was a marked reduction in the amount of subterranean clover (Trifolium subterraneum) in the hard-grazed plots, with a concomitant increase in grass content. There were no significant effects of grazing intensity on the amounts of weeds in either year. However, in year 3, weeds were important contributors to pasture production early in the season. This, together with reduced clover seed reserves and increased incidence of disease in subterranean clover with hard grazing, suggests that the long-term regenerating ability of an annual pasture may be impaired if severely grazed at regular intervals.

Bean leafroll virus is widespread in subterranean clover (Trifolium subterraneum L.) seed crops and can be persistently transmitted by bluegreen aphid (Acyrthosiphon kondoi Shinji)

2012 ◽  
Vol 63 (9) ◽  
pp. 902 ◽  
Author(s):  
D. M. Peck ◽  
N. Habili ◽  
R. M. Nair ◽  
J. W. Randles ◽  
C. T. de Koning ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Seed Production ◽  
Alfalfa Mosaic Virus ◽  
South Australia ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Bean Leafroll Virus ◽  
Leafroll Virus ◽  
Clover Seed

In the mid 2000s subterranean clover (Trifolium subterraneum) seed producers in South Australia reported symptoms of a red-leaf disease in fields with reduced seed yields. The red-leaf symptoms resembled those caused by several clover-infecting viruses. A set of molecular diagnostic tools were developed for the following viruses which are known to infect subterranean clover: Alfalfa mosaic virus; Bean leafroll virus (BLRV); Beet western yellows virus; Bean yellow mosaic virus; Cucumber mosaic virus; Pea seed-borne mosaic virus; Soybean dwarf virus and Subterranean clover stunt virus. Surveys of subterranean clover seed production fields in 2008 in the south-east of South Australia and western Victoria identified Bean leafroll virus, Alfalfa mosaic virus and Cucumber mosaic virus as present, with BLRV the most widespread. Surveys of pasture seed production fields and pasture evaluation trials in 2009 confirmed that BLRV was widespread. This result will allow seed producers to determine whether control measures directed against BLRV will overcome their seed losses. Bluegreen aphid (Acyrthosiphon kondoi) was implicated as a potential vector of BLRV because it was observed to be colonising lucerne plants adjacent to subterranean clover seed production paddocks with BLRV, and in a glasshouse trial it transmitted BLRV from an infected lucerne plant to subterranean clover in a persistent manner.

Influence of cultivar and season on the response of Trifolium subterraneum to simazine

1992 ◽  
Vol 32 (8) ◽  
pp. 1095 ◽  
Author(s):  
BS Dear ◽  
DJ Conlan ◽  
MF Richards ◽  
NE Coombes
Keyword(s):  
Seed Set ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Yield Response ◽  
Yield Losses ◽  
Lolium Rigidum ◽  
Herbage Yield ◽  
Annual Grasses ◽  
Clover Seed ◽  
Seed Yields

The tolerance of 6 cultivars of Trifolium subterraneum (subterranean clover) to simazine applied at 0.63 and 1.25 kg a.i./ha was determined under weedfree conditions in the field by measuring herbage and seed yields. Large herbage yield losses occurred as a result of the simazine in spring in the 2 years of the experiments. In 1989, spring herbage yield losses of the cultivars at the 0.63 and 1.25 kg/ha simazine rates averaged 56 and 82%, respectively. In 1990 the spring herbage yield losses were 27 and 51%. Significant variation in tolerance was observed between cultivars in both years, with Trikkala being the most tolerant and Karridale the most susceptible cultivar. Rate of herbicide had the greatest effect on herbage yield, with cultivar having a smaller effect. In 1989, with relatively favourable spring conditions, clover seed yields were depressed by simazine, but in 1990 when drier conditions prevailed during flowering and seed set, seed yields were unaffected or slightly increased by simazine in all cultivars except the early-flowering cultivar Dalkeith. Seed yields of simazine-treated swards were 196-1480 kg/ha in 1989 and 359-686 kg/ha in 1990. The seed yield response in 1990 suggests that herbicides which retard growth in winter help to conserve soil water and, therefore, may benefit seed filling later in the season. The presence of Lolium rigidum at spraying did not reduce the effect of the herbicide on clover herbage yield and had no effect on seed set. Although simazine may reduce herbage yields and, in some cases, seed yields, its use may be justified for the control of annual grasses when other factors such as disease control, pasture quality, and level of weed competition are considered.

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