scholarly journals Regulation of Recombination at the His-3 Locus in Neurospora Crassa

1970 ◽  
Vol 23 (5) ◽  
pp. 1229 ◽  
Author(s):  
Teresa Angel ◽  
Barbara Austin ◽  
DG Catcheside
Keyword(s):  
Linkage Group ◽  
Neurospora Crassa ◽  
Dominant Gene ◽  
Group V

The frequency of prototrophic recombination between pairs of his-3 alleles is increased in the absence of the dominant gene reo-w+, which is probably the same as reo-4+. The locus of reo-w is in linkage group V. The degree of increase is determined by genes at a reoognition locus (oog) situated about 1� 3 units distally to the hi8-3 locus. In the presence of oog+, derived from Y8743 which has Lindegren wild stocks as ancestors, the increase is about 30-fold.

Recombinational landscape across a 650-kb contig on the right arm of linkage group V in Neurospora crassa

1999 ◽  
Vol 36 (5) ◽  
pp. 270-274 ◽  
Author(s):  
Frederick J. Bowring ◽  
David E. A. Catcheside
Keyword(s):  
Linkage Group ◽  
Neurospora Crassa ◽  
Group V ◽  
The Right

scholarly journals Structural gene for ornithine decarboxylase in Neurospora crassa

1985 ◽  
Vol 5 (6) ◽  
pp. 1301-1306
Author(s):  
P Eversole ◽  
J J DiGangi ◽  
T Menees ◽  
R H Davis
Keyword(s):  
Linkage Group ◽  
Neurospora Crassa ◽  
Ornithine Decarboxylase ◽  
Structural Gene ◽  
Minimal Medium ◽  
Temperature Sensitive ◽  
Wild Type ◽  
Wild Type Enzyme ◽  
Group V

To define the structural gene for ornithine decarboxylase (ODC) in Neurospora crassa, we sought mutants with kinetically altered enzyme. Four mutants, PE4, PE7, PE69, and PE85, were isolated. They were able to grow slowly at 25 degrees C on minimal medium but required putrescine or spermidine supplementation for growth at 35 degrees C. The mutants did not complement with one another or with ODC-less spe-1 mutants isolated in earlier studies. In all of the mutants isolated to date, the mutations map at the spe-1 locus on linkage group V. Strains carrying mutations PE4, PE7, and PE85 displayed a small amount of residual ODC activity in extracts. None of them had a temperature-sensitive enzyme. The enzyme of the PE85 mutant had a 25-fold higher Km for ornithine (5mM) than did the enzyme of wild-type or the PE4 mutant (ca. 0.2 mM). The enzyme of this mutant was more stable to heat than was the wild-type enzyme. These characteristics were normal in the mutant carrying allele PE4. The mutant carrying PE85 was able to grow well at 25 degrees C and weakly at 35 degrees C with ornithine supplementation. This mutant and three ODC-less mutants isolated previously displayed a polypeptide corresponding to ODC in Western immunoblots with antibody raised to purified wild-type ODC. We conclude that spe-1 is the structural gene for the ODC.

scholarly journals LINKAGE RELATIONS OF NEW MORPHOLOGICAL MUTANTS IN LINKAGE GROUP V OF NEUROSPORA CRASSA

Genetics ◽  
1967 ◽  
Vol 57 (3) ◽  
pp. 605-612 ◽  
Author(s):  
M P Morgan ◽  
L Garnjobst ◽  
E L Tatum
Keyword(s):  
Linkage Group ◽  
Neurospora Crassa ◽  
Group V ◽  
Morphological Mutants

Position of Linkage Group V Markers in Chromosome 2 of Neurospora crassa

1969 ◽  
Vol 60 (3) ◽  
pp. 120-125 ◽  
Author(s):  
EDWARD G. BARRY ◽  
DAVID D. PERKINS
Keyword(s):  
Linkage Group ◽  
Neurospora Crassa ◽  
Chromosome 2 ◽  
Group V

scholarly journals Cytoplasmic leucyl-tRNA synthetase of Neurospora crassa is not specified by the leu-5 locus.

Genetics ◽  
1988 ◽  
Vol 119 (4) ◽  
pp. 805-814
Author(s):  
R Benarous ◽  
C M Chow ◽  
U L RajBhandary
Keyword(s):  
Linkage Group ◽  
Neurospora Crassa ◽  
Genomic Dna ◽  
Trna Synthetase ◽  
Cdna Clones ◽  
Far Right ◽  
Group V ◽  
Total N ◽  
Reading Frame ◽  
Atp Binding Site

Abstract We generated a lambda gt11 Neurospora crassa cDNA library and screened the library for the cytoplasmic leucyl-tRNA synthetase (cyto LeuRS) clones using cyto LeuRS specific antibody. Two clones, lambda NCLRSC1 and lambda NCLRSC2, were obtained which have inserts of approximately 2 kbp and approximately 1.3 kbp, and which overlap by about 0.6 kbp. The following lines of evidence indicate that lambda NCLRSC1 and lambda NCLRSC2 encode parts of cyto LeuRS. (1) Antibodies affinity purified using either of the fusion proteins encoded by lambda NCLRSC1 or lambda NCLRSC2 inhibit cyto LeuRS activity. Thus, the fusion protein and cyto LeuRS share immunological determinants. (2) The same antibodies also react with an approximately 115-kDa protein, which comigrates with purified cyto LeuRS, in immunoblots of total N. crassa proteins. We used the cDNA clones to probe a N. crassa genomic DNA library and isolated two genomic DNA clones. Partial sequence analysis of cDNA and genomic DNA clones shows a methionine initiated open reading frame, which includes a stretch of amino acid residues that are highly conserved and that are at the ATP binding site in aminoacyl-tRNA synthetases. Using the cloned DNA as probe, we show that the cyto LeuRS mRNA is approximately 3900 nucleotides long. Finally, we have used restriction fragment length polymorphism mapping to show that the cyto LeuRS gene resides on the far right of linkage group II and not on linkage group V where the leu-5 mutation, which was previously reported to specify cyto LeuRS, is located.

scholarly journals Structural gene for ornithine decarboxylase in Neurospora crassa.

1985 ◽  
Vol 5 (6) ◽  
pp. 1301-1306 ◽  
Author(s):  
P Eversole ◽  
J J DiGangi ◽  
T Menees ◽  
R H Davis
Keyword(s):  
Linkage Group ◽  
Neurospora Crassa ◽  
Ornithine Decarboxylase ◽  
Structural Gene ◽  
Minimal Medium ◽  
Temperature Sensitive ◽  
Wild Type ◽  
Wild Type Enzyme ◽  
Group V

To define the structural gene for ornithine decarboxylase (ODC) in Neurospora crassa, we sought mutants with kinetically altered enzyme. Four mutants, PE4, PE7, PE69, and PE85, were isolated. They were able to grow slowly at 25 degrees C on minimal medium but required putrescine or spermidine supplementation for growth at 35 degrees C. The mutants did not complement with one another or with ODC-less spe-1 mutants isolated in earlier studies. In all of the mutants isolated to date, the mutations map at the spe-1 locus on linkage group V. Strains carrying mutations PE4, PE7, and PE85 displayed a small amount of residual ODC activity in extracts. None of them had a temperature-sensitive enzyme. The enzyme of the PE85 mutant had a 25-fold higher Km for ornithine (5mM) than did the enzyme of wild-type or the PE4 mutant (ca. 0.2 mM). The enzyme of this mutant was more stable to heat than was the wild-type enzyme. These characteristics were normal in the mutant carrying allele PE4. The mutant carrying PE85 was able to grow well at 25 degrees C and weakly at 35 degrees C with ornithine supplementation. This mutant and three ODC-less mutants isolated previously displayed a polypeptide corresponding to ODC in Western immunoblots with antibody raised to purified wild-type ODC. We conclude that spe-1 is the structural gene for the ODC.

Genetical Studies on the Skeleton of the Mouse1

Development ◽  
1958 ◽  
Vol 6 (1) ◽  
pp. 124-148
Author(s):  
Hans Grüneberg
Keyword(s):  
Linkage Group ◽  
Genetic Background ◽  
Dominant Gene ◽  
Odontoid Process ◽  
Axial Skeleton ◽  
Cervical Region ◽  
Group V ◽  
Short Tail ◽  
Vertebral Bodies ◽  
General Reduction

The semi-dominant gene for Danforth's short-tail in the mouse (symbol Sd; linkage group V) was first described by Dunn, Gluecksohn-Schoenheimer, & Bryson (1940). The most conspicuous abnormality of Sd/+ heterozygotes is a shortening of the tail the extent of which varies with the genetic background (Dunn, 1942; Fisher & Holt, 1944; Dunn & Gluecksohn-Schoenheimer, 1945). Reduction or absence of kidneys is common on some genetic backgrounds, but rare or absent on others (Gluecksohn-Schoenheimer, 1943). Reduction or absence of the dens epistrophei (odontoid process of the axis) with formation of an anomalous articulation between atlas and epistropheus (axis) was later described by Theiler (1951 a, b; 1952; 1954) and by Grüneberg (1953). The reduction of the dens epistrophei is part and parcel of a general reduction of the vertebral bodies which is most marked in the cervical region, but which can be traced throughout the whole length of the axial skeleton.

scholarly journals REGULATION OF PHOSPHATE METABOLISM IN NEUROSPORA CRASSA: ISOLATION OF MUTANTS DEFICIENT IN THE REPRESSIBLE ALKALINE PHOSPHATASE

Genetics ◽  
1974 ◽  
Vol 78 (2) ◽  
pp. 645-659
Author(s):  
Mary K Gleason ◽  
Robert L Metzenberg
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Linkage Group ◽  
Neurospora Crassa ◽  
Heat Stability ◽  
Phosphate Metabolism ◽  
Wild Type ◽  
Wild Type Enzyme ◽  
Group V ◽  
Suppressor Mutations

ABSTRACT Mutants of Neurospora crassa have been isolated that lack the repressible alkaline phosphatase, but, unlike nuc-1 and nuc-2 mutants, are able to make the repressible acid phosphatase and the repressible phosphate permease under conditions of derepression (phosphate deprivation). The new mutants, called pho-2, map in Linkage Group V, and are unlinked to the putative control mutants, nuc-1, nuc-2-pconc, and pregc. Three of the pho-2 mutants do not make detectable amounts of repressible alkaline phosphatase, but the fourth makes about 1% of the level found in wild type. The small amount of alkaline phosphatase made by this strain appears to be qualitatively similar or identical to the wild-type enzyme, as judged by electrophoretic mobility, heat stability, and titration with specific antibody to the wild-type enzyme. Several revertants of this strain have been examined in the same way, and the alkaline phosphatase of these strains also appears to be qualitatively normal. Reversion events can occur at, or near, the pho-2 locus, but also occur in at least two unlinked sites (suppressor mutations). One suppressor maps very close to nuc-1.

scholarly journals REGULATION OF PHOSPHATE METABOLISM IN NEUROSPORA CRASSA: IDENTIFICATION OF THE STRUCTURAL GENE FOR REPRESSIBLE ALKALINE PHOSPHATASE

Genetics ◽  
1976 ◽  
Vol 84 (2) ◽  
pp. 175-182
Author(s):  
John F Lehman ◽  
Robert L Metzenberg
Keyword(s):  
Alkaline Phosphatase ◽  
Linkage Group ◽  
Neurospora Crassa ◽  
Structural Gene ◽  
Type A ◽  
Phosphate Metabolism ◽  
Wild Type ◽  
Wild Type Enzyme ◽  
Group V ◽  
Low Levels

ABSTRACT Five additional mutants of Neurospora crassa have been isolated that lack the repressible alkaline phosphatase. The mutations in these strains map at a previously assigned locus on Linkage Group V designated pho-2 (Gleason and Metzenberg 1974). The five new mutants, as well as three previously isolated by Gleason and Metzenberg (1974), were examined for the presence of cross-reacting material to antibody prepared against purified wild-type enzyme. Two of the mutants produced high levels of cross-reacting material, thus providing evidence that the pho-2 locus includes the structural gene for the repressible alkaline phosphatase. Two revertants were obtained from one of the mutants that contained cross-reacting material. Neither revertant produced an enzyme that could be distinguished physicochemically from that of wild type. A method for measuring very low levels of repressible alkaline phosphatase in crude extracts is also described.

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