scholarly journals The Effect of Cycloheximide on Cell Division in Partially Synchronized Plant Cells

1970 ◽  
Vol 23 (3) ◽  
pp. 573 ◽  
Author(s):  
RJ Rose
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Broad Bean ◽  
Plant Cells ◽  
Jerusalem Artichoke ◽  
Root Tips ◽  
Tuber Tissue ◽  
Jerusalem Artichoke Tuber ◽  
Bean Root ◽  
Mitotic Frequency

Cycloheximide blocked cell division at interphase, prophase, prometaphase, metaphase, and telophase in Jerusalem artichoke tuber tissue. Blocking at anaphase did not occur or was very infrequent. In any cell, the location of the block depended upon the stage of the cell cycle reached at the time of cycloheximide addition. In general, cycloheximide caused similar blocks in interphase and mitosis in cells of synchronised broad bean root tips. A reduction of mitotic frequency occurred after about 8 hr in cycloheximide, indicating a reversion of chromosome coiling.

scholarly journals Cell Cycle-Specific Disruption of the Preprophase Band of Microtubules in Fern Protonemata: Effects of Displacement of the Endoplasm by Centrifugation

1992 ◽  
Vol 101 (1) ◽  
pp. 93-98 ◽  
Author(s):  
TAKASHI MURATA ◽  
MASAMITSU WADA
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Plant Cells ◽  
Preprophase Band ◽  
Higher Plant ◽  
The Future ◽  
Prophase Nucleus ◽  
Original Region ◽  
Fern Protonemata

The preprophase band (PPB) of microtubules (MTs), which appears at the future site of cytokinesis prior to cell division in higher plant cells, disappears by metaphase. Recent studies have shown that displacement of the endoplasm from the PPB region by centrifugation delays the disappearance of the PPB. To study the role of the endoplasm in the cell cycle-specific disruption of the PPB, the filamentous protonemal cells of the fern Adiantum capilius-veneris L. were centrifuged twice so that the first centrifugation displaced the endoplasm from the site of the PPB and the second returned it to its original location. The endoplasm, including the nucleus of various stages of mitosis, could be returned by the second centrifugation to the original region of the PPB, which persists during mitosis in the centrifuged cells. When endoplasm with a prophase nucleus was returned to its original location, the PPB was not disrupted. When endoplasm with a prometa-phase telophase nucleus was similarly returned, the PPB was disrupted within 10 min of termination of centrifugation. In protonemal cells of Adiantum, a second PPB is often formed near the displaced nucleus after the first centrifugation. In cells in which the endoplasm was considered to have been returned to its original location at the prophase/prometaphase transition, the second PPB did not disappear even though the initial PPB was disrupted by the endoplasm. These results suggest that cell cycle-specific disruption of the PPB is regulated by some factor(s) in the endoplasm, which appears at prometaphase, i.e. the stage at which the PPB is disrupted in non-centrifuged cells.

Effects of 2,4- and 3,5-dichlorophenoxyacetic acids on Jerusalem artichoke tuber tissue disks

1969 ◽  
Vol 8 (10) ◽  
pp. 1859-1866 ◽  
Author(s):  
P.P. Rutherford ◽  
E.W. Weston ◽  
A.E. Flood
Keyword(s):  
Jerusalem Artichoke ◽  
Tuber Tissue ◽  
Jerusalem Artichoke Tuber
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