scholarly journals Fungal Cellulases XXI. Induction of the Cellulase of Staghybotrys Atra

1967 ◽  
Vol 20 (1) ◽  
pp. 221 ◽  
Author(s):  
MA Jermyn
Keyword(s):  
Protein Synthesis ◽  
Hydroxyethyl Cellulose ◽  
Natural Growth ◽  
Chain Polymer ◽  
Added Sugars ◽  
Ethyl Hydroxyethyl Cellulose ◽  
Lag Period ◽  
Stachybotrys Atra ◽  
Kinetics Of ◽  
Long Chain

The use of Modocoll M (ethyl hydroxyethyl cellulose) as a soluble inducing agent for the cellulase of Stachybotrys atra is described. Some characteristics both of the kinetics of the induction and its inhibition have been determined. There is a lag period of 2�5 hr in the induction at 28�C for peptone-grown mycelium, and added sugars appear to be competitive with the inducer. These two characteristics have previously been noted as peculiarities in the induction of aryl ,a-glucosidase. The induction is also dependent on the continued presence of long-chain polymer. Inhibition experiments suggest that the normal processes of induced protein synthesis are operating in all other particulars, although the details of this inhibition are different from those observed in cellobiase induction. The way in which cellobiase and cellulase induction may be integrated during natural growth on cellulose is discussed.

scholarly journals The cellular origin of glyoxysomal proteins in germinating castor-bean endosperm

1976 ◽  
Vol 154 (2) ◽  
pp. 501-506 ◽  
Author(s):  
L Bowden ◽  
J. M Lord
Keyword(s):  
Endoplasmic Reticulum ◽  
Protein Synthesis ◽  
Castor Bean ◽  
Developmental Stages ◽  
Endoplasmic Reticulum Membrane ◽  
Endosperm Tissue ◽  
Cellular Origin ◽  
Lag Period ◽  
Kinetics Of

The capacity of castor-bean endosperm tissue to incorporate [35S]methionine into proteins of the total particulate fraction increased during the first 3 days of germination and subsequently declined. At the onset of germination 66% of the incorporated 35S was found in the separated endoplasmic-reticulum fraction, with the remainder in mitochondria, whereas at later developmental stages an increasing proportion of 35S was recovered in glyoxysomes. The kinetics of [35S]methionine incorporation into the major organelle fractions of 3-day-old endosperm tissue showed that the endoplasmic reticulum was immediately labelled, whereas a lag period preceded the labelling of mitochondria and glyoxysomes. When kinetic experiments were interrupted by the addition of an excess of unlabelled methionine, incorporation of [35S]methionine into the endoplasmic reticulum rapidly ceased, but incorporation into mitochondia and glyoxysomes continued for a further 1h. Examination of isolated organelle membranes during this period showed that the addition of unlabelled methionine resulted in a stimulated incorporation of [35S]no methionine into the endoplasmic-reticulum membrane for 30 min, after which time the 35S content of this fraction declined, whereas that of the glyoxysomal membranes continued to increase slowly. The 35S-labelling kinetics of organelles and fractions derived therefrom are discussed in relation to the role of the endoplasmic reticulum in protein synthesis during glyoxysome biogenesis.

scholarly journals Fungal Cellulases XX. Some Observations on the Induction and Inhibition of the Cellobiase of Stachybotrys Atra

1967 ◽  
Vol 20 (1) ◽  
pp. 193 ◽  
Author(s):  
MA Jermyn
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Information Transfer ◽  
Active Phase ◽  
Sugar Residue ◽  
Classical Pattern ◽  
Amino Acid Analogues ◽  
Lag Period ◽  
A Chain ◽  
Stachybotrys Atra

The induction of cellobiase in washed suspensions of Stachybotrys atra mycelium harvested during the active phase of growth follows the classical pattern for induced protein synthesis, except that it does not appear to be dependent on continued growth of the organism. At 28�C under aerobic conditions there is no detectable lag period. Inducers are ,B.glucosides of polyhydroxylic aglycones, which may be another sugar residue or a chain of such residues. Such inducers are metabolizable by the mould; after their breakdown the enzyme rapidly disappears from the mycelium. Interference with RNA metabolism and information transfer depresses the induced synthesis of the enzyme; two amino acid analogues, ,B-chloroalanine and p-fiuorophenylalanine, also inhibit this induction. Inhibition is also observed when respirable substances are present, and the inhibitor appears to arise from the metabolism of pyruvate.

Kinetics of Protein Synthesis in Enucleate Frog Oocytes

Science ◽  
1968 ◽  
Vol 160 (3832) ◽  
pp. 1115-1117 ◽  
Author(s):  
R. E. Ecker ◽  
L. D. Smith ◽  
S. Subtelny
Keyword(s):  
Protein Synthesis ◽  
Kinetics Of ◽  
Kinetics Of Protein Synthesis

scholarly journals Identification in skeletal muscle of a distinct extracellular pool of amino acids, and its role in protein synthesis

1971 ◽  
Vol 121 (5) ◽  
pp. 817-827 ◽  
Author(s):  
R. C. Hider ◽  
E. B. Fern ◽  
D. R. London
Keyword(s):  
Skeletal Muscle ◽  
Amino Acids ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Incubation Medium ◽  
Extensor Digitorum Longus ◽  
Extensor Digitorum ◽  
Longus Muscle ◽  
Kinetics Of

1. The kinetics of radioactive labelling of extra- and intra-cellular amino acid pools and protein of the extensor digitorum longus muscle were studied after incubations with radioactive amino acids in vitro. 2. The results indicated that an extracellular pool could be defined, the contents of which were different from those of the incubation medium. 3. It was concluded that amino acids from the extracellular pool, as defined in this study, were incorporated directly into protein.

scholarly journals Decomposition of time-dependent fluorescence signals reveals codon-specific kinetics of protein synthesis

2018 ◽  
Vol 46 (22) ◽  
pp. e130-e130 ◽  
Author(s):  
Nadin Haase ◽  
Wolf Holtkamp ◽  
Reinhard Lipowsky ◽  
Marina Rodnina ◽  
Sophia Rudorf
Keyword(s):  
Protein Synthesis ◽  
Time Dependent ◽  
Kinetics Of ◽  
Kinetics Of Protein Synthesis
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