scholarly journals The Determination of the Salt Relations of the Cytoplasmic Phase in Cells of Beetroot Tissue

1963 ◽  
Vol 16 (3) ◽  
pp. 647 ◽  
Author(s):  
MG Pitman
Keyword(s):  
Free Space ◽  
Cell Type ◽  
In Cells

An analysis of the rate at which isotope diffuses out of disks of beetroot tissue shows that there are at least two components of the non-free space. As tlwse components are not due to differences in cell type within the tissue, it is suggested they are due to a cytoplasmic phase in the parenchymatou's cells, and to the vacuoles.

Utilization of Labelled ‘Myleran’ for the Determination of the Size of DNA Molecules in Cells

Nature ◽  
1964 ◽  
Vol 203 (4947) ◽  
pp. 865-867 ◽  
Author(s):  
W. G. VERLY ◽  
A. PETITPAS-DEWANDRE
Keyword(s):  
Dna Molecules ◽  
In Cells

Determination of topological structure of ARL6ip1 in cells: Identification of the essential binding region of ARL6ip1 for conophylline

FEBS Letters ◽  
2013 ◽  
Vol 587 (22) ◽  
pp. 3656-3660 ◽  
Author(s):  
Masahiro Kuroda ◽  
Shintaro Funasaki ◽  
Tsuyoshi Saitoh ◽  
Yukiko Sasazawa ◽  
Shigeru Nishiyama ◽  
...  
Keyword(s):  
Topological Structure ◽  
Binding Region ◽  
In Cells

Non-contact-based determination of membrane permeability to water and dimethyl sulfoxide of cells virtually trapped in a self-induced micro-vortex

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Hsiu-Yang Tseng ◽  
Chiu-Jen Chen ◽  
Zong-Lin Wu ◽  
Yong-Ming Ye ◽  
Guo-Zhen Huang
Keyword(s):  
Cell Membrane ◽  
Dimethyl Sulfoxide ◽  
Membrane Permeability ◽  
Cell Membrane Permeability ◽  
Cell Type ◽  
Cryoprotective Agents ◽  
A Cell

Cell-membrane permeability to water (Lp) and cryoprotective agents (Ps) of a cell type is a crucial cellular information for achieving optimal cryopreservation in the biobanking industry. In this work, a...

scholarly journals The current distribution in an electrochemical cell. Part VII. Concluding remarks

2002 ◽  
Vol 67 (4) ◽  
pp. 273-278 ◽  
Author(s):  
Konstantin Popov ◽  
S.M. Pesic ◽  
Predrag Zivkovic
Keyword(s):  
Process Parameters ◽  
Current Distribution ◽  
Current Density ◽  
Electrochemical Cell ◽  
Side Wall ◽  
Cell Geometry ◽  
Electrode Edge ◽  
Side Walls ◽  
In Cells

Anew method for the determination of the ability of an electrolyte to distribute uniformly current density in an electrochemical cell is proposed. It is based on the comparison of the current in cells in which the electrode edges touch the cell side walls with the current in cells with different electrode edge ? cell side wall distances. The effects of cell geometry process parameters and current density are discussed and illustrated using the results presented in the previous papers from this series.

scholarly journals Phosphatidylserine within the Viral Membrane Enhances Chikungunya Virus Infectivity in a Cell-type Dependent Manner

2022 ◽  
Author(s):  
Kerri L Miazgowicz ◽  
Judith Mary Reyes Ballista ◽  
Marissa D Acciani ◽  
Ariana R Jimenez ◽  
Ryan S Belloli ◽  
...  
Keyword(s):  
Chikungunya Virus ◽  
Intervention Strategy ◽  
Host Cells ◽  
Virus Infectivity ◽  
Dependent Manner ◽  
Cell Type ◽  
Enveloped Viruses ◽  
Outer Leaflet ◽  
Viral Particles ◽  
In Cells

Chikungunya virus (CHIKV), an alphavirus of the Togaviridae family, is the causative agent of the human disease chikungunya fever (CHIKF), which is characterized by debilitating acute and chronic arthralgia. No licensed vaccines or antivirals exist for CHIKV. Preventing the attachment of viral particles to host cells is an attractive intervention strategy. Viral entry of enveloped viruses from diverse families including Filoviridae and Flaviviridae is mediated or enhanced by phosphatidylserine receptors (PSRs). PSRs facilitate the attachment of enveloped viruses to cells by binding to exposed phosphatidylserine (PS) in the viral lipid membrane - a process termed viral apoptotic mimicry. To investigate the role of viral apoptotic mimicry during CHIKV infection, we produced viral particles with discrete amounts of exposed PS on the virion envelope by exploiting the cellular distribution of phospholipids at the plasma membrane. We found that CHIKV particles containing high outer leaflet PS (produced in cells lacking flippase activity) were more infectious in Vero cells than particles containing low levels of outer leaflet PS (produced in cells lacking scramblase activity). However, the same viral particles were similarly infectious in NIH3T3 and HAP1 cells, suggesting PS levels can influence infectivity only in cells with high levels of PSRs. Interestingly, PS-dependent CHIKV entry was observed in mosquito Aag2 cells, but not C6/36 cells. These data demonstrate that CHIKV entry via viral apoptotic mimicry is cell-type dependent. Furthermore, viral apoptotic mimicry has a mechanistic basis to influence viral dynamics in vivo in both the human and mosquito host.

scholarly journals Determination of Ca2+ concentration in cells using Ca electrode.

1985 ◽  
Vol 25 (4) ◽  
pp. 168-173 ◽  
Author(s):  
YASUNOBU OKADA
Keyword(s):  
In Cells

scholarly journals A Novel H+ Conductance in Eosinophils

1999 ◽  
Vol 190 (2) ◽  
pp. 183-194 ◽  
Author(s):  
Botond Bánfi ◽  
Jacques Schrenzel ◽  
Oliver Nüsse ◽  
Daniel P. Lew ◽  
Erzsébet Ligeti ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
The Novel ◽  
Experimental Conditions ◽  
Intact Cells ◽  
Electrophysiological Studies ◽  
Dependent Signal ◽  
Low Threshold ◽  
Nadph Oxidase Activation ◽  
In Cells

Efficient mechanisms of H+ ion extrusion are crucial for normal NADPH oxidase function. However, whether the NADPH oxidase—in analogy with mitochondrial cytochromes—has an inherent H+ channel activity remains uncertain: electrophysiological studies did not find altered H+ currents in cells from patients with chronic granulomatous disease (CGD), challenging earlier reports in intact cells. In this study, we describe the presence of two different types of H+ currents in human eosinophils. The “classical” H+ current had properties similar to previously described H+ conductances and was present in CGD cells. In contrast, the “novel” type of H+ current had not been described previously and displayed unique properties: (a) it was absent in cells from gp91- or p47-deficient CGD patients; (b) it was only observed under experimental conditions that allowed NADPH oxidase activation; (c) because of its low threshold of voltage activation, it allowed proton influx and cytosolic acidification; (d) it activated faster and deactivated with slower and distinct kinetics than the classical H+ currents; and (e) it was ∼20-fold more sensitive to Zn2+ and was blocked by the histidine-reactive agent, diethylpyrocarbonate (DEPC). In summary, our results demonstrate that the NADPH oxidase or a closely associated protein provides a novel type of H+ conductance during phagocyte activation. The unique properties of this conductance suggest that its physiological function is not restricted to H+ extrusion and repolarization, but might include depolarization, pH-dependent signal termination, and determination of the phagosomal pH set point.

Determination of the velocity of short electromagnetic waves by interferometry

1952 ◽  
Vol 213 (1112) ◽  
pp. 123-141 ◽  
Keyword(s):  
Phase Velocity ◽  
Electromagnetic Radiation ◽  
Free Space ◽  
Electromagnetic Waves ◽  
Wave Beam ◽  
Wave Length ◽  
Wave Frequency ◽  
Space Wave

A new measurement of the velocity of electromagnetic radiation is described. The result has been obtained, using micro-waves at a frequency of 24005 Mc/s ( λ = 1∙25 cm), with a form of interferometer which enables the free-space wave-length to be evaluated. Since the micro-wave frequency can also be ascertained, phase velocity is calculated from the product of frequency and wave-length. The most important aspect of the experiment is the application to the measured wave-length of a correction which arises from diffraction of the micro-wave beam. This correction is new to interferometry and is discussed in detail. The result obtained for the velocity, reduced to vacuum conditions, is c 0 = 299792∙6 ± 0∙7 km/s.

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