RAPD markers for a sunflower rust resistance gene

1996 ◽  
Vol 47 (3) ◽  
pp. 395 ◽  
Author(s):  
WR Lawson ◽  
KC Goulter ◽  
RJ Henry ◽  
GA Kong ◽  
JK Kochman
Keyword(s):  
Resistance Gene ◽  
Rust Resistance ◽  
Durable Resistance ◽  
Dominant Gene ◽  
Rust Resistance Gene ◽  
Breeding Programs ◽  
Resistant Parent ◽  
F2 Population ◽  
Race 1 ◽  
Selection For

An F2 population of sunflower (Helianthus annuus L.) was tested for segregation of a gene conferring resistance to sunflower rust (Puccznia helzanthi) Australian Race 0 (North American Race 1). The resistant parent, RHA279, of this cross was thought to possess a single dominant resistance gene to this race. Genetic analysis confirmed that this population was segregating for a single dominant gene, the R1 gene, for resistance to this race of the pathogen. Using bulked segregant and RAPD analyses, two markers were identified which co-segregated with the rust resistance gene in the F2 population. These markers, designated OT06959 and 01\/112850, are linked to the rust resistance gene at a distance of 4.5 cM and 26 cM, respectively, with the markers situated one either side of the gene. The availability of markers closely linked to this gene will greatly enhance selection for the gene in future breeding programs, and especially assist efforts to pyramid the gene with other rust resistance genes to produce sunflower varieties with more durable resistance to rust.

BAC-derived markers for assaying the stem rust resistance gene, Sr2, in wheat breeding programs

2008 ◽  
Vol 22 (1) ◽  
pp. 15-24 ◽  
Author(s):  
M. D. McNeil ◽  
R. Kota ◽  
E. Paux ◽  
D. Dunn ◽  
R. McLean ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stem Rust ◽  
Rust Resistance ◽  
Wheat Breeding ◽  
Stem Rust Resistance ◽  
Rust Resistance Gene ◽  
Stem Rust Resistance Gene ◽  
Breeding Programs

scholarly journals Genetic and Molecular Mapping of Stripe Rust Resistance Gene in Wheat–Psathyrostachys huashanica Translocation Line H9020-1-6-8-3

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1482-1487 ◽  
Author(s):  
Qiang Li ◽  
Jing Huang ◽  
Lu Hou ◽  
Pei Liu ◽  
Jinxue Jing ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Durable Resistance ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Translocation Line ◽  
Psathyrostachys Huashanica ◽  
Stripe Rust Resistance Gene

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. The best strategy to control stripe rust is to grow resistant cultivars, but only a few effective genes are available. The wheat accession H9020-1-6-8-3 is a translocation line previously developed from interspecific hybridization between wheat genotype 7182 and Psathyrostachys huashanica, and is resistant to most Chinese Puccinia striiformis f. sp. tritici races. To identify the resistance genes in the translocation line, H9020-1-6-8-3 was crossed with susceptible genotype Mingxian 169, and seedlings of parents and F1, F2, and F3 progenies were tested with prevalent Chinese P. striiformis f. sp. tritici races CYR32 and CYR33 under controlled greenhouse conditions. The genetic results indicated that two single dominant genes in H9020-1-6-8-3 confer resistance to CYR32 and CYR33, respectively. The gene for resistance to CYR33 was temporarily designated as YrH9020. Six simple-sequence repeat markers were used to map the resistance gene to the short arm of wheat chromosome 2D, using 329 F2 plants tested with CYR33 in the greenhouse. The genetic distances of the two closest flanking markers, Xgwm261 and Xgwm455, were 4.4 and 5.8 centimorgans, respectively. Disease assessments and polymorphic tests of the flanking markers among the Psathyrostachys huashanica line and wheat lines 7182, H9020-1-6-8-3, and Mingxian169 suggested that the resistance gene YrH9020 in H9020-1-6-8-3 was originally from P. huashanica. The exotic stripe rust resistance gene and linked molecular markers should be useful for pyramiding with other genes to develop wheat cultivars with high-level and durable resistance to stripe rust.

scholarly journals Inheritance of Resistance to the Peanut Root-knot Nematode in Capsicum chinense

2000 ◽  
Vol 125 (5) ◽  
pp. 615-618
Author(s):  
Richard L. Fery ◽  
Judy A. Thies
Keyword(s):  
Resistance Gene ◽  
Dominant Gene ◽  
Inheritance Of Resistance ◽  
Capsicum Chinense ◽  
Root Knot Nematode ◽  
Sources Of Resistance ◽  
Resistant Parent ◽  
Backcross Populations ◽  
Race 1 ◽  
Allelism Tests

Greenhouse experiments determined the inheritance of resistance to the peanut root-knot nematode [Meloidogyne arenaria (Neal) Chitwood race 1] in Capsicum chinense Jacq. germplasm lines PA-353 and PA-426. Evaluation of parental, F1, F2, and backcross populations of the crosses PA-353 × PA-350 and PA-426 × PA-350 (PA-350 is a susceptible cultigen) indicated that resistance in both C. chinense germplasm lines was conditioned by a single dominant gene. Evaluation of the F1 × resistant parent backcross populations in the cytoplasm of their respective resistant and susceptible parents indicated that the cytoplasm of the resistant parent is not needed for full expression of resistance. Allelism tests indicated that the dominant resistance gene in both PA-353 and PA-426 is allelic to a resistance gene in C. annuum L. `Carolina Cayenne'. However, these allelism tests did not demonstrate conclusively that the M. arenaria race 1 resistance gene in C. chinense is the N gene that conditions resistance to the southern root-knot nematode [Meloidogyne incognita (Kofoid & White) Chitwood] in C. annuum. The ease and reliability of evaluating plants for resistance to root-knot nematodes and the availability of simply inherited sources of resistance makes breeding for peanut root-knot nematode resistance a viable objective in C. chinense breeding programs.

scholarly journals Genetic analysis and molecular mapping of leaf rust resistance genes in the wheat line 5R618

2014 ◽  
Vol 50 (No. 4) ◽  
pp. 262-267 ◽  
Author(s):  
J. Wang ◽  
L. Shi ◽  
L. Zhu ◽  
X. Li ◽  
D. Liu
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Dominant Gene ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

The wheat (Triticum aestivum L.) line 5R618, bred at the China Agricultural University, is resistant in the seedling stage to the majority of the current Chinese pathotypes of wheat leaf rust (Puccinia triticina). To identify and map the leaf rust resistance gene in the 5R618 line, F<sub>2</sub> plants and F<sub>2:3</sub> families from a cross between 5R618 and Zhengzhou5389 (susceptible) were inoculated in the greenhouse with the Chinese P. triticina pathotype THJP. Results from the F<sub>2</sub> and F<sub>2:3</sub> populations indicate that a single dominant gene, temporarily designated&nbsp;Lr5R, conferred resistance. Using the molecular marker method, Lr5R was located on the 3DL chromosome. It was closely linked to the markers Xbarc71 and OPJ-09 with genetic distances of 0.9 cM and 1.0 cM, respectively. At present only one designated gene (Lr24) is located on the 3DL chromosome. The genetic distance between Lr5R&nbsp;and Lr24 confirms that Lr5R is a new leaf rust resistance gene.

scholarly journals Evaluation and Mapping of a Leaf Rust Resistance Gene Derived from Hordeum vulgare subsp. spontaneum

2011 ◽  
Vol 40 (No. 3) ◽  
pp. 86-90 ◽  
Author(s):  
D. Kopahnke ◽  
M. Nachtigal ◽  
Ordon ◽  
F ◽  
J. Steffenson B
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Bulked Segregant Analysis ◽  
Dominant Gene ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Puccinia Hordei ◽  
Doubled Haploid Population ◽  
Leaf Rust Resistance Gene

Studies of marker development were performed on a doubled haploid population derived from the cross of a highly resistant line H. spontaneum 677 &times; Krona (susceptible). Previous segregation studies on F<sub>2</sub> and F<sub>3</sub> populations revealed that the resistance of H. spontaneum 677 was likely due to a single dominant gene. Bulked segregant analysis using AFLPs and SSRs was conducted to identify markers linked to this leaf rust resistance gene. By this approach the resistance gene was located on barley chromosome 2H with the closest markers linked at 6.1 cM (E35M54b) and 13.6 cM (Bmac0218) based on the analysis of 83 DH-lines. In order to get first hints whether this gene may be allelic to rph16 located on chromosome 2H STS marker MWG 2133 co-segregating with rph16 was tested but it turned out to be monomorphic. However, in a resistance test with a set of four different isolates of Puccinia hordei, H. spontaneum 677 showed a different reaction pattern from that of H. spontaneum 680, the source of rph16. Tests of allelism to confirm these results are in progress. &nbsp;

scholarly journals Rust resistance of the French wheat cultivar Renan

2008 ◽  
Vol 43 (No. 2) ◽  
pp. 53-60 ◽  
Author(s):  
A. Hanzalová ◽  
V. Dumalasová ◽  
T. Sumí kova ◽  
P. Bartoš
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Field Experiments ◽  
Dominant Gene ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Adult Plant ◽  
Leaf Rust Resistance Gene ◽  
Aegilops Ventricosa

Our field experiments confirmed the leaf rust resistance of cv. Renan in the Czech Republic. Whereas the leaf rust resistance gene <i>Lr37</i> possessed by Renan is generally effective as late as at the adult plant stage, we found one leaf rust isolate that caused resistant to moderately resistant reactions on NIL <i>Lr37</i> as well as on the cv. Renan already at the seedling stage. This isolate was used in the study of genetics of the leaf rust resistance of cv. Renan in greenhouse experiments. The presence of translocation from <i>Aegilops ventricosa</i> carrying the cluster of rust resistance genes <i>Lr37</i>, <i>Sr38</i> and <i>Yr17</i> was also determined by a PCR molecular marker. All experiments confirmed the presence of <i>Lr37</i> gene in cv. Renan. The presence of <i>Lr14a</i>, postulated earlier, could not be verified. The resistance of cv. Renan in the field was slightly higher than that of the line Tc/8//VPM1 possessing <i>Lr37</i>, which may indicate a more complex genetic base of leaf rust resistance in the cv. Renan. In the progeny of the cross Boka/Renan leaf rust resistance gene <i>Lr37</i> behaved as a recessive or partially dominant gene, stem rust resistance gene <i>Sr38</i> as a dominant gene.

Molecular mapping of stripe rust resistance gene YrH922 in a derivative of wheat (Triticum aestivum)–Psathyrostachys huashanica

2019 ◽  
Vol 70 (11) ◽  
pp. 939
Author(s):  
Zhengwu Fang ◽  
Cai Sun ◽  
Tao Lu ◽  
Zhi Xu ◽  
Wendi Huang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Dominant Gene ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Psathyrostachys Huashanica ◽  
Stripe Rust Resistance Gene

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici Erikss (Pst), is one of the most damaging diseases in common wheat (Triticum aestivum L.) globally. Breeding for genetic resistance is the most effective, economical and ecologically sustainable method to control the disease. The wheat line H922-9-12, developed from a cross between Psathyrostachys huashanica Keng and T. aestivum, was highly resistant to nine Pst races in tests at the seedling stage. To characterise and map the stripe rust resistance gene(s) in H922-9-12, segregating populations were developed by crossing H922-9-12 with the susceptible cultivar Mingxian 169. When tested with Pst race CYR34, the stripe rust resistance in H922-9-12 was shown to be controlled by a single dominant gene, provisionally designated YrH922. A linkage map was constructed with five simple sequence repeat, six expressed sequence tag (EST) and two sequence-related amplified polymorphism markers. YrH922 was located on chromosome 3BL and was 2.7 and 3.4 cM proximal to EST-STS (sequence-tagged site) markers BE517923 and BE471045, respectively. The flanking marker BE517923 in marker-assisted selection for the gene can be used to improve stripe rust resistance on breeding programs.

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