Environmental effects on seed development and hardseededness in Stylosanthes hamata cv. Verano. III. Storage humidity and seed characteristics

1983 ◽  
Vol 34 (3) ◽  
pp. 279 ◽  
Author(s):  
PJ Argel ◽  
LR Humphreys
Keyword(s):  
Relative Humidity ◽  
Temperature Treatment ◽  
Seed Colour ◽  
Seed Moisture Content ◽  
Seed Formation ◽  
Stylosanthes Hamata ◽  
Seed Moisture ◽  
Palisade Cells ◽  
Seed Characteristics ◽  
Seed Provenance

Seed pods (upper articulation) from Stylosanthes hamata cv. Verano plants grown at 27,24 and 21�C from flowering onwards were suspended above saturated salt solutions which provided 77, 32, 15 or 6% relative humidity. The seed moisture content which developed after storage in 77 and 32% relative humidity was negatively related, and the degree of hardseededness positively related, to temperature of seed provenance. Seeds formed under high temperature had more lignin (which was concentrated in the counterpalisade cells) and hemicellulose, less cellulose, and shorter palisade cells than seeds formed under cooler temperature. Cutin content was independent of temperature, but the testa of hard seeds exhibited under the scanning electron microscope a more regular, evenly reticulate surface than that of soft seeds. Seed colour changed from dark to light as temperature of seed formation decreased from 27 to 21�C, but hardseededness within each temperature treatment was not consistently associated with seed colour.

scholarly journals Assessing the storage potential of Australian rainforest seeds: a decision-making key to aid rapid conservation

2021 ◽  
Author(s):  
K. D. Sommerville ◽  
G. Errington ◽  
Z-J. Newby ◽  
G. S. Liyanage ◽  
C. A. Offord
Keyword(s):  
Decision Making ◽  
Logistic Regression ◽  
Dry Weight ◽  
Germination Percentage ◽  
Fleshy Fruit ◽  
Seed Moisture Content ◽  
Fresh Seed ◽  
Desiccation Sensitivity ◽  
Seed Moisture ◽  
Seed Characteristics

AbstractSeed banking of rainforest species is hindered by lack of knowledge as to which species are tolerant of desiccation and freezing. We assessed 313 Australian rainforest species for seed banking suitability by comparing the germination percentage of fresh seeds to seeds dried at 15% RH and seeds stored at −20 °C after drying. We then compared desiccation responses to environmental, habit, fruit and seed characteristics to identify the most useful predictors of desiccation sensitivity. Of 162 species with ≥ 50% initial germination, 22% were sensitive to desiccation, 64% were tolerant and 10% were partially tolerant; the responses of 4% were uncertain. Of 107 desiccation tolerant species tested for response to freezing, 24% were freezing sensitive or short-lived in storage at −20 °C. Median values for fresh seed moisture content (SMC), oven dry weight (DW) and the likelihood of desiccation sensitivity (PD-S) were significantly greater for desiccation sensitive than desiccation tolerant seeds. Ninety-four to 97% of seeds with SMC < 29%, DW < 20 mg or PD-S < 0.01 were desiccation tolerant. Ordinal logistic regression of desiccation response against environmental, habit, fruit and seed characteristics indicated that the likelihood of desiccation sensitivity was significantly increased by a tree habit, fleshy fruit, increasing fresh SMC and increasing PD-S. The responses observed in this study were combined with earlier studies to develop a simple decision key to aid prediction of desiccation responses in untested rainforest species.

scholarly journals Effect of Scarification, Seed Storage Temperature, and Relative Humidity on Lupinus havardii Wats. and Lupinus texensis Hook. Seed Germination

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 782-785 ◽  
Author(s):  
Wayne A. Mackay
Keyword(s):  
Relative Humidity ◽  
Storage Temperature ◽  
Germination Rate ◽  
Seed Storage ◽  
Significant Decline ◽  
Rapid Decline ◽  
Seed Moisture Content ◽  
C Storage ◽  
Seed Moisture ◽  
Seed Scarification

Seeds of Lupinus havardii Wats. and L. texensis Hook. were subjected to scarification, storage temperature (4 or 22 °C), and relative humidity (RH) treatments (11%, 23%, 52%, 75%, or 97% RH) for 12 months. Seed moisture increased as relative humidity increased with scarified seed having the greatest increase in seed moisture content regardless of storage temperature. For both species, the combination of seed scarification before storage, 75% RH, and 22 °C storage temperature resulted in a significant and rapid decline in germinability beginning at 4 months. Scarified L. texensis seed stored at 52% RH and 22 °C also exhibited a significant decline in germinability following 6 months storage. Seed of both species stored under all other conditions germinated similar to or higher than the initial germination rate after 12 months. These results clearly show that scarification can be performed before seed packaging as long as the seed packets are stored at ≤23% RH under 4 or 22 °C with no loss in germinability for at least 1 year.

scholarly journals Role of Relative Humidity in Processing and Storage of Seeds and Assessment of Variability in Storage Behaviour inBrassicaspp. andEruca sativa

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
A. Suma ◽  
Kalyani Sreenivasan ◽  
A. K. Singh ◽  
J. Radhamani
Keyword(s):  
Relative Humidity ◽  
Seed Viability ◽  
Seed Storage ◽  
Eruca Sativa ◽  
Seed Moisture Content ◽  
Seedling Vigour ◽  
Seed Moisture ◽  
Processing And Storage ◽  
And Storage

The role of relative humidity (RH) while processing and storing seeds ofBrassicaspp. andEruca sativawas investigated by creating different levels of relative humidity, namely, 75%, 50%, 32%, and 11% using different saturated salt solutions and 1% RH using concentrated sulphuric acid. The variability in seed storage behaviour of different species ofBrassicawas also evaluated. The samples were stored at40±2°Cin sealed containers and various physiological parameters were assessed at different intervals up to three months. The seed viability and seedling vigour parameters were considerably reduced in all accessions at high relative humidity irrespective of the species. Storage at intermediate relative humidities caused minimal decline in viability. All the accessions performed better at relative humidity level of 32% maintaining seed moisture content of 3%. On analyzing the variability in storage behaviour,B. rapaandB. junceawere better performers thanB. napusandEruca sativa.

scholarly journals Prediction of cottonseed longevity

2006 ◽  
Vol 41 (9) ◽  
pp. 1435-1441 ◽  
Author(s):  
Roberto Usberti ◽  
Eric Hywel Roberts ◽  
Richard Harold Ellis
Keyword(s):  
Moisture Content ◽  
Storage Period ◽  
Temperature Treatment ◽  
Survival Curves ◽  
Initial Value ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Moisture Content Level ◽  
Equilibrium Relative Humidity ◽  
Storage Temperatures

The objective of this work was to determine the viability equation constants for cottonseed and to detect the occurrence and depletion of hardseededness. Three seedlots of Brazilian cultivars IAC-19 and IAC-20 were tested, using 12 moisture content levels, ranging from 2.2 to 21.7% and three storage temperatures, 40, 50 and 65ºC. Seed moisture content level was reached from the initial value (around 8.8%) either by rehydration, in a closed container, or by drying in desiccators containing silica gel, both at 20ºC. Twelve seed subsamples for each moisture content/temperature treatment were sealed in laminated aluminium-foil packets and stored in incubators at those temperatures, until complete survival curves were obtained. Seed equilibrium relative humidity was recorded. Hardseededness was detected at moisture content levels below 6% and its releasing was achieved either naturally, during storage period, or artificially through seed coat removal. The viability equation quantified the response of seed longevity to storage environment well with K E = 9.240, C W = 5.190, C H = 0.03965 and C Q = 0.000426. The lower limit estimated for application of this equation at 65ºC was 3.6% moisture content.

scholarly journals Evaluation of Temperature and Moisture Content during Storage on the Germination of Flowering Annual Seed

HortScience ◽  
1995 ◽  
Vol 30 (5) ◽  
pp. 1003-1006 ◽  
Author(s):  
William J. Carpenter ◽  
Eric R. Ostmark ◽  
John A. Cornell
Keyword(s):  
Relative Humidity ◽  
Moisture Content ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Seed Moisture ◽  
Storage Temperatures

Various combinations of temperature and moisture contents were used in evaluating the seed storage of nine genera of annual flowers. Relative humidity (RH) levels of 11%, 32%, 52%, and 75% provided wide ranges in seed moisture during storage at 5, 15, and 25C. At each temperature, total germination percentages (G) generally declined as seed moisture content increased during storage. The seed moisture range giving the highest G after 12 months of storage was determined for each temperature and plant genus. For all genera, seed moisture contents during storage increased as storage temperatures increased at constant RH levels. Moisture contents at 25C storage were 37%, 34%, 29%, and 20% higher than at 5C when RH levels were at 11%, 32%, 52%, and 75%, respectively.

scholarly journals Conservation of Garcinia imberti Bourd. through seeds

2019 ◽  
Vol 6 (2) ◽  
pp. 243-251
Author(s):  
M Anto ◽  
M Angala ◽  
P S Jothish ◽  
C Anilkumar
Keyword(s):  
Seed Germination ◽  
Relative Humidity ◽  
Moisture Content ◽  
Gibberellic Acid ◽  
Biosphere Reserve ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Evergreen Forests ◽  
Seed Moisture ◽  
Subsequent Exposure

Garcinia imberti seeds were collected during 2015-2017 from Shangili, Cheenikkala and Bonaccord evergreen forests of Agasthyamala Biosphere Reserve, the only abode of this endangered endemic species. Germinability of seeds were analysed through decoating, Gibberellic acid (GA3) and light inductive pre-treatments on fresh (62.8 % moisture content; MC) and desiccated (fast; 23.3% MC and slow; 30.5 % MC) seeds. The seed germination with impermeable coat (0.7-1.2 mm) was restricted which on decoating got enhanced. Application of GA3 along with exposure to light breaked dormancy within 4-6 days compared to non-treated seeds that took 238-254 days to germinate. Stored seeds behaviour revealed that seed moisture content and rate of germination were negatively correlated. Seed storage was found to be more efficient only up to 80 days at controlled seed banking conditions (20 ± 20C, 20 % relative humidity; RH). Both fast and slow desiccated seeds stored for 60 days in seed bank conditions exhibited 50.4 and 43.4 % of germination compared 39.4% germination of non-desiccated seeds. Hence fast desiccated and decoated G. imberti seeds pre-treated with GA3 on subsequent exposure to light alleviated dormancy. For seed banking, fast desiccated seeds with MC in between 40-20% are found to be promising.

scholarly journals Three-dimensional Models Represent Seed Moisture Content as a Function of Relative Humidity and Temperature

HortScience ◽  
1998 ◽  
Vol 33 (7) ◽  
pp. 1207-1209 ◽  
Author(s):  
Jian Fang ◽  
Frank Moore ◽  
Eric Roos ◽  
Christina Walters
Keyword(s):  
Relative Humidity ◽  
Moisture Content ◽  
Citrullus Lanatus ◽  
Three Dimensional ◽  
Selection Criterion ◽  
Seed Storage ◽  
Response Surfaces ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Three Dimensional Models

Seed moisture content (MC) has been considered the most important factor controlling physiological reactions in seeds, and MC changes with relative humidity (RH) and temperature (T). This relationship is revealed by studying the interaction of RH and T at equilibrium. Cucumber (Cucumis sativus L.), lettuce (Lactuca sativa L.), maize (Zea mays L.), onion (Allium cepa L.), pea (Pisum sativum L.), and watermelon (Citrullus lanatus M. & N.) seeds were equilibrated over sulfuric acid (1% RH) and various saturated salt solutions (5.5% to 93% RH) at temperatures from 5 to 50 °C. Best-fit subset models were selected from the complete third-order model MC = β0 + β1*RH + β2*T + β3*RH2 + β4*T2 + β5*RH*T + β6*RH3 + β7*T3 + β8*RH*T2 + β9*RH2*T, using Mallows' minimum Cp as the selection criterion. All six best subset models (R2, 0.98 to 0.99) had the same functional form, MC = β0 + β1*RH + β2*T + β3*RH2 + β5*RH*T + β6*RH3 + β9*RH2*T. Coefficients had essentially the same respective values among all species except onion and pea, for which some coefficients were statistically different from those of the other species (P ≤ 0.05). All models indicated that seed MC increased as RH increased and decreased as T increased; but RH had the greater influence. The inverse relationship between seed MC and T, although slight, was evident in the response surfaces. The interaction effect of RH and T on MC was significant at P ≤0.001. These results suggest that orthodox seed species respond similarly to T and RH. This in turn suggests that a common model could be developed and used for optimizing seed storage environments.

scholarly journals Effect of relative humidity, initial seed moisture content and storage container on soybean (Glycine max L. Meril.) seed quality

2015 ◽  
Vol 39 (3) ◽  
pp. 461-469
Author(s):  
MR Ali ◽  
MM Rahman ◽  
KU Ahammad
Keyword(s):  
Relative Humidity ◽  
Moisture Content ◽  
Dry Matter ◽  
Soybean Seed ◽  
Germination Percentage ◽  
Seed Moisture Content ◽  
Storage Container ◽  
Seed Moisture ◽  
Storage Containers ◽  
Initial Seed

To find out the effect of storage relative humidity, seed moisture content and type of storage container on soybean seed quality, an experiment was conducted at the Seed Laboratory, Department of Agronomy, Bangladesh Agricultural University Mymensingh in 2008 and 2009. In 2008, soybean seed has 96% initial germination and in 2009 seed having 98% initial germination was stored at 8% and 12% initial moisture levels in two types of storage containers viz., cloth bag and polythene bag (0.06mm thickness). The final seed moisture content, germination percentage, germination index, and seedling dry matter of the seed under different treatments were measured at 60,120, and 180 days after storage (DAS). The experiment was arranged in a completely randomized design with three replications. In 2008, highest germination percentage (89.33%) of soybean seed was retained at 180 days after storage (DAS) for those stored at 8% initial seed moisture content (SMC) in polythene bag at 50% relative humidity. Germination index and seedling dry matter decreased with increased initial seed moisture content irrespective of storage containers used. In 2009, highest germination percentage (92.67%) of soybean seed was retained at 180 DAS for those stored in polythene bag at 8% initial SMC at 50% of the relative humidity. Those stored in cloth bag at 12% SMC showed rapid germination loss and the value went down to 0.00 in both the years. Vigour index and seedling dry matter decreased with increased initial seed moisture content irrespective of storage containers used. DOI: http://dx.doi.org/10.3329/bjar.v39i3.21989 Bangladesh J. Agril. Res. 39(3): 461-469, September 2014

Environmental effects on seed development and hardseededness in Stylosanthes hamata cv. Verano. II. Moisture supply and illuminance

1983 ◽  
Vol 34 (3) ◽  
pp. 271 ◽  
Author(s):  
PJ Argel ◽  
LR Humphreys
Keyword(s):  
Seed Production ◽  
Day Treatment ◽  
Seedling Emergence ◽  
Field Capacity ◽  
Seed Setting ◽  
Negative Effects ◽  
Seed Moisture Content ◽  
Seed Formation ◽  
Stylosanthes Hamata ◽  
Moisture Supply

The effects of variation in moisture supply and of level of illuminance during seed formation on the development of hardseededness and on the seed production of Stylosanthes hamata cv. Verano were studied in two glasshouse experiments. Verano plants were grown in pots containing 1.6 kg soil and were watered daily to field capacity until early flowering (43 days after seedling emergence). From day 43 to day 116, the plants were watered every 1, 2, 3 or 4 days. Leaf water potential immediately before rewatering varied from c. -20 to -28 bars in the 4-day treatment. Yields of seed pods were 5.8, 4.6, 3.8 and 3.8 g in the I-, 2-, 3- and 4-day treatments respectively. Severe water stress greatly reduced inflorescence density, had lesser negative effects on frequency of blooming, floret number, seed setting and proportion of florets forming a lower (beakless) articulation, and increased individual pod weight. Watering treatment had transitory and inconsistent effects on hardseededness. Seed production of Verano plants grown after flowering under shades with 30,40 or 59 % transmission or no shade was reduced by low level of illuminance, mainly because of effects on floret number per inflorescence; the proportion of florets forming a lower articulation and individual pod weight (but not seed setting) was also reduced by shading. The seed moisture content at different harvest occasions appeared to influence hardseededness, but shading had no consistent effect.

Environmental effects on seed development and hardseededness in Stylosanthes hamata cv. Verano. I. Temperature

1983 ◽  
Vol 34 (3) ◽  
pp. 261 ◽  
Author(s):  
PJ Argel ◽  
LR Humphreys
Keyword(s):  
Seed Development ◽  
Environmental Effects ◽  
Storage Conditions ◽  
Seed Setting ◽  
Negative Effects ◽  
Seed Formation ◽  
Moisture Contents ◽  
Stylosanthes Hamata ◽  
Seed Moisture ◽  
Embryo Dormancy

The level of hardseededness in Stylosanthes hamata cv. Verano grown in the open at St Lucia was positively and linearly related to temperature during seed formation; this relationship was stronger in 1976 than in 1977 when warmer conditions occurred. In two other experiments Verano plants were grown under the same conditions until early flowering and then exposed to differing temperature regimens. Seed production was maximal at 31/24�C (day/night), and cool temperatures (20/16�C or 18�C constant) had negative effects on inflorescence differentiation, rate of floret blooming and seed setting. Hardseededness developed most strongly at the high temperatures (35/28, 31/24�C, or 27 and 24�C constant), and this was also allied with lower seed moisture contents at harvest and in varying storage conditions. These findings may explain the variation in hardseededness of seed lots of differing provenance, which also may bear on the climatic adaptation of this short-lived plant. A range of mechanisms promote continued seed dormancy and its progressive release. Verano exhibited a transitory and weakly developed embryo dormancy at maturity, strong pod and testa inhibition of germination which decreased after 120 days of storage, and hardseededness continuing after 180 days of storage; the last was greater in the upper than in the lower articulation or seed pod.

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