A selective medium for determining the population of viable conidia of Cochliobolus sativus in soil

1982 ◽  
Vol 33 (2) ◽  
pp. 287 ◽  
Author(s):  
RL Dodman ◽  
JR Reinke
Keyword(s):  
Rose Bengal ◽  
Complete Recovery ◽  
Selective Medium ◽  
Cochliobolus Sativus ◽  
Ecological Studies ◽  
Dilution Plate ◽  
Different Soils

A selective medium containing three antibiotics, three fungicides, and rose Bengal has been developed for the determination of populations of viable conidia of Cochliobolus sativus in soils by the dilution plate procedure. With this medium, complete recovery of added conidia was achieved; the level of recovery was not significantly different (P > 0.05) when conidia were present in two different soils. The medium should be useful for ecological studies of C. sativus.

Fourth Order Derivative Spectrophotometric Determination of Lead with 1,10-Phenanthroline and Rose Bengal

1993 ◽  
Vol 26 (3) ◽  
pp. 523-539 ◽  
Author(s):  
D. Sreevalsan Nair ◽  
T. Prasada Rao ◽  
C. S. P. Iyer ◽  
A. D. Damodaran
Keyword(s):  
Spectrophotometric Determination ◽  
Fourth Order ◽  
Rose Bengal ◽  
Order Derivative

Erythrocyte porphyrin analysis in the detection of lead poisoning in children: evaluation of four micromethods.

1976 ◽  
Vol 22 (2) ◽  
pp. 161-168 ◽  
Author(s):  
T L Hanna ◽  
D N Dietzler ◽  
C H Smith ◽  
S Gupta ◽  
H S Zarkowsky
Keyword(s):  
Acetic Acid ◽  
Ethyl Acetate ◽  
Lead Poisoning ◽  
Complete Recovery ◽  
Comparison Method ◽  
Working Standard ◽  
Single Extraction ◽  
Double Extraction ◽  
Poisoning In Children

Abstract We evaluated four procedures for determination of erythrocyte porphyrin: double extraction with ethyl acetate/acetic acid-HCl, single extraction with ethanol, single extraction with acetone, and direct solubilization with detergent-buffer. The ethyl acetate procedure, when used with two portions of HCl, apparently gives complete recovery of porphyrin and is suitable for reference as a comparison method. The ethanol procedure gives a high and consistent recovery and is technically simpler. The acetone procedure gives low and variable recovery of porphyrin, and the detergent-buffer method is subject to serious hemoglobin interference; neither of these two procedures offers any technical advantage. Stability of samples and methods for standardization were explored. A procedure for expressing results in terms of erythrocyte Zn-protoporphyrin content is given. Because of its stability, coproporphyrin is useful as a daily working standard. The ethyl acetate and ethanol methods are about equally efficient for detecting lead intoxication. Because of its simplicity, the ethanol method seems to be the best for use in screening.

scholarly journals Properties, processes and regimes of soil solutions and surface waters

2020 ◽  
Vol 175 ◽  
pp. 12022
Author(s):  
Guriyat Podvolotskaya ◽  
Sergey Belopukhov ◽  
Vitaly Savich ◽  
Andrey Sorokin ◽  
Nikolay Tyutrin
Keyword(s):  
Biological Activity ◽  
Surface Water ◽  
Surface Waters ◽  
Pair Correlation ◽  
Rate Of Change ◽  
Water Soluble ◽  
Soil Solutions ◽  
Different Depths ◽  
Different Soils

Soil solutions and the surface waters are characterized by properties, processes and regimes. Soil solutions of different soils and their surface water have different biological activity and change the activity of dissolved stimulants and inhibitors. The object of the study are soil solutions of the main types of soils obtained in the model experiments with the ratio of soils: water equal to 1:1 and 1: 2, soil solutions and surface water in the flooding of soils with water for 1 week – 3 months. The research method consisted in the assessment of pH, Eh, activity of K, NO3, NH4, Ca, Mg by conventional methods, assessment of concentrations of water-soluble compounds extracted from soils by ionite membranes, in the assessment of biological activity of solutions using biotests. The following is suggested for additional evaluation: the using of cation and anion membranes, determination of interrelation between the properties of waters, equation of pair correlation and multiply regression. The informative value of the gradient of surface water concentrations at different distances from the floor of the reservoir, at different depths of the overwatered soils is shown. The mobility of Ca, Mg, Fe, Mn in soils and the content of their water-soluble forms depends on both pH and Eh, whose influence on the content of water-soluble forms of the considered cations shows the effects of synergy and antagonism. The rate of change in the composition of soil solutions during soil flooding depends on a combination of soil properties, temperature, and duration of flooding. Soil solutions of different soils and their surface waters have differentrates.

Isolation of Potential Alkaliphilic Cellulase-Producer Strains from Limestone Area of Perlis

2015 ◽  
Vol 754-755 ◽  
pp. 1054-1058
Author(s):  
Nor’izzah Zainuddin ◽  
Khadijah Hanim Abd Rahman ◽  
Abdul Razak Shaari ◽  
Siti Jamilah Hanim Mohd Yusof
Keyword(s):  
Selective Medium ◽  
Plate Method ◽  
Dilution Plate ◽  
Cellulolytic Fungi ◽  
The Future ◽  
Alkaline Cellulase ◽  
Dilution Plate Method ◽  
Soil Dilution ◽  
Positive Results ◽  
Producer Strains

Alkaliphilic cellulases-producer fungi were isolated from soil of limestone areas in Perlis. The potential strain was isolated by soil dilution plate method on enriched selective medium using CMC as substrate at different pH levels. Eleven isolates of different morphological colonies were screened using the hydrolysis capacity test by Gram’s iodine. Out of eleven colonies, five showed positive results as the hydrolysis zone formed. BK1 showed the highest hydrolysis capacity among all sample strains for every pH levels. Eventually, four strains were selected to be further explored as cellulolytic fungi for the production of alkaline cellulase in the future.

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