Prediction of body composition in live sheep

1974 ◽  
Vol 25 (5) ◽  
pp. 825 ◽  
Author(s):  
JR Donnelly ◽  
M Freer

New and published data on the chemical composition of 149 Merino and Merino crossbred sheep covering a wide range of age were used to derive regressions of body water, protein, ash and fat on parameters measured in the live animal. Allometric models were tested with fasted liveweight (W), tritiated water space (T) and maturity (M) as predictors, maturity being the age of the sheep as a proportion of the age at which mature size is reached. For all body components the inclusion of maturity in the following equations substantially reduced the residual standard deviation (RSD), compared with allometric or multiple linear regression models that included only W and T as predictors. In addition it largely eliminated bias from the predicted values for the six separate groups of sheep from which the data were obtained. Total body water (kg) = 1.179T0.926M0.031 (RSD = ?0.354) Protein in empty body (kg) = 0.408 W0.708M0.092 (RSD = ? 0.264) Ash in empty body (kg) = 0.305 W0.460M0.290 (RSD = ? 0.100) Fat in empty body (kg) = 0.117(W – T)1.592M-0.225 (RSD = ? 0.444) It is suggested that in future work a measure of skeletal size in the live animal may increase the precision of these equations.

1982 ◽  
Vol 33 (2) ◽  
pp. 375 ◽  
Author(s):  
PR Bird ◽  
PC Flinn ◽  
JWD Cayley ◽  
MJ Watson

The body composition of 23 Hereford steers was related to their liveweight after a 24 h fast (W), total body water (TBW), tritiated water space (T) and age in months (A) by using allometric or linear regression models. Fifteen steers, of similar initial age, were taken at intervals from a grazing experiment having a wide range of stocking rates. These steers were 15-22 months of age and 189-461 kg W at slaughter. Six younger and two older steers were also used to expand the range to 3-33 months and 90-517 kg. The steers were taken from pasture 3 h after sunrise and deprived of feed and water thereafter. After 4 h, tritiated water was given intramuscularly and 20 h later blood was collected and the animals were weighed and slaughtered. Models having the least residual standard deviation (r.s.d.) and the predictors which contributed significantly in the stepwise regression analysis (P i 0.05) are given for each range of W. T greatly improved the estimation of fat and slightly improved the prediction of protein. A only slightly improved the prediction of TBW and fat. Equations applicable to the narrow range of W were: total body water (kg) =


1976 ◽  
Vol 54 (6) ◽  
pp. 857-862 ◽  
Author(s):  
R. D. Cameron ◽  
R. G. White ◽  
J. R. Luick

The accuracy of the tritium water dilution method in estimating water flux was evaluated in reindeer under various conditions of temperature and diet. Two non-pregnant female reindeer were restrained in metabolism stalls, within controlled-environment chambers, at temperatures of + 10, −5, and −20 °C; varying amounts of a commercial pelleted ration (crude protein, 13%) or mixed lichens (crude protein, 3%) were offered, and water was provided ad libitum either as snow or in liquid form. Total body water volume and water turnover were estimated using tritiated water, and the daily outputs of feces and urine were measured for each of 12 different combinations of diet and temperature. Statistical analysis of the data showed that the tritium water dilution technique gives accurate determinations of total body water flux over a wide range of environmental and nutritional conditions.


1998 ◽  
Vol 25 (5) ◽  
pp. 499 ◽  
Author(s):  
K. L. Viggers ◽  
D. B. Lindenmayer ◽  
R. B. Cunningham ◽  
C. F. Donnelly

Two methods of assessing body condition were examined for the mountain brushtail possum, Trichosurus caninus (Ogilby) at a single study site in central Victoria, south-eastern Australia. Condition indices were derived from measures of skeletal size and body mass, and body water composition was determined using isotopic dilution of tritiated water to give an estimate of total body water space. There were significant negative relationships between body water composition and the condition indices for body length, total length and pes length. Body water composition of T. caninus was significantly higher in spring than in winter, and those animals with higher body water composition had lower absolute neutrophil counts and higher urea values. The relative usefulness of the two methods used for estimating body condition in T. caninus is discussed.


1979 ◽  
Vol 92 (1) ◽  
pp. 69-81 ◽  
Author(s):  
J. Z. Foot ◽  
E. Skedd ◽  
D. N. McFarlane

SummaryIn two experiments with female Scottish Blackface or Border Leicester x Scottish Blackface sheep nine or ten animals were slaughtered in mid lactation and the remainder either in November at the time of the subsequent mating (Expt 1) or at weaning (Expt 2).The sheep were infused periodically, including just before slaughter, with 100 μCi tritiated water (TOH) in order to measure total body water by dilution and to estimate body fat using the inverse relationship between the proportions of fat and water in the body. The accuracy of the methods was assessed when the sheep were slaughtered. In the ton lactating Scottish Blackface sheep of Expt 1 fat made up 11.5% of the total body weight with an S.D. of 8·38% whereas the 11 sheep slaughtered at mating were twice as fat (23·2, S.D. 4·01 %). The 25 Border Leicester × Blackface sheep were all thin, whether they were slaughtered in mid lactation (4·4, S.D. 2·56%) or at weaning (3·4, S.D. 2·81%).The standard error of estimate of body water from TOH space in Expt 2 was 1·2 kg c.v. 2·8%) and lower in Expt 1.The precision with which an animal could be weighed was very important in determining the accuracy with which body fat could be predicted from live weight and TOH space. In both experiments the standard error of estimate for body fat in lactating sheep was between 600 and 700 g, compared with 1·3–2·7 kg when body fat was predicted from live weight alone. These estimates were sufficiently accurate to be of value in following changes in body composition in live animals as their nutritional and physiological state alters and for comparing animals in groups where the average fatness is greater and the range wider than in the sheep used in Expt 2.


1969 ◽  
Vol 72 (1) ◽  
pp. 31-40 ◽  
Author(s):  
W. R. McManus ◽  
R. K. Prichard ◽  
Carolyn Baker ◽  
M. V. Petruchenia

SUMMARYThe use of tritiated water to estimate total body-water content of animals experiencing recovery from under-nutrition was studied.The time for equilibration of tritiated water (TOH), given intraperitoneally, with total body water (TBW) was determined in rabbits and in rats. As judged by the specific activity of blood water, equilibration had occurred by 76–125 min in the rabbit and did not appear to be affected by the plane of nutrition. However, between slaughter groups the specific activity of water obtained from the liver 180 min after injection of TOH was significantly different from the specific activity of water simultaneously obtained from the blood plasma. It is concluded that the liver is not a suitable tissue to use for testing achievement of equilibration.As judged by the specific activity of blood water compared to that of water from the whole body macerate, equilibration in mature rats either in stable body condition or undergoing rapid compensatory growth occurred in less than 60 min.A trial comparing TOH-space (corrected by 3% body weight) and actual TBW (by desiccation) was conducted on thirty rabbits which experienced under-nutrition followed by compensatory growth.Prior to under-nutrition the agreement between actual and estimated TBW was satisfactory and within 2·3%. During compensatory growth the agreement was poor— the TOH values over-estimating actual TBW by about 12%.A trial with mature rats confirmed the findings with rabbits. For rats in stable body weight the mean estimated TOH-space for fourteen animals was within 1·2% of the actual TBW. For fourteen rats undergoing compensatory growth the mean estimated TOH-space (corrected by 3% body weight) overestimated actual TBW by 6·2%.


Author(s):  
Vasileios T. Stavrou ◽  
Kyriaki Astara ◽  
Zoe Daniil ◽  
Konstantinos I. Gourgoulianis ◽  
Konstantinos Kalabakas ◽  
...  

The purpose of the study is to investigate whether the oxygen uptake and heart rate at rest, in Greek professional soccer players, are affected by recent injuries, as well as how sleep quality is affected. Forty-two male professional soccer players were included in the study and divided into two groups: injurygroup (n = 22, age: 21.6 ± 5.4 years, body fat: 11.0 ± 3.9%, total body water: 64.0 ± 2.5%) and no-injurygroup (n = 20, age: 24.2 ± 5.6 years, body fat: 10.1 ± 2.8%, total body water: 64.3 ± 1.8%). The oxygen uptake at rest (VO2resting, mL/min/kg) and heart rate (HR, bpm) were recorded in the upright position for 3 min, and the predicted values were calculated. One hour before, the athletes answered the Pittsburgh Sleep Quality Index (PSQI) questionnaire. The results showed a difference between groups (injurygroup vs. no-injurygroup) in VO2resting (7.5 ± 1.4 vs. 5.5 ± 1.2 mL/min/kg, p < 0.001) and percent of predicted values (92.5 ± 17.2 vs. 68.3 ± 14.6%, p < 0.001) and HR, such as beats per min (100.6 ± 12.8 vs. 93.1 ± 4.6 bpm, p = 0.001), percent of predicted values (50.7 ± 6.4 vs. 47.6 ± 2.8%, p = 0.003) and sleep quality score (PSQI: 4.9 ± 2.2 vs. 3.1 ± 0.9, p = 0.005). Anthropometric characteristics were not different between groups. Oxygen consumption and heart rate at rest are affected by the systemic adaptations due to injury. These pathophysiological changes probably relate to increased blood flow in an attempt to restore the injury area.


1963 ◽  
Vol 14 (6) ◽  
pp. 926 ◽  
Author(s):  
BA Panaretto ◽  
AR Till

The antipyrine, tritiated water, and N-acetyl-4-aminoantipyrine spaces were determined simultaneously in goats which had been deprived of feed and water for 48 hr. The animals were then killed, minced, and analysed for water, fat, protein, and ash contents. The compositions of the whole and empty bodies of the goats were calculated, and the relationships between the bodily components were compared with those reported for cattle, sheep, and some monogastric species. The relationships found between the components of the whole bodies compared favourably with those derived from the empty bodies. The relationships of the spaces determined in vivo to total body water, fat, and protein were found, and confidence statements were placed on predicted estimates.


1988 ◽  
Vol 59 (1) ◽  
pp. 109-124 ◽  
Author(s):  
R. J. Johnson ◽  
D. J. Farrell

1. Birds (n169) which varied in age, live weight, nutritional history, physiological state and genotype were slaughtered and analysed for total body water. Before slaughter, birds were injected with the water isotopes tritiated water (TOH) or deuterium oxide (D2O), or both, to determine TOH space or D2O space, or both, as estimates of total body water in vivo.2. At the mean total body water of all birds determined by desiccation, of 1096·4 (SD 424·1) g, TOH space and D2O space overestimated total body water by 10·4 and 8·5 % respectively. The difference between the isotopes was significant (P< 0·05).3. Based on recovery of isotope it was postulated that the main reason for the observed overestimation of total body water in vivo was incomplete recovery of isotope due to the vacuum sublimation technique. The mean recovery (%) of added isotope to whole blood after vacuum sublimation was 93·0 (SD 2·6) and 92·4 (SD 5·5) of the theoretical concentrations of TOH and D2O respectively.4. Nevertheless, accurate prediction of total body water was obtained from regression equations which included live weight and isotope-dilution space. Values required logarithmic (base 10) transformation before derivation of linear and multiple linear regression equations, and the precision of prediction was determined by the residual standard deviation (RSD).5. Total body water could be predicted with nearly equal accuracy from live weight or isotope-dilution space (RSD 0·025 and 0·020 respectively). Prediction of carcass protein was more accurate from live weight (RSD 0·033) than from TOH space (RSD 0·036), and inclusion of both variables resulted in only a marginal decrease in RSD to 0·031.6. The prediction of carcass fat and energy was markedly improved by the inclusion of isotope-dilution space in conjunction with live weight compared with live weight alone.7. The relations show the developmental nature of body composition of domestic fowl given diets adequate in nutrients. The prediction equations demonstrate the precision possible for studies in which estimates of body composition in poultry are required without slaughter.


1983 ◽  
Vol 101 (2) ◽  
pp. 257-264 ◽  
Author(s):  
P. R. N. Chigaru ◽  
D. H. Holness

SUMMARYThe body composition of 18 each of Mashona, Afrikaner and Hereford heifers was measured at the beginning and after 16 and 32 weeks of the experiment. The heifers not slaughtered at the beginning of the experiment were fed a complete diet containing 132 g crude protein and 12·0 MJ metabolizable energy/kg dry matter. Before slaughter, the animals were deprived of food and water for 24 h. Each animal was infused with 1 mCi of tritiated water (TOH) in order to measure total body water (TBW) and to estimate body fat.The growth rate of the three breeds of heifers was similar despite differences in age and initial live weight. Both TBW and fat proportions, however, differed significantly (P < 0·01) between slaughter stages for each breed and between breeds at each slaughter stage. At the first, second and final slaughter stages the proportions of TBW were: 68·0, 59·4 and 54·5% for Mashona; 70·;5, 64·3 and 58·3% for Afrikaner and 65·3, 57·6 and 46·2% for Hereford heifers respectively. The corresponding proportions of body fat were: 10·2, 18·4 and 24·2% for Mashona; 6·6, 12·0 and 20·0% for Afrikaner and 13·7, 20·8 and 25·8% for Hereford heifers respectively.There was a close relation between empty body weight and live weight at slaughter which was not influenced by breed. Both TBW and fat were estimated more accurately when TOH space and live weight were used jointly. However, the slopes of the prediction equations for each breed were significantly different (P < 0·05) in the case of both total body water and fat. It was necessary to use separate equations for each breed in order to predict either body water or fat. The significance of these findings for the estimation of body fat in live cattle is discussed.


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