The occurrence of bacterial leaf blight in wild and cultivated rice in northern Australia

1973 ◽  
Vol 24 (2) ◽  
pp. 219 ◽  
Author(s):  
SJ Aldrick ◽  
IW Buddenhagen ◽  
APK Reddy
Keyword(s):  
Distinct Pattern ◽  
Leaf Blight ◽  
Oryza Rufipogon ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Northern Australia ◽  
Asian Countries ◽  
Cultivated Rice ◽  
Wild Rice Species ◽  
First Time

Bacterial leaf blight (Xanthomonas oryzae) of rice is reported for the first time in Australia, where it occurred on cultivated rice and also on the indigenous wild rice species Oryza rufipogon and O. australiensis in the Northern Territory. The pathogen was isolated, characterized, and tested in Hawaii on a differential set of rice cultivars. All Australian isolates of X. oryzae had a lower average virulence, and a distinct pattern of virulence, in comparison with about 200 isolates from 10 Asian countries. The Australian isolates may represent a separate pathotype of X. oryzae, which suggests that the pathogen is indigenous and not recently introduced into northern Australia.

scholarly journals Phenotype and genotype variability of interspecific rice lines related to bacterial leaf blight resistance (Xanthomonas oryzae pv. oryzae) character

2021 ◽  
Vol 22 (10) ◽  
Author(s):  
SITI YURIYAH ◽  
DEDY DARNAEDI ◽  
TATANG MITRA SETIA ◽  
GUT WINDARSIH ◽  
Dwinita Wikan Utami
Keyword(s):  
Resistance Gene ◽  
Wild Rice ◽  
Leaf Blight ◽  
Oryza Rufipogon ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Yield Potential ◽  
Interspecific Crosses ◽  
Blight Resistance ◽  
Wild Rice Species

Abstract. Yuriyah S, Darnaedi D, Setia TM, Windarsih G, Utami DW. 2021. Phenotype and genotype variability of interspecific rice lines related to bacterial leaf blight resistance (Xanthomonas oryzae pv. oryzae) character. Biodiversitas 22: 4123-4130. Wild rice species are the source of the gene pool for rice genetic diversity. The cross-species crosses (interspecific crosses) play an important role in breeding, namely in terms of expanding the diversity of desirable characters, such as disease resistance and improvement in yield potential. Currently, the genes from wild rice species have been successfully introgressed into cultivated rice so that they can overcome the rice production constraints, including the introgression of genes for the bacterial leaf blight (BLB) resistance. The purpose of this study was to analyze the variability of phenotype and genotype performance of lines derived from wild rice species for the character of resistance to BLB disease. A total of 33 selected backcross lines derived from wild rice species of O. rufipogon and O. glaberrima as the donor parents and 2 control varieties (resistant and susceptible to BLB disease), were used in this study. The evaluation of phenotype and genotype of resistance characters to BLB disease was carried out during a vegetative phase of the plant. The resistance evaluation was carried out in a greenhouse, while the genotype performance was analyzed using 4 STS markers linked to Xa4, Xa7, and Xa13 genes. The result of the phenotype evaluation showed that there were variations among the resistance of the tested lines. Three lines derived from a Situ Bagendit/Oryza rufipogon cross were resistant to all BLB races used in the testing. Those three lines indicated to have the allele of the Xa7 resistance gene based on the genotype performance which grouped with the Code variety that had the Xa7 resistance gene.

scholarly journals Molecular Detection of Resistance To Bacterial Leaf Blight on Conde Indonesian Rice Variety

2018 ◽  
Vol 22 (1) ◽  
pp. 27 ◽  
Author(s):  
Fatimah Fatimah ◽  
Joko Prasetiyono ◽  
Aqwin Polosoro ◽  
Mushlihatun Baroya
Keyword(s):  
Sequence Alignment ◽  
Molecular Detection ◽  
Nucleotide Substitution ◽  
Gene Action ◽  
Rice Variety ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Cultivated Rice ◽  
Similar Gene

Rice bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo) affected grain yield and decreasing rice production in rice growing countries. Conde, Indonesian rice variety, exhibits high resistance to most of the Indonesian races of (BLB) and has been used in Indonesia for cultivated rice. This study was aimed to conduct the molecular detection between proximal markers in chromosome 6 and relative expression of Conde rice variety compare to IRBB7 in Xa7 region. The population screening, BLB evaluation and molecular detection around the Xa7 region were conducted. The results showed from the collection of individual recombinants between resistant and susceptible parents narrow the region containing the BTBPOZ domain. The sequence alignment of Xa7LD37 in two resistant and three susceptible cultivars demonstrated a perfect association. The sequence alignment in exon region of Loc_Os06g46240 in Nipponbare, IRBB7, and IR64 identified indel/SNPs in this region leading to nucleotide substitution and frameshift resulting in amino acid change between resistant and susceptible cultivars. It was predicted that Conde revealed the similar gene action with Xa7 gene for BLB that encodes a BTB POZ domain.

scholarly journals Molecular Detection of Resistance To Bacterial Leaf Blight on Conde Indonesian Rice Variety

2018 ◽  
Vol 22 (1) ◽  
pp. 27
Author(s):  
Fatimah Fatimah ◽  
Joko Prasetiyono ◽  
Aqwin Polosoro ◽  
Mushlihatun Baroya
Keyword(s):  
Sequence Alignment ◽  
Molecular Detection ◽  
Nucleotide Substitution ◽  
Gene Action ◽  
Rice Variety ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Cultivated Rice ◽  
Similar Gene

Rice bacterial leaf blight (BLB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo) affected grain yield and decreasing rice production in rice growing countries. Conde, Indonesian rice variety, exhibits high resistance to most of the Indonesian races of (BLB) and has been used in Indonesia for cultivated rice. This study was aimed to conduct the molecular detection between proximal markers in chromosome 6 and relative expression of Conde rice variety compare to IRBB7 in Xa7 region. The population screening, BLB evaluation and molecular detection around the Xa7 region were conducted. The results showed from the collection of individual recombinants between resistant and susceptible parents narrow the region containing the BTBPOZ domain. The sequence alignment of Xa7LD37 in two resistant and three susceptible cultivars demonstrated a perfect association. The sequence alignment in exon region of Loc_Os06g46240 in Nipponbare, IRBB7, and IR64 identified indel/SNPs in this region leading to nucleotide substitution and frameshift resulting in amino acid change between resistant and susceptible cultivars. It was predicted that Conde revealed the similar gene action with Xa7 gene for BLB that encodes a BTB POZ domain.

scholarly journals Uji Ketahanan Galur-galur Harapan Padi terhadap Penyakit Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae) Ras III, IV, dan VIII

2016 ◽  
Vol 19 (2) ◽  
pp. 53
Author(s):  
Siti Yuriyah ◽  
Dwinita W. Utami ◽  
Ida Hanarida
Keyword(s):  
Molecular Biology ◽  
Leaf Blight ◽  
Oryza Rufipogon ◽  
Xanthomonas Oryzae ◽  
Resistance Test ◽  
Bacterial Leaf Blight ◽  
Rice Varieties ◽  
Rice Germplasm ◽  
Pure Cultures ◽  
Different Levels

<p>Resistance Test of Promising Rice Lines Against Bacterial Leaf Blight (Xanthomonas oryzae pv. oryzae) Race III, IV, and VIII. Siti Yuriyah, Dwinita W. Utami, and Ida Hanarida. Development of new superior rice varieties resistant to bacterial leaf blight (BLB) has been conducted through utilizing of a wide rice germplasm, from crossing between IR64 and Oryza rufipogon. The aim of this study is to get promising rice lines that resistant to BLB race III, IV, and VIII. The experiments were conducted at greenhouse and Laboratory of Molecular Biology, ICABIOGRAD Bogor, using of 13 promising rice lines that have different levels of resistance to inoculum from pure cultures of BLB race III, IV and VIII. Of these 13 rice lines, six lines showed resistance to race III (Bio5-AC-Blas/BLB-03, Bio62-AC-Blas/BLB-03, Bio111-BC-PIR7, Bio129-BC-WBC, Bio148-Mamol, and Bio154-Mamol-Dro), one line showed resistance to race IV (Bio154-Mamol-Dro), and one line showed resistance to race VIII (Bio5-AC-Blas/BLB-03), with severity rate 1.8 to 8.1%. Of these improve lines Bio5-AC-Blas/BLB-3 and Bio 111-BCPir- 7, were released as new rice varieties, namely Inpari HDB and Inpari Blas, respectively.</p><p> </p><p><strong>Abstrak</strong></p><p>Perakitan varietas padi unggul baru tahan terhadap hawar daun bakteri (HDB) terus dilakukan melalui pemanfaatan sumber daya genetik yang luas. Saat ini telah dirakit galur-galur unggul yang berasal dari persilangan IR64 dan padi liar Rupifogon. Penelitian ini bertujuan untuk mengevaluasi ketahanan galur harapan padi terhadap penyakit HDB dari Ras III, IV, dan VIII. Percobaan dilakukan di rumah kaca dan Laboratorium Kelti Biologi Molekuler, BB Biogen, Bogor. Materi genetik yang digunakan adalah 13 galur harapan padi dan biakan murni dari Ras III, IV, dan VIII. Metode inokulasi dengan pengguntingan dan skoring ketahanan dilakukan sesuai dengan sistem penilaian dari Yashitola et al. (1997). Dari 13 galur padi harapan yang diuji, diperoleh enam galur tahan terhadap Ras III (Bio5-AC-Blas/BLB-03, Bio62-AC-Blas/BLB-03, Bio111- BC-PIR7, Bio129-BC-WBC, Bio148-Mamol-Dro, dan Bio154-Mamol-Dro), satu galur tahan terhadap Ras IV (Bio154-Mamol-Dro) dan satu galur tahan terhadap Ras VIII (Bio5-AC-Blas/BLB-03), dengan tingkat keparahan sebesar 1,8-8,1%. Dalam perkembangannya, dua dari galur harapan, yaitu galur Bio5-AC-Blas/BLB-03 dan Bio111-BC-Pir-7 disetujui untuk dilepas sebagai varietas Inpari HDB dan Inpari Blas.</p>

scholarly journals Response of a local rice variety Pandanwangi to infection of bacterial leaf blight Xanthomonas oryzae pv. oryzae strain III, IV, and VIII

2021 ◽  
Vol 905 (1) ◽  
pp. 012068
Author(s):  
Hadiwiyono ◽  
S H Poromarto ◽  
S Widono ◽  
R F Rizal
Keyword(s):  
Rice Variety ◽  
Leaf Blight ◽  
Rice Production ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Artificial Inoculation ◽  
Limiting Factors ◽  
Bacterial Suspension ◽  
West Java ◽  
Local Cultivar

Abstract Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the limiting factors in rice production. A local cultivar, rice “Pandanwangi” is a superior variety much preferred and cultivated by the farmers in Cianjur, West Java, Indonesia. The information about the response of “Pandangwangi” to Xoo is still poorly understood. This paper reports the results of the evaluation of “Pandanwangi” response against BLB. This research was conducted in a greenhouse with artificial inoculation using Xoo strains III, IV, and VIII with bacterial suspension at 108 cfu.mL−1. The results showed that the response of cv Pandanwangi to Xoo infection was different from the strain of Xoo. “Pandanwangi” cultivar was susceptible to Xoo strain III and VIII and very susceptible to strain IV.

scholarly journals Discovery of Novel Dihydrolipoamide S-Succinyltransferase Inhibitors Based on Fragment Virtual Screening

2021 ◽  
Vol 22 (23) ◽  
pp. 12953
Author(s):  
Chengqian Wei ◽  
Junjie Huang ◽  
Yu Wang ◽  
Yifang Chen ◽  
Xin Luo ◽  
...  
Keyword(s):  
Virtual Screening ◽  
Antibacterial Agents ◽  
High Efficiency ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Extracellular Polysaccharides ◽  
Bacterial Leaf Streak ◽  
Bacterial Diseases ◽  
Sulfone Derivatives

A series of new oxadiazole sulfone derivatives containing an amide moiety was synthesized based on fragment virtual screening to screen high-efficiency antibacterial agents for rice bacterial diseases. All target compounds showed greater bactericidal activity than commercial bactericides. 3-(4-fluorophenyl)-N-((5-(methylsulfonyl)-1,3,4-oxadiazol-2-yl)methyl)acrylamide (10) showed excellent antibacterial activity against Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola, with EC50 values of 0.36 and 0.53 mg/L, respectively, which were superior to thiodiazole copper (113.38 and 131.54 mg/L) and bismerthiazol (83.07 and 105.90 mg/L). The protective activity of compound 10 against rice bacterial leaf blight and rice bacterial leaf streak was 43.2% and 53.6%, respectively, which was superior to that of JHXJZ (34.1% and 26.4%) and thiodiazole copper (33.0% and 30.2%). The curative activity of compound 10 against rice bacterial leaf blight and rice bacterial leaf streak was 44.5% and 51.7%, respectively, which was superior to that of JHXJZ (32.6% and 24.4%) and thiodiazole copper (27.1% and 28.6%). Moreover, compound 10 might inhibit the growth of Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola by affecting the extracellular polysaccharides, destroying cell membranes, and inhibiting the enzyme activity of dihydrolipoamide S-succinyltransferase.

Riboflavin (Vitamin B2) mediated defence induction against bacterial leaf blight: probing through chlorophyll a fluorescence induction O–J–I–P transients

2018 ◽  
Vol 45 (12) ◽  
pp. 1251 ◽  
Author(s):  
Shasmita ◽  
Harekrushna Swain ◽  
Anuprita Ray ◽  
Pradipta K. Mohapatra ◽  
Ramani K. Sarkar ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Oryza Sativa L ◽  
Bacterial Pathogen ◽  
Fluorescence Induction ◽  
Leaf Blight ◽  
Xanthomonas Oryzae ◽  
Bacterial Leaf Blight ◽  
Yield Data ◽  
Progress Curve ◽  
Moderately Resistant

Bacterial leaf blight (BLB) is a serious threat for rice (Oryza sativa L.) cultivation caused by the bacterial pathogen Xanthomonas oryzae pv. oryzae. The pathogen mainly damages the leaf chlorophyllous tissue, resulting in poor photosynthesis and causing up to 50% reductions in grain yield. In the present work, we have compared the structural and functional ability of the chloroplast of three varieties of rice with different degrees of susceptibility (TN1, highly susceptible; IR-20, moderately resistant; DV-85, resistant to BLB) treated with riboflavin (1 and 2 mM) and infected with BLB, with chlorophyll fluorescence as a tool. As indicated by the chlorophyll fluorescence technique, the disease progress curve and yield data, riboflavin acted as an effective vitamin for inducing resistance against BLB. Plants treated with riboflavin showed improved PSII activity, more chlorophyll content and higher yield than the diseased plants.

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