Ovulation rate and survival of fertilized ova in Merino ewes selected for and against multiple births

1972 ◽  
Vol 23 (5) ◽  
pp. 851 ◽  
Author(s):  
AO Trounson ◽  
NW Moore
Keyword(s):  
Ovulation Rate ◽  
The Other ◽  
Multiple Births ◽  
Corpora Lutea ◽  
Significant Difference ◽  
The Mean ◽  
Selection Group ◽  
Pregnant Mare Serum Gonadotrophin

Peppin Merino ewes selected for (T group) and against (0 group) multiple births over a number of generations were used in an experiment designed to study the effect of selection on ovulation rate, response to exogenous gonadotrophin (1300 i.u. pregnant mare serum gonadotrophin—PMSG), and their ability to support one or more embryos of their own or the other group. Fertilized eggs, collected following treatment of donors with PMSG, were transferred within and between groups at a rate of one and three per recipient. Recipients were not treated with PMSG. The mean ovulation rates of T and 0 recipients were 2.03 and 1.13, and the mean ovulatory response to PMSG of T ewes was almost 50 % greater than that of O ewes (4.76 v. 3.29 corpora lutea). The survival to birth of transferred eggs was affected by both the selection group of the egg and the selection group of the recipient, and by the number of eggs transferred. More O than T recipients lambed (77 % v. 45 %); and within O recipients, intergroup transfers were more successful than intragroup transfers (94 % v. 54% ewes lambed). A similar but not significant difference was observed in T group recipients (54% v. 38% lambed). Where three eggs were transferred, neither the selection group of the recipient nor that of the egg had any effect upon the incidence of multiple births. It is concluded that selection has operated primarily on ovulation rate, and there was some evidence of heterosis in intergroup transfers.

Increased production of sheep embryos following superovulation of Merino ewes with a combination of pregnant mare serum gonadotrophin and follicle stimulating hormone

1991 ◽  
Vol 3 (5) ◽  
pp. 551 ◽  
Author(s):  
JP Ryan ◽  
JR Hunton ◽  
WM Maxwell
Keyword(s):  
Follicle Stimulating Hormone ◽  
Ovarian Response ◽  
Ovulation Rate ◽  
Corpora Lutea ◽  
Embryo Recovery ◽  
Designed Experiment ◽  
The Mean ◽  
Mean Concentration ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulating Hormone

In a factorially designed experiment (N = 321), 0, 800 or 1600 I.U. pregnant mare serum gonadotrophin (PMSG) were administered in combination with 0, 12 or 18 mg follicle stimulating hormone (FSH-P) to superovulate Merino ewes in autumn and spring. A moderate dose of PMSG (800 I.U.) in conjunction with 12 or 18 mg FSH-P increased the ovulation rate above that observed when FSH-P was used alone. This was accomplished by (i) increasing the proportion of ewes that exhibited a superovulatory response (greater than 3 corpora lutea (CL) or persistent large follicles (LF): 69/70 (99%) v. 55/74 (74%), P less than 0.001), and (ii) in those ewes that exhibited a superovulatory response, by an additive effect of exogenous gonadotrophin (14.8 +/- 0.9 CL (69) v. 11.3 +/- 0.9 CL (55), P less than 0.01) without increasing the incidence of LF. The use of 1600 I.U. PMSG in conjunction with 12 or 18 mg FSH-P was characterized by an increase in the number of LF and, in comparison with 800 I.U. PMSG, a reduction in ovulation rate. Season had no effect on the numbers of CL, but total ovarian response (CL + LF) was higher in autumn than in spring (P less than 0.01), because of a greater incidence of LF (P less than 0.001). The proportion of ewes with regressed CL was higher in autumn than in spring (53/143 (37%) v. 32/156 (21%), P less than 0.01), and increased with increased dose of gonadotrophin. Furthermore, a nutritional component to the incidence of ewes with regressed CL was suggested by the observation that the mean concentration of plasma glucose was higher for ewes with normal CL than for ewes with regressed CL (P less than 0.05). Rates of ova or embryo recovery, fertilization and embryo development generally declined with an increase in the incidence of LF as a result of increases in the dose of gonadotrophin and season of administration.

Multi-Center Study of Thromboplastin Calibration Precision – Influence of Reagent Species, Composition, and International Sensitivity Index (ISI)

1993 ◽  
Vol 69 (01) ◽  
pp. 035-040 ◽  
Author(s):  
A M H P van den Besselaar ◽  
R M Bertina
Keyword(s):  
Oral Anticoagulants ◽  
International Normalized Ratio ◽  
The Other ◽  
World Health ◽  
Rabbit Brain ◽  
Sensitivity Index ◽  
Bovine Plasma ◽  
Significant Difference ◽  
The Mean ◽  
International Sensitivity Index

SummaryFour thromboplastin reagents were tested by 18 laboratories in Europe, North-America, and Australasia, according to a detailed protocol. One thromboplastin was the International Reference Preparation for ox brain thromboplastin combined with adsorbed bovine plasma (coded OBT/79), and the second was a certified reference material for rabbit brain thromboplastin, plain (coded CRM 149R). The other two thromboplastin reagents were another rabbit plain brain thromboplastin (RP) with a lower ISI than CRM 149R and a rabbit brain thromboplastin combined with adsorbed bovine plasma (RC). Calibration of the latter two reagents was performed according to methods recommended by the World Health Organization (W. H. O.).The purpose of this study was to answer the following questions: 1) Is the calibration of the RC reagent more precise against the bovine/combined (OBT/79) than against the rabbit/plain reagent (CRM 149R)? 2) Is the precision of calibration influenced by the magnitude of the International Sensitivity Index (ISI)?The lowest inter-laboratory variation of ISI was observed in the calibration of the rabbit/plain reagent (RP) against the other rabbit/plain reagent (CRM 149R) (CV 1.6%). The highest interlaboratory variation was obtained in the calibration of rabbit/plain (RP) against bovine/combined (OBT/79) (CV 5.1%). In the calibration of the rabbit/combined (RC) reagent, there was no difference in precision between OBT/79 (CV 4.3%) and CRM 149R (CV 4.2%). Furthermore, there was no significant difference in the precision of the ISI of RC obtained with CRM 149R (ISI = 1.343) and the rabbit/plain (RP) reagent with ISI = 1.14. In conclusion, the calibration of RC could be performed with similar precision with either OBT/79 or CRM 149R, or RP.The mean ISI values calculated with OBT/79 and CRM 149R were practically identical, indicating that there is no bias in the ISI of these reference preparations and that these reference preparations have been stable since their original calibration studies in 1979 and 1987, respectively.International Normalized Ratio (INR) equivalents were calculated for a lyophilized control plasma derived from patients treated with oral anticoagulants. There were small but significant differences in the mean INR equivalents between the bovine and rabbit thromboplastins. There were no differences in the interlaboratory variation of the INR equivalents, when the four thromboplastins were compared.

scholarly journals Corneal Biomechanical Parameters and Central Corneal Thickness in Glaucoma Patients, Glaucoma Suspects, and a Healthy Population

2021 ◽  
Vol 10 (12) ◽  
pp. 2637
Author(s):  
Mª. Ángeles del Buey-Sayas ◽  
Elena Lanchares-Sancho ◽  
Pilar Campins-Falcó ◽  
María Dolores Pinazo-Durán ◽  
Cristina Peris-Martínez
Keyword(s):  
Central Corneal Thickness ◽  
Corneal Thickness ◽  
Mean Difference ◽  
The Other ◽  
Control Group ◽  
Healthy Population ◽  
Diagnostic Categories ◽  
Significant Difference ◽  
Biomechanical Parameters ◽  
The Mean

Purpose: To evaluate and compare corneal hysteresis (CH), corneal resistance factor (CRF), and central corneal thickness (CCT), measurements were taken between a healthy population (controls), patients diagnosed with glaucoma (DG), and glaucoma suspect patients due to ocular hypertension (OHT), family history of glaucoma (FHG), or glaucoma-like optic discs (GLD). Additionally, Goldmann-correlated intraocular pressure (IOPg) and corneal-compensated IOP (IOPcc) were compared between the different groups of patients. Methods: In this prospective analytical-observational study, a total of 1065 patients (one eye of each) were recruited to undergo Ocular Response Analyzer (ORA) testing, ultrasound pachymetry, and clinical examination. Corneal biomechanical parameters (CH, CRF), CCT, IOPg, and IOPcc were measured in the control group (n = 574) and the other groups: DG (n = 147), FHG (n = 78), GLD (n = 90), and OHT (n = 176). We performed a variance analysis (ANOVA) for all the dependent variables according to the different diagnostic categories with multiple comparisons to identify the differences between the diagnostic categories, deeming p < 0.05 as statistically significant. Results: The mean CH in the DG group (9.69 mmHg) was significantly lower compared to controls (10.75 mmHg; mean difference 1.05, p < 0.001), FHG (10.70 mmHg; mean difference 1.00, p < 0.05), GLD (10.63 mmHg; mean difference 0.93, p < 0.05) and OHT (10.54 mmHg; mean difference 0.84, p < 0.05). No glaucoma suspects (FHG, GLD, OHT groups) presented significant differences between themselves and the control group (p = 1.00). No statistically significant differences were found in the mean CRF between DG (11.18 mmHg) and the control group (10.75 mmHg; mean difference 0.42, p = 0.40). The FHG and OHT groups showed significantly higher mean CRF values (12.32 and 12.41 mmHg, respectively) than the DG group (11.18 mmHg), with mean differences of 1.13 (p < 0.05) and 1.22 (p < 0.001), respectively. No statistically significant differences were found in CCT in the analysis between DG (562 μ) and the other groups (control = 556 μ, FHG = 576 μ, GLD = 569 μ, OHT = 570 μ). The means of IOPg and IOPcc values were higher in the DG patient and suspect groups than in the control group, with statistically significant differences in all groups (p < 0.001). Conclusion: This study presents corneal biomechanical values (CH, CRF), CCT, IOPg, and IOPcc for diagnosed glaucoma patients, three suspected glaucoma groups, and a healthy population, using the ORA. Mean CH values were markedly lower in the DG group (diagnosed with glaucoma damage) compared to the other groups. No significant difference was found in CCT between the DG and control groups. Unexpectedly, CRF showed higher values in all groups than in the control group, but the difference was only statistically significant in the suspect groups (FHG, GLD, and OHT), not in the DG group.

Comparison of plasma progesterone profiles in cyclic, pregnant, pseudopregnant and hysterectomized pigs between 8 and 27 days after oestrus

1988 ◽  
Vol 119 (1) ◽  
pp. 111-116 ◽  
Author(s):  
G. J. King ◽  
R. Rajamahendran
Keyword(s):  
Least Squares ◽  
Analysis Of Variance ◽  
The Other ◽  
Corpora Lutea ◽  
Plasma Progesterone ◽  
The Third ◽  
Secretory Potential ◽  
The Mean

ABSTRACT Plasma progesterone concentrations were compared in cyclic (n = 12), pregnant (n =12), oestradiol-induced pseudopregnant (n=12) and hysterectomized gilts (n=10) between days 8 and 27 after oestrus. The results were grouped into periods covering days 8–13, 14–20 and 21–27 and analysed by least-squares analysis of variance. Plasma progesterone concentrations were significantly (P<0·001) higher in hysterectomized compared with other groups between days 8 and 13. Progesterone concentrations declined rapidly after day 14 in cyclic females and gradually in the other groups. Throughout the third and fourth weeks the mean progesterone concentrations for hysterectomized animals were consistently higher than for pseudopregnant animals (P<0·05). The pregnant group means were below but not significantly different from the hysterectomized means in both of the last two periods. The greater progesterone concentrations in hysterectomized gilts indicated that secretion is high without any conceptus-produced or -mediated luteotrophin, and corpora lutea in cyclic, pregnant or pseudopregnant gilts may never reach full secretory potential. J. Endocr. (1988) 119, 111–116

Effect of grain or silage supplementation on meat quality attributes of cryptorchid lambs grazing lucerne or annual grass-clover pastures

1995 ◽  
Vol 35 (4) ◽  
pp. 461 ◽  
Author(s):  
DL Hopkins ◽  
PJ Holst ◽  
DG Hall
Keyword(s):  
Meat Quality ◽  
Shear Force ◽  
The Other ◽  
Carcass Weight ◽  
Annual Grass ◽  
Eye Muscle ◽  
Mean Values ◽  
Significant Difference ◽  
The Mean ◽  
Ad Libitum

Objective and subjective tests for quality were performed on the meat from 40 cryptorchid second-cross lambs fed lucerne only (L; n = 8), lucerne plus an oat grain supplement ad libitum (LO; n = lo), lucerne plus wilted lucerne silage ad libitum (LS; n = 11), or an oat-lupin grain (3:1) supplement at 90% of ad libitum while grazing dry summer annual pasture (OL; n = 11). All carcasses complied with the Elite lamb specification of weight >22 kg and GR measurement 6-15 mm. There was no significant difference between groups for hot carcass weight, the mean (� s.e) values being 25.1 0.43, 25.2 � 0.36,25.2 � 0.38, and 25.3 � 0.36 kg for L, LS, LO, and OL groups, respectively. When the GR and fat depth over the eye muscle at the 12th rib were adjusted to a common carcass weight of 25.2 kg there was no significant difference between groups. There was no significant difference between groups for pH, or the colour values a*, b*, and L* of the M. longissimus thoracis et lumborum (a* is relative redness, b* relative yellowness, and L* relative lightness). The shear force values for the loin muscle were significantly (P<0.05) greater for lambs from group OL than for those from group LO. Cooking loss for the loin muscle was significantly (P<0.05) greater for OL lambs than all other groups. A comparison of the mean values for aroma and flavour showed the lambs from group OL produced meat that was considered significantly (P<0.05) less desirable for these characteristics, with other groups being similar. Meat from OL-fed lambs was considered oilier and less meaty than meat from lambs fed the other diets. There was a significant (P<0.05) difference in absolute scores between panellists, but their ranking was not affected and there was no panellist x sample interaction. Because many producers use grain supplements such as oats and lupins for finishing lambs on dry forages, further study is recommended that focuses on the interaction between supplement and the base forage.

Vaccination against follistatin and the effect on ovulation rate in estrus-induced gilts

2003 ◽  
Vol 83 (3) ◽  
pp. 487-492
Author(s):  
C. R. Christensen ◽  
M. J. Redmond ◽  
B. Laarveld
Keyword(s):  
Treatment Group ◽  
Key Words ◽  
Luteal Phase ◽  
Ovulation Rate ◽  
Corpora Lutea ◽  
Significant Difference ◽  
Ovarian Morphology ◽  
Antibody Titers

Primiparous sows were vaccinated against follistatin to determine the effect on ovulation rate following typical commercial estrus induction and synchronization. Seventy-five gilts received four vaccinations against a recombinant porcine follistatin (FS) or a sham vaccine (CTL). At 85 kg, gilts were induced into estrus with a combination of PG600 and hCG and synchronized using PGF2α. At the second estrus, antibody titers ranged from 0 to1:6400 in the FS-vaccinated treatment group and no FS antibodies were detected in the CTL group. Late in the second subsequent luteal phase the reproductive tracts of the gilts that had displayed two estruses were collected. There was no significant difference in the number of corpora lutea (FS = 13.2 ± 0.5, CTL = 14.5 ± 0.7) or corpora albicantia (FS = 12.1 ± 1.9, CTL = 12.3 ± 2.0) between treatments. Follistatin-vaccinated gilts displayed an increased number of luteal structures which resembled corpora hemorrhagica (P = 0.04). This study shows that vaccination of gilts against FS concurrent with estrus induction and synchronization affected ovarian morphology, although an effect on ovulation rate was not apparent. Key words: Swine, follistatin, immunoneutralization, fecundity, ovulation rate

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

scholarly journals Influence of toothbrushing on enamel softening and abrasive wear of eroded bovine enamel: an in situ study

2006 ◽  
Vol 20 (2) ◽  
pp. 148-154 ◽  
Author(s):  
Daniela Rios ◽  
Heitor Marques Honório ◽  
Ana Carolina Magalhães ◽  
Marília Afonso Rabelo Buzalaf ◽  
Regina Guenka Palma-Dibb ◽  
...  
Keyword(s):  
Abrasive Wear ◽  
The Other ◽  
In Situ Study ◽  
Significant Difference ◽  
The Mean ◽  
Student’S T ◽  
Bovine Enamel ◽  
Enamel Layer ◽  
Student’S T Test

This study assessed the surface softening and abrasive wear of eroded bovine enamel with or without the influence of toothbrushing. Five volunteers took part in this in situ study of 5 days. They wore acrylic palatal appliances containing 6 bovine enamel blocks divided in two rows with 3 blocks, which corresponded to the studied groups: erosion without toothbrushing (GI) and erosion with toothbrushing (GII). The blocks were subjected to erosion by immersion of the appliances in a cola drink for 10 minutes, 4 times a day. After that, no treatment was performed in one row (GI), whereas the other row was brushed (GII). The appliance was then replaced into the mouth. Enamel alterations were determined using profilometry and microhardness tests. Data were tested using paired Student’s t test (p < 0.05). The mean wear values (µm) and percentage of superficial microhardness change (%SMHC) were respectively: GI - 2.77 ± 1.21/91.61 ± 3.68 and GII - 3.80 ± 0.91/58.77 ± 11.47. There was a significant difference in wear (p = 0.001) and %SMHC (p = 0.001) between the groups. It was concluded that the wear was more pronounced when associated to toothbrushing abrasion. However, toothbrushing promoted less %SMHC due to the removal of the altered superficial enamel layer.

scholarly journals A-31 Trails-X Trail-Level Performance Using the Profile Variability Index

2019 ◽  
Vol 34 (6) ◽  
pp. 890-890
Author(s):  
J Greene ◽  
M Messer ◽  
D Hartman ◽  
C Reynolds
Keyword(s):  
The Other ◽  
Hyperactivity Disorder ◽  
Standardization Sample ◽  
Significant Difference ◽  
Trail Making ◽  
New Type ◽  
Variability Index ◽  
The Mean ◽  
Low Performance ◽  
Level Performance

Abstract Objective The Trails-X is a new type of trail-making test that emphasizes executive function and does not require either literacy or numeracy. The purpose of this study was to examine performance variability across trails. Method Participants were the Trails-X standardization sample and individuals with traumatic brain injury (TBI), learning disabilities (LD), intellectual disabilities (ID), attention-deficit hyperactivity disorder (ADHD), and dementia. Trail-level performance was assessed via the mean Matrix, Connected Circles, and Time to Discontinue scores across trails. The Profile Variability Index (PVI) was calculated (Plake, Reynolds, & Gutkin, 1981) and clinical groups and standardization samples were compared via a one-way ANOVA. Results Across all trails, the mean Matrix score was 6 (possible range = 1-12) for the standardization sample and as low as 3 for the dementia and ID samples. The mean Connected Circles score was 16 (possible range = 0-22) and as low as 12 for the dementia sample. The mean Time to Discontinue score was 39 seconds (possible range = 1-75) and as high as 55 for the dementia sample. There was a statistically significant difference on the PVI score (F(5,845) = 6.921, p = .000). The dementia (M = 4.77) and ID (M = 5.11) samples had significantly lower (p < .05) PVI scores than the other samples, which were not significantly different from each other. Conclusions The dementia and ID samples were characterized by consistently low performance while the other samples were characterized by moderate amounts of variability, indicating that some variability across trails should be expected within less impaired individuals.

scholarly journals Ovarian characteristics in sheep with multiple fecundity genes

Reproduction ◽  
2017 ◽  
Vol 153 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Kenneth P McNatty ◽  
Derek A Heath ◽  
Zaramasina Clark ◽  
Karen Reader ◽  
Jennifer L Juengel ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Total Mass ◽  
Total Cell ◽  
Ovulation Rate ◽  
Corpora Lutea ◽  
Preovulatory Follicles ◽  
Luteal Tissue ◽  
In The Wild ◽  
The Mean ◽  
The One

Ewes heterozygous for combinations of the Inverdale (FecXI; I+), Booroola (FecB; B+) and Woodlands (FecX2W; W+) mutations have ovulation rates higher than each mutation separately. The aims of the experiments described herein were to examine the ovarian phenotypes in I+B+ and I+B+W+ ewes and to compare these with the appropriate ++ (controls), I+ and BB animals available for this study. The mean ± s.e.m. ovulation rates in the ++ (n = 23), I+ (10), I+B+ (7), I+B+W+ (10) and BB (3) animals were 1.8 ± 0.1, 2.5 ± 0.2, 6.6 ± 1.0, 9.6 ± 0.9 and 9.7 ± 0.9 respectively. The maximum number of granulosa cells per follicle in the ++ and I+ genotypes was accumulated after exceeding 5 mm diameter, whereas in I+B+, I+B+W+ and BB animals, this was achieved when follicles reached >2–3 mm. The number of putative preovulatory follicles, as assessed from those with LH-responsive granulosa cells, 24 h after the induction of luteolysis, was higher (P < 0.01) in the I+B+ and I+B+W+ compared to the ++ and I+ genotypes. The median follicular diameters of these follicles in the ++, I+, I+B+, I+B+W+ and BB genotypes were 6, 5, 3, 3 and 3 mm respectively. The total number of granulosa cells in the putative preovulatory follicles when added together, and total mass of luteal tissue, did not differ between the genotypes. Thus, despite large ovulation rate differences between animals with one or more fecundity genes, the total cell compositions over all preovulatory follicles and corpora lutea, when added together, are similar to that from the one or two such follicles in the wild types.

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