Studies on the artificial insemination of Merino sheep. III. The effect of frequent ejaculation on semen characteristics and fertilizing capacity

1962 ◽  
Vol 13 (6) ◽  
pp. 1137 ◽  
Author(s):  
S Salamon
Keyword(s):  
Linear Relationship ◽  
Artificial Insemination ◽  
Volume Density ◽  
Merino Sheep ◽  
Significant Linear Relationship ◽  
Normal Sperm ◽  
Abnormal Sperm ◽  
Semen Characteristics ◽  
Linear Decline ◽  
Lambing Rate

(1) Fifty-five ejaculates were collected from each of two Merino rams over a period of 5 days (11 per day per ram at intervals of 20 min). Ejaculates were examined for semen characteristics and for fertilizing capacity following artificial insemination, a total of 300 ewes being used. (2) Volume, density, and number of sperm declined on successive days, and in successive ejaculates within days (P <0.001). Motility and percentage of abnormal sperm were not affected by frequent collection. (3) Fertility following insemination with a standard volume of diluted semen showed a significant linear decline with successive ejaculates, both within and between days of collection ( P <0.01, <0.001). (4) The decline in fertility could be accounted for entirely on the basis of sperm numbers. There was no evidence of a decline in the fertilizing capacity of spermatozoa with frequent ejaculation. (5) There was a highly significant linear relationship between sperm numbers and lambing percentage, over the range of 28 to 128 X 106 sperm (27–118 x 106 normal; b = 0.27; r = 0.69; P <0.01). (6) It is concluded that a dose of approximately 120–125 x l06 normal sperm is necessary for maximum fertility to artificial insemination; and that each 25 x 106 reduction down to a dose of 25 x 106 may be associated with a drop in lambing rate of about 13%.

scholarly journals Comparison of semen characteristics between indigenous and Amo breeds cockerel of Gombe State, Nigeria

2019 ◽  
Vol 15 (2-1) ◽  
pp. 303-306
Author(s):  
Tijjani Haruna Usman ◽  
Saleh Mohammed Sir ◽  
Ma’aruf Bashir Sani
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Indigenous Breed ◽  
Normal Sperm ◽  
Abnormal Sperm ◽  
Significant Difference ◽  
Semen Characteristics ◽  
Genetically Improved ◽  
Abdominal Massage

The experiment was carried out to compare the semen characteristics of indigenous and Amo strains of cockerel at poultry unit of teaching and research farm of Federal University of Kashere, Gombe State, Nigeria. Semen samples were collected from nine indigenous and nine Amo breeds of cockerel at three days interval for two weeks using abdominal massage technique. Semen samples were examined macroscopically for semen colour, pH and ejaculation volume. Then, microscopic observation was carried for sperm concentration, mass motility, progressive motility, live and dead sperms percentage, normal and abnormal sperm, all for semen characteristics. The results showed a significant difference (P ≤ 0.05) between mass motility, progressive motility, sperm concentration and head defects of 4.85 ± 0.27 to 4.37 ± 0.19, 95.13 ± 0.43 to 81.63 ± 1.15%, 4.93 ± 1.84 to 3.40 ± 1.07×109/ml and 2.96 ± 0.17 to 3.44 ± 0.12% for indigenous and Amo breeds of cockerel, respectively. There were no significant differences observed as semen colour, ejaculate volume, semen pH, live / dead normal sperm neck (mid-piece), tail defects and sperm total abnormalities were found to be 2.85 ± 0.07 to 2.00 ± 0.090.21 ± 0.17 to 0.20 ± 0.02 /ml, 88.85 ± 0.58 to 72.70 ± 0.54% /ml, 11.14 ± 0.58 to 27.29 ± 0.54%, 81.00 ± 0.78 to 66.22 ± 0.61%,9.03 ± 0.42 to 13.96 ± 0.47%, 9.70 ±  to 13.00 ± 0.30 and 21.70 ± 0.59 to 30.40 ± 0.53% for the indigenous and Amo breed groups of cockerel, respectively. It was concluded that semen quality characteristics could be differed between genetically improved (Amo strain) and indigenous breed of cockerels.

Fertility following synchronization of oestrus in the sheep with intravaginal sponges. II. Effect of dose of progestagen and rate of absorption

1970 ◽  
Vol 21 (5) ◽  
pp. 783 ◽  
Author(s):  
TJ Robinson
Keyword(s):  
Linear Relationship ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Great Variability ◽  
Significant Linear Relationship ◽  
Rate Of Absorption ◽  
Time Basis ◽  
Dose Levels

The effect on fertility of the amount of Cronolone (Searle) absorbed from intravaginal sponges and the method of artificial insemination was studied. A significant linear relationship exists between fertility and the amount absorbed in 16 days, over the range 1-10 mg. No such relationship can be demonstrated above this amount, and great variability and sensitivity to failure of fertility is shown at all dose levels. Insemination on a fixed time basis is superior to that on teasing. Of ewes inseminated on a time basis on the second or third day after withdrawal of sponges, 117 lambed of 237 treated (49.4%). Of ewes inseminated on detection of oestrus, 97 lambed of 196 in oestrus (49.5 %) of 249 treated (39 .O %).

scholarly journals Some Aspects of Reproductive Performance in Inbred Mice

1968 ◽  
Vol 2 (1) ◽  
pp. 89-100 ◽  
Author(s):  
Michael Festing
Keyword(s):  
Linear Regression ◽  
Linear Relationship ◽  
Litter Size ◽  
Inbred Mice ◽  
Strain Differences ◽  
The Third ◽  
Significant Linear Relationship ◽  
Size Interval ◽  
Genetically Determined ◽  
Linear Decline

An examination of litter size, interval between litters, the ‘litter index’ (a measure of the persistency of breeders), preweaning mortality and productivity 70, 120 and 170 days after mating was made in 9 inbred lines of mice. The data were collected over a period of at least a year from the breeding records of 360 monogamous pairs of mice. Litter size was not significantly affected by parity. There were significant strain differences in the pattern of intervals between litters. Typically, the interval between mating and the first litter was short, followed by a longer interval between the first and second litter, followed again by a shorter interval both between the second and third and between the third and fourth litters. This pattern was most pronounced in the BALB/c, but did not occur in the DBA/2, where the intervals between all litters were approximately equal. There were statistically significant strain differences in all seven characters, and heritabilities in the broad sense ranged from 0.36 for the interval between litters to 0.89 for preweaning mortality. The heritabilities of the other three components of 170–day productivity (litter size, litter index and interval between litters) were less than the heritability of 170-day productivity itself. The shape of the productivity curve was found to be genetically determined, although in the typical case there was a linear decline in productivity from the first to the third 50–day period. No significant linear relationship was found between litter size or interval between litters and 170–day productivity using simple linear regression. There was a significant linear relationship between 170–day productivity and preweaning mortality, and between 170-day productivity and the litter index. Multiple linear regression showed that the four factors: litter size, interval between litters, preweaning mortality and litter index, accounted for about 32, 10, 32 and 23 per cent respectively of the variation in 170-day productivity.

scholarly journals Assessment of Semen Characteristics Among Three Phenotypes of Chicken Raised in Akko, Gombe State of Nigeria

2021 ◽  
Vol 12 (1) ◽  
pp. 40-48
Author(s):  
Tijjani Haruna Usman ◽  
Saleh Mohammed Sir ◽  
Sadiq Haladu ◽  
Asmad Kari
Keyword(s):  
Artificial Insemination ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Sperm Abnormalities ◽  
Normal Sperm ◽  
Poultry Breeding ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Abdominal Massage

In poultry breeding program the success of artificial insemination (AI) is highly influenced by  the quality of semen. The aim of the present research was to evaluate the variations in semen characteristics of three phenotypes of chicken of the same specie raised in Akko, Nigeria. A total of 9 cocks from threedifferent phenotypes; Red feathered (n = 3), White feathered (n = 3) and Black feathered (n =3). The semen was evaluated for macroscopic (i.e. semen volume and colour) and microscopic (i.e. sperm concentration, motility and morphology) criteria after being collected by abdominal massage method. There were phenotypes variation (P > 0.05)effects on semen motility, number of live/dead sperm and sperm abnormalities. No differences(P>0.05) were observed on volume, colour, concentration, mass motility and pH of semen. The observed sperm progressive motility was ranged from 90.5 ±1.21% to 95.09±0.82%. The White feathered strain had the highest sperm progressive motility (P<0.05)and highest value for live and normal sperm (93.5±0.63% and 87.90±0.25%), while the black feathered had the least and (92.4±0.73% and 85.5±0.50%) respectively. The Red feathered strain had the highest value for both dead and sperm head defect (9.2±0.33%and 7.0±0.52%). Whilst, the Black feathered strain scored the highest percentage of both sperm tail and neck defects with values ranged from 11.4±0.43%to 14.2±1.08% and 19.4±0.53% to 23.6±0.69%, respectively. This study suggests that there are large variations present in semen characteristics of different phenotypes of cocks; White feathered strain is likely have better semen characteristics compared to Red and Black feathered strains. Therefore, White feathered strain can potentially be used in artificial insemination (AI) for chicken production and improvement.

Studies in controlled artificial insemination of Merino sheep

1958 ◽  
Vol 9 (5) ◽  
pp. 693 ◽  
Author(s):  
TJ Robinson
Keyword(s):  
Artificial Insemination ◽  
Treatment Effects ◽  
Factorial Experiment ◽  
Merino Sheep ◽  
High Incidence ◽  
Lambing Rate

A total number of 507 ewes were inseminated at the rate of approximately 100 per day for 5 days, in a factorial experiment with variables: rams (5), treatments (5), days of insemination (5), volume of semen (2). Treatments were: (1) control; (2) progesterone daily for 16 days, PMS on 17th, inseminated on teasing; (3) progesterone every 2 days, PMS on 17th, inseminated on teasing; (4) as for (2), but inseminated 2 days after PMS; (5) as for (3), but inseminated 2 days after PMS. The overall lambing rate was low (31-6 per cent.). There were significant differences between rams, treatments, and day of insemination. Relative infertility of one ram was related to high incidence of sperm with curled tails. Treatment effects were: (1) 40.8 per cent. of ewes lambed; (2) 33.7 per cent.; (3) 32.9 per cent.; (4) 30.2 per cent.; (5) 15.0 per cent. Insemination was less efficient on the first day. Volume of undiluted semen used (0.05 or 0.1 ml) had no effect.

The artificial insemination of the Merino sheep following the synchronization of oestrus and ovulation by progesterone injected alone and with Pregnant Mare Serum Gonadotrophin (PMS)

1956 ◽  
Vol 7 (3) ◽  
pp. 194 ◽  
Author(s):  
TJ Robinson
Keyword(s):  
Breeding Season ◽  
Artificial Insemination ◽  
Merino Sheep ◽  
The Third ◽  
Final Injection ◽  
Group 3 ◽  
Group 2 ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Group 1 ◽  
Lambing Rate

Two groups (2 and 3), each of 112 stud Merino ewes, which were experiencing regular oestrous cycles, received daily injections of 10 mg progesterone for 16 days, in an attempt to synchronize oestrus and ovulation for artificial insemination. One group (3) received an additional injection of 500 i.u. pregnant mare serum gonadotrophin (PMS) the day after the final progesterone injection. Injections were staggered so as to bring between 15 and 20 ewes of each group into oestrus on each of six days, on which days approximately equal numbers of untreated oestrous ewes were inseminated (group 1). In group 2 (progesterone alone), 104 ewes were teased and inseminated within 4 days of the final injection, including 74 (71 per cent.) on the third day. Corresponding numbers of ewes for group 3 (progesterone-PMS) were 107, 96 (90 per cent.). Injection of PMS significantly advanced and improved the precision of time of onset of oestrus, and presumably of ovulation. Of 315 ewes inseminated in all groups, 265 received 0.10 ml undiluted semen; 33, 0.05 ml; and 17, 0.20 ml. There were no apparent differences in lambing rate. Ewes inseminated, ewes lambing, and lambs born were: group 1, 104, 58 (55 per cent.), 74 (71 per cent.); group 2, 104, 53 (51 per cent.), 64 (62 per cent.); group 3, 107, 61 (57 per cent.), 85 (79 per cent.); overall, 315, 172 (55 per cent.), 223 (71 per cent.). There were no significant differences between groups in the proportion of ewes which lambed, or in the twinning rate. Ewes in oestrus and inseminated 72 hr after the final injection of progesterone had a significantly higher lambing rate than did ewes inseminated a day later. It is concluded that progesterone, particularly if used in conjunction with PMS, is of value in synchronizing oestrus in the breeding season for precisely planned artificial insemination.

scholarly journals Effect of breeds on semen characteristics of aged bulls in the Indonesian National Artificial Insemination Center

2020 ◽  
Vol 30 (2) ◽  
pp. 173-179
Author(s):  
Irida Novianti ◽  
Bambang Purwantara ◽  
Enniek Herwijanti ◽  
Chairdin Dwi Nugraha ◽  
Rafika Febriani Putri ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Semen Characteristics

Further investigations on artificial insemination of sheep

1941 ◽  
Vol 31 (3) ◽  
pp. 354-369 ◽  
Author(s):  
J. Anderson
Keyword(s):  
Artificial Insemination ◽  
High Grade ◽  
Merino Sheep

The results obtained from artificial insemination of high grade Merino sheep in Kenya are given and discussed in relation to the season of the year, time of insemination during oestrus, the number of inseminations in each oestrous period and the degree of dilution of sperm.

Studies on the number of ewes joined per ram for flock matings under paddock conditions. II. The effect of mating on semen characteristics

1968 ◽  
Vol 19 (6) ◽  
pp. 1043 ◽  
Author(s):  
RJ Lightfoot
Keyword(s):  
Similar Extent ◽  
Sperm Density ◽  
Semen Collection ◽  
Abnormal Sperm ◽  
Semen Characteristics

Semen characteristics were studied prior to and during the paddock joining of rams to varying numbers of ewes in a series of three experiments. A marked fall in ejaculate volume, sperm density, and their product, total number of sperm per ejaculate to levels of c. 0.3 ml, 1.5 x l09 sperm/ml, and 0.45 x l09 total sperm respectively occurred during the first 4 days of joining. Although there were large differences in the number of ewes joined per ram (25 to 100) and consequently the number of ewes served per ram per day, the depression in these semen characteristics occurred to a similar extent in all rams during the first 2 weeks of mating. By examining the data for all collections throughout the joining period, however, highly significant negative correlations were obtained for ejaculate volume, sperm density, and total number of sperm per ejaculate in each case with the number of ewes served during the 4 days preceding semen collection. Increases in the proportion of abnormal sperm were found sporadically in the ejaculates of rams during joining. Rams 2½ years old produced significantly more sperm per ejaculate before, during, and after the 6 week joining period than rams 1½ years old.

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