Studies on resistance of sheep to infestation with Haemonchus contortus and Trichostrongylus spp. and on the immunological reactions of sheep exposed to infestation. VI. The influence of age and nutrition on resistance to Trichostrongylus colubriformis.

1953 ◽  
Vol 4 (3) ◽  
pp. 340 ◽  
Author(s):  
DF Stewart ◽  
HMcL Gordon
Keyword(s):  
Haemonchus Contortus ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
Previous Exposure ◽  
Trichostrongylus Colubriformis ◽  
The Poor ◽  
Experimental Infestation ◽  
Immunological Reactions ◽  
Striking Contrast

Of 10 sheep aged 3-4 years which had been born and maintained under worm-free conditions, 7 died from an initial experimental infestation with Trichostrongylus colubriformis. This mortality was in striking contrast to the results with nine-months-old sheep which had had previous experience of infestation. It demonstrated that resistance to T. colubriformis could not be ascribed to increasing age. In a group fed on a low plane of nutrition and given 40,000 or 100,000 T. colubriformis larvae, more sheep developed infestations than did similar sheep in groups given an adequate or a high-protein diet. in lambs which had been kept worm-free since birth and then dosed with 20,000 T. colubriformis larvae, infestation developed almost equally in those fed on the poor diet and those fed on the high-protein diet. The groups fed on the low plane of nutrition successfully withstood subsequent challenge doses of up to 300,000 T. colubriformis larvae. In these experiments, previous exposure to infestation apparently exercised more influence on resistance than did diet.

Effect of dietary tannin and protein concentration on nematode infection (Trichostrongylus colubriformis) in lambs

2000 ◽  
Vol 134 (1) ◽  
pp. 89-99 ◽  
Author(s):  
N. L. BUTTER ◽  
J. M. DAWSON ◽  
D. WAKELIN ◽  
P. J. BUTTERY
Keyword(s):  
High Protein Diet ◽  
Diet Group ◽  
High Protein ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Trichostrongylus Colubriformis ◽  
Low Protein ◽  
Faecal Egg Counts ◽  
Group 2 ◽  
Protein Diets

The aim of this study was to determine whether the inclusion of a condensed tannin (quebracho tannin, QT) and/or the elevation of dietary protein could reduce Trichostrongylus colubriformis establishment and existence in the small intestine of lambs. Thirty-six lambs (mean liveweight 32·6±3·9 kg) were randomly allocated to one of six experimental groups, groups 1–5 were parasitized with a trickle infection of 3000 infective Trichostrongylus colubriformis larvae daily, whilst group 6 remained as uninfected controls. Experimental diets were formulated to contain 222 g CP/kg (high protein) or 97 g CP/kg (low protein) with or without the inclusion of 50 g QT/kg. All six animal groups were fed the low protein diet, group 2 fed low protein diet+QT, for one month prior to infection (groups 1–5). Once nematode eggs were observed in the faeces, diets were abruptly changed in three experimental groups. Group 1 remained on the low protein diet, group 2 remained on the low protein+QT diet, group 3 changed to the high protein diet, group 4 changed to the high protein+QT diet, group 5 changed to the low protein+QT diet and group 6 remained uninfected and fed the low protein diet. Production, haematological and parasitological parameters were monitored at regular intervals. Results show that parasitized animals fed the high protein diet achieved growth rates similar to those of uninfected low protein-fed lambs. Inclusion of dietary QT did not depress liveweight gain. Total daily faecal egg counts declined after feeding the high protein diet. Inclusion of QT into the low protein diet also reduced faecal egg counts to similar levels observed in the high protein-fed lambs. The inclusion of QT into the high protein diet did not further reduce faecal egg counts. No significant differences in the haematological parameters measured were observed between infected animals (groups 1–5), suggesting that the beneficial effect of dietary QT in the low protein diet is unlikely to be mediated through an immune response. These data suggest that the inclusion of QT in low protein diets may be an alternative to feeding high protein diets to reduce nematode burden in lambs.

scholarly journals A high-protein diet increases faecal IgA concentrations in baby pigs

2008 ◽  
Vol 67 (OCE5) ◽  
Author(s):  
F. Vitari ◽  
A. Morise ◽  
M. Formal ◽  
C. Garcia ◽  
K. Mace ◽  
...  
Keyword(s):  
High Protein Diet ◽  
High Protein ◽  
Protein Diet

scholarly journals Measurement of the Intestinal pH in Mice under Various Conditions Reveals Alkalization Induced by Antibiotics

Antibiotics ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 180
Author(s):  
Kouki Shimizu ◽  
Issei Seiki ◽  
Yoshiyuki Goto ◽  
Takeshi Murata
Keyword(s):  
Antibiotic Treatment ◽  
High Protein Diet ◽  
Intestinal Bacteria ◽  
High Protein ◽  
Protein Diet ◽  
Germ Free ◽  
Treatment Regimens ◽  
Ph Values ◽  
Specific Pathogen ◽  
The Stability

The intestinal pH can greatly influence the stability and absorption of oral drugs. Therefore, knowledge of intestinal pH is necessary to understand the conditions for drug delivery. This has previously been measured in humans and rats. However, information on intestinal pH in mice is insufficient despite these animals being used often in preclinical testing. In this study, 72 female ICR mice housed in SPF (specific pathogen-free) conditions were separated into nine groups to determine the intestinal pH under conditions that might cause pH fluctuations, including high-protein diet, ageing, proton pump inhibitor (PPI) treatment, several antibiotic treatment regimens and germ-free mice. pH was measured in samples collected from the ileum, cecum and colon, and compared to control animals. An electrode, 3 mm in diameter, enabled accurate pH measurements with a small amount of gastrointestinal content. Consequently, the pH values in the cecum and colon were increased by high-protein diet, and the pH in the ileum was decreased by PPI. Drastic alkalization was induced by antibiotics, especially in the cecum and colon. The alkalized pH values in germ-free mice suggested that the reduction in the intestinal bacteria caused by antibiotics led to alkalization. Alkalization of the intestinal pH caused by antibiotic treatment was verified in mice. We need further investigations in clinical settings to check whether the same phenomena occur in patients.

scholarly journals The Colonic Microbiome and Epithelial Transcriptome Are Altered in Rats Fed a High-Protein Diet Compared with a Normal-Protein Diet

2016 ◽  
Vol 146 (3) ◽  
pp. 474-483 ◽  
Author(s):  
Chunlong Mu ◽  
Yuxiang Yang ◽  
Zhen Luo ◽  
Leluo Guan ◽  
Weiyun Zhu
Keyword(s):  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
Normal Protein Diet

scholarly journals Effect of a High Protein Diet on Calcium Metabolism in Sheep

1991 ◽  
Vol 62 (7) ◽  
pp. 628-635
Author(s):  
Masayuki FUNABA ◽  
Hajime NABETA ◽  
Hideo YANO ◽  
Ryoji KAWASHIMA
Keyword(s):  
Calcium Metabolism ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet

Interactions of insulin-like growth factor (IGF)-II and growth hormone in vivo: circulating levels of IGF-I and IGF-binding proteins in transgenic mice

1997 ◽  
pp. 701-708 ◽  
Author(s):  
A Blackburn ◽  
RA Dressendorfer ◽  
WF Blum ◽  
M Erhard ◽  
G Brem ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Transgene Expression ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
Standard Diet ◽  
Igf I ◽  
Igf Binding ◽  
B Mice

To study interactions between insulin-like growth factor-II (IGF-II) and growth hormone (GH) in vivo, we crossed hemizygous transgenic mice carrying phosphoenolpyruvate carboxykinase (PEPCK)-IGF-II fusion genes with hemizygous PEPCK-bovine GH (bGH) transgenic mice. Offspring harbouring both transgenes (IB), the IGF-II transgene (I) or the bGH transgene (B), and non-transgenic littermates (C) were obtained. Blood samples were taken before (end of week 12) and after (end of week 14) the mice had received a diet high in protein and low in carbohydrates to stimulate PEPCK promoter-controlled transgene expression. Mean serum GH concentrations of both B and IB mice corresponded to 900 ng/ml and increased more than twofold (P < 0.001) after 1 week of the high-protein diet. GH concentrations in controls and I mice were less than 20 ng/ml. Serum IGF-II concentrations in I and IB mice were three-to fourfold higher than those in C and B mice. Whereas IGF-II concentrations were not changed by the high-protein diet in the last two groups, serum IGF-II increased significantly in I (P < 0.001) and IB mice (P < 0.05). This increase was significantly (P < 0.05) less pronounced in IB than in C and I mice. Circulating IGF-I concentrations were about twofold (P < 0.001) higher in B and IB than in C and I mice, and showed a tendency to be lower in I than in C and in IB than in B mice when animals were maintained on the standard diet. The high-protein diet did not change circulating IGF-I concentrations in controls and B mice, but resulted in a significant reduction of serum IGF-I concentrations in I (P < 0.05) and IB mice (P < 0.001). Consequently, after PEPCK-IGF-II transgene expression was stimulated, serum IGF-I concentrations were significantly (P < 0.05) lower in I than in C and in IB than in B mice. Serum IGF-binding protein (IGFBP)-2 concentrations were significantly (P < 0.05) higher in I mice than in all other groups when mice were maintained on the standard diet, with a tendency to reduced IGFBP-2 concentrations in B mice. After the high-protein diet, serum IGFBP-2 concentrations did not change in C and I mice, but increased by two- to threefold in B and IB mice (P < 0.001). Serum IGFBP-3 concentrations tended to be greater in B and IB than in C and I mice, but these differences were mostly not significant. IGFBP-4 concentrations were significantly (P < 0.001) increased by GH overproduction in B and IB mice. Our data suggest that the reduction in circulating IGF-I concentrations by increased IGF-II is most probably due to the limited serum IGF binding capacity and the short half-life of free IGFs, rather than to a reduction in GH-dependent IGF-I production. Effects of GH overproduction on serum IGFBP-2 concentrations depend on dietary factors and may be both inhibitory and stimulatory.

scholarly journals Adaptive changes in the capacity of systems used for the synthesis of citrulline in rat liver mitochondria in response to high- and low-protein diets

1974 ◽  
Vol 142 (2) ◽  
pp. 359-364 ◽  
Author(s):  
J. D. McGivan ◽  
Norah M. Bradford ◽  
J. B. Chappell
Keyword(s):  
Nitrogen Source ◽  
High Protein Diet ◽  
Liver Mitochondria ◽  
High Protein ◽  
Protein Diet ◽  
Rat Liver Mitochondria ◽  
Standard Diet ◽  
Adaptive Changes ◽  
Low Protein ◽  
Citrulline Synthesis

1. Citrulline synthesis was measured in mitochondria from rats fed on a standard diet, a high-protein diet, or on glucose. 2. With NH4Cl as the nitrogen source the rate of citrulline synthesis was higher in mitochondria from rats fed on a high-protein diet than in those from rats fed on a standard diet. When rats were fed solely on glucose the rate of synthesis of citrulline from NH4Cl was very low. 3. With glutamate as the nitrogen source the relative rates of citrulline synthesis were much lower than when NH4Cl was present, but similar adaptive changes occurred. 4. The activity of the mitochondrial glutamate-transporting system increased two to three times on feeding rats on a high-protein diet, but the Km for glutamate was unchanged. 5. Adaptive changes in certain intramitochondrial enzymes were also measured. 6. The results were interpreted to indicate that when an excess of substrate was present, citrulline synthesis from NH4Cl was rate-limited by the intramitochondrial concentration of N-acetyl-glutamate, but citrulline synthesis from glutamate was rate-limited primarily by the activity of the glutamate-transporting system.

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