Minimising cold damage during reproductive development among temperate rice genotypes. II. Genotypic variation and flowering traits related to cold tolerance screening

2006 ◽  
Vol 57 (1) ◽  
pp. 89 ◽  
Author(s):  
T. C. Farrell ◽  
K. M. Fox ◽  
R. L. Williams ◽  
S. Fukai ◽  
L. G. Lewin
Keyword(s):  
Low Temperature ◽  
Cold Tolerance ◽  
Field Experiments ◽  
Cold Water ◽  
Genotypic Variation ◽  
Pollen Grains ◽  
Microspore Development ◽  
Spikelet Sterility ◽  
Temperature Conditions ◽  
Anther Length

Low temperature during microspore development increases spikelet sterility and reduces grain yield in rice (Oryza sativa L.). The objectives of this study were to determine genotypic variation in spikelet sterility in the field in response to low temperature and then to examine the use of physio-morphological traits at flowering to screen for cold tolerance. Multiple-sown field experiments were conducted over 4 consecutive years in the rice-growing region of Australia to increase the likelihood of encountering low temperature during microspore development. More than 50 cultivars of various origins were evaluated, with 7 cultivars common to all 4 years. The average minimum temperature for 9 days during microspore development was used as a covariate in the analysis to compare cultivars at a similar temperature. The low-temperature conditions in Year 4 identified cold-tolerant cultivars such as Hayayuki and HSC55 and susceptible cultivars such as Sasanishiki and Doongara. After low temperature conditions, spikelet sterility was negatively correlated with the number of engorged pollen grains, anther length, anther area, anther width, and stigma area. The number of engorged pollen grains and anther length were found to be facultative traits as their relationships with spikelet sterility were identified only after cold water exposure and did not exist under non-stressed conditions.

The interaction of nitrogen application and temperature during reproductive stage on spikelet sterility in field-grown rice

2005 ◽  
Vol 56 (6) ◽  
pp. 625 ◽  
Author(s):  
T. A. Gunawardena ◽  
S. Fukai
Keyword(s):  
Low Temperature ◽  
Grain Yield ◽  
Field Experiments ◽  
Pollen Grains ◽  
Strongly Correlated ◽  
Microspore Development ◽  
Spikelet Sterility ◽  
South Wales ◽  
Sowing Dates ◽  
N Rates

Increased grain yield in response to high rates of application of nitrogen (N) fertiliser is often limited by increased spikelet sterility, particularly under low temperature conditions in the New South Wales (NSW) rice industry. In 3 field experiments, different N rates were applied for different sowing dates to investigate the interaction between N rate and temperature during microspore development on spikelet sterility and grain yield. In one experiment the effect of water depth on spikelet sterility was also investigated. Engorged pollen production, spikelet sterility, and yield and its components were recorded. Application of N affected a few different processes that lead into spikelet sterility. Application of N at both pre-flood (PF) and panicle initiation (PI) significantly reduced the number of engorged pollen grains per anther, which was negatively correlated with spikelet sterility. Application of N and low temperature during microspore development with the absence of deep water also decreased pollen engorgement efficiency (the percentage of pollen grains that were engorged). Application of N further increased spikelet density, which, in turn, increased both spikelet sterility and grain yield. The combined effect of spikelet density and low temperature during microspore development explained the 44% of variation in the number of engorged pollen grains per anther. Grain yield was decreased by low temperature during microspore development in the shallow water when N was applied. Spikelet sterility as a result of late sowing was strongly correlated with minimum temperature during flowering. It is concluded that N application reduced pollen number per anther as a result of increased spikelet density, and this made the spikelets more susceptible to low temperature, causing increased spikelet sterility.

Low temperature induced spikelet sterility in rice. II. Effects of panicle and root temperatures

2003 ◽  
Vol 54 (10) ◽  
pp. 947 ◽  
Author(s):  
T. A. Gunawardena ◽  
S. Fukai ◽  
F. P. C. Blamey
Keyword(s):  
Low Temperature ◽  
Low Temperatures ◽  
Oryza Sativa L ◽  
Pollen Grains ◽  
Development Stage ◽  
Dominant Factor ◽  
Microspore Development ◽  
Root Temperature ◽  
Spikelet Sterility ◽  
Germinated Pollen

Low temperatures impose restrictions on rice (Oryza sativa L.) production at high latitudes. This study is related to low temperature damage that can arise mid-season during the panicle development phase. The objective of this study was to determine whether low temperature experienced by the root, panicle, or foliage is responsible for increased spikelet sterility. In temperature-controlled glasshouse experiments, water depth, and water and air temperatures, were changed independently to investigate the effects of low temperature in the root, panicle, and foliage during microspore development on spikelet sterility. The total number of pollen and number of engorged pollen grains per anther, and the number of intercepted and germinated pollen grains per stigma, were measured. Spikelet sterility was then analysed in relation to the total number of pollen grains per spikelet and the efficiency with which these pollen grains became engorged, were intercepted by the stigma, germinated, and were involved in fertilisation. There was a significant combined effect of average minimum panicle and root temperatures on spikelet sterility that accounted for 86% of the variation in spikelet sterility. Total number of pollen grains per anther was reduced by low panicle temperature, but not by low root temperature. Whereas engorgement efficiency (the percentage of pollen grains that were engorged) was determined by both root and panicle temperature, germination efficiency (the percentage of germinated pollen grains relative to the number of engorged pollen grains intercepted by the stigma) was determined only by root temperature. Interception efficiency (i.e. percentage of engorged pollen grains intercepted by the stigma), however, was not affected by either root or panicle temperature. Engorgement efficiency was the dominant factor explaining the variation in spikelet sterility. It is concluded that both panicle and root temperature affect spikelet sterility in rice when the plant encounters low temperatures during the microspore development stage.

scholarly journals A method for evaluating cold tolerance in rice during reproductive growth stages under natural low-temperature conditions in tropical highlands in Kenya

2020 ◽  
Vol 23 (4) ◽  
pp. 466-476
Author(s):  
Hiroaki Samejima ◽  
Mayumi Kikuta ◽  
Keisuke Katura ◽  
Daniel Menge ◽  
Emily Gichuhi ◽  
...  
Keyword(s):  
Low Temperature ◽  
Cold Tolerance ◽  
Growth Stages ◽  
Reproductive Growth ◽  
Temperature Conditions ◽  
Tropical Highlands

Rice-cold tolerance across reproductive stages

2016 ◽  
Vol 67 (8) ◽  
pp. 823 ◽  
Author(s):  
J. H. Mitchell ◽  
S. L. Zulkafli ◽  
J. Bosse ◽  
B. Campbell ◽  
P. Snell ◽  
...  
Keyword(s):  
Cold Stress ◽  
Cold Tolerance ◽  
Oryza Sativa L ◽  
Genotypic Variation ◽  
Flowering Stage ◽  
Spikelet Sterility ◽  
Booting Stage ◽  
Cold Tolerant ◽  
Tolerance Response ◽  
F2 Generation

Cold temperature stress at the reproductive stage, particularly at booting and flowering stages can cause significant reductions in rice (Oryza sativa L.) yield particularly at high latitudes and elevation. Although genotypic variation for cold tolerance is known to exist, the tolerance mechanisms and genotypic consistency across the stages are yet to be understood for segregating populations. Three experiments were conducted under controlled temperature glasshouse conditions to determine floral characteristics that were associated with cold tolerance at the flowering stage and to determine genotypic consistency at the booting and flowering stages. Twenty F5 Reiziq × Lijiangheigu lines from two extreme phenotypic bulks selected for cold tolerance at booting stage in the F2 generation were utilised. Spikelet sterility under cold stress at booting was significantly correlated with spikelet sterility under cold stress at flowering (r = 0.62**) with five lines identified as cold tolerant across reproductive stages. There was also a positive correlation (r = 0.47*) between spikelet sterility under cold stress at booting at the F5 and at the F2 generation. The quantitative trait loci (QTL; qLTSPKST10.1) previously identified on chromosome 10 contributing to spikelet sterility within the F2 generation, was also identified in the F5 generation. Additionally, genomic regions displaying significant segregation between the progenies contrasting for their cold tolerance response phenotype were identified on chromosomes 5 and 7 with Lijiangheigu as allelic donor and an estimated reduction in spikelet sterility of 25% and 27%, respectively. Although genotypic variation in spikelet sterility at the booting stage was not related to the development rate for heading or flowering, those cold-tolerant genotypes at the flowering stage were the quickest to complete flowering. Cold-tolerant genotypes at the flowering stage had larger numbers of dehisced anthers and subsequently pollen number on stigma, which contributed to reduced spikelet sterility. It is concluded that enhanced anther dehiscence plays a significant role in improved cold tolerance at the flowering stage.

scholarly journals Phenotypic Variation and Selection for Cold-Tolerant Rice (Oryza sativa L.) at Germination and Seedling Stages

Agriculture ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 162 ◽  
Author(s):  
Doan Cong Dien ◽  
Takeo Yamakawa
Keyword(s):  
Low Temperature ◽  
Cold Tolerance ◽  
Seedling Stage ◽  
Germination Index ◽  
Rice Varieties ◽  
Coleoptile Length ◽  
Temperature Conditions ◽  
Cold Tolerant ◽  
Germination Stage ◽  
Radicle Length

Owing to its origin in tropical and subtropical areas, rice is susceptible to cold stress. Low temperatures at the germination and seedling stages can result in seed loss, a delayed transplanting period, and lower final yield. In this study, 181 rice varieties from around the world were investigated for cold tolerance at the germination and seedling stages. At the germination stage, the responses of different rice varieties were examined based on the germination index, coleoptile length, and radicle length at low (13 °C) and control temperatures (25 °C). Significant variations in the germination index, coleoptile length, and radicle length were observed among varieties. Low temperature significantly decreased germination ability, and coleoptile and radicle growth in the studied varieties. At the seedling stage, cold tolerance of the rice varieties was evaluated based on the leaf color score under natural low temperature. Similar to the germination stage, at the seedling stage, significant variation in root and shoot growth was observed in the response of rice varieties to low temperature conditions. Based on the results from both the germination and seedling stages, two varieties (Hei-Chiao-Chui-Li-Hsiang and Ta-Mao-Tao) were selected as the best cold-tolerant varieties. Our results also indicate the benefits of warming treatments to protect rice seedlings from low temperature conditions.

Low temperature induced spikelet sterility in rice. I. Nitrogen fertilisation and sensitive reproductive period

2003 ◽  
Vol 54 (10) ◽  
pp. 937 ◽  
Author(s):  
T. A. Gunawardena ◽  
S. Fukai ◽  
F. P. C. Blamey
Keyword(s):  
Low Temperature ◽  
Early Stage ◽  
Pollen Grains ◽  
Reproductive Period ◽  
Pollen Production ◽  
Spikelet Number ◽  
Spikelet Sterility ◽  
Cell Stage ◽  
Panicle Development ◽  
Germinated Pollen

Low temperature during panicle development in rice increases spikelet sterility. This effect is exacerbated by high rates of nitrogen (N) application in the field. Spikelet sterility induced by low temperature and N fertilisation was examined in glasshouse experiments to clarify the mechanisms involved. In two glasshouse experiments, 12-h periods of low (18/13�C) and high (28/23�C) day/night temperatures were imposed over periods of 5–7 days during panicle development, to determine the effects of low temperature and N fertilisation on spikelet sterility. In one experiment, 50% sunlight was imposed together with low temperature to investigate the additive effects of reduced solar radiation and low temperature. The effect of increased tillering due to N fertilisation was examined by a tiller removal treatment in the same experiment. Pollen grain number and spikelet sterility were recorded at heading and harvest, respectively. Although there was no significant effect of low temperature on spikelet sterility in the absence of applied N, low temperature greatly increased spikelet sterility as a result of a reduction in the number of engorged pollen grains per anther in the presence of applied N. Spikelet sterility was strongly correlated with the number of engorged pollen grains per anther. Low temperature during very early (late stage of spikelet differentiation–pollen mother cell stage) and peak (second meiotic division stage–early stage of extine formation) microspore development caused a severe reduction in engorged pollen production mainly as a result of reduced total pollen production. Unlike low temperature, the effect of shading was rather small. The increased tillering due to application of high rates of N, increased both spikelet number per plant and spikelet sterility under low temperature conditions. The removal of tillers as they appeared reduced the number of total spikelets per plant and maintained a large number of engorged pollen grains per anther which, in turn, reduced spikelet sterility. The number of engorged pollen grains per anther determined the numbers of intercepted and germinated pollen grains on the stigma. It is concluded that N increased tillering and spikelet number per plant and this, in turn, reduced the number of engorged pollen grains per anther, leading into increased spikelet sterility under low temperature condition.

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