Feeding an energy supplement with white clover silage improves rumen fermentation, metabolisable protein utilisation, and milk production in dairy cows

2006 ◽  
Vol 57 (4) ◽  
pp. 367 ◽  
Author(s):  
D. C. Cohen ◽  
C. R. Stockdale ◽  
P. T. Doyle

Six rumen-fistulated Holstein-Friesian cows were used in a Latin square design to test the hypothesis that more frequent feeding of a high energy supplement to cows consuming high-protein white clover silage would improve microbial protein production, resulting in greater N retention and higher milk yields. The white clover silage (10.7 MJ metabolisable energy (ME)/kg DM) was fed to cows either alone (WCS) or with 4.5 kg DM of rolled barley grain (12.1 MJ ME/kg DM). The grain was offered either 24 times (WCS/24B) or twice daily (WCS/2B, at 0800 and 1700 hours). Cows offered the supplements, regardless of feeding frequency, had higher (P < 0.05) organic matter (17.3 v. 16.0 kg/day) and estimated ME (208 v. 189 MJ/day) intakes than cows offered white clover silage alone. Mean daily ruminal fluid pH (P < 0.05) and ammonia-N concentrations (P < 0.05) were lower in the supplemented treatments, with total VFA concentrations being highest (P < 0.05) in the WCS/2B treatment. Nitrogen intake and output in the faeces were similar for all 3 treatments. However, nitrogen excretion was lower (P < 0.05) in urine (174 v. 218 g/day) and higher (P < 0.05) in milk (115 v. 93 g/day) of cows offered the supplements. The crude protein consumed by cows on all 3 diets was estimated to be well in excess of cow requirements. The supplements reduced the calculated net losses of ammonia-N from the rumen from 25% of total crude protein intake for WCS to 14% in the 2 supplement treatments, and increased the metabolisable protein supply available for milk production. Increases in metabolisable protein were estimated to be due to a higher microbial crude protein contribution in the supplemented treatments compared with the WCS treatment. Grain supplements increased (P < 0.05) milk yield (22.4 v.19.6 kg/day) and although there were no significant differences in milk fat and protein concentrations between treatments, the latter tended to increase with grain supplementation. Milk yield was higher in the WCS/24B treatment than in the WCS/2B treatment, but neither the calculated nor the measured rumen variables were sufficiently different to explain this effect of frequency of feeding the grain. One possible explanation for the difference was the marked fluctuations in key rumen variables throughout the day in the WCS/2B compared with the WCS/24B treatment. Such fluctuations in the rumen environment are not accounted for in theoretical calculations since associative effects are not considered. The benefits of a higher milk production as a result of more frequent feeding of the supplement to cows should be considered in context of the additional effort or costs associated with more frequent feeding.

2001 ◽  
Vol 41 (4) ◽  
pp. 465 ◽  
Author(s):  
W. J. Wales ◽  
Y. J. Williams ◽  
P. T. Doyle

An experiment was undertaken to establish the marginal milk production response from cereal grain supplementation by dairy cows grazing irrigated perennial pastures in spring and whether the addition of a hay supplement would improve that response. Cows were offered perennial ryegrass (Lolium perenne L.)–white clover (Trifolium repensL.) pasture at allowances (measured to ground level) of 20 or 40 kg dry matter (DM)/cow.day. Additional cows offered the low allowance were also offered supplements of pasture hay as a cube or pellet (2.5 kg DM/day), cereal grain as a pellet (5.0 kg DM/day) or cereal grain plus pasture hay (7.5 kg DM/day) as a pellet (1.75:1 DM basis) or cube (1.97:1 DM basis). The 7 treatments were replicated 3 times, and 63 cows were used (3 cows/treatment). The in vitro DM digestibility, crude protein and neutral detergent fibre (NDF) concentration of the barley and perennial pasture hay were 863 and 563 g/kg DM, 131 and 92 g/kg DM, and 182 and 666 g/kg DM, respectively. Cows from all treatments consumed herbage with an in vitro DM digestibility of 833 g/kg DM, crude protein content of 214 g/kg DM and NDF concentration of 457 g/kg DM compared with the pregrazed herbage on offer of 771, 174 and 527 g/kg DM, respectively. The mean pregrazing herbage mass of 4.13 t DM/ha consisted of perennial ryegrass (507 g/kg DM), white clover (188 g/kg DM), weeds (194 g/kg DM) and dead material (111 g/kg DM). Without supplements, cows consumed 11.2 kg DM/day at the low pasture allowance and 15.6 kg DM/day at the high allowance. Herbage intake did not decline to a large degree when barley was included in the diet, where a substitution rate of 0.2 kg reduction in pasture DM/kg DM cereal grain supplement was measured. The NDF concentration of the diet consumed was lowest (P< 0.05) for the grain pellet treatment (370 g NDF/kg DM) and highest (P&lt;0.05) for the fibre pellet (493 g NDF/kg DM) and the fibre cube (507 g NDF/kg DM) treatments. Fat-corrected milk yield increased (P<0.05)) from 20.1 to 24.5 kg/cow.day when cows consumed 4.5 kg DM cereal grain/day with a marginal milk response of 1.0 kg 4% fat-corrected milk/kg DM concentrate supplement. Supplementation with grain or hay had no significant effect (P>0.05) on milk fat, protein or lactose concentrations. The cows offered the high allowance spent more (P<0.05) time grazing than those offered the low allowance, but there were no significant differences in rumination time. Cows spent more time ruminating when consuming the grain &plus; fibre cube compared with the other supplement treatments, which were lower (P<0.05) and similar. Cows offered pasture at the low allowance ruminated the least. The rumen pH was not different between treatments and averaged 5.9. The ratio of acetate plus butyrate: propionate was not different between treatments and averaged 4.3. This study has shown that feeding cereal grain to cows grazing pastures high in perennial ryegrass resulted in acceptable marginal milk responses of 1 kg milk/kg DM of cereal grain in spring because of sufficient NDF in the diet. As there was adequate NDF in the diet, further supplements with pasture hay as cubes or pellets had no effect on marginal milk responses .


Author(s):  
Ni Nyoman Suryani ◽  
I Wayan Suarna ◽  
Ni Putu Sarini ◽  
I Gede Mahardika

To determine the effect of energy levels on digestible nutrient, milk production and milk quality of 7 months pregnant Bali cattle, was the purpose of this study. The study was conducted in Bali, Province of Indonesia on 12 pregnant breeding phase of pre-calving (2 months before the birth) with the parent body weight 329-340 kg/head. The treatment given is four types of Metabolizable Energy (ME) levels: 2000, 2100, 2200 and 2300/kg respectively as treatment A, B, C, and D. All ration contain 10% of crude protein. Variables measured: energy intake, digestible nutrient, milk yield, and milk quality. This research is a randomized block design. The results showed that increase energy ration until 2300 kcal ME/kg would significantly (P<0.05) increase energy intake and highest at cattle consumed ratio D is 22239.55 kcal/day. However, digestible nutrient was not affected. Milk production increased with increasing energy rations and highest (P<0.05) at cattle received treatment D is 2179.83 ml/day compared to treatment A 936.67 ml/day. Milk fat and milk lactose also highest (P<0.05) in treatment D are 8.56% and 4.76% respectively. Based on these results, it can be concluded that increase energy ration will increase energy intake, milk yield and milk fat and milk lactose of Bali cattle. 


2017 ◽  
Vol 84 (4) ◽  
pp. 430-433 ◽  
Author(s):  
Jun Li ◽  
Aixin Liang ◽  
Zipeng Li ◽  
Chao Du ◽  
Guohua Hua ◽  
...  

This Research Communication describes the association between genetic variation within the prolactin (PRL) gene and the milk production traits of Italian Mediterranean river buffalo (Bufala mediterranea Italiana). High resolution melting (HRM) techniques were developed for genotyping 465 buffaloes. The association of genetic polymorphism with milk production traits was performed and subsequently the effects of parity and calving season were evaluated. Single nucleotide polymorphisms (SNPs) were identified at exons 2 and 5 and at introns 1 and 2. All the SNPs were in Hardy–Weinberg equilibrium, and statistical analysis showed that the polymorphism of intron1 was significantly (P < 0·05) associated with milk yield, milk protein content and peak milk yield. The average contribution of the intron1 genotype (r2intron1) to total phenotypic variance in milk production traits was 0·09, and the TT genotype showed lower values than CC and CT genotypes. A nonsynonymous SNP was identified in exon 2, which resulted in an amino acid change from arginine to cysteine. Moreover, the polymorphism of exon 2 was associated significantly with milk fat content (P < 0·05), and the buffaloes with TT genotype showed higher total fat content than the buffaloes with CT genotype. These findings provide evidence that polymorphisms of the buffalo PRL gene are associated with milk production traits and PRL can be used as a candidate gene for marker-assisted selection in Italian Mediterranean river buffalo breeding.


2018 ◽  
Vol 41 (1) ◽  
pp. 42552
Author(s):  
Fatima Kouri ◽  
Salima Charallah ◽  
Amina Kouri ◽  
Zaina Amirat ◽  
Farida Khammar

 This research aims to assess the performances of lactating Bedouin goat under arid conditions, focusing on the relationship of milk yield with body and udder morphology and growth traits. Fourteen goats and their kids were followed during early lactation (90 days post-partum). Mean daily milk yield was 0.56 kg. Milk fat, proteins, lactose, solids non-fat and minerals were respectively 34.9, 38.9, 48.8, 107 and 6.7 g L-1. Mean kids’ birth weight, adjusted body weight at 90 days of age and average daily gain were respectively 2 kg, 6.6 kg and 51.4 g day-1. Daily milk yield was correlated with goats’ body weight, withers height, rump height, udder width and udder circumference but not with linear udder score and growth performances. These results provide important data about the milk production of Bedouin goat emphasizing its correlation with body and udder morphological traits which can help to elaborate a specific breeding program for this breed. 


1995 ◽  
Vol 75 (4) ◽  
pp. 625-629 ◽  
Author(s):  
R. R. Corbett ◽  
L. A. Goonewardene ◽  
E. K. Okine

The effect of substituting peas for soybean and canola meals as a protein source in a high-producing dairy herd was studied in 66 Holstein cows, divided into two groups based on stage of lactation, parity, level of milk production and days in milk. Two 18.5% crude protein grain concentrate diets were formulated based on the nutrient analyses of the forages available. The control grain mix contained standard protein sources, principally soybean and canola meal (SBM\CM) while the test grain mix was formulated to contain approximately 25% field peas as the major source of protein. Both grain rations were formulated to the same nutrient specifications and balanced for undegradable protein. The duration of the trial was 6 mo during which grain feeding levels were adjusted monthly based on milk yield. For cows in early lactation, 4% fat-corrected milk yield was higher (P < 0.05) for cows fed pea based concentrates (31.3 kg d−1) than for cows fed SBM\CM supplement (29.7 kg d−1). Fat-corrected milk yield was not affected by source of protein in mid- and late-lactation cows. Fat-corrected milk production was not different (P > 0.05) for cows fed SBM\CM compared with cows fed the pea supplement when cows across all stages of lactation were included in the analyses. Milk fat percent was significantly higher (P < 0.05) for early- and mid-lactation cows fed the pea supplement. The results suggest that peas can be substituted for SBM\CM as a protein source for high-producing dairy cows. Key words: Dairy cow, pea, soybean and canola meal supplement, undegradable protein, milk production


Author(s):  
N. Kosyachenko ◽  
Marina Abramova ◽  
M. Lapina

Abstract. Aim. The study of the influence of crossbriding on the growth and development of heifers and subsequent productive and reproductive qualities of cows on the first lactation. Methods. Using statistical methods, correlation analysis and ANOVA for evaluated genetic indicators of economic-valuable traits of dairy cattle. In particular was studied live weight of heifers from birth to 18 months, first insemination age and first fecundation age, live weight at fecundation, frequency of insemination, first calving age, days open, live weight at first lactation, milk yield for 305 days (kg), fat (%, kg) and protein (%, kg) in milk. Results. Relationship between productive indicators and the genotype of the animal is established. When assessing the strength of the influence of the factor «blood on the Holstein breed» it was found that the greatest impact can be traced at the age of 6 and 18 months, and is respectively 12.7 % and 17.1 %.The reproductive qualities of the first-calf cows decreased with the increase percent of blood in the Holstein breed. Evaluation of the impact of crossbreeding on milk production at first lactation has revealed significant strong influence on milk yield for 305 days of first lactation, and related signs the amount of milk fat and protein. According to qualitative indicators, the low influence of the Holstein blood fraction was found, while to a greater extent on the fat content than on the protein content in milk. Evaluation of phenotypic and genotypic correlations of milk yield and quality components of milk revealed average negative phenotypic and low negative genotypic correlation in all groups. The exception was in heifers of Yaroslavl breed with percent of Holstein blood less than 50 %, in which the relationship of yield x protein was positive. The relationship between milk yield and fat and protein content in milk has the opposite moderate character and strength. Practical significance. Studies was established that breeding of animals of Holstein and Yaroslavl breeds in one herd allows to receive an optimum ratio of milk production with optimal percentage fat and protein. For this herd among animals of the improved genotypes of the Yaroslavl breed, the use of genotypes with a percent of blood in the Holstein breed from 75.1 % to 87.6 % is optimal, which should be taken into account when selecting bulls to the breeding program.


1977 ◽  
Vol 57 (4) ◽  
pp. 653-662 ◽  
Author(s):  
H. R. SHARMA ◽  
J. R. INGALLS ◽  
J. A. MCKIRDY

In experiment 1, 12 cows were used to compare the two (0–0) rapeseed meal (1788 and Tower) varieties with the commercial rapeseed meal (CRSM) and soybean meal (SBM). Feed intake, milk yield and fat content were not different (P > 0.05) among the four treatments; however, protein content was higher (P < 0.05) for the cows fed CRSM and SBM diets than for those fed the 1788–RSM diet. But more (P < 0.05) milk fat was produced by the cows fed 1788–RSM than by those fed CRSM and SBM diets. In experiment 2, eight cows were used to determine the effects of replacing SBM with Tower and also replacing a portion of Tower with urea (TU) in a mixed or extruded (TUE) form on feed intake, milk yield and nitrogen (N) retention. No differences were observed in feed consumption, milk yield or composition among the treatments. Serum thyroxine (T4) level was higher (P < 0.05) for the cows fed SBM than for those fed the 1788–SBM and was similar to levels for cows fed CRSM and Tower in the first experiment. However, no differences were found in thyroxine level in the second experiment. Extrusion of Tower–urea mixture increased (P < 0.05) the N retention compared with other treatments. These short-term studies suggest that up to 25% Tower RSM can be used in dairy rations without adverse effect on performance.


Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1379
Author(s):  
Robert Kupczyński ◽  
Antoni Szumny ◽  
Katarzyna Wujcikowska ◽  
Natalia Pachura

The aim of this paper is to review and systematize the current state of knowledge on glycol metabolism in cattle. Glycerol, derived from biodiesel production, must be purified in order to be a useful product for feeding livestock. The use of glycerol in the feeding of ruminants can be justified for several reasons: (i) it is a source of energy in the ration, (ii) it is a glucogenic precursor, and (iii) it may have an effect on milk composition. The high energy value of glycerol provides the opportunity to use this raw material as a partial grain substitute in cattle feed rations. Dietary supplementation of glycerol is associated with increased propionate, butyrate, valerate, and isovalerate concentrations in the rumen. Glycerol can be used at up to 10%–15% of the dietary dry matter (DM) and is well-established as a treatment for ketosis in cows. Glycerol increases plasma glucose and may reduce non-esterified fatty acids and β-hydroxybutyrate levels. The use of glycerol does not have a clear effect on DM intake, milk yield, or milk composition. However, some authors have reported an increase in milk yield after glycerol supplementation associated with decreased milk fat concentration. It is also possible that the concentration in the milk of odd-chain fatty acids and cis-9, trans-11 conjugated linoleic acid may increase after glycerol application.


Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1256
Author(s):  
Senén De La Torre-Santos ◽  
Luis J. Royo ◽  
Adela Martínez-Fernández ◽  
Cristina Chocarro ◽  
Fernando Vicente

The optimization of milk production includes a rational use of forages, respect for the environment and offers the best quality to consumers. Milk production based on grass and forages produces healthier milk and it is widely spread throughout the Atlantic arc to maximize milk yield per hectare. However, the mode of offering the grass can have a major influence on milk composition. The aim of this study was to evaluate the effect of grass supply mode (grazing, zero-grazing or ensiling) on dairy cows’ performance, with particular reference to fatty acids and fat-soluble antioxidants concentration. A three by three Latin square experiment was performed with 18 dairy cows. Experimental treatments consisted of exclusive feeding with grass silage and zero-grazing, both offered ad libitum indoors, or grazing for 24 h. The results showed that grazing cows had a higher dry matter intake and greater milk yield than cows feeding on grass silage and zero-grazing, as well as higher concentrations of protein, lactose, nonfat-solids and urea in milk than housed cows. Milk fat from grazing cows had a higher proportion of unsaturated fatty acids than from cows feeding on grass silage and zero-grazing, with significant differences in the proportion of vaccenic and rumenic acids. The 18:1 trans-11 to 18:1 trans-10 ratio is proposed as biomarker to identify the milk produced from the management system of grazing cattle. Milk from grazing cows had a greater proportion of lutein than cows eating grass silage, with the zero-grazing system having intermediate values. In conclusion, the mode of grass supply affects fatty acid and antioxidant profiles of milk.


2020 ◽  
Vol 60 (3) ◽  
pp. 347
Author(s):  
Adel H. M. Ibrahim ◽  
Nikolaos Tzanidakis ◽  
Smaragda Sotiraki ◽  
Huitong Zhou ◽  
Jonathan Hickford

Context Genetic selection based on genetic markers for economically important traits in Sfakia sheep. Aims The aim of the present study was to investigate variation in the ovine myostatin gene (MSTN) and calpain 3 gene (CAPN3), and their association with milk-production traits. Methods Records for milk yield, milk fat content, protein content, lactose content, and non-fat solid content, pH and somatic-cell score (log), were obtained from 376 Sfakia ewes. Polymerase chain reaction–single-strand conformational polymorphism (PCR–SSCP) analyses were used to detect variation in intron 1 of MSTN and exon 10 of CAPN3. General linear models were then used to test for associations between the variation in MSTN and CAPN3, and milk-production traits. Key results The SSCP banding patterns for MSTN showed four variants (A1, A2, A3 and A4), which contained nine nucleotide sequence differences. Four SSCP banding patterns (C1, C2, C3 and C4) were observed for CAPN3 and these contained eight nucleotide-sequence differences. The MSTN variation was associated (P &lt; 0.05) with variation in milk yield and non-fat milk solid content. Variation in CAPN3 was associated with milk yield (P &lt; 0.001), fat content (P &lt; 0.05) and lactose content (P &lt; 0.05). Association analyses between the presence/absence of MSTN and CAPN3 variants and milk-production traits showed that a variant of MSTN that had previously between associated with muscle hypertrophy was associated with decreased milk yield (P &lt; 0.05) and a lower non-fat milk solid content (P &lt; 0.01). A CAPN3 variant that had previously been associated with increased sheep-carcass loin lean-meat yield was associated with a decreased milk yield (P &lt; 0.01) and a decreased milk fat content (P &lt; 0.05). Conclusions Our results have provided an insight into the effects of variation in ovine MSTN and CAPN3 on milk-production traits in sheep. Implications To preserve the dual-purpose characteristics of Sfakia sheep, breeding goals should take into account the possible antagonism between meat and milk traits.


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