scholarly journals Schizochytrium limacinum SR-21 as a source of docosahexaenoic acid: optimal growth and use as a dietary supplement for laying hens

2006 ◽  
Vol 57 (1) ◽  
pp. 13 ◽  
Author(s):  
H. J. Chin ◽  
T. F. Shen ◽  
H. P. Su ◽  
S. T. Ding
Keyword(s):  
Docosahexaenoic Acid ◽  
Dietary Supplement ◽  
Egg Production ◽  
Laying Hens ◽  
Optimal Growth ◽  
Egg Yolk ◽  
Culture Conditions ◽  
Marine Fungus ◽  
Lipogenic Genes ◽  
Schizochytrium Limacinum

Culture conditions for the marine fungus Schizochytrium limacinum SR-21 (SR-21) to produce microbial docosahexaenoic acid (DHA) were evaluated, and the practicality of using this fungus product as a dietary supplement for laying Leghorn hens was investigated. The data showed that the cultured fungus produced high biomass and DHA. It generated 584 mg DHA/L of culture at the end of a 6-day culture. The fungus grew better at 25ºC than at 20ºC or 30ºC. With an increase in glucose concentration from 1% to 5% in the culture medium, biomass and DHA production were enhanced. A 6% glucose treatment reduced the biomass production compared with 5% glucose. A bioreactor was used to mass-produce SR-21. The biomass was increased from 1.12 g/L at Day 0 to 12 g/L at Day 4. We established optimal culture conditions of 5% glucose, 2% sea salt, and 1% yeast extract for SR-21. Three concentrations of dried fungal meal (0, 1, or 3% in the diet) were fed to birds over a 3-week period. There were no negative effects of 1 and 3% dietary SR-21 on egg production, egg weight, and egg yolk weight. The DHA content of yolk was increased by the dietary supplementation with the fungal meal both in the 1 and 3% treatments. Dietary fungal meal treatments increased the DHA concentrations of liver and plasma. However, dietary DHA enrichment had no effect on the expression of hepatic lipogenic genes in laying hens.

Effects of dietary algal docosahexaenoic acid oil supplementation on fatty acid deposition and gene expression in laying Leghorn hens

2004 ◽  
Vol 142 (6) ◽  
pp. 683-690 ◽  
Author(s):  
C. H. CHENG ◽  
T. F. SHEN ◽  
W. L. CHEN ◽  
S. T. DING
Keyword(s):  
Transcription Factors ◽  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Acid Deposition ◽  
Egg Production ◽  
Laying Hens ◽  
Regulatory Element ◽  
Egg Yolk ◽  
Total Fatty Acids ◽  
Egg Yolks

The purpose of the current study was to determine the effects of dietary docosahexaenoic acid (DHA) on fatty acid deposition in egg yolk and various tissues of laying Leghorn hens, and on the expression of hepatic lipogenesis-related transcription factors. There were three treatments with 10 Leghorn hens per treatment. Diets were based on maize-soybean meal (ME: 12 MJ/kg; CP: 171 g/kg; Ca: 34 g/kg) supplemented with 0 (plus 20 g butter/kg), 5 g (plus 15 g butter/kg) or 20 g algal DHA oil/kg. The egg production of the birds was not affected by dietary DHA oil (P>0·05). The DHA content in egg yolks of the laying hens increased significantly (P<0·01) with the dietary supplementation of DHA. The DHA content of the total fatty acids in the egg yolk of laying hens was 4±16, 11±43 and 20±90 g/kg for 0, 5 and 20 g/kg DHA treatments, respectively for the first week, and 6±16, 18±49 and 31±86 g/kg for the second week. Therefore, algal DHA oil can be utilized by laying Leghorn hens to enhance egg yolk DHA content and produce high DHA eggs. The concentrations of triacylglycerol (TG) and cholesterol in plasma of laying Leghorn hens were not affected by dietary DHA treatments (P>0·005). The DHA content in plasma and livers of laying hens was increased with the addition of DHA in the diet (P<0·05). The DHA content in the skeletal muscle of Leghorn hens was also increased with the increase in dietary DHA. The data indicate that dietary DHA oil can be incorporated into plasma, egg yolks and various tissues. The mRNA concentrations of the lipogenesis-related transcription factors, sterol regulatory element binding protein 1 (SREBP1) and SREBP2, in the livers of laying Leghorn hens were not affected by the supplement of algal DHA oil for 2 weeks, suggesting that the expressions of these transcription factors are tightly controlled and not sensitive to DHA treatments in laying hens.

scholarly journals 137 Dietary Supplementations of Ethanolamine and Serine Enhance Docosahexaenoic Acid Deposition in Eggs of Laying Hens

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 107-108
Author(s):  
Andrew D Magnuson ◽  
Xingen Lei
Keyword(s):  
Gene Expression ◽  
Docosahexaenoic Acid ◽  
Laying Hens ◽  
Total Phospholipid ◽  
Choline Chloride ◽  
Basal Diet ◽  
Egg Yolk ◽  
Analysis Data ◽  
Mrna Levels ◽  
Dha Enrichment

Abstract Enrichment of docosahexaenoic-acid (DHA) into eggs of laying-hens may be limited by the availability phospholipids as a deposition sink. The present study was to determine if dietary supplementations of phospholipid-component molecules or synthesis-enhancers: choline, serine, and ethanolamine could elevate phospholipid and DHA enrichment in the eggs and tissues of hens. A total of 50-White-Leghorn-Shavers (42-wk old) were individually caged and divided into 5 groups (n = 10/group). The 5 groups of hens were fed the following diets for 3 wk: Diet-1 = a corn soybean-meal basal-diet, Diet-2 = Diet-1 + 4%-microalgae (Aurantiochytrium, Heliae, Gibert, AZ, 1.81 g-DHA/kg) + choline-chloride (26.3 g/kg diet, 60% purity, DSM-Co., Basel, Switzerland), Diet-3 = Diet-2 + 1.41% of L-serine (100% purity, Ajinomoto-Co., Inc., Kawasaki, Japan), Diet-4 = Diet-2 + 100 mg of ethanolamine/kg (99% purity, Sigma-Aldirch-Co., St Loius, MO), and Diet-5 = Diet-3 + 100 mg of ethanolamine/kg. At the end of study, eggs, liver, ovary, and adipose samples were collected from 6 hens/group for biochemical analysis. Data were analyzed by one-way ANOVA. Compared with Diet-1, Diet-2 enhanced (P &lt; 0.05) DHA concentrations in egg yolk and liver by 213 mg/egg and 2.98 mg/g tissue, respectively, but decreased (P &lt; 0.05) total phospholipid-concentrations of yolk and liver by 50%, and hepatic-mRNA levels of elongases-2/5 and desaturases-4/6 by 25–50%. Compared with Diet-2, Diet-5 enhanced (P &lt; 0.05) DHA (by 20%) and phospholipid (by 40%) concentrations in the egg yolk, and upregulated (P &lt; 0.05) lipid-metabolism genes involved in the citicoline (CDP, up-to-3-fold) and CDP-ethanolamine (up-to-2.5-fold) pathways in the liver and ovary-tissue. In comparison, Diets-3 and 4 resulted in only 3–11% higher (P &lt; 0.05) DHA-concentrations in the liver over Diet-2. In conclusion, feeding hens a high DHA and choline diet enriched DHA in the egg yolk and down-regulated lipogenesis-gene-expression in the tissues. Supplementing the diet with extra-serine and ethanolamine further-enhanced the DHA enrichment in the egg yolk and restored the high DHA-mediated changes in the gene-expression. (Supported in part by DOE-MAGIC-grant DE-EE0007091, USDA-grant 2019-69012-29905, and Cornell-University-Hatch-grants NYC-127419/127302).

scholarly journals Effect of Dietary Incorporation of Linseed Alone or Together with Tomato-Red Pepper Mix on Laying Hens’ Egg Yolk Fatty Acids Profile and Health Lipid Indexes

Nutrients ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 813 ◽  
Author(s):  
Besma Omri ◽  
Raja Chalghoumi ◽  
Luana Izzo ◽  
Alberto Ritieni ◽  
Massimo Lucarini ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Laying Hens ◽  
Dietary Treatment ◽  
Egg Yolk ◽  
Red Pepper ◽  
Standard Diet ◽  
Fatty Acids Profile ◽  
Tomato Paste ◽  
Diet Control

This study evaluated the effect of linseed incorporation in laying hens’ feed (alone or along with a tomato-red pepper mix) on laying hens’ egg yolk fatty acids profile, as well as on their atherogenic (IA) and thrombogenic (IT) health lipid indexes, and the ratio between the hypocholesterolemic and hypercholesterolemic fatty acids (HH). Sixty 27 weeks-old Novogen White laying hens were divided into three groups and given 100 g/hen/day of a standard diet (Control, C) containing 4.5% of ground linseed (Linseed diet, L), containing 1% of dried tomato paste and 1% sweet red pepper (Lineseeds-Tomato-Pepper, LTP). The linseed dietary inclusion significantly reduced the egg yolk content of palmitic acid from 25.41% (C) to 23.43% (L) and that of stearic acid from 14.75% (C) to 12.52% (L). Feeding 4.5% ground linseed did not affect the egg yolk content of α-Linolenic acid but significantly increased the egg yolk concentration of eicosapentaenoic acid (EPA) from 0.011% (C) to 0.047% (L) and that of docosahexaenoic acid (DHA) from 1.94% (C) to 2.73% (L). The IA and the HH were not affected (p > 0.05) by the dietary addition of linseed, whereas the IT decreased (p < 0.05) from 1.16 (C) to 0.86 (L). Adding tomato-sweet red pepper mix to the linseed-supplemented feed did not affect the measured parameters as compared to the linseed dietary treatment.

scholarly journals Time course of nutritional and functional property changes in egg yolk from laying hens fed docosahexaenoic acid-rich microalgae

2020 ◽  
Vol 99 (9) ◽  
pp. 4616-4625 ◽  
Author(s):  
Bing Liu ◽  
Qin Zhou ◽  
Jiaming Zhu ◽  
Gang Lin ◽  
Dongyou Yu ◽  
...  
Keyword(s):  
Docosahexaenoic Acid ◽  
Functional Property ◽  
Time Course ◽  
Laying Hens ◽  
Egg Yolk ◽  
Property Changes

scholarly journals Production of laying hens in different rearing systems under hot weather

2017 ◽  
Vol 40 ◽  
pp. 37677
Author(s):  
Daniel Araujo Netto ◽  
Héder José D'Ávila Lima ◽  
Julia Rodrigues Alves ◽  
Bianca Corrêa de Morais ◽  
Mauricio Silva Rosa ◽  
...  
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Production Systems ◽  
Egg Yolk ◽  
Feed Conversion ◽  
Negative Effects ◽  
External Quality ◽  
Weight Variation ◽  
Randomized Design

 The environment is very important for the performance of laying hens; thus, techniques are required to improve production systems, providing better welfare for poultry and consequent increase in the quality of the final product, the egg. This study aimed to evaluate the effects of rearing system, on the floor and in cage, on the performance and egg internal and external quality of laying hens. A total of 320 Hysex Brown laying hens, with 34-43 weeks days of age, was distributed in a completely randomized design, with two treatments, floor and cage, with 10 replicates each. The parameters evaluated were: individual feed intake, egg production per replicate, feed conversion per dozen eggs, egg weight; weight and percentage participation of shell, albumen and yolk, specific gravity, body weight variation and viability of birds. The results show that the birds raised on the floor showed best results as to egg production, besides the best internal and external quality, with greater weight of egg, yolk, albumen, shell and with lower losses of eggs. Because of the negative effects of the cage system, in general, hens presented lower results when compared to results of those raised on the floor. 

scholarly journals Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth

2021 ◽  
Vol 11 ◽  
Author(s):  
Takahiro Nii ◽  
Takashi Bungo ◽  
Naoki Isobe ◽  
Yukinori Yoshimura
Keyword(s):  
Gene Expression ◽  
Oral Administration ◽  
Blood Plasma ◽  
Sodium Sulfate ◽  
Egg Production ◽  
Laying Hens ◽  
Ovarian Follicle ◽  
Egg Yolk ◽  
Dextran Sodium Sulfate ◽  
Intestinal Environment

Intestinal environments such as microbiota, mucosal barrier function, and cytokine production affect egg production in laying hens. Dextran sodium sulfate (DSS) is an agent that disrupts the intestinal environment. Previously, we reported that the oral administration of dextran sodium sulfate (DSS: 0.9 g/kg BW) for 5 days caused severe intestinal inflammation in laying hens. However, the DSS concentration in the previous study was much higher to induce a milder disruption of the intestinal environment without heavy symptoms. Thus, the goal of this study was to determine the effects of a lower dose of DSS on the intestinal environment and egg production in laying hens. White Leghorn laying hens (330-day old) were oral administered with or without 0.225 g DSS/kg BW for 28 days (DSS and control group: n = 7 and 8, respectively). Weekly we collected all laid eggs and blood plasma samples. Intestinal tissues, liver, ovarian follicles, and the anterior pituitary gland were collected 1 day after the final treatment. Lower concentrations of orally administered DSS caused (1) a decrease in the ratio of villus height/crypt depth, occludin gene expressions in large intestine and cecal microbiota diversity, (2) a decrease in egg yolk weight, (3) an increase in VLDLy in blood plasma, (4), and enhanced the egg yolk precursor accumulation in the gene expression pattern in the follicular granulosa layer, (5) an increase in FSH and IL-1β gene expression in the pituitary gland, and (6) an increase in concentration of plasma lipopolysaccharide binding protein. These results suggested that the administration of the lower concentration of DSS caused a slight disruption in the intestinal environment. This disruption included poor intestinal morphology and decreased cecal microbiome diversity. The change in the intestinal environment decreases egg yolk size without decreasing the VLDLy supply from the liver. The decrease in egg yolk size is likely to be caused by the dysfunction of egg-yolk precursor uptake in ovarian follicles. In conclusion, the oral administration of a lower dose of DSS is an useful method to cause slight disruptions of intestinal environment, and the intestinal condition decreases egg yolk size through disfunction of ovarian follicle.

scholarly journals Extraction of Uronic Acid From Sargassum crassifolium and Its Feeding Effects On The Eggs Production and Quality of Lohmann Chicken Eggs

2020 ◽  
Vol 7 (3) ◽  
pp. 264
Author(s):  
Veybe Gresje Kereh ◽  
Ivonne Maria Untu ◽  
Marie Najoan ◽  
T. Lumi
Keyword(s):  
Drinking Water ◽  
Uronic Acid ◽  
Egg Production ◽  
Pathogenic Bacteria ◽  
Laying Hens ◽  
Brown Seaweed ◽  
Egg Yolk ◽  
Egg Mass ◽  
Chicken Eggs

ABSTRACTThe use of antibiotics in chicken feed is currently banned because it can cause resistance to pathogenic bacteria and cause residues in products. This study aimed to see the effect of drinking water containing uronic acid extracted from Sargassum crassifolium (S. crassifolium) on the physical and chemical quality of Lohmann chicken eggs. One hundred and twenty laying hens were divided into 2 groups: (1) chickens given commercial feed containing antibiotics and (2) feed without additional antibiotics. The chickens were randomly assigned to one of the 5 brown seaweed supplementation treatments in drinking water A1=0.0% S.crassifolium (control); A2=2.5% S.crassifolium; A3=5.0% S.crassifolium; A4=7.5% S.crassifolium; A5=10.0% S.crassifolium. The study used a completely randomized factorial design of 5 treatments, 2 factors, and 3 replications. Each replication consisted of 6 heads of laying hens. There were no differences between treatments on the performance of laying hens (egg production, egg weight, egg mass, feed conversion); physical quality (eggshell, egg yolk, egg white, shell thickness, egg yolk, Haugh unit, egg index) and chemistry (superoxide dismutase production, thiobarbituric acid reactive substances (TBARS) and Lohmann chicken egg cholesterol) but there was a difference in feed intake. The uronic acid extracted from S. crassifolium has not been able to increase the production and quality of Lohmann chicken eggs.Keywords: lohmann chicken, quality of eggs, Sargassum crassifolium, uronic acid

scholarly journals Effectivity of BS4 enzyme complex on the performance of laying hens fed with different ingredients

2016 ◽  
Vol 21 (1) ◽  
pp. 1
Author(s):  
Arnold P. Sinurat ◽  
Tresnawati Purwadaria ◽  
Tuti Haryati
Keyword(s):  
Rice Bran ◽  
Egg Production ◽  
Laying Hens ◽  
Palm Kernel ◽  
Egg Yolk ◽  
P Value ◽  
Enzyme Complex ◽  
Recorded Data ◽  
Egg Shell ◽  
Digestible Amino Acids

<p class="abstrak2">An experiment was carried out to evaluate the effectivity of an enzyme complex produced by Eupenicilium javanicum BS4 on the performance of laying hens fed with different feed ingredients. Three diets were formulated with similar nutrients (protein, ME, digestible amino acids, Ca and available P) value to meet the nutrient requirement of laying hens.  Diets were formulated based on maize, palm kernel cake (PKC) or rice bran.  The diets were either supplemented or not with BS4 enzyme complex and fed to Isa Brown pullet from 19 to 37 weeks old. Each diet was fed to 24 birds and the performances were recorded. Data were analysed statistically with 2 X 3 factorial design with six replicates. Results showed that supplementation of BS4 enzyme into laying hens diet did not influence body weight change, feed intake, mortalities, egg weight and egg shell thickness but significantly increased egg production (P&lt;0.05) and the FCR (P&lt;0.01) in any feedstuff tested. It also improved egg yolk colour score on diet contained 20% PKC. Laying hens fed 20% PKC produced more eggs than those fed maize or rice bran. The egg shell of laying hens fed rice bran were thicker than those fed with maize or PKC. It is concluded that BS4 enzyme complex is effective when supplemented into laying hens diet.</p>

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