Skin deiodinase profiles and associated patterns of hair follicle activity in cashmere goats of contrasting genotypes

2004 ◽  
Vol 55 (4) ◽  
pp. 443 ◽  
Author(s):  
S. M. Rhind ◽  
C. E. Kyle
Keyword(s):  
Hair Follicle ◽  
Physiological State ◽  
Genotypic Differences ◽  
Type Ii ◽  
Long Day ◽  
Significant Difference ◽  
Igf I ◽  
Fibre Growth ◽  
Cashmere Goats ◽  
Skin Samples

The roles of skin deiodinase enzymes in the expression of genotypic differences in hair follicle activity and cashmere fibre growth and moult were studied in 9 Siberian (S) and 10 Scottish cashmere (SC) goats. The proportion of animals exhibiting cashmere growth was significantly greater in S than in SC goats in both late July (P < 0.001; early in the season of growth) and between January and April (P < 0.001; late in the season of growth), but there was no significant difference in the time of moult onset. Patterns of hair follicle activity exhibited similar seasonal differences, with secondary follicle activity being higher in S than in SC animals during the January–June period. Overall mean concentrations of IGF-I were lower (P < 0.01) in S than in SC animals and mean T4 concentrations were higher (P < 0.05) in S than in SC animals, but these differences were not related to genotypic differences in follicle activity or cashmere fibre growth. Mean concentrations of insulin, prolactin, and T3 did not differ with genotype. Between January and early April, mean prolactin concentrations were higher in S than in SC animals (P < 0.05). Monodeiodinase type II (MDII) activity in skin samples was highest (P < 0.001) during December and January (short day photoperiod) and was lower in S than in SC animals (P < 0.05) at these times. Mean monodeiodinase type III (MDIII) activity was highest during June and July (long day photoperiod). There was no difference between genotypes in the overall mean levels of deiodinase activity but, during January–March, when prolactin concentrations were higher in S than in SC goats, there was a higher rate of follicle activity and fibre growth in S goats. It is postulated that individual and genotype differences in hair follicle activity and cashmere growth are partially dependent on the pattern of expression of deiodinase enzymes in the skin. However, the actions of each enzyme are likely to depend on, among other factors, the absolute levels of expression of each type, the ratio of MDIII to MDII, the physiological state of the follicle, and the associated hormone profiles.

The effects of bovine somatotropin on hair follicle activity and cashmere fibre growth in goats

1999 ◽  
Vol 50 (8) ◽  
pp. 1365 ◽  
Author(s):  
D. Villar ◽  
S. M. Rhind ◽  
S. R. McMillen ◽  
P. Dicks
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Hair Follicle ◽  
Bovine Somatotropin ◽  
Igf I ◽  
Fibre Growth ◽  
Cashmere Goats ◽  
The Mean ◽  
Secondary Fibre

The aim of this study was to determine the role of circulating growth hormone (GH) profiles in the cessation of secondary hair follicle activity and secondary fibre growth in goats during autumn/winter and to determine whether it is possible to extend the period of growth of this fibre by artificially increasing circulating concentrations of this hormone. Nine cashmere goats were each injected, weekly, with 50 mg of bovine somatotropin (bST; treated) in carrier oil, for a period of 5 months between late August and late January. Goats of a second group of 9 animals were injected with sesame oil and served as controls. Treated goats had significantly higher circulating concentrations of growth hormone (P< 0.001), insulin (P< 0.01), thyroxine (P< 0.001), and insulin-like growth factor-I (IGF-I; P< 0.001). Secondary fibre growth rate and liveweight gains were significantly (P< 0.05) higher in treated than control goats. The proportion of active secondary follicles was positively associated with circulating IGF- I concentrations in treated but not control goats. Follicle activity was not related to the profiles of any of the other hormones measured. The mean date of cessation of secondary fibre growth was not affected by treatment. It is concluded that treatment with bST stimulated the rate of secondary fibre growth but did not delay the time of cessation of this growth or the decline in follicle activity. It is further concluded that circulating hormone profiles do not directly control follicle activity and secondary fibre growth and moult and that their effects probably involve changes within the skin and/or hair follicle, possibly in hormone receptor populations, deiodinase enzyme activity, or growth factor synthesis.

An investigation of the role of thyroid hormones in the control of secondary fibre growth and moult in cashmere goats

1995 ◽  
Vol 1995 ◽  
pp. 104-104
Author(s):  
S.M. Rhind ◽  
S.R. McMillen
Keyword(s):  
Seasonal Variation ◽  
Thyroid Hormones ◽  
Hair Follicle ◽  
Seasonal Cycle ◽  
Reproductive Activity ◽  
Direct Role ◽  
Fibre Growth ◽  
Cashmere Goats ◽  
Secondary Fibre

The growth of a fine undercoat (cashmere) in goats is a seasonal phenomenon; growth generally begins during the autumn, continues into the winter and is followed by a moult in the spring. The endocrine mechanisms involved in the control of these processes are unclear but the thyroid hormones have been implicated in the control of fibre growth (Ferguson, Schenckel, Carter and Clarke, 1956; Lincoln, Klandorf and Anderson, 1980) and in the normal seasonal cycle of reproductive activity in sheep (Follett and Potts, 1990). However, it is not known whether or not the thyroid hormones have a role in the mediation of photoperiodic effects on secondary hair follicle activity in goats.Most of the metabolic activity of the thyroid hormones is thought to be attributable to triiodothyronine (T3) which is primarily derived from thyroxine (T4) by a process of monodeiodination. This process can be inhibited by treatment with methylthiouracil.The aim of this study was to suppress the synthesis of T3 and so to determine whether or not it has a direct role in the control of seasonal variation of secondary hair follicle activity and cashmere growth and moulting.

Effects of season and of manipulation of circulating prolactin concentrations on deiodinase activity in cashmere goat skin

2004 ◽  
Vol 55 (2) ◽  
pp. 211 ◽  
Author(s):  
S. M. Rhind ◽  
C. E. Kyle ◽  
E. I. Duff
Keyword(s):  
Seasonal Changes ◽  
Activity Levels ◽  
Type Ii ◽  
Deiodinase Activity ◽  
Fibre Growth ◽  
Cashmere Goats ◽  
High Concentrations ◽  
High Degree ◽  
Very High ◽  
Synthesis And Degradation

The effects of season and of manipulations of circulating prolactin concentrations on the expression of monodeiodinase type II (MDII) and monodeiodinase type III (MDIII) expression in the skin of cashmere goats were investigated. Deiodinase enzymes control the rates of synthesis and degradation of triiodothyronine (T3), a hormone implicated in the control of hair follicle activity, and so changes in expression with season or prolactin profiles may affect cashmere fibre growth and moult. There was a very high degree of variation between animals in expression of each enzyme. There was also evidence of seasonal variation in expression within individuals, with a trend towards lower levels of activity of MDII during periods of long daylength (July and April) and highest levels in December, whereas levels of MDIII were highest in July and lowest in December. Collectively, these differences with season resulted in significant changes with date in the MDIII/MDII ratio. It is concluded that the seasonal changes in deiodinase activity, and in particular in the MDIII/MDII ratio, may have an important role in the regulation of follicle activity. Levels of MD activity were poorly correlated with circulating hormone profiles. Nevertheless, since manipulation of the circulating concentrations of prolactin induced additional changes in MDIII/MDII ratios, which were associated with treatment differences in follicle activity or moult, it is concluded that seasonal changes in prolactin may determine patterns of enzyme activity, with high concentrations of prolactin being associated with reduced net levels of T3 synthesis.

An investigation of the role of thyroid hormones in the control of secondary fibre growth and moult in cashmere goats

1995 ◽  
Vol 1995 ◽  
pp. 104-104
Author(s):  
S.M. Rhind ◽  
S.R. McMillen
Keyword(s):  
Seasonal Variation ◽  
Thyroid Hormones ◽  
Hair Follicle ◽  
Seasonal Cycle ◽  
Reproductive Activity ◽  
Direct Role ◽  
Fibre Growth ◽  
Cashmere Goats ◽  
Secondary Fibre

The growth of a fine undercoat (cashmere) in goats is a seasonal phenomenon; growth generally begins during the autumn, continues into the winter and is followed by a moult in the spring. The endocrine mechanisms involved in the control of these processes are unclear but the thyroid hormones have been implicated in the control of fibre growth (Ferguson, Schenckel, Carter and Clarke, 1956; Lincoln, Klandorf and Anderson, 1980) and in the normal seasonal cycle of reproductive activity in sheep (Follett and Potts, 1990). However, it is not known whether or not the thyroid hormones have a role in the mediation of photoperiodic effects on secondary hair follicle activity in goats.Most of the metabolic activity of the thyroid hormones is thought to be attributable to triiodothyronine (T3) which is primarily derived from thyroxine (T4) by a process of monodeiodination. This process can be inhibited by treatment with methylthiouracil.The aim of this study was to suppress the synthesis of T3 and so to determine whether or not it has a direct role in the control of seasonal variation of secondary hair follicle activity and cashmere growth and moulting.

scholarly journals The Overexpression of Tβ4 in the Hair Follicle Tissue of Alpas Cashmere Goats Increases Cashmere Yield and Promotes Hair Follicle Development

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 75 ◽  
Author(s):  
Bai Dai ◽  
Hao Liang ◽  
Dong-dong Guo ◽  
Zhao-wei Bi ◽  
Jian-long Yuan ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Hair Follicles ◽  
Erk Signaling ◽  
Type Ii ◽  
Thymosin Beta 4 ◽  
The Past ◽  
Extracellular Signal ◽  
Cashmere Goats ◽  
Hair Follicle Development

Increased cashmere yield and improved quality are some goals of cashmere goat breeding. Thymosin beta-4 (Tβ4) plays a key role in the growth and development of hair follicles. For the past ten years, we have evaluated the role of Tβ4 by establishing a flock of 15 cashmere goats that specifically overexpress the Tβ4 gene in the hair follicles. These Tβ4 overexpression (Tβ4-OE) cashmere goats had more secondary hair follicles than the WT goats and produced more cashmere. Meanwhile, combined analysis of the skin transcriptome and proteome in cashmere goats suggested that Tβ4 may affect hair growth by interacting with keratin type II cytoskeletal 4 epidermal (KRT4) to mediate the extracellular signal-regulated protein kinase (ERK) signaling pathway, thereby promoting the development of secondary hair follicles, and consequently, increasing cashmere yield. Thus, the specific overexpression of Tβ4 in the hair follicles of cashmere goats effectively increased the cashmere yield.

scholarly journals Analysis of sagittal profile and radiographic parameters in symptomatic thoracolumbar disc herniation patients

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Ang Gao ◽  
Yongqiang Wang ◽  
Miao Yu ◽  
Xiaoguang Liu
Keyword(s):  
Disc Herniation ◽  
Thoracic Spine ◽  
Normal Population ◽  
Sagittal Profile ◽  
Sacral Slope ◽  
Type I ◽  
Type Ii ◽  
Radiographic Parameters ◽  
Significant Difference ◽  
Thoracolumbar Region

Abstract Background Few studies describe thoracolumbar disc herniation (TLDH) as an isolated category, it is frequently classified as the lower thoracic spine or upper lumbar spine. Thus, less is known about the morphology and aetiology of TLDH compared to lumbar disc herniation (LDH). The aim of study is to investigate sagittal alignment in TLDH and analyze sagittal profile with radiographic parameters. Methods Data from 70 patients diagnosed with TLDH were retrospectively reviewed. The thoracic-lumbar alignment was depicted by description of curvatures (the apex of lumbar curvature, the apex of thoracic curvature, and inflexion point of the two curvatures) and radiographic parameters from complete standing long-cassette spine radiographs. The rank sum test was utilised to compare radiographic parameter values in each subtype. Results We found two subtypes differentiated by the apex of thoracic kyphotic curves. The sagittal profile was similar to that of the normal population in type I, presenting the apex of the thoracic kyphotic curve located in the middle thoracic spine. The well aligned thoracic-lumbar curve was disrupted in type II, presenting the apex of the thoracic kyphotic curve located in the thoracolumbar region in type II patients. Thirty-six patients were classified as type I, and 34 patients were classified as type II. The mean sagittal vertical axis, T1 pelvic angle and L1 pelvic angle were 27.9 ± 24.8°, 8.2 ± 7.3° and 6.2 ± 4.9°, respectively. There was significant difference (p < 0.001) of thoracolumbar angle between type I (14.9 ± 7.9°) and type II patients (29.1 ± 13.7°). Conclusions We presented two distinctive sagittal profiles in TLDH patients, and a regional kyphotic deformity with a balanced spine was validated in both subtypes. In type I patients, disc degeneration was accelerated by regional kyphosis in the thoracolumbar junction and eventually caused disc herniation. In type II patients, excessive mechanical stress was directly loaded at the top of the curve (thoracolumbar apex region) rather than being diverted by an arc as in a normal population or type I patients. Mismatch between shape and sacral slope value was observed, and better agreement was found in Type II patients.

scholarly journals Proximal Gastrectomy versus Total Gastrectomy for Siewert Type II Adenocarcinoma of the Esophagogastric Junction: A Comprehensive Analysis of Data from the SEER Registry

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Kaixuan Zhu ◽  
Yingying Xu ◽  
Jiaxin Fu ◽  
Farah Abdidahir Mohamud ◽  
Zongkui Duan ◽  
...  
Keyword(s):  
Total Gastrectomy ◽  
Surgical Approach ◽  
Esophagogastric Junction ◽  
Proximal Gastrectomy ◽  
Locally Advanced ◽  
Cox Proportional Hazards Model ◽  
Type Ii ◽  
Siewert Type ◽  
Siewert Type Ii ◽  
Significant Difference

Background. To determine the ideal surgical approach (total gastrectomy (TG) vs. proximal gastrectomy (PG)) for Siewert type II adenocarcinoma of the esophagogastric junction (AEG), we searched and analyzed the Surveillance, Epidemiology, and End Results (SEER) data. Methods. Patients with Siewert type II AEG treated by TG or PG were identified from the 2004–2014 SEER dataset. We obtained the patients’ overall survival (OS) and cancer-specific survival (CSS) and stratified the patients by surgical approach. We performed a propensity score 1 : 1 matching (PSM) analysis and a univariate and multivariate Cox proportional hazards model. Results. A total of 2,217 patients with 6th AJCC stage IA–IIIB Siewert type II AEG was examined: 1,584 patients (71.4%) underwent PG, and 633 patients (28.6%) underwent TG. The follow-up time was 1–131 months. OS favored total gastrectomy before the PSM analysis (χ2=3.952, p=0.047), but after this analysis, there was no significant difference between TG and PG (χ2=2.227, p=0.136). The univariate and multivariate analyses identified age as an independent factor, and an X-tail analysis revealed 70 years as a cut-off point. The patients aged≥70 years obtained a significant long-term OS benefit from PG compared to TG (χ2=8.245, p=0.004), and those aged<70 years showed no difference between TG and PG (χ2=0.167, p=0.682). Conclusions. PG showed an equivalent survival benefit to TG in both the early and locally advanced stages of Siewert type II AEG. For elderly patients, PG is strongly recommended because of its clearer OS benefit compared to TG.

scholarly journals MK-677, an Orally Active Growth Hormone Secretagogue, Reverses Diet-Induced Catabolism1

1998 ◽  
Vol 83 (2) ◽  
pp. 320-325 ◽  
Author(s):  
M. G. Murphy ◽  
L. M. Plunkett ◽  
B. J. Gertz ◽  
W. He ◽  
J. Wittreich ◽  
...  
Keyword(s):  
Placebo Group ◽  
Caloric Restriction ◽  
Nitrogen Balance ◽  
Binding Protein ◽  
Growth Hormone Secretagogue ◽  
Significant Difference ◽  
Igf I ◽  
Igf Binding ◽  
Igf Binding Protein ◽  
Orally Active

The reversal of diet-induced negative nitrogen balance by GH suggests a possible therapeutic role for GH treatment in catabolic patients. A double-blind, randomized, placebo-controlled, two-period, cross-over study was designed to investigate whether MK-677, an orally active nonpeptide mimic of GH-releasing peptide, can reverse diet-induced protein catabolism. Eight healthy volunteers (ages 24–39 yr) were calorically restricted (18 kcal/kg·day) for two 14-day periods. During the last 7 days of each diet period, subjects received either oral MK-677 25 mg or placebo once daily. There was a 14- to 21-day washout interval between periods. During the first week of caloric restriction (i.e. diet alone), daily nitrogen losses were similar for both treatment groups (mean ± se; MK-677 group −2.67 ± 0.40 g/day vs. placebo group− 2.83 ± 0.26 g/day). During the second week (diet and study drug), mean daily nitrogen balance was 0.31 ± 0.21 g/day in the MK-677 treatment group compared with −1.48 ± 0.21 g/day in the placebo group (P &lt; 0.01). MK-677 improved nitrogen balance integrated over the 7 days of treatment; area under the curve day 8–14 nitrogen balance response was +2.69 ± 5.0 (se) for MK-677 and −8.97 ± 5.26 g·day for placebo (P &lt; 0.001). MK-677 produced a peak GH response of 55.9 ± 31.7 μg/L after single dose (day 1 of treatment) and 22.6 ± 9.3 μg/L after a week of dosing compared with placebo treatment peak GH values of approximately 9 (treatment day 1) and approximately 7 μg/L (treatment day 7). Following the initial 7-day caloric restriction, insulin-like growth factor-I (IGF-I) declined from 232 ± 25 to 186 ± 19 ng/mL in the MK-677 group and from 236 ± 19 to 174 ± 23 ng/mL in the placebo group. Mean IGF-I concentration increased significantly during MK-677 to 264 ± 31 ng/mL (mean for the last 5 days of treatment) compared with 188 ± 19 ng/mL with placebo (P &lt; 0.01). No significant difference in IGF binding protein-2 was found between the MK-677 and placebo treatments. However, the mean in IGF binding protein-3 for the last 5 days of MK-677 treatment was also significantly increased to 3273 ± 330 ng/mL (mean ± se) compared with placebo 2604 ± 253 ng/mL (P &lt; 0.01). Neither the serum cortisol nor the PRL response was significantly greater after 7 days of MK-677 dosing compared with 7 days of placebo. MK-677 (25 mg) was generally well tolerated and without clinically significant adverse experiences. In conclusion, MK-677 reverses diet-induced nitrogen wasting, suggesting that if these short-term anabolic effects are maintained in patients who are catabolic because of certain acute or chronic disease states, it may be useful in treating catabolic conditions.

The nutritional biochemistry of wool and hair follicles

2000 ◽  
Vol 70 (2) ◽  
pp. 181-195 ◽  
Author(s):  
P.I. Hynd
Keyword(s):  
Amino Acids ◽  
Vitamin D ◽  
Amino Acid ◽  
Vitamin A ◽  
Hair Follicle ◽  
Significant Activity ◽  
Direct Effects ◽  
Outer Root Sheath ◽  
Root Sheath ◽  
Fibre Growth

AbstractThe rôle of various classes of nutrients (energy substrates, vitamins, minerals, amino acids) in the production of wool and hair from follicles, is considered for a variety of animal species. The wool and hair follicle have evolved a number of interesting features of carbohydrate metabolism including glutaminolysis, aerobic glycolysis, significant activity of the pentose phosphate pathway, and storage and mobilisation of glycogen. Presumably the necessity to continue to produce fibre despite fluctuations in the supply of oxygen and nutrients has resulted in some of these unique features, while others reflect the high level of DNA and protein synthesis occurring in the follicle. While it is considered that energy does not normally limit fibre growth, the relative contributions of aerobic and anerobic metabolism will greatly influence the amount of ATP available for follicle activity, such that energy availability may at times alter fibre growth. Alopecia and deficient fibre growth are consistent outcomes of deficiencies of biotin, riboflavin, pyridoxine, folate and pantothenic acid, but the precise rôles of these vitamins in follicle function await elucidation. Folate, in particular appears to play an important rôle in wool production, presumably reflecting its involvement in methionine metabolism. Cholecalciferol (vitamin D) significantly alters fibre growth in cultured follicles; vitamin D receptors are located in the outer root sheath, bulb, and dermal papilla of the follicle; and alopecia occurs in humans with defects in the vitamin D receptor. Retinol (vitamin A), too, appears to influence follicle function by altering keratinocyte proliferation and differentiation, with direct effects on the expression of keratin genes. The receptors for the retinoids are present in the keratogenous zone, the outer root sheath, the bulb, and the sebaceous glands. Vitamin A may also act indirectly on follicle function by influencing the activity of the insulin-like and epidermal growth factors and by altering vitamin D activity. At present there is little evidence implicating alpha-tocopherol (vitamin E) or phytylmenaquinone (vitamin K) in follicular events. Of the minerals, only copper and zinc have been shown to have direct effects on follicle function, independent of effects on food intake. Copper has direct effects on the activity of an unidentified enzyme on oxidation of thiol groups to form disulphide linkages. Wool produced by copper-deficient sheep lacks crimp, is weak and lustrous. Copper is also necessary for the activity of tyrosinase and the tyrosinase-related proteins involved in melanin synthesis. Zinc, like copper, is required for the normal keratinization of fibres but again, the precise rôle has yet to be elucidated. While the importance of amino acid supply for wool growth has long been established, there are still some unaswered questions such as; what are the effects of amino acids on fibre growth in animals other than sheep; what are the characteristics of the amino acid transport genes and proteins operating in the wool and hair follicle; and what are the specific rôles for amino acids in follicle function.

Retrospective Study on Hospital Admissions due to Pyelonephritis in Type II Diabetic Patients

2021 ◽  
Vol 5 (6) ◽  
Author(s):  
Dilraj Dhillon ◽  
Thomas Randall ◽  
David Zezoff ◽  
Mouchumi Bhattacharyya
Keyword(s):  
Retrospective Study ◽  
Length Of Stay ◽  
Hospital Admissions ◽  
Type Ii Diabetes Mellitus ◽  
Hospitalized Patients ◽  
Diabetic Patients ◽  
Clean Intermittent Catheterization ◽  
Type Ii ◽  
Indwelling Catheters ◽  
Significant Difference

Background: Pyelonephritis is a urinary tract infection that ascends to involve the kidneys. It can also occur as an infection secondary to bacteremia. Some pathogens that commonly cause pyelonephritis are E. coli, enterobacteriaceae, staphylococci, and pseudomonas. The initial patient presentation usually involves fever, chills, nausea, vomiting, costovertebral angle tenderness, and flank pain. Other cystitis symptoms such as dysuria, increased urinary frequency, malodorous urine, and hematuria may or may not be present. Symptoms of pyelonephritis with bacteriuria are sufficient for the diagnosis of pyelonephritis. Aim: The aim of this study was to investigate a potential link between Type II Diabetes Mellitus and pyelonephritis. Methods: In this retrospective study, hospitalized patients during the study period were reviewed. Variables examined were sex, age, and length of stay. Patients were excluded if they had known urogenital abnormalities, indwelling catheters (Foley, nephrostomy, suprapubic, or who regularly perform clean intermittent catheterization), were pregnant, or were on dialysis. Results: Of 333 patients analyzed, diabetics had a longer length of stay then non-diabetics (4.49 vs 3.67 days respectively; p=0.0041) and females were significantly younger than men in hospitalized patients for pyelonephritis were (50.0 vs 63.5 years; p=<0.0001). Further, it was found that diabetics were significantly older than nondiabetics were (60.4 vs 47.3; p=<0.0001) and more diabetics getting admitted with pyelonephritis were men vs women (59.32% vs 35.27%; p=0.0007). Conclusion: Results of the study were significant in showing that of all pyelonephritis-hospitalized patients on average the length of stay was longer for diabetics and it demonstrated that female patients with pyelonephritis are significantly younger than male patients hospitalized with pyelonephritis. Of note, there was no significant difference in the length of stay for diabetic patients based on their treatment modality (diet controlled vs. oral medications vs. insulin dependent vs. combined). The study also showed that diabetics getting admitted for pyelonephritis are more men and older in age compared to the nondiabetics.

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