Genetics of stripe rust resistance in 'Karamu' wheat (Triticum aestivum L.)

2005 ◽  
Vol 56 (6) ◽  
pp. 619 ◽  
Author(s):  
M. Imtiaz ◽  
M. G. Cromey ◽  
J. G. Hampton ◽  
F. C. Ogbonnaya
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Puccinia Striiformis ◽  
Triticum Aestivum L ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Dh Lines ◽  
Disease Pressure

The New Zealand wheat (Triticum aestivum L.) cv. Karamu (same parentage as the US cv. Anza) was originally believed to carry gene Yr18, which provides adult plant resistance to stripe rust (Puccinia striiformis f. sp. tritici), in addition to the seedling resistance gene YrA. Following the detection of virulence to the stripe rust resistance gene YrA in 1995, much of the resistance of Karamu was eroded and the cultivar suffered from occasional severe stripe rust outbreaks. This meant that either one or more new races of Puccinia striiformis f. sp. tritici with virulence to Yr18 had developed, or that Yr18 conferred inadequate resistance under high disease pressure. Karamu was crossed with cv. Otane, which carries Yr18, and 140 double haploid (DH) lines obtained from the F1 progeny were evaluated for seedling and adult plant resistance under greenhouse and field conditions. Evaluation of F1 plants against stripe rust pathotype 106E139A+ revealed that the resistance was recessive and that none of the resistance genes present was effective at the seedling stage. Segregation in the DH lines at the adult plant stage indicated that the resistance measured through infection type in both the greenhouse and the field was based on 3 genes, 1 from Karamu and 2 from Otane. However, the resistance gene from Karamu did not contribute towards resistance measured through final disease severity, but acted additively with genes from Otane in providing slow-rusting resistance as expressed by lower values for area under the disease progress curve. It was concluded that Karamu does not have gene Yr18, but rather possesses a recessive minor gene, which explains its adult plant susceptibility under high disease pressure. However, this Karamu gene did interact with Otane resistance genes to provide increased resistance.

scholarly journals Genome-wide Mapping for Stripe Rust Resistance Loci in Common Wheat Cultivar Qinnong 142

Plant Disease ◽  
2019 ◽  
Vol 103 (3) ◽  
pp. 439-447 ◽  
Author(s):  
Qingdong Zeng ◽  
Jianhui Wu ◽  
Shengjie Liu ◽  
Xianming Chen ◽  
Fengping Yuan ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Cultivar ◽  
Infection Type ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars

Stripe rust caused by Puccinia striiformis f. sp. tritici threatens worldwide wheat production. Growing resistant cultivars is the best way to control this disease. Chinese wheat cultivar Qinnong 142 (QN142) has a high level of adult-plant resistance to stripe rust. To identify quantitative trait loci (QTLs) related to stripe rust resistance, we developed a recombinant inbred line (RIL) population from a cross between QN142 and susceptible cultivar Avocet S. The parents and 165 F6 RILs were evaluated in terms of their stripe rust infection type and disease severity in replicated field tests with six site-year environments. The parents and RILs were genotyped with single-nucleotide polymorphism (SNP) markers. Four stable QTLs were identified in QN142 and mapped to chromosome arms 1BL, 2AL, 2BL, and 6BS. The 1BL QTL was probably the known resistance gene Yr29, the 2BL QTL was in a resistance gene-rich region, and the 2AL and 6BS QTLs might be new. Kompetitive allele specific polymerase chain reaction markers developed from the SNP markers flanking these QTLs were highly polymorphic in a panel of 150 wheat cultivars and breeding lines. These markers could be used in marker-assisted selection for incorporating the stripe rust resistance QTL into new wheat cultivars.

scholarly journals Genetic Relationship of Stripe Rust Resistance Genes Yr34 and Yr48 in Wheat and Identification of Linked KASP Markers

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 413-420 ◽  
Author(s):  
N. Qureshi ◽  
H. S. Bariana ◽  
P. Zhang ◽  
R. McIntosh ◽  
U. K. Bansal ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Genotypes ◽  
Stripe Rust Resistance Gene ◽  
Australian Continent

The Australian continent was free from wheat stripe rust caused by Puccinia striiformis f. sp. tritici until exotic incursions occurred in 1979 and 2002. The 2002 incursion enabled the identification of a new stripe rust resistance gene (Yr34) in the advanced breeding line WAWHT2046. In this study, we developed and validated markers closely linked with Yr34, which is located in the distal region in the long arm of chromosome 5A. Four kompetitive allele-specific polymerase chain reaction (KASP) and three sequence-tagged site (STS) markers derived from the International Wheat Genome Sequencing Consortium RefSeq v1.0 scaffold-77836 cosegregated with Yr34. Markers sun711, sun712, sun725, sunKASP_109, and sunKASP_112 were shown to be suitable for marker-assisted selection in a validation panel of 71 Australian spring wheat genotypes, with the exception of cultivar Orion that carried the Yr34-linked alleles for sunKASP_109 and sunKASP_112. Markers previously reported to be linked with adult plant stripe rust resistance gene Yr48 also cosegregated with Yr34. Wheat genotypes carrying Yr34 and Yr48 produced identical haplotypes for the Yr34-linked markers identified in this study and those previously reported to be linked with Yr48. Phenotypic testing of genotypes carrying Yr34 and Yr48 showed that both genes conferred similar seedling responses to pre-2002 and post-2002 P. striiformis f. sp. tritici pathotypes. Further testing of 600 F2 plants from a cross between WAWHT2046 and RIL143 (Yr48) with P. striiformis f. sp. tritici pathotype 134 E16A+Yr17+Yr27+ failed to reveal any susceptible segregants. Our results strongly suggest that Yr34 and Yr48 are the same gene, and that Yr48 should be considered a synonym of Yr34.

Molecular Mapping of a Novel QTL Conferring Adult Plant Resistance to Stripe Rust in Chinese Wheat Landrace ‘Guangtoumai’

Plant Disease ◽  
2020 ◽  
Author(s):  
Yu Wu ◽  
Yuqi Wang ◽  
Fangjie Yao ◽  
Li Long ◽  
Jing Li ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Plant Resistance ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Major Locus ◽  
Dcaps Marker ◽  
Chinese Wheat

Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat in the world. Chinese wheat landrace ‘Guangtoumai’ (GTM) exhibited a high-level resistance against predominant Pst races in China at the adult-plant stage. The objective of this research was to identify and map the major locus/loci for stripe rust resistance in GTM. A set of 212 recombinant inbred lines (RILs) was developed from a cross between GTM and Avocet S (AvS). The parents and RILs were evaluated in three field tests (2018, 2019, and 2020 at Chongzhou, Sichuan) with the currently predominant Pst races for final disease severity (FDS) and genotyped with the Wheat 55K SNP array to construct a genetic map with 1,031 SNP markers. A major locus, named QYr.GTM-5DL, was detected on chromosome 5DL in GTM. The locus was mapped in a 2.75 cM interval flanked by SNP markers AX-109855976 and AX-109453419, explaining up to 44.4% of the total phenotypic variation. Since no known Yr genes have been reported on chromosome 5DL, QYr.GTM-5DL is very likely a novel adult plant resistance (APR) locus. Haplotype analysis revealed that the resistance allele displayed enhanced levels of stripe rust resistance and is likely present in 5.3% of the 247 surveyed Chinese wheat landraces. The derived cleaved amplified polymorphic sequence (dCAPS) marker dCAPS-5722, converted from a SNP marker tightly linked to QYr.GTM-5DL with 0.3 cM, was validated on a subset of RILs and 48 commercial wheat cultivars developed in Sichuan. The results indicated that QYr.GTM-5DL with its linked dCAPS marker could be used in marker-assisted selection to improve stripe rust resistance in breeding programs, and this QTL will provide new and possibly durable resistance to stripe rust.

scholarly journals Genetic and Molecular Mapping of Stripe Rust Resistance Gene in Wheat–Psathyrostachys huashanica Translocation Line H9020-1-6-8-3

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1482-1487 ◽  
Author(s):  
Qiang Li ◽  
Jing Huang ◽  
Lu Hou ◽  
Pei Liu ◽  
Jinxue Jing ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Durable Resistance ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Translocation Line ◽  
Psathyrostachys Huashanica ◽  
Stripe Rust Resistance Gene

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. The best strategy to control stripe rust is to grow resistant cultivars, but only a few effective genes are available. The wheat accession H9020-1-6-8-3 is a translocation line previously developed from interspecific hybridization between wheat genotype 7182 and Psathyrostachys huashanica, and is resistant to most Chinese Puccinia striiformis f. sp. tritici races. To identify the resistance genes in the translocation line, H9020-1-6-8-3 was crossed with susceptible genotype Mingxian 169, and seedlings of parents and F1, F2, and F3 progenies were tested with prevalent Chinese P. striiformis f. sp. tritici races CYR32 and CYR33 under controlled greenhouse conditions. The genetic results indicated that two single dominant genes in H9020-1-6-8-3 confer resistance to CYR32 and CYR33, respectively. The gene for resistance to CYR33 was temporarily designated as YrH9020. Six simple-sequence repeat markers were used to map the resistance gene to the short arm of wheat chromosome 2D, using 329 F2 plants tested with CYR33 in the greenhouse. The genetic distances of the two closest flanking markers, Xgwm261 and Xgwm455, were 4.4 and 5.8 centimorgans, respectively. Disease assessments and polymorphic tests of the flanking markers among the Psathyrostachys huashanica line and wheat lines 7182, H9020-1-6-8-3, and Mingxian169 suggested that the resistance gene YrH9020 in H9020-1-6-8-3 was originally from P. huashanica. The exotic stripe rust resistance gene and linked molecular markers should be useful for pyramiding with other genes to develop wheat cultivars with high-level and durable resistance to stripe rust.

scholarly journals Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

2020 ◽  
Author(s):  
Yuqi Wang ◽  
Can Yu ◽  
Yukun Cheng ◽  
Fangjie Yao ◽  
Li Long ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Plant Resistance ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Landrace ◽  
Landrace Diversity ◽  
Chinese Wheat ◽  
Rust Resistance Genes

Abstract Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant varieties are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions, in addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under controlled greenhouse conditions.Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments under field tests, while four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers covering the whole genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, while four and one QTL conferring seedling and adult-plant resistance respectively were mapped distantly from previously reported stripe rust resistance genes or QTL and may be novel resistance loci.Conclusions: Our results provided an integrated view of stripe rust resistance resources in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.

scholarly journals Genome-wide association mapping reveals potential novel loci controlling stripe rust resistance in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone

2020 ◽  
Author(s):  
Yuqi Wang ◽  
Can Yu ◽  
Yukun Cheng ◽  
Fangjie Yao ◽  
Li Long ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Plant Resistance ◽  
Rust Resistance ◽  
Adult Plant Resistance ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Wheat Landrace ◽  
Landrace Diversity ◽  
Chinese Wheat ◽  
Rust Resistance Genes

Abstract Background: Stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious foliar disease of wheat. Identification of novel stripe rust resistance genes and cultivation of resistant varieties are considered to be the most effective approaches to control this disease. In this study, we evaluated the infection type (IT), disease severity (DS) and area under the disease progress curve (AUDPC) of 143 Chinese wheat landrace accessions for stripe rust resistance. Assessments were undertaken in five environments at the adult-plant stage with Pst mixture races under field conditions, in addition, IT was assessed at the seedling stage with two prevalent Pst races (CYR32 and CYR34) under controlled greenhouse conditions. Results: Seventeen accessions showed stable high-level resistance to stripe rust across all environments under field tests, while four accessions showed resistance to the Pst races CYR32 and CYR34 at the seedling stage. Combining phenotypic data from the field and greenhouse trials with 6404 markers covering the whole genome, we detected 17 quantitative trait loci (QTL) on 11 chromosomes for IT associated with seedling resistance and 15 QTL on seven chromosomes for IT, final disease severity (FDS) or AUDPC associated with adult-plant resistance. Four stable QTL detected on four chromosomes, which explained 9.99%–23.30% of the phenotypic variation, were simultaneously associated with seedling and adult-plant resistance. Integrating a linkage map of stripe rust resistance in wheat, 27 QTL overlapped with previously reported genes or QTL, while four and one QTL conferring seedling and adult-plant resistance respectively were mapped distantly from previously reported stripe rust resistance genes or QTL and may be novel resistance loci. Conclusions: Our results provided an integrated view of stripe rust resistance resources in a Chinese wheat landrace diversity panel from the southern autumn-sown spring wheat zone. The identified resistant accessions and resistance loci will be useful in the ongoing effort to develop new wheat cultivars with strong resistance to stripe rust.

scholarly journals Quantitative Trait Loci Mapping of Adult Plant and Seedling Resistance to Stripe Rust (Puccinia striiformis Westend.) in a Multiparent Advanced Generation Intercross Wheat Population

2021 ◽  
Vol 12 ◽  
Author(s):  
Sandra Rollar ◽  
Manuel Geyer ◽  
Lorenz Hartl ◽  
Volker Mohler ◽  
Frank Ordon ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Wheat Population ◽  
Advanced Generation

Stripe rust caused by the biotrophic fungus Puccinia striiformis Westend. is one of the most important diseases of wheat worldwide, causing high yield and quality losses. Growing resistant cultivars is the most efficient way to control stripe rust, both economically and ecologically. Known resistance genes are already present in numerous cultivars worldwide. However, their effectiveness is limited to certain races within a rust population and the emergence of stripe rust races being virulent against common resistance genes forces the demand for new sources of resistance. Multiparent advanced generation intercross (MAGIC) populations have proven to be a powerful tool to carry out genetic studies on economically important traits. In this study, interval mapping was performed to map quantitative trait loci (QTL) for stripe rust resistance in the Bavarian MAGIC wheat population, comprising 394 F6 : 8 recombinant inbred lines (RILs). Phenotypic evaluation of the RILs was carried out for adult plant resistance in field trials at three locations across three years and for seedling resistance in a growth chamber. In total, 21 QTL for stripe rust resistance corresponding to 13 distinct chromosomal regions were detected, of which two may represent putatively new QTL located on wheat chromosomes 3D and 7D.

scholarly journals Identification of Candidate Genes and Genomic Regions Associated with Adult Plant Resistance to Stripe Rust in Spring Wheat

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2585
Author(s):  
Amira M. I. Mourad ◽  
Mohamed A. Abou-Zeid ◽  
Shamseldeen Eltaher ◽  
P. Stephen Baenziger ◽  
Andreas Börner
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Yield ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
P Value ◽  
Dart Markers ◽  
Single Marker Analysis ◽  
Gene Models

Wheat stripe rust (caused by Puccinia striiformis f. sp. tritici) is a major disease that damages wheat plants and affects wheat yield all over the world. In recent years, stripe rust became a major problem that affects wheat yield in Egypt. New races appeared and caused breakdowns in the resistant genotypes. To improve resistance in the Egyptian genotypes, new sources of resistance are urgently needed. In the recent research, a set of 95 wheat genotypes collected from 19 countries, including Egypt, were evaluated for their resistance against the Egyptian race(s) of stripe rust under field conditions in the two growing seasons 2018/2019 and 2019/2020. A high genetic variation was found among the tested genotypes. Single marker analysis was conducted using a subset of 71 genotypes and 424 diversity array technology (DArT) markers, well distributed across the genome. Out of the tested markers, 13 stable markers were identified that were significantly associated with resistance in both years (p-value ≤ 0.05). By using the sequence of the DArT markers, the chromosomal position of the significant DArT markers was detected, and nearby gene models were identified. Two markers on chromosomes 5A and 5B were found to be located within gene models functionally annotated with disease resistance in plants. These two markers could be used in marker-assisted selection for stripe rust resistance under Egyptian conditions. Two German genotypes were carrying the targeted allele of all the significant DArT markers associated with stripe rust resistance and could be used to improve resistance under Egyptian conditions.

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