scholarly journals Molecular mapping as a tool for pre-emptive breeding for resistance to the exotic barley pathogen, Puccinia striiformis f. sp. hordei

2003 ◽  
Vol 54 (12) ◽  
pp. 1351 ◽  
Author(s):  
M. Cakir ◽  
M. Spackman ◽  
C. R. Wellings ◽  
N. W. Galwey ◽  
D. B. Moody ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Molecular Mapping ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Sources Of Resistance ◽  
Plant Stage ◽  
The Usa ◽  
Alternative Sources ◽  
Barley Stripe Rust

Barley stripe rust (BSR), caused by Puccinia striiformis f. sp. hordei, has been a major disease problem to the barley industry worldwide that has not, to date, been detected in Australia. This paper describes the mapping of stripe rust resistance genes in Tallon/Kaputar (TK) and Arapiles/Franklin (AF) populations. The paper also reports on the usefulness of markers associated with alternative sources of resistance previously identified in the varieties Orca and Shyri in the USA. Stripe rust screening was conducted at the adult plant stage in Toluca, Mexico, for 2 years. Two major quantitative trait loci (QTLs) were found on chromosomes 2H and 5H in both populations. One region on chromosome 5H was highly significantly associated with resistance to stripe rust (R2 = 68% and 34% in TK and AF, respectively). The QTL on chromosome 2H accounted for 36% of the variation in TK and 10% of the variation in the AF population. These associations were consistent over both years. Further work will involve screening for additional markers in the target regions to identify polymorphism that can be used to select for multiple resistances in the absence of the pathogen.

scholarly journals Molecular Mapping of Stripe Rust Resistance Gene Yr81 in a Common Wheat Landrace Aus27430

Plant Disease ◽  
2019 ◽  
Vol 103 (6) ◽  
pp. 1166-1171 ◽  
Author(s):  
Mesfin Gessese ◽  
Harbans Bariana ◽  
Debbie Wong ◽  
Matthew Hayden ◽  
Urmil Bansal
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Chromosomal Assignment ◽  
Resistance Locus ◽  
Sources Of Resistance ◽  
Stripe Rust Resistance Gene ◽  
Ril Population

The deployment of diverse sources of resistance in new cultivars underpins durable control of rust diseases. Aus27430 exhibited a moderate level of stripe rust resistance against Puccinia striiformis f. sp. tritici (Pst) pathotypes currently prevalent in Australia. Aus27430 was crossed with the susceptible parent Avocet S (AvS) and subsequent filial generations were raised. Monogenic segregation observed among Aus27430/AvS F3 families was confirmed through stripe rust screening of an F6 recombinant inbred line (RIL) population, and the resistance locus was temporarily named YrAW5. Selective genotyping using an Illumina iSelect 90K wheat SNP bead chip array located YrAW5 in chromosome 6A. Genetic mapping of the RIL population with linked 90K SNPs that were converted into PCR-based marker assays, as well as SSR markers previously mapped to chromosome 6A, confirmed the chromosomal assignment for YrAW5. Comparative analysis of other stripe rust resistance genes located in chromosome 6A led to the formal designation of YrAW5 as Yr81. Tests with a marker linked with Yr18 also demonstrated the presence of this gene in Aus27430. Yr18 interacted with Yr81 to produce stripe rust responses lower than those produced by RILs carrying these genes individually. Although gwm459 showed higher recombination with Yr81 compared with the other flanking marker KASP_3077, it amplified the AvS allele in 80 cultivars, whereas KASP_3077 amplified AvS allele in 67 cultivars. Both markers can be used in marker-assisted selection after confirming parental polymorphism.

scholarly journals Barley Stripe Rust Resistance QTL: Development and Validation of SNP Markers for Resistance to Puccinia striiformis f. sp. hordei

2016 ◽  
Vol 106 (11) ◽  
pp. 1344-1351 ◽  
Author(s):  
K. Esvelt Klos ◽  
T. Gordon ◽  
P. Bregitzer ◽  
P. Hayes ◽  
X. M. Chen ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Recombinant Inbred Lines ◽  
Snp Markers ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Resistance Qtl ◽  
Barley Stripe Rust

Quantitative trait loci (QTL) for barley stripe rust resistance were mapped in recombinant inbred lines (RIL) from a ‘Lenetah’ × ‘Grannelose Zweizeilige’ (GZ) cross. GZ is known for a major seedling resistance QTL on chromosome 4H but linked markers suitable for marker-assisted selection have not been developed. This study identified the 4H QTL (log of the likelihood [LOD] = 15.94 at 97.19 centimorgans [cM]), and additional QTL on chromosomes 4H and 6H (LOD = 5.39 at 72.7 cM and 4.24 at 34.46 cM, respectively). A QTL on chromosome 7H (LOD = 2.04 at 81.07 cM) was suggested. All resistance alleles were derived from GZ. Evaluations of adult plant response in Corvallis, OR in 2013 and 2015 provided evidence of QTL at the same positions. However, the minor QTL on 4H was not statistically significant in either location/year, while the 7H QTL was significant in both. The single-nucleotide polymorphism markers flanking the resistance QTL were validated in RIL from a ‘95SR316A’ × GZ cross for their ability to predict seedling resistance. In 95SR316A × GZ, 91 to 92% of RIL with GZ alleles at the major 4H QTL and at least one other were resistant to moderate in reaction. In these populations, at least two QTL were required to transfer the barley stripe rust resistance from GZ.

scholarly journals A Genome-Wide Association Study of Resistance to Puccinia striiformis f. sp. hordei and P. graminis f. sp. tritici in Barley and Development of Resistant Germplasm

2020 ◽  
Vol 110 (5) ◽  
pp. 1082-1092 ◽  
Author(s):  
Javier Hernandez ◽  
Alicia del Blanco ◽  
Tanya Filichkin ◽  
Scott Fisk ◽  
Lynn Gallagher ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Stem Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Seedling Stage ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Adult Plant Stage ◽  
A Genome ◽  
Plant Stage

Stripe rust (incited by Puccinia striiformis f. sp. hordei) and stem rust (incited by P. graminis f. sp. tritici) are two of the most important diseases affecting barley. Building on prior work involving the introgression of the resistance genes rpg4/Rpg5 into diverse genetic backgrounds and the discovery of additional quantitative trait locus (QTLs) for stem rust resistance, we generated an array of germplasm in which we mapped resistance to stripe rust and stem rust. Stem rust races TTKSK and QCCJB were used for resistance mapping at the seedling and adult plant stages, respectively. Resistance to stripe rust, at the adult plant stage, was determined by QTLs on chromosomes 1H, 4H, and 5H that were previously reported in the literature. The rpg4/Rpg5 complex was validated as a source of resistance to stem rust at the seedling stage. Some parental germplasm, selected as potentially resistant to stem rust or susceptible but having other positive attributes, showed resistance at the seedling stage, which appears to be allelic to rpg4/Rpg5. The rpg4/Rpg5 complex, and this new allele, were not sufficient for adult plant resistance to stem rust in one environment. A QTL on 5H, distinct from Rpg5 and a previously reported resistance QTL, was required for resistance at the adult plant stage in all environments. This QTL is coincident with the QTL for stripe rust resistance. Germplasm with mapped genes/QTLs conferring resistance to stripe and stem rust was identified and is available as a resource to the research and breeding communities.

scholarly journals Development and molecular characterization of wheat-Aegilops peregrina Introgression Lines with resistance to leaf rust and stripe rust

2017 ◽  
Author(s):  
Deepika Narang ◽  
Satinder Kaur ◽  
Jyoti Saini ◽  
Parveen Chhuneja
Keyword(s):  
Leaf Rust ◽  
Molecular Characterization ◽  
Stripe Rust ◽  
Puccinia Striiformis ◽  
Puccinia Triticina ◽  
Introgression Lines ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Plant Stage

AbstractA wild non-progenitor species from wheat tertiary gene pool Aegilops peregrina accession pau3519 (UUSS) was used for introgression of leaf rust and stripe rust resistance in bread wheat. It was crossed and backcrossed with hexaploid wheat line Chinese Spring PhI to develop two homozygous BC2F6 wheat-Ae. peregrina introgression lines (ILs) viz. IL pau16058 and IL pau16061 through induced homoeologous recombination. Homozygous lines were screened against six Puccinia triticina and two Puccinia striiformis f. sp. tritici pathotypes at the seedling stage and a mixture of prevalent pathotypes of both rust pathogens at the adult plant stage. IL pau16061 showed resistance to leaf rust only while IL pau16058 was resistant to both leaf and stripe rust pathotypes throughout plant life. Molecular characterization of these ILs aided in defining the introgressed regions. Identification of linked markers with advance genomic technologies will aid in marker assisted pyramiding of alien genes in cultivated wheat background.

Evaluation of Central Asian wheat germplasm for stripe rust resistance

2017 ◽  
Vol 16 (2) ◽  
pp. 178-184 ◽  
Author(s):  
Alma Kokhmetova ◽  
Ram C. Sharma ◽  
Shynbolat Rsaliyev ◽  
Kanat Galymbek ◽  
Kanagat Baymagambetova ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Breeding Lines ◽  
Central Asian ◽  
Plant Stage ◽  
Breeding Programmes ◽  
New Varieties

AbstractStripe rust, caused by Puccinia striiformis f.sp. tritici (Pst), is an important disease of winter wheat in Central Asia. Stripe rust races contain diverse virulence/avirulence patterns and change rapidly. Therefore the objectives of this research were to: (i) examine current pathotype variability of Pst races collected from Kazakhstan and Uzbekistan and (ii) evaluate stripe rust resistance in leading cultivars and advanced breeding lines targeted to those regions. Analyses of 152 Pst samples showed diverse virulence patterns with avirulence to Yr5, Yr10 and Yr15 being common. Most of identified races are among the rare. Analysis of a mixed Pst population showed 10 distinct pathotypes with frequencies ranged from 1.2 to 8.7%. The virulence patterns ranged from least ‘31–1.5’ and X-1.5 to highly virulent ‘86 + E16’. Seedling evaluation of 62 genotypes using the 10 pathotypes showed variations for resistance. Bunyodkor and Barhayot showed resistance to all pathotypes. Five Yr genes were postulated. Yr1 in KR12-5075, and Yr6 in KR11-03 and KR12-5003 were postulated. Yr5 combined with Yr10 and Yr15 genes were determined in Bunyodkor. The wheat genotypes also showed different levels of resistance in adult plant stage under field conditions. Twenty genotypes showed <20% severity in both Kazakhstan and Uzbekistan. The disease severity on several genotypes differed in this countries, suggesting different Pst populations in the two countries. Several resistant genotypes were identified, which should be further evaluated for release as new varieties or used in breeding programmes. Two resistant lines from this study were identified as new varieties in Georgia and Uzbekistan.

scholarly journals Molecular Mapping of YrSP and Its Relationship with Other Genes for Stripe Rust Resistance in Wheat Chromosome 2BL

2015 ◽  
Vol 105 (9) ◽  
pp. 1206-1213 ◽  
Author(s):  
J. Y. Feng ◽  
M. N. Wang ◽  
X. M. Chen ◽  
D. R. See ◽  
Y. L. Zheng ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Chromosomal Location ◽  
Molecular Mapping ◽  
Puccinia Striiformis ◽  
Dominant Gene ◽  
The United States ◽  
Stripe Rust Resistance ◽  
Recurrent Parent ◽  
Isogenic Line ◽  
Sts Markers

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important disease of wheat worldwide. Resistance is the best way to control the disease. YrSP, a gene originally from ‘Spaldings Prolific’ wheat and providing resistance to a broad spectrum of races, is used for differentiating P. striiformis f. sp. tritici races but its chromosomal location is not clear. To map YrSP, a near-isogenic line (AvSYrSPNIL) was backcrossed to the recurrent parent, Avocet S. Genetic analysis of the BC7F1, BC8, BC7F2, and BC7F3 progenies confirmed a single dominant gene for resistance. In total, 182 BC7F2 plants and their derived BC7F3 lines were phenotyped with an avirulent P. striiformis f. sp. tritici race and genotyped with simple-sequence repeat (SSR), single-nucleotide polymorphism (SNP), and sequence-tagged site (STS) markers. A linkage map was constructed with 3 SSR, 17 SNP, and 3 STS markers covering 23.3 centimorgans (cM). Markers IWA638 and dp269 were 0.6 cM proximal and 1.5 cM distal, respectively, to YrSP. The gene was mapped in chromosome bin 2BL-C-0.5, physically within the proximal 50% of the chromosome 2BL arm. Allelism tests based on F2 phenotypes indicated that YrSP is closely linked to but not allelic with genes Yr5, Yr7, Yr43, Yr44, and Yr53. Infection type data from tests with 10 historical and currently predominant P. striiformis f. sp. tritici races in the United States also demonstrated differences in specificity between YrSP and the other genes. The specificity of YrSP is useful in differentiating P. striiformis f. sp. tritici races and studying the plant–pathogen interactions, and the information of chromosomal location of the gene and its tightly linked markers should be useful in developing resistant cultivars when combined with other genes for resistance to stripe rust.

High-temperature adult-plant resistance to stripe rust in facultative winter wheat

2016 ◽  
Vol 67 (10) ◽  
pp. 1064 ◽  
Author(s):  
Beyhan Akin ◽  
Xian Ming Chen ◽  
Alex Morgunov ◽  
Nusret Zencirci ◽  
Anmin Wan ◽  
...  
Keyword(s):  
Molecular Markers ◽  
High Temperature ◽  
Winter Wheat ◽  
Stripe Rust ◽  
Field Experiments ◽  
Puccinia Striiformis ◽  
Yellow Rust ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Improvement Program

Stripe (yellow) rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss., is one of the most damaging diseases in wheat and is especially damaging for winter and facultative wheat. The objective of this study was to understand stripe rust resistance in 100 wheat and facultative wheat entries from the International Winter Wheat Improvement Program by conducting experiments in a greenhouse and in four field environments in Washington State, USA, and by genotyping molecular markers linked to Yr genes. Percentages of entries resistant to the rust races at the seedling stage were: PST-17, 44%; PST-37, 32%; PST-43, 45%; PST-45, 49%; PST-116, 18%; PST-100, 17%; and PST-127, 8%. Molecular markers were positive for genes Yr9, Yr17, and Yr18 and negative for Yr5, Yr10, and Yr15. Yr18 was present in 44 entries (44%). By using the highly virulent races PST-127 and PST-100 under controlled conditions, 16 entries were shown to have high-temperature adult-plant (HTAP) resistance and resistant–moderately resistant field reactions at all four field sites. Resistant entries, especially those with HTAP resistance, were also identified in the field experiments.

scholarly journals Quantitative Trait Loci Mapping of Adult Plant and Seedling Resistance to Stripe Rust (Puccinia striiformis Westend.) in a Multiparent Advanced Generation Intercross Wheat Population

2021 ◽  
Vol 12 ◽  
Author(s):  
Sandra Rollar ◽  
Manuel Geyer ◽  
Lorenz Hartl ◽  
Volker Mohler ◽  
Frank Ordon ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Wheat Population ◽  
Advanced Generation

Stripe rust caused by the biotrophic fungus Puccinia striiformis Westend. is one of the most important diseases of wheat worldwide, causing high yield and quality losses. Growing resistant cultivars is the most efficient way to control stripe rust, both economically and ecologically. Known resistance genes are already present in numerous cultivars worldwide. However, their effectiveness is limited to certain races within a rust population and the emergence of stripe rust races being virulent against common resistance genes forces the demand for new sources of resistance. Multiparent advanced generation intercross (MAGIC) populations have proven to be a powerful tool to carry out genetic studies on economically important traits. In this study, interval mapping was performed to map quantitative trait loci (QTL) for stripe rust resistance in the Bavarian MAGIC wheat population, comprising 394 F6 : 8 recombinant inbred lines (RILs). Phenotypic evaluation of the RILs was carried out for adult plant resistance in field trials at three locations across three years and for seedling resistance in a growth chamber. In total, 21 QTL for stripe rust resistance corresponding to 13 distinct chromosomal regions were detected, of which two may represent putatively new QTL located on wheat chromosomes 3D and 7D.

scholarly journals Defining the genetic architecture of stripe rust resistance in the barley accession HOR 1428

2016 ◽  
Author(s):  
Shaun Clare ◽  
William Kitcher ◽  
Matthew Gardiner ◽  
Phon Green ◽  
Amelia Hubbard ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Genetic Architecture ◽  
Genetic Resource ◽  
Puccinia Striiformis ◽  
Stripe Rust Resistance ◽  
Seedling Resistance ◽  
Major Constraint ◽  
Barley Stripe Rust ◽  
Kasp Marker ◽  
Mapping Information

ABSTRACTPuccinia striiformis f. sp. hordei, the causal agent of barley stripe rust, is a destructive fungal pathogen that significantly affects barley cultivation. A major constraint in breeding resistant cultivars is the lack of mapping information of resistance (R) genes and their introgression into adapted germplasm. A considerable number of R genes have been described in barley to P. striiformis f. sp. hordei, but only a few loci have been mapped. Previously, Chen and Line (1999) reported two recessive seedling resistance loci in the Ethiopian landrace HOR 1428. In this study, we map two loci that confer resistance to P. striiformis f. sp. hordei in HOR 1428, which are located on chromosomes 3H and 5H. Both loci act as additive effect QTLs, each explaining approximately 20% of the phenotypic variation. We backcrossed HOR 1428 to the cv. Manchuria and selected based on markers flanking the RpsHOR128-5H locus. Saturation of the RpsHOR1428-5H locus with markers in the region found KASP marker K_1_0292 in complete coupling with resistance to P. striiformis f. sp. hordei and was designated Rps9. Isolation of Rps9 and flanking markers will facilitate the deployment of this genetic resource into existing programs for P. striiformis f. sp. hordei resistance.

scholarly journals Identification of Candidate Genes and Genomic Regions Associated with Adult Plant Resistance to Stripe Rust in Spring Wheat

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2585
Author(s):  
Amira M. I. Mourad ◽  
Mohamed A. Abou-Zeid ◽  
Shamseldeen Eltaher ◽  
P. Stephen Baenziger ◽  
Andreas Börner
Keyword(s):  
Stripe Rust ◽  
Rust Resistance ◽  
Puccinia Striiformis ◽  
Wheat Yield ◽  
Stripe Rust Resistance ◽  
Adult Plant ◽  
P Value ◽  
Dart Markers ◽  
Single Marker Analysis ◽  
Gene Models

Wheat stripe rust (caused by Puccinia striiformis f. sp. tritici) is a major disease that damages wheat plants and affects wheat yield all over the world. In recent years, stripe rust became a major problem that affects wheat yield in Egypt. New races appeared and caused breakdowns in the resistant genotypes. To improve resistance in the Egyptian genotypes, new sources of resistance are urgently needed. In the recent research, a set of 95 wheat genotypes collected from 19 countries, including Egypt, were evaluated for their resistance against the Egyptian race(s) of stripe rust under field conditions in the two growing seasons 2018/2019 and 2019/2020. A high genetic variation was found among the tested genotypes. Single marker analysis was conducted using a subset of 71 genotypes and 424 diversity array technology (DArT) markers, well distributed across the genome. Out of the tested markers, 13 stable markers were identified that were significantly associated with resistance in both years (p-value ≤ 0.05). By using the sequence of the DArT markers, the chromosomal position of the significant DArT markers was detected, and nearby gene models were identified. Two markers on chromosomes 5A and 5B were found to be located within gene models functionally annotated with disease resistance in plants. These two markers could be used in marker-assisted selection for stripe rust resistance under Egyptian conditions. Two German genotypes were carrying the targeted allele of all the significant DArT markers associated with stripe rust resistance and could be used to improve resistance under Egyptian conditions.

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