Screening and selection of faba bean (Vicia faba L.) germplasm resistant to Bean leafroll virus

2002 ◽  
Vol 53 (9) ◽  
pp. 1077 ◽  
Author(s):  
Khaled M. Makkouk ◽  
Safaa G. Kumari ◽  
Joop A. G. van Leur

Three hundred and fifty-eight faba bean accessions, originating from 28 countries in 5 continents, were evaluated for their reaction to a Syrian isolate (SV64-95) of Bean leafroll virus (BLRV, family Luteoviridae). Selection for resistance was based on: (i) visual evaluation for symptom expression (disease severity); (ii) virus concentration in the plant tissues, based on the intensity of reaction in the tissue-blot immunoassay; and (iii) seed yield. A large variability in resistance to BLRV was found both between and within faba bean accessions. Progenies of single plants, selected for resistance under inbreeding conditions, were evaluated for another 2-year period. Repeated reselection of inoculated plants, in the absence of outcrossing, significantly increased the proportion of resistant plants. The most resistant selections were made from populations originating from Afghanistan, China, Colombia, Pakistan, Russia, Sudan, Switzerland, Tunisia, Turkey, and Yemen.

Plant Disease ◽  
2000 ◽  
Vol 84 (9) ◽  
pp. 1046-1046 ◽  
Author(s):  
Asma Najar ◽  
Khaled M. Makkouk ◽  
Safaa G. Kumari

A survey was conducted in April 2000 to identify viruses infecting faba bean (Vicia faba L.) in seven regions (Beja, Bizerte, Cap-bon, Jendouba, Kairouan, Siliana, and Zaghouan) of Tunisia. Around 4,000 random samples were collected from 34 fields (100 to 200 samples per field). Samples were tested by tissue-blot immunoassay procedure (2) at the Virology Laboratory of INRAT for the presence of 14 different viruses. All antisera used were specific, including those for Faba bean necrotic yellows virus (FBNYV) (1) and Beet western yellows virus (BWYV) (ATCC-PVAS-647). Broad bean mottle, a beetle-transmitted and seedborne virus, was the most abundant with an average incidence of 2.3%. The highest disease incidence occurred with BWYV, which was in 20 and FBNYV in 4 of 98 random samples from a field in the Siliana region; and with BWYV in 7 and BBMV in 21 of 120 samples from a field in the Jendouba region. Other viruses such as broad bean stain, cucumber mosaic, and bean leaf roll were detected rarely (<1%). This is the first record of FBNYV and BWYV infecting faba bean in Tunisia. BWYV was detected in 1.2% of the samples tested collected from 20 fields comprising all seven regions. FBNYV was detected in 0.9% of the samples tested collected from 13 fields comprising all except the Capbon region. References: (1) A. Franz et al. Ann. Appl. Biol. 128:255, 1996. (2) K. M. Makkouk and A. Comeau. Eur. J. Plant Pathol. 100:71, 1994.


Author(s):  
Narendra Kumar Dewangan ◽  
G.S. Dahiya ◽  
D.K. Janghel ◽  
Seema Dohare

Background: Faba bean (Vicia faba L.) is one of the oldest cool season food legume crops, stands next to soybean (Glycine max L.) and pea (Pisum sativum L.). A throughout knowledge of existing genetic variation among seed yield and its component traits is essential for developing high yielding varieties in faba bean. Realizing the importance of genetic diversity in key economic traits, the present investigation planned to assess the genetic diversity in faba bean germplasm for yield improvement in faba bean breeding programme. Methods: The experimental material comprised of 80 faba bean germplasm lines, grown in RBD with three replications at Research Farm of Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar during Rabi 2015-16. The data on 10 quantitative traits was analysed for Mahalanobis D2 statistic, stepwise multiple regression and principle component analysis (PCA).Result: The present study has assessed the existed genetic variations traits among faba bean germplasm lines for seed yield and its component traits. This would certainly provide guidelines in the selection of parents as well as effective selection of promising faba bean genotypes in faba bean breeding programmes for developing high yielding varieties.


2006 ◽  
Vol 114 (1) ◽  
pp. 59-66 ◽  
Author(s):  
N. Gutierrez ◽  
C. M. Avila ◽  
G. Duc ◽  
P. Marget ◽  
M. J. Suso ◽  
...  

2020 ◽  
Author(s):  
Moez Amri ◽  
Zouhaier Abbes ◽  
Imen Trabelsi ◽  
Michel Edmond Ghanem ◽  
Rachid Mentag ◽  
...  

AbstractIn total, 39 faba bean (Vicia faba L.) advanced lines were evaluated for resistance to broomrape Orobanche foetida under highly infested field conditions. The trials were conducted during two consecutive copping seasons at Oued-Beja Research Station in Tunisia. The advanced lines XAR-VF00.13-1-2-1-2-1 and XBJ90.04-2-3-1-1-1-2A expressed high resistance level to O. foetida exceeding those recorded for resistance checks Najeh and Baraca. Results showed that O. foetida significantly affected the biomass, grain yield, chlorophyll content index (CCI) and the maximum quantum efficiency (Fv/Fm ratio). No significant effect of O. foetida parasitism was observed on host plant water content (WC). O. foetida parasitism significantly affected both CCI and Fv/Fm ratio. CCI decreases varied from 46.4% for the susceptible check Badi and 4.2% and 9.3% observed for the genotypes Baraca and XBJ90.04-2-3-1-1-1-2A. Compared to susceptible check, slight decreases of Fv/Fm ratio were observed for both advanced lines XBJ90.04-2-3-1-1-1-2A and XAR-VF00.13-1-2-1-2-1. Correlation between CCI and Fv/Fm with the resistance to broomrape makes this, easy-to-measure, parameter very useful as a practical screening tool for early parasitism detection, diagnosis and identification and selection of high resistant plants against this pathogen.


Methodology ◽  
2018 ◽  
Vol 14 (4) ◽  
pp. 177-188 ◽  
Author(s):  
Martin Schultze ◽  
Michael Eid

Abstract. In the construction of scales intended for the use in cross-cultural studies, the selection of items needs to be guided not only by traditional criteria of item quality, but has to take information about the measurement invariance of the scale into account. We present an approach to automated item selection which depicts the process as a combinatorial optimization problem and aims at finding a scale which fulfils predefined target criteria – such as measurement invariance across cultures. The search for an optimal solution is performed using an adaptation of the [Formula: see text] Ant System algorithm. The approach is illustrated using an application to item selection for a personality scale assuming measurement invariance across multiple countries.


2016 ◽  
Vol 11 (3) ◽  
pp. 217
Author(s):  
Estu Nugroho ◽  
Budi Setyono ◽  
Mochammad Su’eb ◽  
Tri Heru Prihadi

Program pemuliaan ikan mas varietas Punten dilakukan dengan seleksi individu terhadap karakter bobot ikan. Pembentukan populasi dasar untuk kegiatan seleksi dilakukan dengan memijahkan secara massal induk ikan mas yang terdiri atas 20 induk betina dan 21 induk jantan yang dikoleksi dari daerah Punten, Kepanjen (delapan betina dan enam jantan), Kediri (tujuh betina dan 12 jantan), Sragen (27 betina dan 10 jantan), dan Blitar (15 betina dan 11 jantan). Larva umur 10 hari dipelihara selama empat bulan. Selanjutnya dilakukan penjarangan sebesar 50% dan benih dipelihara selama 14 bulan untuk dilakukan seleksi dengan panduan hasil sampling 250 ekor individu setiap populasi. Seleksi terhadap calon induk dilakukan saat umur 18 bulan pada populasi jantan dan betina secara terpisah dengan memilih berdasarkan 10% bobot ikan yang terbaik. Calon induk yang terseleksi kemudian dipelihara hingga matang gonad, kemudian dipilih sebanyak 150 pasang dan dipijahkan secara massal. Didapatkan respons positif dari hasil seleksi berdasarkan bobot ikan, yaitu 49,89 g atau 3,66% (populasi ikan jantan) dan 168,47 g atau 11,43% (populasi ikan betina). Nilai heritabilitas untuk bobot ikan adalah 0,238 (jantan) dan 0,505 (betina).Punten carp breeding programs were carried out by individual selection for body weight trait. The base population for selection activities were conducted by mass breeding of parent consisted of 20 female and 21 male collected from area Punten, eight female and six male (Kepanjen), seven female and 12 male (Kediri), 27 female and 10 male (Sragen), 15 female and 11 male (Blitar). Larvae 10 days old reared for four moths. Then after spacing out 50% of total harvest, the offspring reared for 14 months for selection activity based on the sampling of 250 individual each population. Selection of broodstock candidates performed since 18 months age on male and female populations separately by selecting based on 10% of fish with best body weight. Candidates selected broodstocks were then maintained until mature. In oder to produce the next generation 150 pairs were sets and held for mass spawning. The results revealed that selection response were positive, 49.89 g (3.66%) for male and 168.47 (11.43%) for female. Heritability for body weight is 0.238 (male) and 0.505 (female).


Confectionery sunflower - a special area of use of sunflower, which requires the creation of marketable seeds quality features. One of the possible ways to create large-fruited sunflower is to create production hybrids and lines. Objective: to evaluate the created new large-fruited sunflower lines by a complex of morphological characters and determine the best lines for use as large-seeds hybrids as parent components or source material. In 2016-2019 years on the basis of the Institute of Oilseed Crops NAAS a study was conducted to assess the economic characteristics of large-fruited sunflower lines. We studied a collection of 27 lines of large-seeds sources. The lines were created by direct selection or crossing and sampling: Reyny of Argentinean origin, Zaporizhzhya confectionery variety, confectionery hybrid with striped pericarp color of Israeli origin, white seed of Turkish origin, synthetic population - donor of complex resistance. To study from the collection, lines were drawn that went through at least 7 generations with selection for seed size. Experience has shown that the shortest growing season for lines 174d and KP11 was 99 days, and the longest for lines I2K670 was 109 days. In the studied collection, the greatest mass of 1000 seeds has the KP11-146.47g line, which is the mother component and does not have branching. The second by weight of 1000 seeds (109 g) stood out line 168v, which also had branches and pollen fertility restoration genes and will be used as the paternal form. The third largest is also one basket line ZKN51-100. The collection included lines originating from the same combination, but with a different morphotype for the presence and absence of branching. So, based on the combination of KP11 x Zaporizhzhya Confectionery, three lines were obtained. A mass of 1000 seeds was observed in 98-86 g, with the branching line having the largest mass of 1000 seeds. The lines created with one combination VK678 x ZKN32: with a branch 168a had a mass of 1000 seeds 95g, and a line 168b - without a branch 109 g. Of the two lines obtained from the descendants of the combination KP11 x the striped hybrid both had branches, but the seeds were much smaller (weight of 1000 seeds 59 and 79 g). The collection also studied samples created on the basis of varieties and populations 160c, 174, 175b, the mass of 1000 seeds of which turned out to be more acceptable for large-fruited use from 83 to 99 g. Summing up the results of studying the collection of newly created lines, we can highlight the lines 162d, 168v, 175b, KP11 that are potentially promising for use in hybrids. The selections showed that large-fruited lines can be obtained from large-fruited varieties, self-pollination of large-fruited hybrids and crossing lines with hybrids and varieties. Self-pollination and selection of large-fruited lines in several generations does not provide the necessary variability for positive changes in selections. The result of the selection by weight of 1000 seeds in the offspring from crosses and from populations creates opportunities for new large-seeds sunflower.


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