Location of lettuce mosaic virus in mature lettuce seed tissues by immunogold cytochemistry.

1991 ◽  
Vol 20 (1) ◽  
pp. 3 ◽  
Author(s):  
DG Hunter ◽  
JW Bowyer
Keyword(s):  
Mosaic Virus ◽  
Lettuce Seed ◽  
Lettuce Mosaic Virus ◽  
Immunogold Cytochemistry

Incidence of Lettuce Mosaic Virus in Imported Lettuce Seed

1970 ◽  
Vol 19 (4) ◽  
pp. 194-195 ◽  
Author(s):  
Christine M. Hall ◽  
A. H. Bennett
Keyword(s):  
Mosaic Virus ◽  
Lettuce Seed ◽  
Lettuce Mosaic Virus

scholarly journals Avaliação da reação de genótipos de alface (Lactuca sativa L.) ao Lettuce mosaic virus (LMV)

Bragantia ◽  
2007 ◽  
Vol 66 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Rosa Maria Chung ◽  
Joaquim Adelino de Azevedo Filho ◽  
Addolorata Colariccio
Keyword(s):  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Lactuca Sativa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lettuce Mosaic Virus ◽  
Lactuca Sativa L

O trabalho teve como meta avaliar a reação de 18 linhagens superiores do programa de melhoramento de alface (Lactuca sativa L.) do IAC e de seis cultivares comerciais, ao Lettuce mosaic virus (LMV). Em condições de campo, na região de Atibaia (SP), foram observados sintomas de mosaico, nanismo e necrose em plantas das cultivares Rider, 'Karla H25' e Hortência. O vírus presente nos isolados foi identificado por meio de inoculação mecânica em plantas indicadoras e diferenciadoras e de testes sorológicos de Plate Trapped Antigen-Enzyme linked-immunosorbent assay (PTA-ELISA). Nas amostras avaliadas, identificou-se a espécie LMV pelo PTA-ELISA e do patotipo IV pela reação nas hospedeiras diferenciais. Para a avaliação do comportamento dos genótipos de alface, foi empregado o LMV isolado 'Karla H25'. Foram submetidos à inoculação 24 genótipos de alface empregando-se, como controle positivo, a alface 'White Boston' por sua suscetibilidade ao LMV. O delineamento experimental foi inteiramente ao acaso e analisado pelo teste do qui-quadrado. Detectaram-se genótipos com comportamento de suscetibilidade e de tolerância. Nos genótipos 3 e 4, foram observadas plantas com comportamento de tolerância ao LMV isolado 'Karla H25', enquanto nos demais genótipos, constataram-se plantas com comportamento suscetível. O plantio de cultivares tolerantes pode ser uma alternativa aos prejuízos causados pela infecção pelo LMV com conseqüente diminuição do uso de produtos químicos para o controle dos afídeos vetores.

Nucleotide sequence of the 3′ terminal region of the genome of four Lettuce mosaic virus isolates from Greece and Yemen

1999 ◽  
Vol 144 (8) ◽  
pp. 1619-1626 ◽  
Author(s):  
F. Revers ◽  
R. A. A. van der Vlugt ◽  
S. Souche ◽  
M. Lanneau ◽  
H. Lot ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Terminal Region ◽  
Lettuce Mosaic Virus ◽  
Virus Isolates

The Effect of Lettuce Mosaic Virus on Plant Cells

1977 ◽  
Vol 90 (1) ◽  
pp. 27-30 ◽  
Author(s):  
Ana Šaric ◽  
Mercedes Wrischer
Keyword(s):  
Mosaic Virus ◽  
Plant Cells ◽  
Lettuce Mosaic Virus

scholarly journals Reaction of lettuce genotypes to Lettuce mosaic virus-Most (LMV-Most) and characterization of the translation factor eIF4E

2018 ◽  
Vol 53 (1) ◽  
pp. 125-129
Author(s):  
Mônika Fecury Moura ◽  
Norberto da Silva ◽  
Maria Isabel Motta Hoffmann ◽  
Marcelo Agenor Pavan ◽  
Renate Krause-Sakate
Keyword(s):  
Mosaic Virus ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Coding Region ◽  
Rt Pcr ◽  
Lettuce Mosaic Virus ◽  
Eukaryotic Translation ◽  
Translation Factor ◽  
Eukaryotic Translation Initiation

Abstract: The objective of this work was to evaluate lettuce genotypes for their reaction to Lettuce mosaic virus (LMV; Most-type, isolate AF-199) and variations of the eukaryotic translation initiation factor eIF4E. All inoculated genotypes were susceptible to LMV, which was detected by RT-PCR using specific primer pairs. However, the accessions 169501, 169501C, 172918A, and 162499 showed late development of symptoms that appeared only on the inoculated leaves. Sequencing of the coding region of eIF4E showed that these genotypes have an eIF4E0 (mol 0 ) standard typical for their susceptibility to LMV, indicating that the phenotype found is not correlated to nucleotide variations in this translation factor.

scholarly journals The Use of Green Fluorescent Protein-Tagged Recombinant Viruses to Test Lettuce mosaic virus Resistance in Lettuce

2002 ◽  
Vol 92 (2) ◽  
pp. 169-176 ◽  
Author(s):  
T. Candresse ◽  
O. Le Gall ◽  
B. Maisonneuve ◽  
S. German-Retana ◽  
E. Redondo
Keyword(s):  
Green Fluorescent Protein ◽  
Mosaic Virus ◽  
Fluorescent Protein ◽  
Lettuce Mosaic Virus ◽  
Recombinant Viruses ◽  
Systemic Movement ◽  
Resistance Breaking ◽  
Recessive Genes ◽  
Lettuce Breeding ◽  
Green Fluorescent

Seed certification and the use of cultivars containing one of two, probably allelic, recessive genes, mo11 and mo12, are the principal control methods for Lettuce mosaic virus (LMV) in lettuce. Although for a few LMV isolates, mo12 confers resistance with most isolates, the genes mo11 or mo12 confer a tolerance, and virus accumulation is readily detected in mo1-carrying plants. This phenotype complicates evaluation of the resistance status, in particular for mo11, for which there are no viral strains against which a true resistance is expressed. Two green fluorescent protein (GFP)-tagged viruses were constructed, derived from a non-resistance breaking isolate (LMV-0) and from a resistance-breaking isolate (LMV-E). An evaluation of 101 cultivars of known status was carried out with these recombinant viruses. Using the LMV-0-derived recombinant, identification of mo1-carrying cultivars was simple because, contrary to its wild-type parent, systemic movement of LMV-0-GFP was abolished in resistant plants. This assay detected four cases of misidentification of resistance status. In all these cases, further tests confirmed that the prior resistance status information was incorrect, so that a 100% correlation was observed between LMV-0-GFP behavior and the mo1 resistance status. Similarly, the LMV-E-derived recombinant allowed the identification of mo12 lettuce lines because its systemic movement was restricted in mo12 lines but not in susceptible or in mo11 lines. The tagged viruses were able to systemically invade another host, pea, irrespective of its resistance status against another member of the genus Potyvirus, Pea seed-borne mosaic virus. The use of these recombinant viruses could therefore greatly facilitate LMV resistance evaluation and speed up lettuce breeding programs.

Identification of PCR-based markers flanking the recessive LMV resistance gene mo1 in an intraspecific cross in lettuce

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 982-986 ◽  
Author(s):  
S V Irwin ◽  
R V Kesseli ◽  
W Waycott ◽  
E J Ryder ◽  
J J Cho ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Disease Resistance Gene ◽  
Symptom Development ◽  
Recessive Resistance ◽  
Lettuce Mosaic Virus ◽  
Recessive Resistance Gene ◽  
The Common

Molecular markers flanking the recessive resistance gene mo1 were identified following analysis of two segregating populations. Generation of a population from a crisphead × crisphead cross of Lactuca sativa that segregated for resistance conferred by the mo12 allele, but not morphological traits, was required for accurate mapping of the gene. Resistance was best assessed by symptom development after inoculating F3 families with the common pathotype II isolate of lettuce mosaic virus (LMV). Bulked segregant analysis for RAPD (random amplified polymorphic DNA) markers using one population, followed by linkage analysis using another, identified markers on either side of the mo1 gene, approximately 8 cM apart. These markers will be useful for more efficient introgression of this resistance gene into additional lettuce cultivars.Key words: lettuce, lettuce mosaic virus, molecular marker, disease resistance gene, bulked segregant analysis.

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