Isolates of Fusarium oxysporum f. sp. cubense from wilted banana plants in Queensland and New South Wales were characterised for vegetative compatibility. Six vegetative compatibility groups VCGs) were identified. Race 1 (VCGs 0124, 01241.5, 0125) was widespread, being detected in northern and southern Queensland as well as northern New South Wales. Race 2 (VCG 0128) was found attacking Bluggoe in North Queensland. Race 4 (VCGs 0120, 0129, 01211) was detected in Cavendish plantations in southern Queensland and in Lady finger plantations in New (South Wales and southern Queensland. Isolates of the race 4 VCG 0129 from Lady finger plantations were pathogenic to Cavendish cultivars in glasshouse tests.