Involvement of silicon in pearl millet resistance to downy mildew disease and its interplay with cell wall proline/hydroxyproline-rich glycoproteins

2008 ◽  
Vol 37 (5) ◽  
pp. 498 ◽  
Author(s):  
S. Deepak ◽  
G. Manjunath ◽  
S. Manjula ◽  
S. Niranjan-Raj ◽  
N. P. Geetha ◽  
...  
Keyword(s):  
Cell Wall ◽  
Downy Mildew ◽  
Pearl Millet ◽  
Downy Mildew Disease

scholarly journals Systemic protection against pearl millet downy mildew disease induced by cell wall glucan elicitors from Trichoderma hamatum UOM 13

2017 ◽  
Vol 57 (3) ◽  
pp. 296-308 ◽  
Author(s):  
Senapathyhally Nagaraju Lavanya ◽  
Sathyanarayana Niranjan-Raj ◽  
Siddaiah Chandra Nayaka ◽  
Kestur Nagaraju Amruthesh
Keyword(s):  
Cell Wall ◽  
Downy Mildew ◽  
Pearl Millet ◽  
Seed Treatment ◽  
Foliar Spray ◽  
Delivery Methods ◽  
Defense Proteins ◽  
Trichoderma Hamatum ◽  
Mycelial Extract ◽  
Downy Mildew Disease

Abstract The obligate oomycete Sclerospora graminicola (Sacc.) Schroet, is the incitant of downy mildew disease, which is the main constraint in pearl millet production worldwide. Different elicitors from Trichoderma hamatum UOM 13, e.g. mycelial extract and cell wall glucans, were assessed for their resistance elicitation efficiency and the possible underlying mechanisms. Both mycelial extract and cell wall glucans of T. hamatum UOM 13 positively influenced seed quality parameters of pearl millet, significantly enhanced seed germination and seedling vigor in comparison to the untreated control. Seed priming with cell wall glucan elicitors of T. hamatum UOM 13 suppressed downy mildew on susceptible pearl millet seedlings under greenhouse conditions by induction of systemic host resistance. Of the different elicitor delivery methods tested, transplant root dip was more effective than seed treatment and foliar spray. A combination of transplant root dip + seed treatment + foliar spray was significantly more effective than the single delivery methods. The induced resistance corresponded to up regulation of genes of important defense proteins upon pathogen inoculation. Transcripts of genes of defense enzymes glucanase, phenylalanine ammonia lyase, peroxidase and polyphenoloxidase were significantly increased due to the T. hamatum UOM elicitor effect. Expression of hydroxyproline-rich glycoprotein genes, known to play an important role in cell wall cross-linking, were also up regulated in response to T. hamatum UOM cell wall glucan treatment. This study emphasizes the role of T. hamatum UOM as a potential elicitor of downy mildew resistance in pearl millet and presents novel insights into the involvement of important defense proteins mediating such as resistance trigger.

Spore cell wall components ofAspergillus niger elicit downy mildew disease resistance in pearl millet

2006 ◽  
Vol 34 (1) ◽  
pp. 72-86 ◽  
Author(s):  
C. K. Hindumathy ◽  
S. Shailasree ◽  
K. Ramachandra Kini ◽  
H. Shekar Shetty
Keyword(s):  
Cell Wall ◽  
Disease Resistance ◽  
Downy Mildew ◽  
Pearl Millet ◽  
Cell Wall Components ◽  
Wall Components ◽  
Spore Cell ◽  
Downy Mildew Disease

scholarly journals Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

2016 ◽  
Vol 7 ◽  
Author(s):  
Sharathchandra R. Govind ◽  
Sudisha Jogaiah ◽  
Mostafa Abdelrahman ◽  
Hunthrike S. Shetty ◽  
Lam-Son P. Tran
Keyword(s):  
Downy Mildew ◽  
Pearl Millet ◽  
Downy Mildew Disease

scholarly journals Isolation and evaluation of proteolytic actinomycete isolates as novel inducers of pearl millet downy mildew disease protection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Sudisha Jogaiah ◽  
Mahantesh Kurjogi ◽  
Sharathchandra Ramasandra Govind ◽  
Shekar Shetty Huntrike ◽  
Vedamurthy Ankala Basappa ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Pearl Millet ◽  
Downy Mildew Disease

Resistance to downy mildew in pearl millet is associated with increased phenylalanine ammonia lyase activity

2005 ◽  
Vol 32 (3) ◽  
pp. 267 ◽  
Author(s):  
N. P. Geetha ◽  
K. N. Amruthesh ◽  
R. G. Sharathchandra ◽  
H. S. Shetty
Keyword(s):  
Enzyme Activity ◽  
Downy Mildew ◽  
Pearl Millet ◽  
Phenylalanine Ammonia Lyase ◽  
Cultured Cells ◽  
Enzyme Activation ◽  
Sclerospora Graminicola ◽  
Pathogen Infection ◽  
Pal Activity ◽  
Downy Mildew Disease

Phenylalanine ammonia lyase (PAL) activity was studied in pearl millet cultivars with different levels of resistance to the downy mildew disease caused by Sclerospora graminicola, an important oomycete pathogen. PAL activity was elevated in resistant host cultivar and decreased in susceptible cultivars following downy mildew pathogen infection. The enzyme activation varied between cultivars and was correlated with the degree of resistance to downy mildew disease. The induction of PAL as a response to pathogen inoculation was further corroborated by a time-course study in seedlings and cultured cells of pearl millet. The level of PAL activity was highest at 1.5 h in cultured cells and 4 h in seedlings of resistant host cultivar after inoculation with Sclerospora graminicola. Further studies on PAL activity in different tissues of seedlings showed highest enzyme activity in the young growing region of the root of the resistant host cultivars. The accumulation of wall-bound phenolics and lignin was higher in the resistant cultivar seedlings as evidenced by phloroglucinol–HCl staining and p-coumaric acid assay. The temporal changes in lignin concentration and the concentration of soluble phenolics were greater in root tissues of resistant cultivars than in those of susceptible cultivars. Treatment of resistant seedlings with a PAL inhibitor, α-aminooxy-β-phenylpropionic acid, resulted in the enhancement of the enzyme activity, whereas in the presence of 1 mm trans-cinnamic acid the pathogen-induced PAL was completely inhibited. Treatment of pearl millet seedlings with exogenously applied PAL inhibitors induced downy mildew disease susceptibility in the resistant pearl millet cultivar, consistent with direct involvement of PAL in downy mildew resistance. Results are discussed with respect to the presumed importance of host phenolic compounds and lignin accumulation and its relation to PAL activation as a response to the pathogen infection.

Antisporulant activity of leaf extracts of Indian plants againstSclerospora graminicolacausing downy mildew disease of pearl millet

2005 ◽  
Vol 38 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Saligrama Adavigowda Deepak ◽  
Gyula Oros ◽  
Syagadadu Giriyanna Sathyanarayana ◽  
Nandini Pratap Shetty ◽  
Huntrike Shekar Shetty ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Pearl Millet ◽  
Leaf Extracts ◽  
Downy Mildew Disease
Sign in / Sign up

Export Citation Format

Share Document