Discovery of single nucleotide polymorphisms in bone morphogenetic protein (BMP) genes of goats by double digest restriction-site associated DNA sequencing

2021 ◽  
Author(s):  
Shalu Elizabeth Simon ◽  
G. Radhika ◽  
T. V. Aravindakshan ◽  
Marykutty Thomas ◽  
K. Raji
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Dna Sequencing ◽  
Bone Morphogenetic Protein ◽  
Restriction Site ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Morphogenetic Protein

scholarly journals Homogeneous Real-Time Detection of Single-Nucleotide Polymorphisms by Strand Displacement Amplification on the BD ProbeTec ET System

2003 ◽  
Vol 49 (10) ◽  
pp. 1599-1607 ◽  
Author(s):  
Sha-Sha Wang ◽  
Keith Thornton ◽  
Andrew M Kuhn ◽  
James G Nadeau ◽  
Tobin J Hellyer
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Dna Sequencing ◽  
Real Time ◽  
Whole Blood ◽  
Nucleotide Polymorphisms ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Single Nucleotide ◽  
Buccal Swabs ◽  
Real Time Detection

Abstract Background: The BD ProbeTec™ ET System is based on isothermal strand displacement amplification (SDA) of target nucleic acid coupled with homogeneous real-time detection using fluorescent probes. We have developed a novel, rapid method using this platform that incorporates a universal detection format for identification of single-nucleotide polymorphisms (SNPs) and other genotypic variations. Method: The system uses a common pair of fluorescent Detector Probes in conjunction with unlabeled allele-specific Adapter Primers and a universal buffer chemistry to permit analysis of multiple SNP loci under generic assay conditions. We used Detector Probes labeled with different dyes to facilitate differentiation of two alternative alleles in a single reaction with no postamplification manipulation. We analyzed six SNPs within the human β2-adrenergic receptor (β2AR) gene, using whole blood, buccal swabs, and urine samples, and compared results with those obtained by DNA sequencing. Results: Unprocessed whole blood was successfully genotyped with as little as 0.1–1 μL of sample per reaction. All six β2AR assays were able to accommodate ≥20 μL of unprocessed whole blood. For the 14 individuals tested, genotypes determined with the six β2AR assays agreed with DNA sequencing results. Conclusion: SDA-based allelic differentiation on the BD ProbeTec ET System can detect SNPs rapidly, using whole blood, buccal swabs, or urine.

Quantification of single nucleotide polymorphisms by automated DNA sequencing

2003 ◽  
Vol 309 (2) ◽  
pp. 331-338 ◽  
Author(s):  
Ping Qiu ◽  
George J. Soder ◽  
Vincent J Sanfiorenzo ◽  
Luquan Wang ◽  
Jonathan R. Greene ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Dna Sequencing ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Automated Dna Sequencing

A new resource of single nucleotide polymorphisms in the Japanese eel Anguilla japonica derived from restriction site-associated DNA

2016 ◽  
Vol 63 (4) ◽  
pp. 496-504 ◽  
Author(s):  
Masashi Sekino ◽  
Reiichiro Nakamichi ◽  
Yuki Iwasaki ◽  
Akifumi S. Tanabe ◽  
Atushi Fujiwara ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Restriction Site ◽  
Anguilla Japonica ◽  
Japanese Eel ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

scholarly journals Erratum to: A new resource of single nucleotide polymorphisms in the Japanese eel Anguilla japonica derived from restriction site-associated DNA

2016 ◽  
Vol 63 (4) ◽  
pp. 505-505
Author(s):  
Masashi Sekino ◽  
Reiichiro Nakamichi ◽  
Yuki Iwasaki ◽  
Akifumi S. Tanabe ◽  
Atushi Fujiwara ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Restriction Site ◽  
Anguilla Japonica ◽  
Japanese Eel ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide
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