scholarly journals In vitro acidification potential and fermentation pattern of cereal grains incubated with inoculum of animals given forage or concentrate-based diets

2018 ◽  
Vol 58 (12) ◽  
pp. 2300 ◽  
Author(s):  
Z. Amanzougarene ◽  
S. Yuste ◽  
C. Castrillo ◽  
M. Fondevila
Keyword(s):  
Volatile Fatty Acid ◽  
Gas Production ◽  
Total Volatile ◽  
Maize Varieties ◽  
Fermentative Activity ◽  
Fermentation Pattern ◽  
Acidification Potential ◽  
Branched Chain ◽  
Gas Volume

This work aimed to study the acidification and fermentation pattern of maize (M1, M2, M3), barley (B1, B2, B3) and sorghum (S1, S2, S3) varieties depending on the rumen environment (inoculum from forage or concentrate diets, FI or CI), in 10 h incubation series with a low buffered medium. With CI, gas volume from barley was similar to maize (P > 0.05) except at 4 and 6 h, when M2 and M1 were lower. Barley or maize varieties did not differ in gas production (P > 0.05). After 10 h, barley and maize produced on average 82.5 and 73.0 mL/g organic matter (OM), whereas S1, S2 and S3 rendered 68.4, 31.1 and 39.7 mL/g. With FI, differences between barley and maize were detected after 6 h (61.0, 35.3 and 14.1 mL/g OM at 10 h for barley, maize and sorghum). Among inocula, pH with CI was lower than with FI (P < 0.001). Incubation pH at 10 h was 5.51, 5.21 and 5.00 with CI, and 5.52, 5.85 and 5.91 with FI, for sorghum, maize and barley varieties. Gas production and total volatile fatty acid (VFA) concentration were higher (P < 0.001) with CI than FI (67.3 vs 36.8 mL/g and 31.0 vs 19.9 mmol/L at 10 and 8 h). Butyrate and valerate proportions were higher with CI (P < 0.001), whereas acetate and branched chain VFA were lower (P < 0.001). Fermentative activity against vitreous starch such as maize or sorghum was lower for forage than concentrate inoculum. Study of fermentation of starch-rich substrates in a low buffered medium gives a more realistic picture than conventionally buffered conditions.

scholarly journals Effect Of Dietary Concentrate And Bypass Fat Supplements On In Vitro Rumen Fermentation, Digestibility And Rumen Microbial Population In Buffaloes

2020 ◽  
Author(s):  
AMIRUL FAIZ MOHD AZMI ◽  
A. Hafandi ◽  
Y. M. Goh ◽  
MOHD ZAMRI SAAD ◽  
A. B. Md ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Microbial Population ◽  
Nutritional Composition ◽  
Gas Production ◽  
Total Volatile ◽  
Brachiaria Decumbens ◽  
Total Volatile Fatty Acid ◽  
Bypass Fat

Abstract Background: The effect of Brachiaria decumbens supplemented with concentrate and bypass fat ratio 100, 70:30, 70:15:15 (w/w) on nutritional composition, in vitro rumen fermentation and microbial population in Murrah cross and Swamp buffaloes were investigated. Incubation were conducted using rumen fluid obtained from the breed of each buffalo which were fed the same based diet (100% Brachiaria decumbens). For the in vitro fermentation characteristic after 48h incubation, total gas production, pH, total volatile fatty acid (TVFA), apparent rumen degradable carbohydrate (ARDC), methane (CH4) and ammonia (NH 3) were determined. The molecular technique also was used to quantify rumen total bacteria, total protozoa, total methanogens, Fibrobacter succinogens and Ruminococcus albus.Results: The results revealed Diet C showed significantly highest in dry matter, crude fat, metabolized energy and optimum in crude protein and carbohydrate value while lower in crude fiber as compared to Diet B and Diet A (P<0.05). In both breed were showed parameters of gas production, total volatile fatty acid (TVFA) and its proportion, and total microbial population were increased parallelly with the increase of 30% concentrate levels in Diet B, while Diet C was moderate with the presence of 4% bypass fat and 26% of concentrate (P<0.05). The methane concentration as well the total methanogens population increased significantly (P<0.05) in Diet B when compared among other diets, but no significant difference was found when comparing between buffalo species. Meanwhile, pH value was slightly decreased with the dietary supplementation in both breeds, but the population of cellulolytic bacteria was not affected.Conclusions: This study showed that dietary concentrate and bypass fat supplementation had improved nutritional composition, in vitro fermentation characteristics by increasing VFA concentration, altering total microbial population, and potentially used as new diet for buffaloes in Malaysia.

scholarly journals Fermentation Pattern of Several Carbohydrate Sources Incubated in An in Vitro Semicontinuous System with Inocula From Ruminants Given Either Forage or Concentrate-Based Diets

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 261 ◽  
Author(s):  
Zahia Amanzougarene ◽  
Susana Yuste ◽  
Manuel Fondevila
Keyword(s):  
Rumen Fluid ◽  
Barley Straw ◽  
Lactic Acid Concentration ◽  
Total Volatile ◽  
Microbial Structure ◽  
Rumen Ph ◽  
Citrus Pulp ◽  
Fermentation Pattern ◽  
Gas Volume

The fermentation pattern of several carbohydrate sources and their interaction with the nature of microbial inoculum was studied. Barley (B), maize (M), sorghum, (S), sugarbeet pulp (BP), citrus pulp (CP) and wheat bran (WB) were tested in an in vitro semicontinuous system maintaining poorly buffered conditions from 0 to 6 h, and being gradually buffered to 6.5 from 8 to 24 h to simulate the rumen pH pattern. Rumen fluid inoculum was obtained from lambs fed with either concentrate and barley straw (CI) or alfalfa hay (FI). The extent of fermentation was higher with CI than FI throughout the incubation (p < 0.05). Among the substrates, S, BP and M maintained the highest pH (p < 0.05), whereas CP recorded the lowest pH with both inocula. Similarly, CP recorded the highest gas volume throughout the incubation, followed by WB and B, and S recorded the lowest volume (p < 0.05). On average, the total volatile fatty acid (VFA), as well as lactic acid concentration, was higher with CP than in the other substrates (p < 0.05). The microbial structure was more affected by the animal donor of inoculum than by the substrate. The in vitro semicontinuous system allows for the study of the rumen environment acidification and substrate microbial fermentation under intensive feeding conditions.

Determining the nutritive value of some commercial pulse screenings using in sacco and in vitro methods

2020 ◽  
Vol 60 (3) ◽  
pp. 379
Author(s):  
Ghodrat Mohammadi ◽  
Mostafa Malecky ◽  
Jamal Seifdavati
Keyword(s):  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Nutritive Value ◽  
Gas Production ◽  
Total Volatile ◽  
Volatile Fatty Acid Concentration ◽  
Ruminal Degradability ◽  
Total Volatile Fatty Acid ◽  
In Sacco

The present study aimed at determining in sacco and in vitro the nutritive value of lentil screenings (LS), common vetch screenings (CVS) and bitter vetch screenings (BVS). For this purpose, three experiments (Exp.) were conducted. Ruminal degradation characteristics of the screenings were determined in Exp. 1 by the nylon bag technique using three ruminally fistulated rams. Gas production kinetics, and ruminal degradability and fermentation indices of the screenings were determined using 144 and 24-h incubations, respectively in Exp. 2. In Exp. 3, a basal growing ration for lambs (control) was supplemented by 175 or 350 g/kg of either LS, CVS or BVS (LS175, LS350, CVS175, CVS350, BVS175 and BVS350 respectively), and ruminal degradability and fermentation of the rations were evaluated in vitro. Soluble and potentially degradable fractions of protein were 0.321 and 0.719, 0.362 and 0.688, and 0.333 and 0.707, for LS, BVS and CVS respectively. Metabolisable energy was 11.1, 11.6 and 12.1 MJ/kg DM for LS, BVS and CVS respectively. The screenings had comparable gas production profiles, ruminal digestibility and total volatile fatty acid concentration. However, CVS and BVS had a higher propionate and a lower acetate:propionate ratio than LS (P &lt; 0.05). Including BVS and LS in the mixed ration increased total volatile fatty acid at both inclusion levels, but only BVS enhanced propionate proportion and lowered the acetate:propionate ratio (P &lt; 0.05). These results revealed that these screenings have a good nutritional value and can be used in ruminant diets without adversely affecting the rumen fermentation.

scholarly journals Nutritive value for ruminants of winter oats–legume intercrops in organic cultivation

2014 ◽  
Vol 54 (10) ◽  
pp. 1791 ◽  
Author(s):  
Alexey Díaz ◽  
María Dolores Carro ◽  
Carlos Palacios ◽  
Iván Mateos ◽  
Cristina Saro ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Production ◽  
Volatile Fatty Acid ◽  
Nutritive Value ◽  
Gas Production ◽  
Forage Yield ◽  
Total Volatile ◽  
Fatty Acid Production ◽  
Total Volatile Fatty Acid

Winter oats were grown according to European organic farming regulations in monoculture (oats) and in intercropping with bard vetch (BAV), bitter vetch (BIV) or both legumes (MIX) to evaluate the effects of intercropping on forage yield and nutritive value for ruminants. The experiment was carried out as a randomised complete block design with four replications, and whole forage samples were obtained at two harvest dates (June and July). For both harvest times, all intercrops increased (P < 0.05) forage yield compared with oats, but forage crude protein content was only increased (P < 0.05) for BAV and MIX. Compared with oats, intercropping with BAV increased (P < 0.05) in vitro rate of gas production and total volatile fatty acid production, indicating a higher rate and extent of rumen degradation of BAV forage. In contrast, BIV forage harvested in June had lower (P < 0.05) rate of gas production and total volatile fatty acid production than June oats, but in general no differences in the in vitro rumen fermentation were detected between oats and BIV samples harvested in July. The results indicate that forage yield and quality can be enhanced by intercropping oats with BAV; however, intercropping with BIV increased yield but decreased nutritive value of the forage.

scholarly journals In vitro fermentation pattern and acidification potential of different sources of carbohydrates for ruminants given high concentrate diets

2017 ◽  
Vol 15 (2) ◽  
pp. e0602 ◽  
Author(s):  
Zahia Amanzougarene ◽  
Susana Yuste ◽  
Antonio De Vega ◽  
Manuel Fondevila
Keyword(s):  
Gas Production ◽  
In Vitro Fermentation ◽  
Practical Applications ◽  
Citrus Pulp ◽  
Fermentation Pattern ◽  
Acidification Potential ◽  
Maize Grain ◽  
Fibrous Diet ◽  
Different Sources

The in vitro fermentation pattern of five sources of carbohydrates of differing nature (maize grain, MZ; sucrose, SU; wheat bran, WB; sugarbeet pulp, BP; and citrus pulp, CT) under conditions of high concentrate diets for ruminants was studied. A first 8 h incubation trial was performed under optimal pH using inoculum from ewes given a fibrous diet, to compare fermentative characteristics of substrates. As planned, incubation pH ranged within 6.3 to 6.6. The gas produced from CT was higher than MZ, SU and BP from 4 and 6 h onwards, and at 8 h, respectively (p<0.05). There were no differences (p>0.05) on total volatile fatty acid (VFA) concentration, nor on acetate or propionate proportions, but butyrate was lowest (p<0.05) with CT and BP. The second incubation trial was performed in a poorly-buffered medium, with inoculum from ewes given a concentrate diet. All substrates showed a gradual drop of pH, being lowest with SU after 4 h (p<0.05). Throughout the incubation, gas production was highest with CT and lowest with MZ and BP (p<0.05). Total 8 h VFA concentration was higher with CT than BP, SU and MZ (p<0.05). Acetate proportion was higher, and that of propionate lower, with BP than WB (p<0.05), butyrate proportion being higher with MZ and WB than with BP and CT (p<0.05). Lactic acid concentration was higher (p<0.05) with SU than WB and BP. Fermentation characteristics and acidification potential of feeds depend on the nature of their carbohydrate fraction, and must be considered for practical applications.

scholarly journals Associative Effects between Forages and Concentrates on In Vitro Fermentation of Working Equine Diets

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2212
Author(s):  
Mónica Gandarillas ◽  
Juan Pablo Keim ◽  
Elisa María Gapp
Keyword(s):  
High Performance ◽  
Volatile Fatty Acid ◽  
Animal Species ◽  
Nutritive Value ◽  
Gas Production ◽  
In Vitro Fermentation ◽  
In Vitro Gas Production ◽  
Production Experiment ◽  
Need To Evaluate

Background: Horses are hindgut fermenters, and it is therefore important to determine the postgastric nutritive value of their feedstuffs and diets. Moreover, it has been demonstrated in other animal species that the fermentation of diets results in different values than those expected from pure ingredients. Therefore, the general objective of this work is to evaluate the gas production (GP) and volatile fatty acid (VFA) concentration, as well as the associative effects, of mixtures of different forages and concentrated foods, which are representative of the traditional diets of high-performance horses. Methods: An in vitro gas production experiment was conducted to assess the fermentation of two forages and three concentrates that are typical in horse diets. The combination of 70% of forage and 30% concentrates was also assessed to determine potential associative effects. Results: Concentrates and grains produced higher GP and VFA than forages when evaluated alone. When experimental diets were incubated, GP parameters and VFA concentrations of forage–concentrate mixtures had unexpected differences from the values expected from the fermentation of pure ingredients, suggesting the occurrence of associative effects. Conclusions: Our results indicate that there is a need to evaluate the fermentation of diets, rather than predicting from the values of pure ingredients.

Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques

2005 ◽  
Vol 123-124 ◽  
pp. 107-118 ◽  
Author(s):  
Gonzalo Hervás ◽  
Pilar Frutos ◽  
F. Javier Giráldez ◽  
Manuel J. Mora ◽  
Begoña Fernández ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Gas Production ◽  
In Vitro Gas Production ◽  
Production Techniques ◽  
Fermentative Activity

The need to complement in vitro gas production measurements with residue determinations from in sacco degradabilities to improve the prediction of voluntary intake of hays

1997 ◽  
Vol 64 (1) ◽  
pp. 71-75 ◽  
Author(s):  
M. Blümmel ◽  
P. Bullerdieck
Keyword(s):  
Volume Ratio ◽  
Gas Production ◽  
Published Data ◽  
Fatty Acid Production ◽  
Residue Determination ◽  
In Vitro Gas Production ◽  
Gas Volume ◽  
In Sacco Degradability ◽  
In Sacco

AbstractThe need to complement in vitro gas production measurements with residue determination is demonstrated by the recalculation and reassessment of published data on in vitro gas production, in sacco degradabilities and voluntary dry matter intake (DMI). The in sacco degradability — gas volume ratio was determined at 24 and 48 h of incubation, termed partitioning factor (PF) and combined with rate and extent parameters of in sacco degradability and in vitro gas production to predict DMI. In vitro gas production and in sacco degradability characteristics (a + b) and c as described by the equation y = a + b(1−ect) explained 0·373 and 0·668 respectively of the variation in DMI of 19 legume and grass hays. The complementation of gas production parameters by the PF24 increased the R2 value to 0·744 with PF24 accounting for 0·407 of the variation in DMI, the rate of gas production (c) for 0·218 and the extent of gas production (a + b) for 0·119 of the variation in DMI. As a single parameter, PF48 showed the highest correlation (R2 = 0·597) with DMI but the combination of PF4S with rate and extent of in sacco or in vitro gas production measurements did not improve the correlation further, probably due to an intercorrelation between rates of fermentation and PF4S. Hays which were degraded at faster rates had higher PF values indicating proportionally higher microbial yield and lower short-chain fatty acid production per unit substrate degraded. Generally, hays with high in sacco degradabilities but proportionally low gas production i.e. hays with high PF values showed higher DMI.

Estimation of fermentable energy by the automatic in vitro gas production technique for a selection of feedstuffs incubated alone and in combination

1998 ◽  
Vol 1998 ◽  
pp. 69-69
Author(s):  
S. Fakhri ◽  
A. R. Moss ◽  
D.I. Givens ◽  
E. Owen
Keyword(s):  
Organic Matter ◽  
Incubation Period ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Fe Content ◽  
The Individual ◽  
Gas Volume ◽  
Selection Of

The gas production (GP) technique has previously been used to estimate the gas volume (fermentable energy (FE)) of compound feed ingredients for ruminants (Newbold et al., 1996). It was shown that the FE content of feed mixtures was represented by the combination of the total gas from the incubation of the individual feeds. However this additivity might not be consistent throughout the incubation period. The objectives were to test whether 1. other GP parameters give better estimates of FE for simple mixtures and are they additive; 2. whether organic matter apparently degraded in the rumen (OMADR) explain differences in GP; and 3. to find out if there are any other better measures than OMADR for estimating FE.

scholarly journals The Effect of Multistage Ammoniation on Fiber Fraction and Digestibility of Maize Stover in vitro

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
K. Dakaew ◽  
A. Abrar ◽  
A. Cherdthong
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Total Volatile ◽  
Maize Stover ◽  
Dry Matter Digestibility ◽  
Fiber Fraction ◽  
Total Volatile Fatty Acid

The main objective of this experiment was to assess the effect of multistage ammoniation on fiber fraction and digestibility of maize stover in vitro. Maize stover was treated by a multistage technique of different urea connect ration (8%, 4%, and 2%) and incubation for 14 days at room temperature.Digestibility of NDF, ADF, ADL, hemicellulose, and cellulose, in vitro dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N, total volatile fatty acid (VFA) and pH concentration were observed. However treatment maize stover and multistage ammoniation maize stover were composed by using T-test student as the statistic. There were not significantly different on fiber fraction and digestibility of maize stover. However, multistage ammoniation decreased ADF fraction by 56.67% to45.39%. The DM digestibility of multistage ammoniation on fiber fraction and digestibility of maize stover also increased by 35.18 to 45.91. The total VFA and N-ammonia of multistage ammoniation on fiber fraction and digestibility of maize stover also higher than control.Keywords: Ammoniation, Digestibility, in vitro, Maize stover

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