Effect of β-carotene supplementation on the expression of lipid metabolism-related genes and the deposition of back fat in beef cattle

2017 ◽  
Vol 57 (3) ◽  
pp. 513 ◽  
Author(s):  
Q. Jin ◽  
H. B. Zhao ◽  
X. M. Liu ◽  
F. C. Wan ◽  
Y. F. Liu ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Beef Cattle ◽  
Fatty Acid Synthase ◽  
Average Daily Gain ◽  
Control Level ◽  
Supplementation Period ◽  
Fat Thickness ◽  
Fat Synthesis ◽  
Fat Hydrolysis ◽  
Β Carotene

To evaluate the effects of β-carotene (βC) supplementation on lipid metabolism in the back fat of beef cattle, 120 continental crossbred (Simmental × local Luxi yellow cattle) steers were selected randomly from feedlots and allotted to four groups. Each steer was supplemented with 0, 600, 1200, or 1800 mg/day of βC for 90 days, and then received no βC for 60 days (depletion period). The βC levels significantly increased in steers supplemented with βC (P < 0.01), and then decreased to the control level by Day 150. Back fat thickness decreased slightly with increasing βC supplementation, and significantly differed among groups after supplementation ceased (P < 0.01 on Day 120, P < 0.05 on Day 150). Significant regression relationships between βC supplement level and both βC content in back fat tissue on Day 90 and back fat thickness on Days 90, 120, and 150 were established (P < 0.01). No significant differences in the dry matter intake or average daily gain were detected, but higher net meat percentages were observed in the 1200 and 1800 mg/day βC-supplemented groups compared with the control (P < 0.05). The mRNA expression of two fat synthesis-related genes, acetyl-CoA carboxylase and fatty acid synthase, were downregulated during the supplementation period, but upregulated during the next 60 days when the steers received no βC supplementation. In contrast, the expression of two fat hydrolysis-related genes, hormone-sensitive lipase and adipose triglyceride lipase, were upregulated during the supplementation period and downregulated in the subsequent 60 days. The results showed that βC supplementation suppresses back fat deposition in beef cattle by inhibiting fat synthesis and enhancing fat hydrolysis.

scholarly journals Cardiac dysfunction in β-carotene-15,15′-dioxygenase-deficient mice is associated with altered retinoid and lipid metabolism

2014 ◽  
Vol 307 (11) ◽  
pp. H1675-H1684 ◽  
Author(s):  
Seung-Ah Lee ◽  
Hongfeng Jiang ◽  
Chad M. Trent ◽  
Jason J. Yuen ◽  
Sureshbabu Narayanasamy ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Heart Development ◽  
Fatty Acid Synthase ◽  
Hormone Receptors ◽  
Heart Function ◽  
The Body ◽  
Retinol Binding Protein ◽  
Heart Metabolism ◽  
Nonesterified Fatty Acids ◽  
Β Carotene

Dietary carotenoids like β-carotene are converted within the body either to retinoid, via β-carotene-15,15′-dioxygenase (BCO1), or to β-apo-carotenoids, via β-carotene-9′,10′-oxygenase 2. Some β-apo-carotenoids are potent antagonists of retinoic acid receptor (RAR)-mediated transcriptional regulation, which is required to ensure normal heart development and functions. We established liquid chromatography tandem mass spectrometery methods for measuring concentrations of 10 β-apo-carotenoids in mouse plasma, liver, and heart and assessed how these are influenced by Bco1 deficiency and β-carotene intake. Surprisingly, Bco1−/− mice had an increase in heart levels of retinol, nonesterified fatty acids, and ceramides and a decrease in heart triglycerides. These lipid changes were accompanied by elevations in levels of genes important to retinoid metabolism, specifically retinol dehydrogenase 10 and retinol-binding protein 4, as well as genes involved in lipid metabolism, including peroxisome proliferator-activated receptor-γ, lipoprotein lipase, Cd36, stearoyl-CoA desaturase 1, and fatty acid synthase. We also obtained evidence of compromised heart function, as assessed by two-dimensional echocardiography, in Bco1−/− mice. However, the total absence of Bco1 did not substantially affect β-apo-carotenoid concentrations in the heart. β-Carotene administration to matched Bco1−/− and wild-type mice elevated total β-apo-carotenal levels in the heart, liver, and plasma and total β-apo-carotenoic acid levels in the liver. Thus, BCO1 modulates heart metabolism and function, possibly by altering levels of cofactors required for the actions of nuclear hormone receptors.

scholarly journals Performance of finishing beef cattle fed different diets containing whole-crop maize silage or sugarcane silage

2011 ◽  
Vol 40 (3) ◽  
pp. 682-689 ◽  
Author(s):  
Juliano Roman ◽  
Clóves Cabreira Jobim ◽  
Flávio Dutra de Resende ◽  
Gustavo Rezende Siqueira ◽  
Marcelo Henrique de Faria ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Average Daily Gain ◽  
Subcutaneous Fat ◽  
Live Weight ◽  
Feed Conversion ◽  
Maize Silage ◽  
Subcutaneous Fat Thickness ◽  
Fat Thickness ◽  
Finishing Beef Cattle ◽  
Daily Gain

The objective of the present study was to evaluate the performance of beef cattle in feedlots fed diets containing either whole-crop maize silage or sugarcane silage, and with different formulations. Five diets were evaluated: MSF - diet containing whole-crop maize silage, with fixed formulation during feedlot period; MSV- diet containing whole-crop maize silage, with variable formulation according to feedlot phase; SCSF - diet containing sugarcane silage with fixed formulation; SCSV- diet containing sugarcane silage, with variable formulation; SCSV/MSV- SCSV diet in initial half of fedlot and MSV diet in final half of feedlot period, respectively. It was used a complete random experimental design, with five treatments and three replicates (pens). It was used 285 steers at 36 months of age, with an initial body weight of 362.4 kg. Diets with whole-crop maize silage promoted greater intake of dry matter (10.5 kg and 2.4% BW) compared with diets containing sugarcane silage (10.1 kg and 2.3% BW). There was no effect of the silages on average daily gain (1.348 kg/animal/day) and live weight at slaughter (481.2 kg BW). SCSV/MSV diet promoted the worst feed conversion (8.0 kg DM/kg BW). Animals fed diets composed of whole-crop maize silage presented greater weight of hot carcass, of carcass yield and subcutaneous fat thickness (262.1 kg, 54.1% and 6.2 mm) compared with the animals fed sugarcane silage (252.5 kg, 52.7% and 4.8 mm). The use of sugarcane silage in diets for finishing beef cattle is feasible and the adjustment of formulation during confinement period does not influence performance of the animals. Replacement of sugarcane silage by whole-crop maize silage during the feeding period affects negatively feed conversion.

scholarly journals PSIV-B-28 Late-Breaking: Performance and carcass quality of Nelore bull’s progenies with contrasting traits for precocity, growth and muscularity

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 319-321
Author(s):  
Taiane S Martins ◽  
Juliana Silva ◽  
Lenise Mueller ◽  
Tamyres Amorim ◽  
Annelise Aila G Lobo ◽  
...  
Keyword(s):  
Feed Efficiency ◽  
Average Daily Gain ◽  
Backfat Thickness ◽  
Longissimus Muscle ◽  
Muscle Area ◽  
Final Body Weight ◽  
Fat Thickness ◽  
Nelore Cattle ◽  
Daily Gain

Abstract The goal of this study was to evaluate the performance and the carcass traits of Nelore cattle progenies from bulls selected by contrasting traits for precocity, growth and muscularity, through the Expected Progeny Difference (EPD). One hundred and five Nelore bulls (initial weight of 350kg±15kg) and 20 months of age were confined and fed with same diet (73% of concentrate). Thirty-two animals were selected to create the contrasting groups for precocity, growth and muscularity (16 animals assigned as a low EPD group - LEPD and 16 animals assigned as a high EPD group - HEPD), based on the EPD of their parents. The ribeye area and backfat thickness were performed by ultrasonography of 12–13th rib fat thickness and longissimus muscle area (LMA), as well as rump fat thickness (RF) measurements. Animals were harvested after 100 days and during the deboning, meat cuts were weight for cutting yield. The animals selected for the HEPD group had greater average daily gain (P = 0.006), which can be explained by the higher feed intake (P = 0.006). However, there are no difference between groups for the final body weight (P = 0.254) and feed efficiency (P = 0.715). The LEPD group presented higher dressing percentage (P = 0.028). Although the groups evaluated did not presented difference in LMA (P = 0.329) and weight of longissimus muscle (P = 0.480), the weight of rump displayed heaviest in the HEPD (P = 0.037). There was no difference between groups for RF (P = 0.086). Nevertheless, backfat thickness was higher in HEPD group (P = 0.006). The present study indicates that Nelore cattle progenies, with parents displaying higher potential for precocity, growth, and muscularity, show greater backfat thickness and weightiest of rump than the other genetic backgrounds. Thanks to FAPESP for the scholarship (Grant # 2017/02349–1).

scholarly journals Lipolytic Postbiotic from Lactobacillus paracasei Manages Metabolic Syndrome in Albino Wistar Rats

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 472
Author(s):  
Ali Osman ◽  
Nashwa El-Gazzar ◽  
Taghreed N. Almanaa ◽  
Abdalla El-Hadary ◽  
Mahmoud Sitohy
Keyword(s):  
Metabolic Syndrome ◽  
Lipid Metabolism ◽  
Lipolytic Activity ◽  
Control Level ◽  
Serum Triglyceride ◽  
Positive Control ◽  
Lactobacillus Paracasei ◽  
Total Serum ◽  
Albino Rats ◽  
Total Serum Cholesterol

The current study investigates the capacity of a lipolytic Lactobacillus paracasei postbiotic as a possible regulator for lipid metabolism by targeting metabolic syndrome as a possibly safer anti-obesity and Anti-dyslipidemia agent replacing atorvastatin (ATOR) and other drugs with proven or suspected health hazards. The high DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS [2,2′-azino-bis (3-ethyl benzothiazoline-6-sulphonic acid)] scavenging activity and high activities of antioxidant enzyme such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-px) of the Lactobacillus paracasei postbiotic (cell-free extract), coupled with considerable lipolytic activity, may support its action against metabolic syndrome. Lactobacillus paracasei isolate was obtained from an Egyptian cheese sample, identified and used for preparing the postbiotic. The postbiotic was characterized and administered to high-fat diet (HFD) albino rats (100 and 200 mg kg−1) for nine weeks, as compared to atorvastatin (ATOR; 10 mg kg−1). The postbiotic could correct the disruption in lipid metabolism and antioxidant enzymes in HFD rats more effectively than ATOR. The two levels of the postbiotic (100 and 200 mg kg−1) reduced total serum lipids by 29% and 34% and serum triglyceride by 32–45% of the positive control level, compared to only 25% and 35% in ATOR’s case, respectively. Both ATOR and the postbiotic (200 mg kg−1) equally decreased total serum cholesterol by about 40% and 39%, while equally raising HDL levels by 28% and 30% of the positive control. The postbiotic counteracted HFD-induced body weight increases more effectively than ATOR without affecting liver and kidney functions or liver histopathology, at the optimal dose of each. The postbiotic is a safer substitute for ATOR in treating metabolic syndrome.

67 Current Status and Future Prospective of the Use of Plant Bioactive Compounds in Dairy and Beef Cattle

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 132-132
Author(s):  
Sergio Calsamiglia ◽  
Maria Rodriguez-Prado ◽  
Gonzalo Fernandez-Turren ◽  
Lorena Castillejos
Keyword(s):  
Beef Cattle ◽  
Essential Oils ◽  
Average Daily Gain ◽  
Rumen Fermentation ◽  
Production Performance ◽  
In Vivo Studies ◽  
Current Status ◽  
The Impact

Abstract In the last 20 years there has been extensive in vitro research on the effects of plant extracts and essential oils on rumen microbial fermentation. The main objectives have been to improve energy metabolism through a reduction in methane emissions and an increase in propionate production; and to improve protein metabolism by reducing proteolysis and deamination. While the positive results from in vitro studies has stimulated the release of commercial products based on blends of essential oils, there is limited in vivo evidence on the rumen fermentation and production performance effects. A literature search was conducted to select in vivo studies where information on rumen fermentation and animal performance was reported. For dairy cattle, we identified 37 studies of which 21 were adequate to test production performance. Ten studies reported increases and 3 decreases in milk yield. For beef cattle, we identified 20 studies with rumen fermentation profile and 22 with performance data. Average daily gain improved in 7 and decreased in 1 study. Only 1 out of 16 studies reported an improvement in feed efficiency. Data indicate that out of more than 500 products tested in vitro, only around 20 have been tested in vivo in different mixtures and doses. The use of statistical approaches will allow to describe the conditions, doses and responses in dairy and beef cattle performance. The search for postruminal effects offers another alternative use. Evidence for effects on the intestinal and systemic effects on the immune system and antioxidant status (i.e., capsicum, garlic, eugenol, cinnamaldehyde curcuma, catechins, anethol or pinene), and in the modulation of metabolic regulation (capsicum, cinnamaldehyde, curcuma or garlic) may open the opportunity for future applications. However, stability of the product in the GI tract, description of the mechanisms of action and the impact of these changes on performance needs to be further demonstrated.

scholarly journals Effect of monensin sodium associative to virginiamycin and/or essential oils on the performance of feedlot finished steers

2018 ◽  
Vol 39 (1) ◽  
pp. 261
Author(s):  
Julio Cezar Heker Junior ◽  
Mikael Neumann ◽  
Robson Kyoshi Ueno ◽  
Margarete Kimie Falbo ◽  
Sandra Galbeiro ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Feeding Behavior ◽  
Dry Matter ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Live Weight ◽  
Average Weight ◽  
Apparent Digestibility ◽  
Fat Thickness

The objective of this study was to evaluate the associative effect of monensin sodium to virginiamycin and/or essential oils on performance, consumption of nutrients and dry matter, apparent digestibility, feeding behavior and carcass characteristics of feedlot finished steers. The experiment lasted 106 days with 10 days of adaptation and 96-day trial, and had 32 crosses angus steers, average age 12 months and average weight of 376 kg, divided into 16 stalls, the weighing took place every 21 days and at the end of the experiment. The treatments consisted of the combination of the following additives to the diet included: Monensin sodium, 200 mg day-1 (MO); Monensin sodium, a dose of 200 mg day-1 + essential oil dose of 1.5g day-1 (MO+EO); Monensin sodium, a dose of 200 mg day-1 + virginiamycin, 200 mg day-1 (MO+VI); Monensin sodium, 200 mg day-1 + essential oil dose of 1.5g day-1 + virginiamycin day, 200 mg day-1 (MO+EO+VI), each treatment had four repetitions, where each repetitions consisted of a bay with two animals. The MO+VI association in relation to MO only increase in average daily gain (ADG) of 24.44%, 22.35%, 21.10% and 17.31% in weighing 42, 63, 84 and 96 days, similar the combination of MO+EO+VI which provided an improvement of 21.94%, 13.59%, 15.45% and 14.75% respectively in the same weightings. The daily carcass gain and carcass overall gain were higher in associations MO+VI and MO+EO+VI and provided an average gain of 16.67 kg more compared to MO and MO+EO. In the parameters feed efficiency, dry matter intake and nutrient expressed in kg day-1 and percentage of live weight were not observed differences (P > 0.05) between treatments. Data on apparent digestibility, feeding behavior and carcass characteristics did not show statistical difference between treatment, except for fat thickness which was higher when associated with any of the additives to the MO, and farm weight was higher in associations containing VI. Associating MO+VI or MO+EO+VI proved to be best in this work compared to MO+EO or only MO in the diets of steers in termination.

Placental Accumulation of Triacylglycerols in Gestational Diabetes Mellitus and Its Association with Altered Fetal Growth are Related to the Differential Expressions of Proteins of Lipid Metabolism

2020 ◽  
Author(s):  
Manoharan Balachandiran ◽  
Zachariah Bobby ◽  
Gowri Dorairajan ◽  
Sajini Elizabeth Jacob ◽  
Victorraj Gladwin ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Fetal Growth ◽  
Fatty Acid Synthase ◽  
Regulatory Element ◽  
Acid Oxidation ◽  
Placental Proteins

Abstract Introduction Gestational diabetes mellitus (GDM) exhibit altered placental lipid metabolism. The molecular basis of this altered metabolism is not clear. Altered placental expression of proteins of lipogenesis and fatty acid oxidation may be involved in the placental accumulation of triacylglycerols (TG). The present study was aimed at investigating the differential expressions of placental proteins related to lipid metabolism among GDM women in comparison with control pregnant women (CPW) and to correlate them with maternal and fetal lipid parameters as well as altered fetal growth. Materials and Methods Maternal blood, cord blood, and placental samples were collected from GDM and CPW. The biochemical parameters, glucose, lipid profile and free fatty acids (FFA) were measured. The placental TG content was measured. Differential placental expressions of proteins; phosphatidylinositol-3-kinase (PI3K) p85α, PI3K p110α,liver X receptor alpha (LXRα), sterol regulatory element binding protein1(SREBP1), fatty acid synthase (FAS), stearyl CoA desaturase1 (SCD1), lipoprotein lipase (LPL),Peroxisome proliferator-activated receptor (PPAR)α and PPARγ were analysed by western blotting and immunohistochemistry. Results Placental protein expressions of PI3K p110α, LXRα, FAS, SCD1, and LPL were found to be significantly higher, whereas PPARα and PPARγ were lower in GDM women compared with CPW. The placental TG content and cord plasma FFA were increased in GDM women compared with CPW. The placental TG content positively correlated with Ponderal index of GDM new-borns. Conclusion Differential expressions of placental proteins related to lipid metabolism in GDM might have led to placental TG accumulation. This might have contributed to the fetal overgrowth in GDM.

scholarly journals SGL 121 Attenuates Nonalcoholic Fatty Liver Disease through Adjusting Lipid Metabolism Through AMPK Signaling Pathway

2020 ◽  
Vol 21 (12) ◽  
pp. 4534
Author(s):  
Da Eun Kim ◽  
Bo Yoon Chang ◽  
Byeong Min Jeon ◽  
Jong In Baek ◽  
Sun Chang Kim ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Liver Disease ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Signaling Pathway ◽  
Hepg2 Cells ◽  
Fatty Liver Disease ◽  
Fatty Acid Synthase ◽  
Density Lipoprotein ◽  
Nonalcoholic Fatty Liver

A ginsenoside F2-enhanced mixture (SGL 121) increases the content of ginsenoside F2 by biotransformation. In the present study, we investigated the effect of SGL 121 on nonalcoholic fatty liver disease (NAFLD) in vitro and in vivo. High-fat, high-carbohydrate-diet (HFHC)-fed mice were administered SGL 121 for 12 weeks to assess its effect on improving NAFLD. In HepG2 cells, SGL 121 acted as an antioxidant, a hepatoprotectant, and had an anti-lipogenic effect. In NAFLD mice, SGL 121 significantly improved body fat mass; levels of hepatic triglyceride (TG), hepatic malondialdehyde (MDA), serum total cholesterol (TC), high-density lipoprotein (HDL), and low-density lipoprotein (LDL); and activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In HepG2 cells, induced by oxidative stress, SGL 121 increased cytoprotection, inhibited reactive oxygen species (ROS) production, and increased antioxidant enzyme activity. SGL 121 activated the Nrf2/HO-1 signaling pathway and improved lipid accumulation induced by free fatty acids (FFA). Sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FAS) expression was significantly reduced in NAFLD-induced liver and HepG2 cells treated with SGL 121. Moreover, SGL 121 activated adenosine monophosphate-activated protein kinase (AMPK), which plays an important role in the regulation of lipid metabolism. The effect of SGL 121 on the improvement of NAFLD seems to be related to its antioxidant effects and activation of AMPK. In conclusion, SGL 121 can be potentially used for the treatment of NAFLD.

Effect of feeding barley based diets on animal performance, carcass characteristics and meat quality of crossbred beef cattle with and without Wagyu genetics

1997 ◽  
Vol 77 (4) ◽  
pp. 655-662 ◽  
Author(s):  
P. S. Mir ◽  
D. R. C. Bailey ◽  
Z. Mir ◽  
S. D. M. Jones ◽  
T. Entz ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Meat Quality ◽  
Shear Force ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Barley Grain ◽  
Crossbred Cattle ◽  
Target Weight ◽  
Meat Samples

Growth performance, carcass characteristics and meat quality of European and British crossbred (EBC; no Wagyu genetics; 28 heifers and 30 steers) cattle were compared with crossbred cattle with 75% Wagyu genetics (WC; seven heifers and 14 steers) to determine the influence of Wagyu genetics on marbling grade of beef cattle fed barley-based diets in a factorial design experiment. Weaned calves (250 d average age) were fed, one of two diets (diet 1, 35% barley grain; diet 2, 40% hay cubes on DM basis, with barley silage, protein and vitamin/mineral premix) for 84 d and then fed diet 1 until they weighed 394 to 432 kg. All cattle were finished on an 80% (DM basis) rolled barley diet and slaughtered. Carcasses were graded and samples procured for meat quality and Warner–Bratzler shear force determination. Number of days on backgrounding diets to arrive at target weight (380 kg) was greater (P < 0.05) for the WC cattle, owing to relatively lower ADG, but days on the finishing diet were fewer for these cattle, compared with EBC cattle. Warm carcass yield (dressing percent) was greater (P < 0.05), but backfat depth was lower (P < 0.05) for WC cattle relative to that of EBC cattle, yet proportion of lean meat yield was similar. Eighty three percent of WC cattle carcasses had Canada AAA (small or more) marbling grade compared with 13% for EBC cattle carcasses. Mean shear force of meat samples from EBC and WC cattle was 4.2 and 3.8 kg, respectively. Results indicated that the extent of carcass marbling can be increased by incorporating Wagyu genetics but age at slaughter of WC cattle was 19 d greater than that of EBC cattle and carcass size was reduced. Key words: Average daily gain, carcass characteristics, European and British crossbred, feed to gain ratio, meat quality, Wagyu crossbred cattle

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