The effect of different levels of crude protein on the pathophysiology of Haemonchus contortus infection in 2-year-old Boer dry does under confined conditions

2017 ◽  
Vol 57 (4) ◽  
pp. 719
Author(s):  
T. V. Can ◽  
M. A. Hohenhaus ◽  
P. J. Murray
Keyword(s):  
Serum Protein ◽  
Dietary Protein ◽  
Haemonchus Contortus ◽  
Haemoglobin Concentration ◽  
Basal Diet ◽  
Protein Supplementation ◽  
Total Serum Protein ◽  
Total Serum ◽  
Control Group ◽  
Cell Volumes

The effect of dietary protein supplementation on the pathophysiology of a single Haemonchus contortus infection in 2-year-old Boer dry does under confined conditions was examined. Twenty-four does were randomly confined in individual pens and allocated into three groups balanced by their liveweight. The goats in the Control group were offered a basal diet whereas goats in Treatments 1 and 2 were fed the basal diet plus 25% and 50% dietary protein increments, respectively. Each animal was orally administrated 100 H. contortus L3 larvae/kg liveweight on Day 0 of the experiment. The goat liveweights and FAMACHA scores were recorded at weekly intervals. Packed cell volumes, eosinophil percentage, haemoglobin concentration, total serum protein, globulin, and albumin concentrations were recorded on Days 0 and 14, and then at weekly intervals during the experiments. Faecal egg counts were measured on Days 0, 21, and then at weekly intervals thereafter. Antigen-specific IgA, IgG, and IgM titres were determined from Enzyme-linked immunosorbent assays tests on Days 0, 28, and at the termination of the experiment. The results of this study showed that a single H. contortus L3 infection did not cause pathogenic effects in 2-year-old Boer dry does. Dietary protein supplementation significantly lowered faecal egg count and significantly enhanced IgG titres (P < 0.05). However, the higher protein diets did not have any significant effects on liveweight, packed cell volumes, haemoglobin concentration, biological parameters (total serum protein, albumin, and globulin concentrations), and peripheral antibodies (IgA and IgM titres) between the treatments. Further investigations are required to clarify the role of protein supplementation to control H. contortus infection in goats.

scholarly journals Effect of Quail Egg Administration on Some Liver Function Related Parameters

2017 ◽  
Vol 9 (2) ◽  
pp. 149-152
Author(s):  
Patrick E. ABA
Keyword(s):  
Serum Protein ◽  
Fasting Blood Glucose ◽  
Negative Control Group ◽  
Oral Route ◽  
Negative Control ◽  
Diabetic Rats ◽  
Total Serum Protein ◽  
Total Serum ◽  
Control Group ◽  
Group 5

There are a lot of testimonies on the therapeutic efficacies of quail egg on diabetics and on liver disorders. This study investigated synthetic and conjugatory states of the liver in diabetic rats administered varying concentrations of quail egg solution. Thirty (30) adult male albino Wistar rats were assigned to 5 groups of 6 rats each. Groups 2-5 of rats were injected with alloxan monohydrate intraperitoneally at the dose of 160 mg/kg, while rats in group 1 served as normal control. Upon establishment of fasting blood glucose level above 126 mg/dl, the rats in groups 2-4 were administered 30, 15 and 7.5 mg/ml of quail egg solution respectively for 7 days. Rats in groups 1 and 5 received distilled water (10 ml/kg) each. All treatments were through the oral route. At the end of the 7 days duration of the study, blood samples for serum protein and bilirubin assays were collected. Results indicated that the quail egg administration to alloxanized rats did not alter total serum protein and albumin values, but improved significantly (p<0.5) the conjugated bilirubin values compared to that of the negative control group (group 5). It was concluded that administration of quail egg solution to alloxanized rats aided hepatic conjugatory ability with little or no effect on its synthetic function.

Ratio of Serum Tartrate-Inhibitable Acid Phosphatase to Total Serum Protein in Benign Prostatic Hypertrophy and Prostatic Carcinoma

1992 ◽  
Vol 38 (2) ◽  
pp. 276-277 ◽  
Author(s):  
George Petrovich ◽  
Jasna Ursich-Jankovich ◽  
Zeijko Prijovich
Keyword(s):  
Acid Phosphatase ◽  
Serum Protein ◽  
Prostatic Carcinoma ◽  
Benign Prostatic Hypertrophy ◽  
Prostatic Hypertrophy ◽  
Total Serum Protein ◽  
Total Serum ◽  
Control Group ◽  
Activity Concentrations ◽  
Specific Activities

Abstract The activity concentration and the specific activity (the ratio of enzyme activity to total serum protein) of the tartrate-inhibitable fraction of acid phosphatase [orthophosphoric monoester phosphohydrolase (acid optimum), EC 3.1.3.2; TIAP] were related to benign prostatic hypertrophy and to prostatic carcinoma. As expected, the TIAP activity concentrations assayed in the sera of patients with benign prostatic hypertrophy were within the range of those assayed in normal human sera. In contrast, the specific activities of TIAP determined in the sera of patients with benign prostatic hypertrophy were significantly higher than those determined in the control group. In the sera of prostatic carcinoma patients, both the TIAP activity concentrations and the TIAP specific activities differed significantly (F = 730) from the normal values.

scholarly journals The effect of using Ginger (Zingiber officinale) or parsley seeds (petroselinum sativum) on some of physiologically traits of black

2012 ◽  
Vol 36 (0A) ◽  
pp. 142-150
Author(s):  
Mohammed M. Dakheel
Keyword(s):  
Herbal Medicine ◽  
Blood Sugar ◽  
Serum Protein ◽  
Zingiber Officinale ◽  
Feed Additives ◽  
Physiological Traits ◽  
Total Serum Protein ◽  
Total Serum ◽  
Control Group ◽  
Globulin Level

The study was aimed to know the effect of adding 3% of grind zingiber officinale or 8% ofpetroselinum sativum seeds in the concentrate diet (400g/head/ daily) of the Black Iraqi Does (19does) in some physiological traits. Nineteen healthy weak-medium does were divided into threegroups, the first group of 6 does fed concentrate diet containing zingiber, while in the secondgroup (6) does were fed on a diet containing 8% grind petroselinum sativum seeds, however, theother does kept as control group and fed the concentrate diet free of the herbal medical additivesduring mid December 2009 up to end of May 2010. The results revealed that group, The first andsecond groups were highly significant (p>0.05) in their Hb and PCV during the late months ofthe study, also both feed additives caused significant (p>0.05) increase in WBCs, lymphocyte andmonocytes ratio, while they caused significant decrease in neutrophils compared with the controlgroup. The total serum protein and albumin of Animals of both groups fed herbal medicine werehighly significant (p>0.05) in comparing with the control group. While the globulin level was notaffected, also blood sugar of both groups fed zingiber or petroselinum seeds showed significant(p> 0.05) lower levels during the late months of the study.

scholarly journals Serum 4-Hydroxy-2-nonenal and Induced Nitric Oxide Synthase in Hypertension Patient

2017 ◽  
Vol 2 (1) ◽  
pp. 29-33
Author(s):  
Alaa H. Jawad ◽  
Ali Hammed ◽  
Hadeel Adil ◽  
Zyad Al-Qaisi ◽  
Amamer Redwan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Serum Protein ◽  
Inflammatory Diseases ◽  
Total Serum Protein ◽  
Total Serum ◽  
Control Group ◽  
Markers Of Oxidative Stress ◽  
Oxide Synthase

Hypertension (HT) And related diseases such as chronic kidney disease(CKD) share in that one of the main reasons for them is to increase the oxidative stress, which in turn increases the severity of the disease and exacerbation of symptoms. Reactive molecules produced from oxidative stress, in addition to causing tissue damage by oxidation of biomolecules like DNA, lipids, proteins and sugars; they are lead to the formation of mediators with potent inflammatory effect. The objective of this study was to investigate some markers of oxidative stress in hypertension (HT) and HT with CKD patients in addition to some biochemical parameters related to these diseases. This study involved 84 male subjects aged between (25-65) year equally divided into three groups, first and second one belong to HT and HT with CKD patients respectively from Al-yarmouk Teaching hospital, while the third one for apparently healthy 28 subjects considered as control group. For each subject in the three groups these markers and parameters were evaluated; 4-hydroxy-2-nonenl(4HNE), induced nitric oxide synthase(iNOS), albumin, urea, creatinine ,total serum protein. The results were compared to control; There was a significantly higher (p<0.01) in 4HNE, and iNOS levels in both HT and HT with CKD patients, while serum albumin and Total serum protein shows significantly (p<0.01) lower levels in both groups. The elevation levels of oxidative stress markers may be due to oxidative damage of tissues that caused by these inflammatory diseases. Was concluded that there was a positive relation between oxidation results from these diseases and their developments and suggest increase need to intake of antioxidants as precaution in front of these disease.

Effects of Water Salinity and Dietary Protein Levels on Total Serum Protein and Hematocrit of Rainbow Trout (Salmo gairdneri) Fingerlings

1974 ◽  
Vol 31 (6) ◽  
pp. 1133-1134 ◽  
Author(s):  
Ibrahim H. Zeitoun ◽  
Duane E. Ullrey ◽  
Peter I. Tack
Keyword(s):  
Rainbow Trout ◽  
Serum Protein ◽  
Dietary Protein ◽  
Water Salinity ◽  
Salmo Gairdneri ◽  
Total Serum Protein ◽  
Total Serum ◽  
Protein Levels ◽  
Salinity Water ◽  
Different Levels

Seven separate diets ranging from 30 to 60% protein in 5% increments were fed for 10 wk to duplicate groups of rainbow trout, Salmo gairdneri, fingerlings maintained at 10 and 20‰ salinity. Water salinity and dietary protein concentration did not significantly influence total serum protein. Hematocrit increased substantially with salinity (35.3% for fish at 10 ppt and 39.1% for those at 20 ppt), whereas different levels of dietary protein were of minor consequence.

scholarly journals The Effect of Different Dietary Energy and Protein Sources on Blood Profile of Crossbreed Holstein Dairy Cows Raised in Small Stake Holder Farms

2021 ◽  
Vol 11 (2) ◽  
pp. 307-312
Author(s):  
Mohammad Firdaus Hudaya ◽  
Pradita Iustitia Sitaresmi ◽  
Diah Tri Widayati
Keyword(s):  
Serum Protein ◽  
Soybean Meal ◽  
Basal Diet ◽  
Protein Supplementation ◽  
Blood Cholesterol ◽  
Holstein Cows ◽  
Total Serum ◽  
Blood Profile ◽  
Treatment Groups ◽  
Significant Difference

The study aimed to evaluate the effect of protein and energy supplementation on the biochemical blood parameters in Holstein cows. The effect of energy and protein supplementation used corn and soybean meal was evaluated on biochemical blood profile in three groups of Holstein cows raised in small stakeholder farmers in Yogyakarta from February to May 2020. Thirty multiparous Holstein cows were allocated to three treatment groups, namely T0 in which the cows fed by the basal diet from the local farmer as well as the T1 (3.5% energy and protein supplementation) and T2 (5% energy and protein supplementation), in which the cows were fed by added energy and protein supplementation. The diets designed for the treatment groups were different from the basal diet by adding two additional ingredients which were soybean meal and corn meal in purpose to depress the stress from adaptive feeding. The results showed that the treated cows (T1 and T2) had significantly higher serum concentrations of glucose (T1 = 2.12 ± 0.49 mmol/L, T2 = 1.86 ± 0.40 mmol/L) rather than T0 (0.98 ± 0.48 mmol/L). The total concentration of serum protein and urea in treated cows was significantly lower than those with the basal diet. Total serum protein and urea in T1 were 0.69 ± 1.37 mmol/L and 7.21 ± 1.99 mmol/L, respectively; which they were 0.63 ± 0.06 mmol/L and 7.69 ± 3.07 mmol/L in T2, compared to the T0 which were 0.82 ±0.05 mmol/L and 7.69 ± 3.07 mmol/L, respectively. There was no significant difference in blood cholesterol among all treatment groups. In conclusion, the supplementations that varied in the proportion of energy and protein intake affected some biochemical blood profiles, such as glucose, protein, and blood urea nitrogen.

scholarly journals PSVII-2 Differentially expressed genes and their biological function in skeletal muscle of calves born from cows with or without protein supplementation during mid-gestation

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 165-166
Author(s):  
Elisa B Carvalho ◽  
Letícia P Sanglard ◽  
Karolina B Nascimento ◽  
Javier M Meneses ◽  
Daniel R Casagrande ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Differentially Expressed Genes ◽  
Muscle Development ◽  
Negative Binomial ◽  
Muscle Protein ◽  
Basal Diet ◽  
Protein Supplementation ◽  
Differentially Expressed ◽  
Control Group ◽  
Q Value

Abstract Gestating cows have an increased nutrient demand to meet the needs of developing the fetus and the mid-gestation is a critical period for the fetal skeletal muscle development. The aim of this study was to evaluate the skeletal muscle transcriptome in the progeny as a function of the maternal protein nutrition during mid-gestation. Eleven Tabapuã cows and their male calves were used in this study. In the first third of gestation (0 to 100 days of gestation; dg), all cows were kept on pasture. From 100 to 200 dg, the control group (CTRL; 7 animals) received a basal diet achieving 5.5% crude protein (CP), whereas the supplemented group (SUPPL; 4 animals) received a basal diet plus protein supplementation (40% CP). After 200 dg, all animals received the same diet. Weaning was performed at 205 ± 7.5 days of age and animals were kept on pasture until reaching 240 days of age, when they were transferred to a feedlot. Muscle samples were collected at 260 days of age and RNA was extracted for RNA-seq analysis. Gene expression data was analyzed with a negative binomial model to identify (q-value ≤ 0.05) differentially expressed genes (DEG) between treatments. A total of 716 DEG were identified (289 DEG up-regulated and 427 down-regulated in SUPPL group; q-value ≤ 0.05). From the 10 most significant down-regulated DEG in the SUPPL group, two genes associated with apoptotic process were identified: MAPK8IP1 and GRINA, with log2 Fold-Changes (log2FC) of 1.04 and 0.49, respectively. From the 10 most significant up-regulated DEG in the SUPPL group, mTOR was identified, with log2FC=0.31. This is a well-known gene involved in muscle protein synthesis. In conclusion, maternal protein supplementation during mid-gestation affects the expression of genes related to energy metabolism and muscle development, which can lead to long-term impacts on production efficiency.

PARALLEL STUDIES OF COMPLEMENT AND COAGULATION IV. EFFECT OF CARBON TETRACHLORIDE

1951 ◽  
Vol 29 (2) ◽  
pp. 48-58
Author(s):  
Christine E. Rice ◽  
Paul Boulanger ◽  
P. J. G. Plummer
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Serum Protein ◽  
Guinea Pigs ◽  
Fatty Degeneration ◽  
Blood Groups ◽  
Total Serum Protein ◽  
Total Serum ◽  
Coagulation Time ◽  
Complement Activity

To determine whether liver injury would result in a parallel decline in the complement titer and coagulative properties of the blood, groups of guinea pigs were given series of injections of the liver poison, carbon tetrachloride. Marked fatty degeneration of the liver, a decline in total serum protein and albumin, a decrease in complement activity, and a prolongation of coagulation time was observed in the treated animals. A general relationship was noted between the albumin-globulin ratio and the complement titer of the serum and between the complement titer and the coagulation time of the plasma.

Muscle Weight Relationship to Serum Proteins, Hemocytes, and Hepatopancreas in the Lobster, Homarus americanus

1967 ◽  
Vol 24 (11) ◽  
pp. 2339-2354 ◽  
Author(s):  
James E. Stewart ◽  
John W. Cornick ◽  
Diane M. Foley ◽  
M. F. Li ◽  
C. M. Bishop
Keyword(s):  
Serum Protein ◽  
Physiological Condition ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Experimental Conditions ◽  
Muscle Weight ◽  
Beef Liver ◽  
The Relationship

Total serum protein values, hemocyte numbers, and muscle weights were determined for 216 intermolt lobsters immediately after their capture, and for 230 others held captive under a variety of dietary and environmental conditions. Average muscle values ranged from approximately 13% to the more normal 20–25% of the live animals' weight, depending upon experimental conditions. The total serum protein up to a level of 55 mg/ml was shown to be a reliable indicator of muscle weights, although the relationship was not identical for all lobster groups. It appeared to be modified chiefly by the areas from which the different groups were taken. Diet was more important than the temperatures (5 to 14 C) in affecting changes in muscle and serum protein values. Starvation caused a greater reduction (50 to 70%) in the size of the hepatopancreas than in the muscle. Histological examination of the hepatopancreatic tissue showed that the lipid content was markedly reduced upon starvation and that a degeneration of this organ was apparent for lobsters fed a beef liver and herring diet. Measurement of serum proteins would appear to be a useful technique in experiments on lobster nutrition and have value, within specified limits, for assessing the physiological condition of wild lobsters.

scholarly journals Studies on the toxicity of an aqueous extract of the leaves of Abrus precatorius in rats

2007 ◽  
Vol 74 (1) ◽  
Author(s):  
A.A. Adedapo ◽  
O.A. Omoloye ◽  
O.G. Ohore
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Aqueous Extract ◽  
Haemoglobin Concentration ◽  
Total Serum Protein ◽  
Total Serum ◽  
Blood Cell Count ◽  
Abrus Precatorius ◽  
White Rats ◽  
Histopathological Studies

The toxic effects of an aqueous extract of Abrus precatorius were studied in 20 male white rats over a period of 18 days. The rats were divided into four groups of five rats per group. Those in Group A served as controls while the rats in Groups B, C and D were dosed per os with 400 mg/kg, 800 mg/kg and 1 600 mg/kg of the extract, respectively. Blood samples were collected for haematological and biochemical analysis and specimens of the liver, kidney and testes were taken for histopathological studies. The study showed that the extract of A. precatorius caused decreased levels of packed cell volume, haemoglobin concentration, red blood cell count, white blood cell count, mean corpuscular volume and mean corpuscular haemoglobin. The extract also resulted in increased levels of total serum protein, albumin, alanine amino transaminase, aspartate amino transferase, alkaline phosphatase and total bilirubin. Histologically, testicular degeneration characterized by decreased numbers of lining cells of the epithelium as well as reduction in sperm cells with presence of scattered Sertoli cells were noted. The study thus showed that aqueous extract of Abrus precatorius is toxic and caution should be exercised in its use for medicinal purpose.

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