Effect of temperature and pre-incubation time of fibrolytic enzymes on in vitro degradability of Brachiaria (Brachiaria decumbens)

2014 ◽  
Vol 54 (10) ◽  
pp. 1779 ◽  
Author(s):  
D. Dineshkumar ◽  
A. L. Abdalla ◽  
S. C. M. L. Silva ◽  
R. C. Lucas ◽  
S. E. A. S. Cavalcante ◽  
...  
Keyword(s):  
Incubation Time ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Productive Efficiency ◽  
Effect Of Temperature ◽  
Brachiaria Decumbens ◽  
Nutrient Excretion ◽  
Fibrolytic Enzymes ◽  
Forage Digestibility

The ruminant production system in Brazil is based mostly on grazing and relies on native pastures and cultivated grass pastures. Improving forage digestibility is essential to overcome the energy loss and excessive nutrient excretion by livestock. Use of exogenous fibrolytic enzymes on tropical grasses has shown promise in increasing forage utilisation and productive efficiency of ruminants. The present study was taken up to evaluate the effect of temperature and pre-incubation time of cellulose-treated substrate (CTS) and xylanase-treated substrate (XTS) on in vitro degradability of Brachiaria decumbens substrate. Two commercial fibrolytic enzymes, cellulase and xylanase, were applied at the manufacturer’s recommended dose of 7.5 and 0.46 enzymatic units per 500 mg DM, respectively. Effect of temperature regimes of substrate exposure (18°C, 25°C and 32°C) and pre-incubation times with the enzymes (0, 12 and 24 h) were investigated in a complete factorial design. Three adult rumen-cannulated Santa Inês sheep served as inoculum donors. Substrates were incubated in vitro in semi-automatic gas production (GP) system and blanks were included for each inoculum. The GP was calculated, rumen degradability and volatile fatty acids (VFA) were determined. Interaction effect between temperature of substrate exposure and pre-incubation time with the enzyme was significant on degradable neutral detergent fibre (DNDF, g/kg) for both CTS (P = 0.01) and XTS (P = 0.04). Net gas production (GP, mL/g DNDF) of XTS (P = 0.06) differ on temperature versus pre-incubation time, whereas CTS were similar (P = 0.34). Partition factor, total VFA and acetate : propionate ratio were non-significant for both the enzymes. These findings highlight that Brachiaria may respond well to exogenous application of cellulase at all the environmental conditions studied and does not need any pre-incubation period.

scholarly journals Effects of addition of exogenous fibrolytic enzymes on digestibility and milk and meat production – a systematic review

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Deli Nazmín Tirado-González ◽  
Gustavo Tirado-Estrada ◽  
Luis Alberto Miranda-Romero ◽  
Rodolfo Ramírez-Valverde ◽  
Sergio Ernesto Medina-Cuéllar ◽  
...  
Keyword(s):  
Systematic Review ◽  
Milk Yield ◽  
Dry Matter ◽  
Production Efficiency ◽  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Gas Production ◽  
Meat Production ◽  
Fibrolytic Enzymes

Abstract Exogenous fibrolytic enzymes (EFE) added to the ruminant diet can increase fiber digestibility and production efficiency. A systematic review was conducted to understand the interactions between EFE and diet on digestibility and animal performance. The database included variables from 384 experiments with EFE and 264 controls from 85 papers published since 2000 (classification criteria: 1) type of study (in vitro, in situ, in vivo), 2) type of ruminants (sheep, Buffaloes, goats, beef and dairy cattle), 3) primary EFE activity (Cellulases (Cel) or Xylanases (Xyl)), 4) forage proportion (FP), 5) type of plant (TP: legumes or grasses), 6) number of ingredients in diets, and 7) application time (AT)). In over of 52.85% of cases, EFE improved the degradability of dry matter (DMD), neutral and acid detergent fiber (NDFD and ADFD), in vitro gas production (GP), volatile fatty acids (VFA), the acetate: propionate ratio (A:P ratio), protein and fat milk, milk yield and average daily gain (ADG) (by 7.78–21.85%). Cel improved organic matter degradability (OMD), GP, VFA, milk yield, and milk protein and fat content. EFE in FP≥40% diets enhanced the ADG, and in grass-based diets increased the dry matter intake (DMI). The AT of EFE affected the DMD, NDFD, and ADFD. Significant correlations were found between the improvements of NDFD or ADFD with DMD (r>0.59), milk yield (r=0.64), and ADG (r=0.59). In conclusion, many factors interact with EFE supplementation effects, but EFE consistently enhanced the DMD, NDFD, and ADFD of ruminant diets, which are related to improvements in productive performance.

scholarly journals Influence of adding fibrolytic enzymes on the ruminal fermentation of date palm by-products

2019 ◽  
Vol 62 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Khalil Abid ◽  
Jihene Jabri ◽  
Yves Beckers ◽  
Hela Yaich ◽  
Atef Malek ◽  
...  
Keyword(s):  
Production Rate ◽  
Volatile Fatty Acids ◽  
Date Palm ◽  
Gas Production ◽  
Feed Additive ◽  
Ruminal Fermentation ◽  
Fibrolytic Enzymes ◽  
Palm Fronds ◽  
By Products

Abstract. This study was conducted in order to assess the influence of four doses (0, 0.5, 1, and 2 mg (g dry matter)−1 of commercial fibrolytic enzymes (MAXFIBER-I®, SHAUMANN GmbH, Wahlstedt, Germany) on in vitro fermentation of date palm (Phoenix dactylifera) by-products: date kernels, wasted dates, floral stems, and palm fronds. Rumen contents were obtained from two non-lactating Holstein cows. Enzyme supplementation to by-products was carried out 12 h prior to incubation. Compared to the control, the enzymatic supplementation quadratically increased the extent but not the gas production rate of date kernel fermentation. Indeed, the potential gas production increased notably by 14.8 % with the lowest enzymes dose following recorded gas production after 48, 72, and 96 h of incubation. The estimated organic-matter digestibility, metabolisable energy, and total volatile fatty acids in the incubation fluid tended to be increased with the lowest dose by 7.8 %, 8.4 %, and 13.9 % respectively. For the wasted dates, this feed additive tended to linearly increase the gas production rate of fermentation with the highest dose. On the other hand, this supplementation had no effect on the ruminal fermentation of the floral stems and palm fronds. The exogenous fibrolytic enzymes were more effective on fibrous but not on lignified date palm by-products.

Assessment of fermentation characteristics of ruminant foods using an automated pressure evaluation system

1998 ◽  
Vol 22 ◽  
pp. 190-192 ◽  
Author(s):  
Z. S. Davies ◽  
A. E. Brooks ◽  
G. W. Griffith ◽  
M. K. Theodorou
Keyword(s):  
Evaluation System ◽  
Pressure Transducer ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Gas Production ◽  
Bicarbonate Buffer ◽  
Electronic Pressure ◽  
Forage Digestibility ◽  
Manual Pressure

Gas production from the in vitro digestion of forage with buffered rumen fluid can be measured and used to determine forage digestibility and fermentation kinetics. Rumen micro-organisms ferment carbohydrate to gases (CO2 and CH4) and volatile fatty acids (VFA). The VFA produced also cause CO2 to be released from the C02-bicarbonate buffer. Theodorou et al. (1994) introduced the principle of measuring gas production by pressure increase using an electronic pressure transducer and sealed gas-tight culture bottles. Gases accumulate in the head space of the culture bottles as fermentation proceeds. The gas is measured and then released at regular intervals throughout the fermentation. This procedure was automated (Davies et al., 1995). The automated pressure evaluation system (APES) has advantages over the manual pressure transducer technique (Theodorou et al., 1994) in that it is less labour intensive and has been shown to be more sensitive to food characteristics (Davies et al., 1995). The APES, used in this work, has been improved to include new switches and a filtering system. It has been used here to determine the fermentation characteristics of various ruminant foods: ryegrass, wheat grain and ryegrass silage.

scholarly journals Rumen Methanogenesis, Rumen Fermentation, and Microbial Community Response to Nitroethane, 2-Nitroethanol, and 2-Nitro-1-Propanol: An In Vitro Study

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 479
Author(s):  
Zhenwei Zhang ◽  
Yanlu Wang ◽  
Xuemeng Si ◽  
Zhijun Cao ◽  
Shengli Li ◽  
...  
Keyword(s):  
Real Time ◽  
Incubation Time ◽  
Real Time Pcr ◽  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
Community Response ◽  
Gas Production ◽  
Mcra Gene ◽  
Degradation Rates

Nitroethane (NE), 2-nitroethanol (NEOH), and 2-nitro-1-propanol (NPOH) were comparatively examined to determine their inhibitory actions on rumen fermentation and methanogenesis in vitro. Fermentation characteristics, CH4 and total gas production, and coenzyme contents were determined at 6, 12, 24, 48, and 72 h incubation time, and the populations of ruminal microbiota were analyzed by real-time PCR at 72 h incubation time. The addition of NE, NEOH, and NPOH slowed down in vitro rumen fermentation and reduced the proportion of molar CH4 by 96.7%, 96.7%, and 41.7%, respectively (p < 0.01). The content of coenzymes F420 and F430 and the relative expression of the mcrA gene declined with the supplementation of NE, NEOH, and NPOH in comparison with the control (p < 0.01). The addition of NE, NEOH, and NPOH decreased total volatile fatty acids (VFAs) and acetate (p < 0.05), but had no effect on propionate concentration (p > 0.05). Real-time PCR results showed that the relative abundance of total methanogens, Methanobacteriales, Methanococcales, and Fibrobacter succinogenes were reduced by NE, NEOH, and NPOH (p < 0.05). In addition, the nitro-degradation rates in culture fluids were ranked as NEOH (−0.088) > NE (−0.069) > NPOH (−0.054). In brief, the results firstly provided evidence that NE, NEOH, and NPOH were able to decrease methanogen abundance and dramatically decrease mcrA gene expression and coenzyme F420 and F430 contents with different magnitudes to reduce ruminal CH4 production.

scholarly journals Digestibility and rumen fermentation of a high forage diet pre-treated with a mixture of cellulase and xylanase enzymes

2021 ◽  
Vol 51 (3) ◽  
pp. 399-406
Author(s):  
K. Selzer ◽  
A. Hassen ◽  
A.M. Akanmu ◽  
A.Z.M. Salem
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Poor Quality ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Ruminal Fermentation ◽  
Forage Digestibility

Forages play an important role in ruminant animal production worldwide. Unlocking the nutritional potential of poor-quality tropical forages with fibrolytic enzymes would improve forage digestibility and utilization. Using in vitro and in vivo methods this study investigated the effect of pre-treating Smutsfinger hay for 24 hours with a mixture of fibrolytic enzyme (100% cellulase; 75% cellulase: 25% xylanase; 50% cellulase: 50% xylanase; 25% cellulase: 75% xylanase; 100% xylanase and a control with no enzyme) on ruminal fermentation and digestibility of nutrients by sheep. For in vitro fermentation, dry matter, neutral detergent fibre (NDF) degradability and volatile fatty acids (VFA) were determined with standard procedures. The same treatments were used for an in vivo digestibility trial using Merino sheep in a 6 x 6 Latin square design. Feed intake and total tract digestibility were recorded. Rumen fluid samples were collected daily, preserved, and analysed for VFA. The addition of 100% cellulase enzyme to Smutsfinger hay in vitro increased (P <0.05) NDF degradability and gas production compared with the control and inclusion of 100% xylanase enzyme. Both 100% cellulase and xylanase enzymes significantly reduced in vitro end time fermentation pH. A 50:50 mixture of cellulase and xylanase plus enzyme in vivo, increased acetate, total VFA concentration, and higher NDF and ADF digestibility of the test feed compared with the control. Inclusion of a 50-75% mixture of cellulase and 50-25% xylanase enzymes treatment led to higher gas production and butyrate concentration, decreased ruminal pH and improved nutrient digestibility.

scholarly journals Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 108
Author(s):  
Yichong Wang ◽  
Sijiong Yu ◽  
Yang Li ◽  
Shuang Zhang ◽  
Xiaolong Qi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Acid Content ◽  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Quadratic Response ◽  
In Vitro Rumen Fermentation

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

scholarly journals Preserving Porphyra umbilicalis and Saccharina latissima as Silages for Ruminant Feeding

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1957
Author(s):  
Margarita Novoa-Garrido ◽  
Carlos Navarro Marcos ◽  
María Dolores Carro Travieso ◽  
Eduarda Molina Alcaide ◽  
Mogens Larsen ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Saccharina Latissima ◽  
Porphyra Umbilicalis ◽  
In Vitro Gas Production ◽  
Ruminal Degradability ◽  
Kinetics Of

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and three silage types: unwashed seaweed ensiled without additive; seaweed washed and ensiled without additive; and seaweed washed and ensiled with 4 g of formic acid (FAC) per kg seaweed. Silages were kept for 3 months in darkness at 20 °C. Pre-wilting prevented (p < 0.001) effluent formation and reduced (p ≤ 0.038) the production of NH3-N and volatile fatty acids for both seaweeds. Both pre-wilting and washing increased (p < 0.05) the ruminal degradability of P. umbilicalis silages but not of S. latissima silages. The pH of the FAC-treated silages was below 4.0, but ranged from 4.54 to 6.23 in non FAC-treated silages. DL-lactate concentrations were low (≤23.0 g/kg dry matter) and acetate was the predominant fermentation product, indicating a non-lactic fermentation. The estimated ruminal degradability of the P. umbilicalis and S. latissima silages was as average, 59.9 and 86.1% of that for high-quality rye-grass silages, respectively, indicating a medium-low nutritional value of these seaweed silages for ruminants.

Chemical characterization and in vitro fermentation ofBrassicastraw treated with the aerobic fungus,Trametes versicolor

2011 ◽  
Vol 91 (4) ◽  
pp. 695-702 ◽  
Author(s):  
J. E. Ramirez-Bribiesca ◽  
Y. Wang ◽  
L. Jin ◽  
T. Canam ◽  
J. R. Town ◽  
...  
Keyword(s):  
Mutant Strain ◽  
Trametes Versicolor ◽  
Lignin Degradation ◽  
Volatile Fatty Acids ◽  
Degradation Products ◽  
Chemical Characterization ◽  
Gas Production ◽  
In Vitro Fermentation ◽  
Lignin Degradation Products

Ramirez-Bribiesca, J. E., Wang, Y., Jin, L., Canam, T., Town, J. R., Tsang, A., Dumonceaux, T. J. and McAllister, T. A. 2011. Chemical characterization and in vitro fermentation of Brassica straw treated with the aerobic fungus, Trametes versicolor . Can. J. Anim. Sci. 91: 695–702. Brassica napus straw (BNS) was either not treated or was treated with two strains of Trametes versicolor; 52J (wild type) or m4D (a cellobiose dehydrogenase-deficient mutant) with four treatments: (i) untreated control (C-BNS), (ii) 52J (B-52J), (iii) m4D (B-m4D) or (iv) m4D+glucose (B-m4Dg). Glucose was provided to encourage growth of the mutant strain. All treatments with T. versicolor decreased (P<0.05) neutral-detergent fibre and increased (P<0.05) protein and the concentration of lignin degradation products in straw. Ergosterol was highest (P<0.05) in straw treated with B-52J, suggesting it generated the most fungal biomass. Insoluble lignin was reduced (P<0.05) in straw treated with B-52J and B-m4D, but not with B-m4Dg. Mannose and xylose concentration were generally higher (P<0.05) in straw treated with fungi, whereas glucose and galactose were lower as compared with C-BNS. The four treatments above were subsequently assessed in rumen in vitro fermentations, along with BNS treated with 2 mL g−1of 5 N NaOH. Concentrations of total volatile fatty acids after 24 and 48h were lower (P<0.05) in incubations that contained BNS treated with T. versicolor as compared with C-BNSor NaOH-treated BNS. Compared with C-BNS, in vitrodry matter disappearance and gas production were increased (P<0.05) by NaOH, but not by treatment with either strain of T. versicolor. Although treatment with T. versicolor did release more lignin degradation products, it did not appear to provide more degradable carbohydrate to in vitro rumen microbial populations, even when a mutant strain with compromised carbohydrate metabolism was utilized. Production of secondary compounds by the aerobic fungi may inhibit rumen microbial fermentation.

scholarly journals The nutritional evaluation of forage-based mixed rations in New Zealand using an in vitro gas production technique. 1: analytical survey

2021 ◽  
pp. 1-6
Author(s):  
N.D. Meads ◽  
R. Tahmasbi ◽  
N. Jantasila
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Environmental Science ◽  
Ghg Emissions ◽  
Negative Relationship ◽  
Real Data ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production

Greenhouse gas (GHG) emissions from livestock are an important consideration in environmental science. Estimating GHG production can be problematic at a farm or animal level, and requires controlled conditions to produce real data. An in vitro gas production technique (IVGPT) was developed to evaluate forage-based total mixed rations in digestion kinetics and GHG production. Two hundred and sixty samples of complete mixed rations (MR), which included a pasture component used in commercial lactating dairy herds, were collected around NZ across three calendar years, 2017-2019. Twenty of the 260 samples were 100% total mixed rations (TMR) with no pasture content. The samples were submitted for proximate analysis as well as IVGPT to generate GHG production figures. The results showed an average total gas production (TGP) of 129.82 ml/g dry matter (DM), 78.6% true digestibility (TDMD), 125.06 mg/g DM microbial biomass (MB), 20.16 g CH4/kg DM, and 12.8 MJME/kg DM. The average nutrient composition was dry matter (DM) 31.55%, crude protein (CP) 21.85%, neutral detergent fibre (NDF) 44.35%, and starch 7.03%. The IVGPT CH4 production was negatively correlated to NDF (r=-0.312), ADF (r=-0.193), TGP (r=-0.216), and was positively correlated with TDMD (r=0.250), apparent digestibility (ADMD) (r=0.614), starch (r=0.117) and volatile fatty acids (r=0.538). The MR diet showed a strong positive relationship with ADMD digestibility (P=0.01) and a negative relationship with fibre content (NDF, P=0.01 and ADF, P=0.01). However, CH4 production reduced linearly with increasing TGP (P=0.01). The results indicated that a greater CH4 production may be related to higher digestibility of mixed ration.

scholarly journals Pomegranate seed oil rich in conjugated linolenic acids reduces in vitro methane production

1970 ◽  
Vol 46 (3) ◽  
pp. 325-335
Author(s):  
E. Maleki ◽  
G.Y. Meng ◽  
M. Faseleh Jahromi ◽  
R. Jorfi ◽  
A. Khoddami ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Seed Oil ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Ruminal Fermentation ◽  
Ch4 Production

The objective of this study was to determine the effect of pomegranate (Punica granatum L.) seed oil (PSO) on gas and methane (CH4) production, ruminal fermentation and microbial populations under in vitro conditions. Three treatments consisting of a control diet containing 10 mg tallow (CON); the control diet with 5 mg PSO + 5 mg tallow (MPSO) and the control diet containing 10 mg PSO (HPSO) were compared. Ten mg of the experimental fat/oil samples were inserted into a gas-tight 100 mL plastic syringe containing 30 mL of an incubation inoculum and 250 mg of a basic substrate of a hay/concentrate (1/1, w/w) mixture. In vitro gas production was recorded over 0, 2, 4, 6, 8, 10, 12 and 24 h of incubation. After 24 hours, incubation was stopped, and methane production, pH, volatile fatty acids (VFAs) and microbial counts were measured in the inoculant. Gas production at 4, 6, 8, 10, 12 and 24 h incubation, metabolizable energy and in vitro organic matter disappearance increased linearly and quadratically as level of PSO increased. Furthermore, the 10 mg PSO (HPSO) decreased CH4 production by 21.0% compared with the control (CON) group. There were no significant differences in total and individual VFA concentrations between different levels of PSO, except for butyric acid. After 24 h of incubation, methanogenesis decreased in the HPSO compared with the MPSO and CON treatments. In addition, total bacteria and protozoa counts increased with rising PSO levels, while population methanogenesis declined significantly. These results suggested that PSO could reduce methane emissions, which might be beneficial to nutrient utilization and growth in ruminants.

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