Genetic effects of polymorphisms in the prolactin receptor gene on chicken reproductive traits

2013 ◽  
Vol 53 (10) ◽  
pp. 1088 ◽  
Author(s):  
D. Y. Li ◽  
L. Zhang ◽  
J. S. Trask ◽  
H. L. Xu ◽  
H. D. Yin ◽  
...  
Keyword(s):  
Transmembrane Protein ◽  
Receptor Gene ◽  
Prolactin Receptor ◽  
Reproductive Traits ◽  
Nucleotide Polymorphisms ◽  
Direct Pcr ◽  
Single Strand Conformational Polymorphism ◽  
Single Nucleotide ◽  
Egg Weight ◽  
Pooled Dna

Prolactin receptor (PRLR) is a single transmembrane protein through which prolactin plays a wide variety of physiological roles in vertebrates. Markers of alleles for the PRLR gene were assessed for the association with six reproductive traits (bodyweight at first egg; egg weight at first egg; age at first egg; number of eggs at 300 days of age; bodyweight at 300 days of age; and egg weight at 300 days of age) in a single generation of the Erlang Mountain Chicken. Five single-nucleotide polymorphisms were detected in the PRLR gene by sequencing pooled DNA samples. Genotypes were identified using PCR-single strand conformational polymorphism and direct PCR-sequencing methods. The GLM procedure was used to estimate the association between genotypes and reproductive traits. The results showed that at the P1 locus, individuals with genotype TT had shorter age at first egg and greater number of eggs at 300 days of age than those with genotype CC (P < 0.01). Our findings suggest that the single-nucleotide polymorphism g.-14A > G at P1 locus could be a potential genetic marker for age at first egg and number of eggs at 300 days of age in the Erlang Mountain Chicken and haplotype ATGTT might be advantageous for reproductive traits.

scholarly journals Polymorphisms in the Chicken Growth Differentiation Factor 9 Gene Associated with Reproductive Traits

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Lingbin Liu ◽  
Zhifu Cui ◽  
Qihai Xiao ◽  
Haihan Zhang ◽  
Xiaoling Zhao ◽  
...  
Keyword(s):  
Body Weight ◽  
Single Nucleotide Polymorphisms ◽  
Gene Polymorphisms ◽  
Reproductive Traits ◽  
Missense Mutations ◽  
Nucleotide Polymorphisms ◽  
Rt Pcr ◽  
Single Nucleotide ◽  
Egg Weight ◽  
Growth Differentiation Factor 9

The aim of the study was to investigateGDF9gene polymorphisms and their association with reproductive traits in chicken using DNA sequencing. A total of 279 Dongxiang blue-shelled (DX) chickens and 232 Luhua (LH) chickens were used for validation. We detected 15 single nucleotide polymorphisms (SNPs): nine SNPs were previously unreported in chicken, two were missense mutations, and only three exhibited significant associations with reproductive traits. G.17156387C>T was significantly associated with age at first egg (AFE) and weight of first egg (WFE) in both breeds. Birds carrying the CC genotype exhibited higher AFE and WFE values than those with the TT genotype. The SNP g.17156427A>G exhibited an association with egg weight at 300 days of age (EWTA) in DX but not in LH chickens. The SNP g.17156703A>C affected the AFE and EN (total number of eggs at 300 days of age) in DX chickens. In addition, certain diplotypes significantly affected AFE, BWTA (body weight at 300 days of age), and EN in both breeds. RT-PCR results showed that theGDF9gene was highly expressed in stroma with cortical follicles (STR) and prehierarchal follicles. These results provided further evidence that theGDF9gene is involved in determining reproductive traits in chicken.

Mutations in the leptin receptor gene associated with delayed onset of puberty are also associated with decreased ovulation and lambing rates in prolific Davisdale sheep

2016 ◽  
Vol 28 (9) ◽  
pp. 1318 ◽  
Author(s):  
Jennifer L. Juengel ◽  
Michelle C. French ◽  
Anne R. O'Connell ◽  
Sara J. Edwards ◽  
Avijit Haldar ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Reproductive Traits ◽  
Nucleotide Polymorphisms ◽  
Wild Type ◽  
Single Nucleotide ◽  
Partial Failure ◽  
Delayed Onset ◽  
Service Conception

The aim of this study was to determine if single nucleotide polymorphisms (SNPs) in the leptin receptor (LEPR) gene associated with delayed onset of puberty are associated with changes in other reproductive traits in adult ewes. The ovulation rate of ewes homozygous for the SNPs was ~15% lower (P < 0.001) than either wild-type or heterozygous ewes. First-service conception rate was also affected, being ~12% lower (P < 0. 01) in ewes homozygous for the LEPR SNPs than their wild-type or heterozygous contemporaries. Partial failure of multiple ovulations was also increased (P < 0.01) in ewes that ovulated three ova that were either heterozygous or homozygous for the mutations. Ewes homozygous for the mutations in LEPR had on average 0.2 fewer lambs at mid-pregnancy and at birth compared with the wild-type or heterozygous ewes (P < 0.01). Thus, mutations in LEPR were strongly associated with poorer reproductive performance in Davisdale ewes, which is likely to be linked to both a reduced number of ova available for fertilisation and an increased number of ewes failing to become pregnant. Increased partial failure of multiple ovulations in ewes with high ovulation rates (i.e. 3 or greater) may also contribute to the poor reproductive performance.

scholarly journals Association of non-synonymous SNPs of <i>OPN</i> gene with litter size traits in pigs

2015 ◽  
Vol 58 (2) ◽  
pp. 317-323 ◽  
Author(s):  
T. Kumchoo ◽  
S. Mekchay
Keyword(s):  
Litter Size ◽  
Reproductive Traits ◽  
Snp Markers ◽  
Strong Linkage Disequilibrium ◽  
Nucleotide Polymorphisms ◽  
Large White ◽  
Single Nucleotide ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Acid Exchange

Abstract. Osteopontin (OPN) gene is a secreted phosphoprotein which appears to play a key function in the conceptus implantation, placentation and maintenance of pregnancy in pigs. The objectives of this study were to verify the non-synonymous single nucleotide polymorphisms (SNPs) and their association with litter size traits in commercial Thai Large White pigs. A total of 320 Thai Large White sows were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Three SNPs at c.425G> A, c.573T> C and c.881C> T revealed amino acid exchange rates of p.110Ala> Thr, p.159Val> Ala and p.262Pro> Ser, respectively, and were then segregated. These three SNPs were significantly associated with total number born (TNB) and number born alive (NBA) traits. No polymorphisms of the two SNP markers (c.278A> G and c.452T> G) were observed in this study. Moreover, the SNPs at c.425G> A and c.573T> C were found to be in strong linkage disequilibrium. The association of OPN with litter size emphasizes the importance of porcine OPN as a candidate gene for reproductive traits in pig breeding.

scholarly journals Correlating single nucleotide polymorphisms in the myostatin gene with performance traits in rabbit

2016 ◽  
Vol 24 (3) ◽  
pp. 213 ◽  
Author(s):  
E.M. Abdel-Kafy ◽  
S.F. Darwish ◽  
D. ElKhishin
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Single Nucleotide Polymorphisms ◽  
Untranslated Region ◽  
Carcass Traits ◽  
Reproductive Traits ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Mstn Gene ◽  
Positive Effects

The Myostatin (MSTN), or Growth and Differentiation Factor 8 (GDF8), gene has been implicated in the double muscling phenomenon, in which a series of mutations render the gene inactive and unable to properly regulate muscle fibre deposition. Single nucleotide polymorphisms (SNPs) in the MSTN gene have been correlated to production traits, making it a candidate target gene to enhance livestock and fowl productivity. This study aimed to assess any association of three SNPs in the rabbit MSTN gene (c.713T&gt;A in exon 2, c.747+34C&gt;T in intron 2, and c.*194A&gt;G in 3’-untranslated region) and their combinations, with carcass, production and reproductive traits. The investigated traits included individual body weight, daily body weight gain, carcass traits and reproductive traits. The 3 SNPs were screened using PCR-restriction fragment length polymorphism (RFLP)-based analysis and the effects of the different SNP genotypes and their combinations were estimated in a rabbit population. Additionally, additive and dominance effects were estimated for significant traits. The results found no significant association between the c.713 T&gt;A SNP and all the examined traits. Allele T at the c.747+34C&gt;T SNP was only significantly associated (P&lt;0.05) with increased body weight at 12 wk of age. However, for the SNP residing in the 3’ untranslated region (c.*194A&gt;G), allele G was significantly associated (P&lt;0.05) with increased body weight and high growth rate. Genotype GG at the c.*194A&gt;G SNP also had positive effects on most carcass traits. The estimated additive genetic effect for the c.*194A&gt;G SNP was significant (P&lt;0.05) with most body weight, daily gain and carcass traits. No significant association was obtained between any MSTN SNPs and reproductive traits. In the combinations analysis, regardless of the genotypes of SNPs at c.713T&gt;A and c.747+34C&gt;T, GG at the c.*194A&gt;G SNP correlated with highest values in body weight and daily weight gain. In conclusion, the ‘G’ allele at the c.*194A&gt;G SNP had positive effects on growth and carcass traits and so could be used as a favourable allele in planning rabbit selection. Further population-wide studies are necessary to test the association of the c.*194A&gt;G SNP with carcass traits. We also recommend evaluation of the potential effects of the c.*194A&gt;G SNP on MSTN gene expression.

Characterisation of the melanocortin-1 receptor gene in alpaca and identification of possible markers associated with phenotypic variations in colour

2009 ◽  
Vol 49 (8) ◽  
pp. 675 ◽  
Author(s):  
N. L. Feeley ◽  
K. A. Munyard
Keyword(s):  
Mus Musculus ◽  
Bos Taurus ◽  
Receptor Gene ◽  
Nucleotide Polymorphisms ◽  
Wild Type ◽  
Single Nucleotide ◽  
Melanocortin 1 Receptor ◽  
Mc1r Gene ◽  
Pcr Products ◽  
Phenotypic Variations

The aim of this study was to determine if any correlation exists between melanocortin-1 receptor (MC1R) polymorphisms and skin and fibre colour in alpacas. Primers capable of amplifying the entire alpaca MC1R gene were designed from a comparative alignment of Bos taurus and Mus musculus MC1R gene sequences. The complete MC1R gene of 41 alpacas exhibiting a range of fibre colours, and which were sourced from farms across Australia, was sequenced from PCR products. Twenty-one single nucleotide polymorphisms were identified within MC1R. Two of these polymorphisms (A82G and C901T) have the potential to reduce eumelanin production by disrupting the activity of MC1R. No agreement was observed between fibre colour alone and MC1R genotype in the 41 animals in this study. However, when the animals were assigned to groups based on the presence or absence of eumelanin in their fibre and skin, only animals that had at least one allele with the A82/C901 combination expressed eumelanin. We propose that A82/C901 is the wild-type dominant ‘E’ MC1R allele, while alpacas with either G82/T901 or G82/Y901 are homozygous for the recessive ‘e’ MC1R allele and are therefore unable to produce eumelanin.

Histamine H3 receptor gene variants associated with drug abuse in patients with cocaine use disorder

2020 ◽  
Vol 34 (11) ◽  
pp. 1326-1330
Author(s):  
Iñigo Pallardo-Fernández ◽  
José Ramón Muñoz-Rodríguez ◽  
Carmen González-Martín ◽  
Luis F Alguacil
Keyword(s):  
Drug Abuse ◽  
Single Nucleotide Polymorphisms ◽  
Receptor Gene ◽  
Gene Variants ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Histamine H3 Receptor ◽  
Cocaine Use ◽  
H3 Receptor ◽  
Histamine H3

Background: Preclinical work revealed significant interactions between ligands of the histamine H3 receptor and different drugs of abuse. In the case of psychostimulants, the results reported are somewhat controversial and human data are still scarce, despite the fact that an inverse agonist of the H3 receptor (pitolisant) has reached the market after approval for the treatment of narcolepsy. Aims: We have studied associations between histamine H3 receptor gene variants and cocaine use disorder to increase the knowledge of the possible involvement of histamine H3 receptor in drug abuse. Methods: Seven single nucleotide polymorphisms of the histamine H3 receptor gene were genotyped by using a multiplexing assay in 248 samples of subjects with cocaine use disorder and 500 randomized samples of subjects representative of the Spanish population. Results: The study of the epidemiological information associated to the samples revealed that subjects with cocaine use disorder broadly abused alcohol, tobacco and cannabinoids. Two single nucleotide polymorphisms (rs3787430 and rs74627870) were found significantly associated with the occurrence of addiction and one more (rs13042865) was specifically related to the severity of cocaine dependence within drug abusers. Conclusions: The associations found in this study further extend the hypothesis that histamine H3 receptor function could be relevant in drug abuse in general and cocaine addiction in particular.

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