Using fistulated sheep to compare in sacco and in vitro rumen degradation of selected feeds

2011 ◽  
Vol 51 (11) ◽  
pp. 1015 ◽  
Author(s):  
Abdul Shakoor Chaudhry ◽  
Ruba A. I. Mohamed
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Water Soluble ◽  
Prediction Ability ◽  
In Vitro Method ◽  
Rumen Degradation ◽  
Degradation Data ◽  
Almost All ◽  
In Sacco

Despite its beneficial role in almost all feed evaluation systems, the in sacco method is inconsistent and labour intensive in estimating the degradation of ruminant feeds. This study compared a rumen fluid-based in vitro method with the in sacco method to estimate degradation of 12 feeds comprising six concentrates and six grasses representing three fields during two seasons of winter and spring. Three feed groups (n = 4) were formed by using two grasses (one per season) and two concentrates. These feeds were incubated alongside blanks, in duplicate, for various hours either in sacco in three fistulated sheep or in vitro by using rumen fluid from the same sheep over three periods using two separate 3 × 3 Latin square designs. The in sacco and in vitro data were statistically compared for the effects of sheep, feed group and period on degradation at each incubation time within each method. The degradation data were fitted exponentially to derive water soluble (a), insoluble (b) and degradation rate (c) constants to estimate effective degradability at rumen outflow rate of 0.02 (P0.02) for each feed in each method. These values were statistically compared for variations within and between feed types for each method. The sheep, group and period did not differ significantly (P > 0.05) for feed degradation at most incubation times within any of these two methods. The in vitro method was able to mimic the in sacco degradation of feeds, although the absolute in vitro values were always lower than the in sacco values. The higher in sacco degradation for almost all feeds were partly attributed to the much greater losses of these feeds during their washing with water at 0 h. The in vitro method did identify significant differences (P < 0.05) in dry matter or crude protein degradations between and within different concentrates and grasses in parallel to the in sacco method. The ranking of feeds was comparable for these methods. The in vitro method can be used in parallel to the in sacco method to estimate degradation of various feeds. However, there is a need for coordinated studies to improve the prediction ability of the in vitro method for its adoption to routinely estimate the degradation of ruminant feeds.

scholarly journals Autumn-cut grass silage as roughage component in dairy cow rations. 2. Rumen degradation, fermentation and kinetics

1993 ◽  
Vol 41 (3) ◽  
pp. 221-234
Author(s):  
H. De Visser ◽  
H. Huisert ◽  
A. Klop ◽  
R.S. Ketelaar
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
High Moisture ◽  
Rumen Degradation ◽  
Grass Silage ◽  
Branched Chain ◽  
Gluten Feed

In a 4 x 4 Latin square experiment the effects of DM content and/or the extent of fermentation in grass silages on the pattern of rumen fermentation and rumen kinetics were studied. In a separate study two cows were used to measure the rate of degradation using the dacron bag technique. Four rumen cannulated dairy cows were used to measure rumen fermentation pattern, rumen kinetics were measured in three of these animals. Basal diets (70% of total DM) consisted of maize silage, moist ensiled beet pulp, moist ensiled maize gluten feed, moist ensiled brewers' grains and a concentrate mixture. The remainder of the diet (30% of total DM) was wilted grass silage (WGS), high moisture grass silage with molasses (MGS), high moisture silage with formic acid (FGS) or wilted grass silage with additional water (WW). All diets were fed as complete feeds. pH of the rumen fluid was lower on the MGS and FGS diets. Concentrations of total VFA, acetic acid, ammonia and branched-chain fatty acids (BCFA) were highest on high moisture diets (MGS and FGS). Rates of clearance and digestion of the organic matter (OM) fractions were or showed tendencies towards being negatively influenced by both MGS and FGS, but remained unaffected by WGS and WW. Degradability of the grass silages was influenced by fermentation in the silo (lower digestible fractions and higher soluble fractions), as were rates of degradation (higher). Results of the degradability measured on the basal diet ingredients were in agreement with published literature and showed a strong correlation between OM digestibility in vitro and the undigestible fraction.

Monitoring digestibility of forages for herbivores: a new application for an old approach

2015 ◽  
Vol 93 (3) ◽  
pp. 187-195 ◽  
Author(s):  
L.L. VanSomeren ◽  
P.S. Barboza ◽  
D.P. Thompson ◽  
D.D. Gustine
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Nutrient Supply ◽  
Nutrient Digestibility ◽  
Total N ◽  
In Vitro Method ◽  
Microbial Sources ◽  
In Sacco

Ruminant populations are often limited by how well individuals are able to acquire nutrients for growth, maintenance, and reproduction. Nutrient supply to the animal is dictated by the concentration of nutrients in feeds and the efficiency of digesting those nutrients (i.e., digestibility). Many different methods have been used to measure digestibility of forages for wild herbivores, all of which rely on collecting rumen fluid from animals or incubation within animals. Animal-based methods can provide useful estimates, but the approach is limited by the expense of fistulated animals, wide variation in digestibility among animals, and contamination from endogenous and microbial sources that impairs the estimation of nutrient digestibility. We tested an in vitro method using a two-stage procedure using purified enzymes. The first stage, a 6 h acid–pepsin treatment, was followed by a combined 72 h amylase–cellulase or amylase–Viscozyme treatment. We then validated our estimates using in sacco and in vivo methods to digest samples of the same forages. In vitro estimates of dry matter (DM) digestibility were correlated with estimates of in sacco and in vivo DM digestibility (both P < 0.01). The in vitro procedure using Viscozyme (r2 = 0.77) was more precise than the in vitro procedure using cellulase (r2 = 0.59). Both procedures can be used to predict in sacco digestibility after correcting for the biases of each method. We used the in vitro method to measure digestibility of nitrogen (N; 0.07–0.95 g/g), which declined to zero as total N content declined below 0.03–0.06 g/g of DM. The in vitro method is well suited to monitoring forage quality over multiple years because it is reproducible, can be used with minimal investment by other laboratories without animal facilities, and can measure digestibility of individual nutrients such as N.

scholarly journals In Vitro Effect of Degradation in Sacco in the Rumen on the Anthelmintic Properties of Parkia Biglobosa and Pterocarpus Erinaceus on Haemonchus Contortus

2018 ◽  
Vol 14 (21) ◽  
pp. 87
Author(s):  
Vidjinnangni Fifamè Grâce Nadège Dèdéhou ◽  
Sévérin Babatoundé ◽  
Mahulé Sylvie Hounzangbé-Adoté
Keyword(s):  
Incubation Period ◽  
Haemonchus Contortus ◽  
Parkia Biglobosa ◽  
Larval Migration ◽  
Rumen Degradation ◽  
Pterocarpus Erinaceus ◽  
Almost All ◽  
Vitro Effect ◽  
In Sacco

Parkia biglobosa and Pterocarpus erinaceus are traditionally used to treat various ailments including helminth infections. This study was undertaken to evaluate the effect of the rumen degradability on anthelmintic properties of the two plants and to examine the possible role of tannins and/or polyphenols on these properties. Hydro-acetone extracts of in sacco degradability residues of P. biglobosa pods and P. erinaceus leaves were screened in vitro to determine the possible anti-parasitic effects against eggs and infective larvae of Haemonchus contortus. In addition, the possible involvement of tannins and/or polyphenols was examined by comparing the levels of inhibition of larval migration obtained with the same extracts, after of not addition of PVPP. Extracts of both plants induced significant egg hatch inhibition (p < 0.001). The effects were dose and incubation period dependent. Extracts of both plants have shown again remarkable larval migration inhibition compared to the PBS (p < 0.05). The effect of incubation period was not significant (p > 0.05). These results suggest that the plants did not lose their anthelmintic properties after rumen degradation. The use of the PVPP indicated for almost all of the extracts that tannins and/or polyphenols are largely involved in the effect. Complementary investigations are necessary to understand the metabolism of these plants in the digestive tract of the animals.

scholarly journals Methods to study degradation of ruminant feeds

2008 ◽  
Vol 21 (1) ◽  
pp. 68-81 ◽  
Author(s):  
Ruba Mohamed ◽  
Abdul Shakoor Chaudhry
Keyword(s):  
Animal Welfare ◽  
Rumen Fluid ◽  
Rumen Degradation ◽  
The Past ◽  
Body Tissues ◽  
Dietary Nutrients ◽  
Ruminant Animals ◽  
Potential Use ◽  
In Sacco

Ruman degradation is crucial in the supply of dietary nutrients to meet the nutrient demands of the anaerobic microbes and body tissues of ruminant animals. Therefore, it is essential to study the dynamics of rumen degradation of various feeds before their potential use to formulate nutritious diets for ruminant animals. Amongst many methods that have been used in the past, thein saccomethod has been the most effective method to study rumen degradation. However, this method is undesirable due to its implications for animal welfare and costs. While manyin vitromethods have been tested as possible alternatives to thein saccomethod to study rumen degradation of feeds, they were unable to remove the need to use fistulated animals to obtain rumen fluid. Although solubility, enzyme- and faeces-basedin vitromethods do not require rumen fluid, they still need data from either thein saccomethod or the rumen fluid-basedin vitromethods for comparison and validation. Therefore, there is a need to developin vitromethods that do not require the need to surgically modify ruminants to obtain rumen fluid to study rumen degradation. We review the potentials and problems associated with the existing methods to study rumen degradation and their implications for the animal industry in different situations.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P &lt; 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

Potential of enzymes or a centrifuged rumen fluid to estimate proteolysis of foods

1998 ◽  
Vol 22 ◽  
pp. 120-122
Author(s):  
A. S. Chaudhry
Keyword(s):  
Rumen Fluid ◽  
Food Proteins ◽  
The Past ◽  
Ruminal Digestion ◽  
Selection Of ◽  
In Sacco

The need to develop an in vitro method to simulate ruminal digestion of protein foods has long been recognized. An in vitro method must be more rapid, consistent and convenient than in sacco methods. Purified enzymes have been examined in the past to estimate in vitro degradability of protein foods (Poos-Floyd et al., 1985; Aufrere et al., 1991; Luchini et al., 1996) or their fractions (Chaudhry and Webster, 1994). However, the selection of an appropriate enzyme for a range of foodstuffs remains to be realized. This study examined the potential of two enzymes and a centrifuged rumen fluid (CRF) to estimate proteolysis of food proteins.

scholarly journals Digestion of timothy silage and hay in reindeer

Rangifer ◽  
1998 ◽  
Vol 18 (1) ◽  
pp. 35
Author(s):  
R. Moen ◽  
M. A. Olsen ◽  
Ø. E. Haga ◽  
W. Sørmo ◽  
T. H. Aagnes Utsi ◽  
...  
Keyword(s):  
Food Intake ◽  
Rangifer Tarandus ◽  
Rumen Fluid ◽  
Total Concentration ◽  
Phleum Pratense ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Energy Deficiency

Leafy timothy (Phleum pratense) silage (S), silage mixed with molasses (SM) and hay (H) were fed to nine male reindeer (Rangifer tarandus tarandus) calves in winter to investigate rumen function and digestion. Three calves were given S with 18.5% dry matter (DM), three were given SM (21.9% DM) and three were given H (85.0% DM). The content of water soluble carbohydrates (in % of DM) was 8.2% in S, 16.0% in SM and 8.5% in H. Median (range) daily DM food intake per kg BM was 12.9 (9-2-14.4) g in calves fed S, 19.0 (19-0-21.9) g in calves fed SM and 21.0 (19.2&not;21.1) g in calves fed H. In vivo digestion of S and SM DM ranged from 78.5-83.1% compared to only 69-9-72.9% in calves fed H. In vitro DM digestion (IVDMD) of cellulose (median) incubated for 48 hours in rumen fluid was, however, significantly (F = 0.05) lower in calves fed S (24.4%) compared to calves fed SM (42.2%). Median IVDMD of cellulose (48 hours) in calves fed H was 36.4%. Total concentration of VFA (range) in the rumen fluid from reindeer fed H (99.7-113.6 mM) and was significantly (P&lt;0.05) higher compared to animals fed S (57.7-85.9 mM) or SM (51.4-72.0 mM). Likewise, the pH of the rumen fluid (range) was significantly (P&lt;0.05) lower in reindeer fed H (6.40-6.78) compared to animals fed S (6.97-7.30) or SM (6.79-7.27). Based on this study it is concluded that leafy timothy preserved as hay seems to be more suitable as emergency feed compared to silage. Supplementation of molasses to silage seems to stimulate food intake and ruminal cellulose digestion in reindeer. The lower intake of S compared to SM or H by reindeer may be explained by ruminal energy deficiency.

The use of a novel in vitro technique for the assessment of the ruminal degradation of soya bean meal.

1993 ◽  
Vol 1993 ◽  
pp. 167-167
Author(s):  
A T Chamberlain
Keyword(s):  
Pore Size ◽  
Cold Water ◽  
Latin Square ◽  
Soya Bean ◽  
Base Line ◽  
Artificial Fibre ◽  
Bean Meal ◽  
Soya Bean Meal ◽  
In Sacco

The metabolisabie protein system (AFRC, 1992) requires the measurement of the dynamics of the degradation of protein in the rumen. However the recommended method, using fistulated animals, is slow, expensive and may be considered unacceptable by many in terms of animal welfare. The objective of this work was to develop an in vitro technique for the routine assessment of feed protein degradability.A 101 vessel fitted with an automatic stirrer was filled with modified van Soest media and rumen liquor (pooled from two sheep) and maintained under anaerobic conditions at 39.5°C. Small artificial fibre bags (4.5x3cm, pore size 54 micro-m ±4) were filled with 0.4g of soya bean meal and incubated in the vessel for 3,6,10 and 24 hours using 3 replicates at each time. Bags were washed in running cold water for 30 mins immediately after removal. Four replicates of 3g samples in large Dacron bags (10x7cm, pore size 55 micro-m ±22) were also incubated in sacco in fistulated yearling wethers using a 4x4 Latin square design to assess the base line degradability. In sacco bags were rinsed and frozen after removal and washed in a domestic washing machine at the end of the experiment.

scholarly journals FORMULATION AND OPTIMIZATION OF CELECOXIB NANOEMULGEL

2017 ◽  
Vol 10 (8) ◽  
pp. 353
Author(s):  
Sankha Bhattacharya ◽  
Bhupendra G Prajapati
Keyword(s):  
Ex Vivo ◽  
Skin Irritation ◽  
Scale Up ◽  
Pilot Scale ◽  
Water Soluble ◽  
Thaw Cycle ◽  
Diffusion Enhancement ◽  
Intrinsic Solubility ◽  
Almost All

Objective: The main objective of this experiment was to prepare and optimized celecoxib nanoemulgel. This formulation can be used for acuterheumatoid arthritis patients.Methods: Celecoxib is a poorly water soluble drug. We prepared celecoxib nanoemulgel to improve intrinsic solubility of celecoxib and enhancedeeper permeation throughout the skin. After several screening, the combination of acetonitrile, triacetin, campul 908P was considered for oil phase;acconon MC8-2EP as surfactant, and capmul MCM C-10 as a co-surfactant accordingly. As per Box-Behnken surface design model, optimization wasdone for all the 13 formulations.Results: Based on pseudo ternary plot, it was found that 4:1 Smix ratio was optimum and possessed maximum drug solubility. Further, screeningshown, 0.25-0.75% carbopol-940 can be a stable candidate for hydrogel preparation. Prepared nanoemulsions and hydrogels were admixed to preparenanoemulgel. Based on overlay plot, EG14* formulation was consider as optimum one, and various evaluation parameters were performed along withother formulations. Using Franz diffusion cell, in-vitro diffusion studies was performed. Almost all the formulations produces good qualitative drugrelease profile. The EG14* shown 95.50% drug release after 12th hrs with standard Higuchi plot (R2 value 0.9989). The optimum viscosity was foundto be 521±0.81 mPas at 100 rpm. The appearance of the formulations was milky, yellowish white with expectable pH ranged from 5.8 to 6.7. Theoptimized formulation has good spreadability coefficient, good ex-vivo diffusion enhancement factor (3.03) as compare to marketed gel. Mostly, ourformulations have less skin irritation and higher anti-inflammatory activity (92.56% of inhibition of paw edema for EG14*).Conclusion: From the thermodynamic studies, it was confirmed that EG14* maintained excellent stability profile in various heating-cooling cycle,centrifugation, and freeze-thaw cycle condition. Hence, it can be conclude that, our formulation, can be consider for pilot scale up.

EFFECT OF FORMALDEHYDE TREATMENT OF SOYBEAN MEAL ON RATES OF PROTEIN DEGRADATION AND MICROBIAL PROTEIN CONCENTRATION IN THE BOVINE RUMEN

1983 ◽  
Vol 63 (1) ◽  
pp. 181-190 ◽  
Author(s):  
S. MAHADEVAN ◽  
R. M. TEATHER ◽  
J. D. ERFLE ◽  
F. D. SAUER
Keyword(s):  
Soybean Meal ◽  
Latin Square ◽  
Experimental Period ◽  
Ammonia Concentration ◽  
Bovine Rumen ◽  
Rumen Degradation ◽  
Formaldehyde Treatment ◽  
Highly Correlated

Four rumen-fistulated cows were fed concentrate:corn silage (40: 60) diets in a 4 × 4 latin square design experiment. The diets differed in the soybean meal or soybean meal treated with 0.1, 0.3 or 0.6% (wt/wt) formaldehyde. Rumen contents were collected hourly from 0900 h to 1600 h with an additional sample at half an hour after the 1100 h feeding on 3 days in the second week of each 2-wk experimental period. The maximum ammonia concentration in rumen contents decreased with increasing levels of formaldehyde used to treat the soybean meal. From 1130 to 1200 h the rates of ammonia appearance in the rumen contents were different (P < 0.05) for all four diets. Treatment of soybean meal with between 0.1 and 0.3% (wt/wt) formaldehyde reduced its in vitro degradation by rumen protease by about 50% compared with the untreated control. The rates of in vivo ammonia appearance and in vitro proteolysis were highly correlated (r = 0.966, P < 0.01) between 1130 h and 1200 h. These rates of rumen ammonia appearance reflected the in vivo rate of proteolysis. Both bacterial and protozoal protein content decreased significantly at the higher levels of formaldehyde treatment. There was a negative correlation (r = − 0.63, P < 0.01) between protozoal and bacterial protein. Key words: Formaldehyde, soybean meal, bovine, rumen, degradation, protease

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