Supplements containing Acacia karroo foliage reduce nematode burdens in Nguni and crossbred cattle

2009 ◽  
Vol 49 (8) ◽  
pp. 646 ◽  
Author(s):  
V. Xhomfulana ◽  
C. Mapiye ◽  
M. Chimonyo ◽  
M. C. Marufu
Keyword(s):  
Control Diet ◽  
Average Daily Gain ◽  
Crossbred Cattle ◽  
Leaf Meal ◽  
Acacia Karroo ◽  
Natural Pasture ◽  
Nguni Cattle ◽  
Sunflower Cake ◽  
Dietary Treatments ◽  
And Control

The objective of this study was to compare nematode worm burdens in Nguni and crossbred cattle supplemented with Acacia karroo leaf meal. Eighteen Nguni (274.4 ± 17.34 kg) and 12 crossbred (230.5 ± 14.62 kg) steers aged 19 months were allocated to three dietary treatments: A. karroo leaf meal, sunflower cake and control with no supplement. Steers in all the treatments grazed on natural pasture whereas those on A. karroo leaf meal and sunflower cake diets were given an additional 1.5 kg and 650 g of feed, respectively, to supply 150 g of protein per day for 60 days. Faecal samples collected from the steers’ recta every fortnight were examined for nematode egg types using the modified McMaster technique. At slaughter, the abomasa and intestines were collected, opened and washed for worm identification. Nguni steers supplemented with the A. karroo leaf meal diet had the lowest mean strongyles egg counts and mean total egg counts (P < 0.05). Steers that received the A. karroo leaf meal and sunflower cake diets had lower Haemonchus contortus and Oesophagostomum colombianum worm burdens than those that received the control diet (P < 0.05). Mean strongyles and Strongyloides egg counts, and H. contortus and Oes. colombianum worm counts were negatively correlated with average daily gain and body condition scores (P < 0.05). Generally, Nguni cattle supplemented with A. karroo leaf meal had the lowest egg loads and worm burdens. It was concluded that supplementing cattle with A. karroo could reduce nematode burdens.

scholarly journals Growth indices, intestinal histomorphology and blood profile of rabbits fed probiotics-, and prebiotics-supplemented diets

2021 ◽  
Author(s):  
Chinwe Uchechi Nwachukwu ◽  
Karimat Imam Aliyu ◽  
Emmanuel Olubisi Ewuola
Keyword(s):  
Body Weight ◽  
Control Diet ◽  
Average Daily Gain ◽  
Feed Additives ◽  
Feed Conversion ◽  
Feeding Trial ◽  
Blood Profile ◽  
Serum Biochemical ◽  
Dietary Treatments ◽  
And Control

Abstract In a 12-week feeding trial, 32 rabbits (Chinchilla X New Zealand White; 56 days old; 691±1g body weight) were used to investigate the effect of pro- and pre-biotics as growth enhancer on the growth performance, intestinal mucosal development, haematological and serum biochemical responses of rabbits. The dietary Biotronic® prebiotics and Biovet®-YC probiotics were added at 400mg/kg and 50mg/kg respectively. The rabbits were housed individually and randomly assigned to four dietary treatments (n=8/group; 50:50 bucks to does) including a control diet (diet 1), diet 2 (control+Biotronic® prebiotics), diet 3 (control+ Biovet®-YC probiotics) and diet 4 (control+symbiotics [Biotronic® prebiotics and Biovet®-YC probiotics]). Body weight (BW), average daily gain (ADG), dry matter intake (DMI), and feed conversion ratio (FCR) were monitored. Five rabbits per treatment were used for organ assessment and intestinal histomorphology after feeding trial. Blood samples were collected for haematological and serum biochemical analysis. Results showed that supplementation of Biotronic® prebiotics and symbiotics in rabbit diet significantly (P&lt;0.05) increased final BW and ADG compared to Biovet®-YC probiotic and control diets. Kidney, lung, oesophagus, gastro-intestinal tract, small and large intestines were significantly (P&lt;0.05) influenced by dietary treatments. Ileal mucosal assessment revealed that villus height (VH), villus width, villus density, crypt depth (CD), and VH:CD ratio of rabbits fed Biotronic® prebiotic and symbiotic diets were similar and significantly (P&lt;0.05) higher than those rabbits fed control and Biovet®-YC probiotic diets. Packed cell volume of rabbits fed symbiotic and control diets was significantly (P&lt;0.05) higher than those fed Biotronic® prebiotic and Biovet®-YC probiotic diets. This study suggests that Biotronic® prebiotics and its combination with Biovet®-YC probiotics are good alternative growth promoting feed additives in rabbit nutrition. They improved performance, intestinal development and blood profiles and aid feed digestion, nutrient absorption and utilization in rabbits.

172 Effects of Dietary Valine, Isoleucine, and Tryptophan Supplementations to Diets Containing Excess Leucine from Corn Protein on Growth Performance of Growing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 61-62
Author(s):  
Woongbi Bi Kwon ◽  
Jose A Soto ◽  
Hans H Stein
Keyword(s):  
Block Design ◽  
Control Diet ◽  
Average Daily Gain ◽  
Growing Pigs ◽  
Experimental Unit ◽  
Negative Effects ◽  
Final Body Weight ◽  
Corn Protein ◽  
Dietary Treatments ◽  
Daily Gain

Abstract An experiment was conducted to test the hypothesis that addition of Val, Ile, or Trp alone or in combination will reduce the negative effects of excess Leu in diets for growing pigs. A total of 288 growing pigs (28.6 ± 2.5 kg) were randomly assigned to 1 of 9 dietary treatments in a randomized complete block design. There were 2 barrows and 2 gilts in each pen and 8 replicate pens per treatment. A control diet based on corn and soybean meal and 8 diets based on corn and a high-protein corn product (48% crude protein) with 2 levels of crystalline L-Val (0 or 0.10%), L-Ile (0 or 0.10%), and L-Trp (0 or 0.05%) were formulated. The crystalline L-Val, L-Ile, and L-Trp increased standardized ileal digestible (SID) Val:Lys from 70 to 80%, SID Ile:Lys from 53 to 63%, and SID Trp:Lys from 18 to 23%, respectively. All diets were formulated to contain 1.00% SID Lys and the 8 diets containing corn protein contained 171% SID Leu:Lys. Individual pig weights were recorded at the beginning and at the conclusion of the 28-d experiment. Data were analyzed using the PROC MIXED of SAS with a pen as the experimental unit. Diet was the fixed effect and block and replicate within block were random effects. Results indicated that final body weight and average daily gain were not different between pigs fed the control diet and pigs fed the diet with Val and Trp addition, but greater (P &lt; 0.001) than for pigs fed the diet with Val addition, Ile addition, Trp addition, Val and Ile addition, Ile and Trp addition, or Val, Ile, and Trp addition (Table 1). In conclusion, addition of Val and Trp to diets with excess Leu may prevent negative effects of excess Leu in diets for growing pigs.

scholarly journals 197 Gene-regulation control of muscle fatigue during transportation with rumen-protected methionine supplementation

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 28-28
Author(s):  
Gaston F Alfaro ◽  
Sonia J Moisá ◽  
Soren P Rodning ◽  
Taylor E Novak
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fatigue ◽  
Messenger Rna ◽  
Control Diet ◽  
Average Daily Gain ◽  
Adaptation Period ◽  
Production Chain ◽  
Transportation Simulation ◽  
Gluten Feed ◽  
Dietary Treatments

Abstract Different participants generally located distant among them compose the U.S. beef production chain, therefore shipping is required for cattle at least once in their lifespan. The aim of this study was to determine the effect of Rumen-Protected Methionine (RPM) supplementation on muscle fatigue gene network, creatine synthesis (CKM), and Reactive Oxygen Species (ROS) metabolism after a transportation simulation in a 1.7-miles oval test track. Angus heifers (n = 18) were stratified by body weight (408 ± 64 kg; BW) and randomly assigned to dietary treatments: 1) control diet (CTRL), and 2) control diet + rumen-protected methionine (RPM). After a successful adaptation period to Calan gates, animals received a common diet of Bermuda hay ad libitum and a soy hulls and corn gluten feed based supplement. After 45 days under supplementation, animals were loaded onto a 32 × 7 ft. trailer and transported for 22 hours. Skeletal muscle biopsies, BW and blood samples were obtained on day 0 (Baseline), 43 (Pre-transport, “PRET”), and 45 (Post-transport, “POST”). Heifer’s average daily gain did not differ between Baseline and PRET (P = 0.41). Control heifer’s shrink was 8% of BW while RPM heifers shrink was 10% (P = 0.29). Cortisol level decreased after transportation, but no differences were observed between treatments (P = 0.94). Messenger RNA was extracted from skeletal muscle tissue and gene expression analysis was performed by RT-qPCR. Results showed that AHCY (Creatine synthesis pathway), SSPN (Sarcoglycan complex), DNMT3A (DNA Methylation), and SOD2 (Oxidative Stress-ROS) were upregulated (P &lt; 0.05) in CTRL between baseline and PRET and, decreased between PRET and POST (P &lt; 0.05) while they remained constant for RPM. Furthermore, CKM was not affected by treatments (P = 0.11). In conclusion, muscle fatigue related genes were not affected by RPM. Although, RPM could affect ROS production, after a long-term transportation.

scholarly journals Effects of cassava meal on carcass characteristics and cholesterol of growing pig

2020 ◽  
Vol 46 (2) ◽  
pp. 150-157
Author(s):  
M. A. Adeyemi ◽  
E. O. Akinfala
Keyword(s):  
Control Diet ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Growing Pigs ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Leaf Meal ◽  
Enhanced Production ◽  
Cassava Plant ◽  
Dietary Treatments

Twenty growing pigs with an average initial weight of 20.00 + 0.5 kg were randomly assigned to five dietary treatments in a completely randomized design with two replicates per treatment and two animals in each replicate. Cassava Plant Meal (CPM) prepared from sundried unpeeled cassava tubers, leaves and tender cassava stems, mixed at ratio 3:1 of unpeeled tuber meal to cassava leaf meal plus tender stem meal with the ratio of cassava leaf meal to tender stem meal as 5:1 was included in balanced rations at graded levels of 0, 25, 50, 75 and 100 percent in a fifty-six days feeding trials. The experimental pigs were weighed before and after slaughtering, dressing percent was evaluated and carcass length and back fat depth were measured on treatment basis. Meat samples were collected at the ribs (2nd and v3rd and also 12th and 13th) for cholesterol analysis. The 50 % CPM diet produced the heaviest slaughter weight. There was no significant (p >0.05) difference in the dressing percentage of pigs across dietary treatments although pigs on the control diet dressed well while pigs on 100 % CPM dressed the poorest. The 100 % CPM diet induced the leanest carcass. All the carcass and organ measurements were significantly (p <0.05) affected by dietary treatments. There was non-significant (p >0.05) influence of dietary treatments on total and high density lipoprotein cholesterol although the 50 % CPM diet produced the least and highest values respectively. Low density and very low density lipoprotein cholesterol were significantly (p <0.05) affected by dietary treatments. Replacement of maize by varying levels of cassava plant meal diets enhanced production of leaner carcass.

scholarly journals 180 Evaluation of soluble concentrated yeast product on sow and litter performance

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 105-106
Author(s):  
Robyn Thompson ◽  
Amanda Graham ◽  
Brandon Knopf ◽  
Laura Greiner ◽  
Sangita Jalukar
Keyword(s):  
Control Diet ◽  
Average Daily Gain ◽  
Random Effect ◽  
Body Weight Loss ◽  
Experimental Unit ◽  
Significant Difference ◽  
Yeast Product ◽  
Dietary Treatments ◽  
Cross Fostering ◽  
Daily Gain

Abstract Two hundred and forty sows (Camborough, PIC, Hendersonville, TN) were started on study at 35 d post-breeding through lactation to determine the effects of soluble concentrated yeast product (Celmanax SCP, Arm and Hammer, Princeton, NJ) on sow and litter performance. Sows were fed their respective dietary treatments: 1) Control diet gestation x control diet lactation; 2) 0.01% Celmanax SCP in gestation x control diet lactation; 3) Control diet gestation x 0.02% Celmanax SCP in lactation; and 4) 0.01% Celmanax SCP in gestation x 0.02% Celmanax SCP in lactation. All diets were formulated to meet or exceed NRC (2012) requirements and contained a feed disinfectant. Sows were allowed ad libitum access to feed and water in lactation. Litters were weighed after cross-fostering, 48 h post-farrow (after litter adjustments and cross-fostering) and at weaning. Sows were weighed entering farrowing and at weaning. Data were analyzed as a randomized complete parity block using PROC MIXED of SAS with sow as the experimental unit, treatment as a fixed effect, and parity as a random effect. There were detectable levels of fumonisin (2 ppm), vomitoxin (1.3 ppm) and zearalenone (243 ppb). There were no differences in sow feed intake or body weight loss (P > 0.10). There was a significant difference in treatments 1, 2, 3 and 4 for days between wean to estrus (5.99, 5.12, 4.90, 4.49; P = 0.01) and percent bred within 7 d (86.53, 97.06, 97.85, 100.00; P = 0.01), respectively. Average piglet wean weights (kg) were 5.82, 5.87, 5.93, 6.01; P = 0.02 for treatments 1, 2, 3 and 4 respectively. Furthermore, there were no responses in piglet average daily gain or mortality (P > 0.10). The feeding of the soluble yeast product to sows does appear to improve subsequent breeding and litter performance.

Supplementing 2 g per day bovine lactoferrin from late gestation until weaning did not improve lactation performance of mixed parity sows

2019 ◽  
Vol 59 (12) ◽  
pp. 2191 ◽  
Author(s):  
Fan Liu ◽  
Jordon Hogg ◽  
Susie Kracht ◽  
Chris J. Brewster ◽  
Dave J. Henman ◽  
...  
Keyword(s):  
Survival Rate ◽  
Control Diet ◽  
Average Daily Gain ◽  
Late Gestation ◽  
Commercial Application ◽  
Bovine Lactoferrin ◽  
Lactation Performance ◽  
Large White ◽  
Litter Weight ◽  
Dietary Treatments

Context Lactoferrin is a non-haem binding protein that possesses antimicrobial, antioxidant and anti-inflammatory properties. A previous study showed lactoferrin supplementation from early gestation to weaning improved lactation performance in primiparous sows; however, it is unknown whether the supplementation within a more specified duration (from late gestation to weaning) can improve lactation performance in multiparous sows, which limits its commercial application. Aims This experiment investigated the effects of 2 g/day bovine lactoferrin supplementation from late gestation to weaning on lactation performance in mixed parity sows. Methods Forty-seven primiparous sows and 167 multiparous sows (up to parity 5; Large White × Landrace, PrimeGro Genetics, Corowa, NSW) were allocated to either a Control diet (2 g/day casein) or Lactoferrin supplement diet (2 g/day lactoferrin) when entering the farrowing house in late gestation (101 ± 4.6 days, mean ± s.d.) balanced by parity (2.4 ± 1.80, mean ± s.d.). Sows were housed individually in farrowing crates and fed the experimental diets until weaning (26 ± 2.5 days, mean ± s.d.). Farrowing outcomes and lactation performance were recorded. Key results Lactoferrin supplementation did not affect the number of piglets born alive, number of stillborn piglets, number of mummified fetuses, the percentage of born-light piglets (≤1.1 kg) or piglet pre-fostering survival rate. Cross-fostering within the first 48 h standardised the litter size and litter weight between dietary treatments. Piglet pre-weaning survival rate, litter weight gain, average daily gain of piglets, or coefficient of variation of piglet growth rate was not affected by lactoferrin supplementation. Daily feed intake during lactation, bodyweight and backfat thickness of sows at weaning were similar between the dietary treatments. Conclusions Two grams per day lactoferrin supplementation from late gestation to weaning did not affect lactation performance in mixed parity sows. Implications The effectiveness of lactoferrin supplementation may depend upon the duration of supplementation, which should be optimised in future studies.

Advanced Chelate Technology Based Trace Minerals Improve Growth Performance, Mineral Digestibility, Tibia Characteristics, and Antioxidant Status in Broiler Chickens

2020 ◽  
Author(s):  
Hossein Ali Ghasemi ◽  
Iman Hajkhodadadi ◽  
Maryam Hafizi ◽  
Kamran Taherpour ◽  
Mohammad Hassan Nazaran
Keyword(s):  
Growth Performance ◽  
Broiler Chickens ◽  
Antioxidant Status ◽  
Bone Mineralization ◽  
Control Diet ◽  
Average Daily Gain ◽  
Trace Minerals ◽  
Randomized Design ◽  
Total Antioxidant ◽  
Dietary Treatments

Abstract Background: Compared to the corresponding source of inorganic trace minerals (TM), chelated supplements are characterized by better physical heterogeneity and chemical stability and appear to be better absorbed in the gut due to possibly decreased interaction with other feed components. This study was designed in broiler chickens to determine the effects of replacing inorganic trace minerals (TM) with an advanced chelate technology based supplement (Bonzachicken) on growth performance, mineral digestibility, tibia bone quality, and antioxidant status. A total of 625 male 1-d-old broiler chickens were allocated to 25 pens and assigned to 5 dietary treatments in a completely randomized design. Chelated TM (CTM) supplement was compared at 3 levels to no TM (NTM) or inorganic TM. A corn–soy-based control diet was supplemented with inorganic TM at the commercially recommended levels (ITM), i.e., iron, zinc, manganese, copper, selenium, iodine, and chromium at 80, 92, 100, 16, 0.3, 1.2, and 0.1 mg/kg, respectively, and varying concentration of CTM, i.e., match to 25, 50, and 100 % of the ITM (diets CTM25, CTM50, and CTM100, respectively). Results: All diets, except diet CTM25, increased average daily gain (ADG), European performance index (EPI), and serum total antioxidant capacity compared to the NTM diet (P < 0.05). Broilers fed the CTM100 diet had lowest overall FCR and highest BWG, EPI, tibia ash, zinc, and manganese contents (P < 0.05). The tibia phosphorus content and apparent ileal digestibilities of phosphorus, zinc, and manganese were lower in the ITM group compared with the CTM50 and CTM100 groups (P < 0.05). Broiler chickens fed any of the diets exhibited higher serum glutathione peroxidase and superoxide dismutase activities and lower malondialdehyde level than those fed the NTM diet, where the best values were found for CTM100 treatment (P < 0.05). Conclusions: These results indicate that while CTM supplementation to 25 and 50% of the commercially recommended levels could support growth performance, a totally replacing ITM by equivalent levels of CTM could also improve growth performance, bone mineralization and antioxidant status of broiler chickens under the conditions of this study.

scholarly journals Effect of an extruded linseed diet on meat quality traits in Nero Lucano pigs

2020 ◽  
Vol 49 (6) ◽  
pp. 1093-1103
Author(s):  
S Tarricone ◽  
M.A. Colonna ◽  
F Giannico ◽  
M Ragni ◽  
A Lestingi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Meat Quality ◽  
Fatty Acid Profile ◽  
Control Diet ◽  
Average Daily Gain ◽  
Feed Conversion ◽  
Homogeneous Groups ◽  
Linum Usitatissimum L ◽  
Dietary Treatments ◽  
Physical And Chemical

The study aimed to evaluate the effect of a diet containing extruded linseed (Linum usitatissimum L.) on growth performances, carcass traits and meat quality in Nero Lucano pigs. Sixteen male piglets were weaned at about 50 days of age and divided into two homogeneous groups fed a control diet (C) or a diet containing 3% extruded linseed (EL). The trial lasted 32 weeks and pigs were slaughtered at 9 months of age. Samples from the Longissimus dorsi (Ld) muscle were analysed to assess physical and chemical parameters and intramuscular fatty acid composition. Average daily gain, feed intake and feed conversion ratio were not affected by the diet. Pigs fed the C diet showed a significantly greater slaughter weight in comparison to the EL group (116.50 vs 108.25 kg) Meat pH and chemical composition were not influenced by the diet. No differences were found for shear force (WBS) in raw meat, while cooked meat of the EL group showed a lower WBS value. The EL diet significantly lowered total SFA and increased total PUFA, of both n-6 and n-3 series. Among the n-6, significant differences between dietary treatments were found for linoleic acid. As for the n-3 series, EL feeding significantly enhanced linolenic acid, EPA, DPA and DHA. Feeding EL also significantly lowered the PUFA/SFA and n-6/n-3 ratios and the AI and TI, with benefits for human health. In conclusion, dietary supplementation with 3% EL didn't affect growth performance while it improved the fatty acid profile of meat. Keywords: fatty acid profile, feeding, flaxseed, pork

PSIV-B-27 Effect of feed flavour on the feeding behaviour and kinetics of daily feed intake of lactating high-prolific sows during summer

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 392-393
Author(s):  
Bruno Alexander N Silva ◽  
Simon Eskinazi ◽  
David V Jacob ◽  
Wagner Araújo ◽  
Iara Ataíde ◽  
...  
Keyword(s):  
Feed Intake ◽  
Control Diet ◽  
Climate Conditions ◽  
Voluntary Feed Intake ◽  
Tropical Conditions ◽  
Randomized Experimental Design ◽  
Dietary Treatments ◽  
And Control ◽  
The Impact ◽  
Kinetics Of

Abstract Increasing voluntary feed intake of the sow is a major challenge for nutritionists in tropical climate conditions. The present study aimed to evaluate the impact of the dietary supplementation of a feed flavour for sows during lactation on their feeding behavior and kinetics of daily feed intake under tropical conditions. A total of 60 high-prolific mixed parity sows divided into 10 batches of 6 sows each were used, distributed in a completely randomized experimental design among 2 dietary treatments (0 or 500 g/ton of Krave®, Adisseo). The average meal size was 107 g/meal lower in sows fed the control diet when compared with flavour fed sows. Daily feed intake was greater in the flavored diet than in the control (6,417 vs. 5,362 g/d; P &lt; 0.05). The flavor improved ingestion time (58.4 vs. 46.6 min/ d, respectively flavour and control; P &lt; 0.05). Sows receiving flavor showed a higher nocturnal (704 vs. 583 g/meal) and diurnal (678 vs. 582 g/meal) feed intake. Sows also showed a higher voluntary intake between 00:00 and 10:00 am, which was equivalent to 83% of the total daily feed intake. From a comparison of hourly feed intakes or variations from hour to hour (during 24 h), the nycthemeral pattern of feed intake peaked twice daily for both dietary treatments. However, the size of the peak was greater (P &lt; 0.05) for flavour fed sows when compared with the control at 0400 and 1900 h (Figure 1a). Diets also influenced on kinetics of daily feed intake during the lactation period.Feed intake was higher for the flavour fed sows from d 3 to 8 and from d 10 to 24 (Figure 1b; P &lt; 0.05).In conclusion, the strategic use of a feed flavour to manipulate the sensorial properties of feed is a viable strategy to increase the sows’ voluntary feed intake.

scholarly journals PSXIV-4 Fetal programming effect of rumen-protected methionine on Angus-Simental heifer offspring’s performance and adipogenesis, oxidative stress and DNA methylation gene expression regulation

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 307-308
Author(s):  
Sonia J Moisá ◽  
Gaston F Alfaro ◽  
Paul H Walz ◽  
Soren P Rodning
Keyword(s):  
Milk Production ◽  
Messenger Rna ◽  
Gene Expression Regulation ◽  
Treatment Time ◽  
Control Diet ◽  
Average Daily Gain ◽  
Late Gestation ◽  
Positive Effects ◽  
Ad Libitum ◽  
Dietary Treatments

Abstract An extensive body of literature reported positive effects of Rumen-Protected Methionine (RPM) on dairy cattle; however, its effects are unknown on beef cattle. The objective of this study was to assess the effect of RPM supplementation on Angus-Simmental heifer’s offspring during late gestation, lactation, and after weaning (~65d). Ninety days prior to calving, 22 Angus-Simmental heifers were stratified by body weight (454 ± 85 kg; BW) and randomly assigned to dietary treatments: 1) Control diet (CTRL), and 2) Control diet + RPM (RPM). Dams had access to Bermuda hay ad libitum, and a soy hull:corn gluten based supplement. After weaning, calves were allocated by dietary treatments based on their dam’s treatment. Calves received Bermuda hay ad libitum and early-wean feed supplement after weaning. Offspring’s BW and Longissimus dorsi (LM) muscle samples were obtained on days 0, 25, 50, 100 after weaning. Messenger RNA was extracted from LM for RT-qPCR analysis. There was a tendency for RPM calves to be heavier at birth compared to control calves (P = 0.07). Although, no differences in BW were detected at weaning and after weaning (P = 0.20). Calves average daily gain between birth and weaning was greater for RPM calves as compared to CON (P = 0.02) suggesting a greater milk production, with no differences after weaning (P = 0.53). RT-qPCR results showed a treatment × time interaction for PPARG, LPL, CEBPG, DNMT1, SOD2 and NOS3 (P &lt; 0.05). Furthermore, PPARG had a treatment and a time effect, which was upregulated in RPM calves (P &lt; 0.01) from 50 to 100 days after weaning. In conclusion, dam’s RPM supplementation during late gestation increased offspring’s BW when inside the womb and probably their milk production. Offspring’s RPM supplementation after weaning potentially stimulates an early differentiation of adipocytes in LM samples, detected by PPARG upregulation.

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