The Natriuretic Peptide (Onp-39) From Platypus (Ornithorhynchus anatinus) Venom Promotes Mast Cell Histamine Release.

1998 ◽  
Vol 20 (2) ◽  
pp. 301
Author(s):  
G.M. De Plater ◽  
R.L. Martin ◽  
P.J. Milburn
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Natriuretic Peptide ◽  
Acute Effects ◽  
Vitro Assay ◽  
Ornithorhynchus Anatinus ◽  
Peritoneal Mast Cells ◽  
Release Histamine ◽  
Anp Receptors

In this study we investigated the possibility that the C-type natriuretic peptide from platypus venom (ONP-39) contributes to the acute effects of envenomation, which include oedema, pain and erythema. These effects may result from the release of auto pharmacological mediators from mast cells. Using an in vitro assay we have demonstrated that both ONP-39 and the endogenous C-type natriuretic peptide (CNP-22) release histamine from rat peritoneal mast cells, an effect similar to the structurally homologous atrial natriuretic peptide (ANP) (Opgenorth et al., 1990, Peptides 11(5):1003-7). Two synthetic peptides, ONP-39(1-17) and ONP-39(18-39), corresponding to the N- and C- termini respectively, are equipotent, suggesting that ONP-39 and other natriuretic peptides do not act through conventional ANP receptors on mast cells. The ability of ONP-39 to promote histamine release suggests that it may contribute to the acute symptoms of envenomation.

scholarly journals Mast cells are responsible for the lack of anti-inflammatory effects of morphine in CBA mice

2004 ◽  
Vol 13 (5-6) ◽  
pp. 365-368 ◽  
Author(s):  
Elzbieta Stankiewicz ◽  
Ewa Wypasek ◽  
Barbara Plytycz
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Swiss Mice ◽  
Cba Mice ◽  
Peritoneal Mast Cells ◽  
Anti Inflammatory ◽  
Zymosan Peritonitis

BACKGROUND and aim: Morphine co-injection has anti-inflammatory effects on zymosan-induced peritonitis in several strains of mice except that of CBA. As peritoneal mast cells (pMCs) are much more numerous in CBA mice than in SWISS mice, the role of pMCs in morphine-modulated zymosan peritonitis is compared in CBA and SWISS males.Methods: pMCs were treatedin vitrowith morphine or C48/80 for comparison of histamine release.In vivoaccumulation of leukocytes and histamine in peritoneal exudate were recorded after intraperitoneal injection with morphine, zymosan, or zymosan plus morphine.Results and conclusion: Morphine induces histamine release by pMCs from CBA mice but not SWISS mice.In vivomorphine-induced peritonitis is stronger in CBA mice than SWISS mice. Corollary, morphine anti-inflammatory effects on zymosan peritonitis are reversed in CBA mice by its pro-inflammatory action through CBA pMCs.

Mast cell–lysosomal cationic protein interaction: effects of metabolic inhibitors and decay of activated cells on enhanced fluorescence of anilinonaphthalene sulfonate

1981 ◽  
Vol 59 (3) ◽  
pp. 202-207 ◽  
Author(s):  
Narendranath S. Ranadive ◽  
Deborah H. Ruben
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Metabolic Inhibitors ◽  
Early Event ◽  
High Concentration ◽  
Enhanced Fluorescence ◽  
Time Intervals ◽  
Cationic Protein ◽  
Peritoneal Mast Cells ◽  
Release Histamine

It has been shown earlier that the interactions of the isolated rat peritoneal mast cells with cationic protein from rabbit neutrophil lysosomes (band 2 protein) can be studied using anilinonaphthalene sulfonate (ANS) as a fluorescent probe. In the present communication, binding of ANS dye to the mast cells interacting with band 2 protein (B2) under various conditions has been reported. The inhibition of histamine release by metabolic inhibitors was found to have no effect on enhancement of ANS fluorescence. On the other hand, inhibition of histamine release at high concentration of Ca2+ (14.4 mM) was accompanied by the decrease in enhanced fluorescence. In the presence of 7.2 mM of Sr2+, the release of histamine was enhanced with small but significant increase in ANS fluorescence. The cells heated to 42 °C partially lost their capacity to release histamine without the loss of enhanced fluorescence. The mast cells interacting with B2 at 10 °C for various time intervals showed time-dependent loss in histamine releasing capacity with concomitant loss in enhanced fluorescence. These studies suggest that the enhancement of ANS fluorescence is associated with the early events of the cell membrane caused by interaction of B2 with the cells. The extracellular cations significantly influence this early event.

scholarly journals MAST CELL DEGRANULATION AND HISTAMINE RELEASE OBSERVED IN A NEW IN VITRO SYSTEM

1960 ◽  
Vol 112 (4) ◽  
pp. 571-580 ◽  
Author(s):  
David Lagunoff ◽  
Earl P. Benditt
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Polymyxin B ◽  
Millipore Filter ◽  
In Vitro System ◽  
Peritoneal Mast Cells ◽  
Vitro Analysis ◽  
In Vitro Analysis

A method is described by which mast cells harvested from the rat peritoneal cavity can be deposited on the surface of a Millipore filter without gross injury, permitting observation of morphological and chemical changes induced by a variety of agents. These isolated peritoneal mast cells respond to 48/80 and to polymyxin B but not to dextran or ovomucoid with degranulation and histamine release. Thus four agents which in vivo appear to have similar activities have been found by means of in vitro analysis to operate by at least two different mechanisms.

scholarly journals Further studies on the structural requirements for polypeptide-mediated histamine release from rat mast cells

1979 ◽  
Vol 181 (3) ◽  
pp. 623-632 ◽  
Author(s):  
B Jasani ◽  
G Kreil ◽  
B F Mackler ◽  
D R Stanworth
Keyword(s):  
Amino Acids ◽  
Mast Cells ◽  
Histamine Release ◽  
Immunoglobulin E ◽  
Polymyxin B ◽  
Carboxyl Group ◽  
Peritoneal Mast Cells ◽  
Normal Rat ◽  
Rat Mast Cells

Structure-activity studies have been performed on a series of naturally occurring and ‘tailor-made’ polypeptides, by measurement of ability to induce selective histamine release from normal rat peritoneal mast cells in vitro. Compounds investigated include corticotropin and melittin derivatives, mast-cell-degranulating peptide from bee venom, polymyxin B, bradykinin and various synthetic poly(amino acids) and short-chain peptides. It was confirmed that a cluster of four basic residues (lysine or arginine) was optimal for histamine release by corticotropin and melittin polypeptides, provided that the C-terminal carboxyl group was substituted (by, for instance, amidation). In contrast, the presence of a free C-terminal carboxyl group or nearby dicarboxylic acid residues led to a considerable diminution in histamine-releasing activity. Likewise, polypeptides comprised essentially of acidic amino acids were inactive. On the basis of these observations it has been possible to predict that synthetic peptides comprising a particular sequence within the Fc region of human immunoglobulin E, the immunoglobulin class particularly involved in mediation of allergic reactions of the immediate type, would possess potent histamine-releasing activity when similarly made to react with normal rat mast cells. The further study of such a structure should throw new light on the molecular basis of allergen-antibody triggering of mast cells.

scholarly journals Scutellariae radix. X. Inhibitory effects of various flavonoids on histamine release from rat peritoneal mast cells in vitro.

1984 ◽  
Vol 32 (12) ◽  
pp. 5051-5054 ◽  
Author(s):  
MICHINORI KUBO ◽  
HIDEAKI MATSUDA ◽  
YOSHIYUKI KIMURA ◽  
HIROMICHI OKUDA ◽  
SHIGERU ARICHI
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Inhibitory Effects ◽  
Scutellariae Radix ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

Tripterine: A Potential Anti-Allergic Compound

2020 ◽  
Vol 22 (1) ◽  
pp. 159-167
Author(s):  
Bao-Jun Zhu ◽  
Ze-Quan Qian ◽  
Hui-Run Yang ◽  
Ru-Xia Li
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Inflammatory Cells ◽  
Allergic Diseases ◽  
Inhibitory Effect ◽  
Inflammatory Factors ◽  
Peritoneal Mast Cells ◽  
Allergic Effect

Background: Tripterine (TRI), an active monomer in Tripterygium wilfordii, has significant pharmacological activities, such as anti-inflammatory, immunosuppressive and anti-tumor activities. TRI may be used to treat allergic diseases because of its characteristics of immunosuppression. Objective: This study aims to explore the anti-allergic effect of TRI. Methods: It was tested in vivo and in vitro in this study. Results : The results showed that TRI could significantly inhibit histamine release from rat peritoneal mast cells; the inhibitory effect of TRI on histamine release was stronger than that of other known histamine inhibitors such as disodium cromoglyceride. TRI also significantly inhibited systemic anaphylactic shock induced by compound 48/80 and skin allergy induced by IgE, and inhibited the expression of inflammatory factors secreted by Human Mast Cells (HMC-1) induced by Phorbol 12-Myristate 13- Acetate (PMA) and calcium carrier A23187. In the animal model of allergic rhinitis induced by Ovalbumin (OA), the scores of friction, histamine, IgE, inflammatory factors and inflammatory cells decreased after TRI was administered orally or nasally. Conclusions : TRI, as an active immunoregulatory factor, has great potential in the treatment of mast cell-mediated allergic diseases.

scholarly journals Vaginal Bacterial Flora Activates Rat Peritoneal Mast Cells

2002 ◽  
Vol 15 (3) ◽  
pp. 233-238 ◽  
Author(s):  
E. Brzezińska-Blaszczyk ◽  
M. Wasiela
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Ureaplasma Urealyticum ◽  
Bacterial Flora ◽  
Mycoplasma Hominis ◽  
Bacterial Strains ◽  
Histamine Secretion ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

Sixteen strains of physiological and pathological vaginal bacteria were tested for their ability to secrete histamine from rat peritoneal mast cells in vitro. We noticed that Mycoplasma hominis -induced histamine release was very high (up to 53.6%). The stimulation of rat mast cells with Staphylococccus cohnii, Staphylococcus coagulase(-) (two strains), Ureaplasma urealyticum, Peptostreptococcus spp., Bacteroides capillosus, Staphylococcus aureus and Streptococcus agalactiae resulted in lower, but significant histamine secretion (11.2%–17.5%). Other bacterial strains ( Staphylococcus epidermidids, Enterococcus faecalis, Escherichia coli, Actinomyces naeslundii (two strains) and Lactobacillus fermentum (two strains) caused very low (4.2 % – 8.8%) histamine release.

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