The use of lamellar body counts to predict fetal lung maturity in pregnancies complicated by diabetes mellitus

2002 ◽  
Vol 187 (4) ◽  
pp. 908-912 ◽  
Author(s):  
Michael E. DeRoche ◽  
Charles J. Ingardia ◽  
Paula J. Guerette ◽  
Alan H. Wu ◽  
Christine A. LaSala ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Lamellar Body ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Lung Maturity

scholarly journals Ultrasonographic evaluation of fetal lung histogram versus lamellar body count in the prediction of fetal lung maturity

2020 ◽  
Vol 10 (1) ◽  
pp. 6
Author(s):  
Waleed F Gharib ◽  
Tamer Y Mohamed ◽  
Mariam Lotfy ◽  
Mohamed S El-Tanab ◽  
Ahmed M Abbas ◽  
...  
Keyword(s):  
Lamellar Body ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Lung Maturity ◽  
Body Count

scholarly journals Lamellar Body Phospholipid Content of Amniotic Fluid: A Possible Index of Fetal Lung Maturity

1979 ◽  
Vol 16 (1-6) ◽  
pp. 191-196 ◽  
Author(s):  
Coral G. Duck-Chong
Keyword(s):  
Amniotic Fluid ◽  
Individual Variation ◽  
Lamellar Body ◽  
Alveolar Epithelium ◽  
Fetal Lung ◽  
Phospholipid Content ◽  
Secretory Cells ◽  
Lamellar Bodies ◽  
Fetal Lung Maturity ◽  
Lung Maturity

Lamellar bodies, produced by secretory cells in the alveolar epithelium, are the major source of surfactant phospholipid. As the fetal lung matures, the membranous content of the lamellar bodies is secreted into the alveolar spaces and passes into the amniotic fluid, from which it can be isolated in a morphologically recognisable form. A method is described for the rapid isolation of a lamellar body fraction from amniotic fluid using a small air-driven clinical ultracentrifuge. The lamellar body phospholipid content of amniotic fluid increases towards the end of gestation, but the time of onset and the rate of this increase show wide individual variation. Preliminary results suggest that the lamellar body phospholipid content of amniotic fluid may be a useful index of fetal lung maturity.

Lamellar body phospholipid content of amniotic fluid and L/S ratio compared in assessing fetal lung maturity.

1980 ◽  
Vol 26 (6) ◽  
pp. 766-769 ◽  
Author(s):  
C G Duck-Chong ◽  
J M Gupta ◽  
G N Storey ◽  
C R Houghton
Keyword(s):  
Amniotic Fluid ◽  
Lamellar Body ◽  
Fetal Lung ◽  
Hyaline Membrane Disease ◽  
Phospholipid Content ◽  
Human Amniotic Fluid ◽  
Respiratory Problems ◽  
Fetal Lung Maturity ◽  
Hyaline Membrane ◽  
Lung Maturity

Abstract A micro-method has been devised for isolating a lung-derived membranous fraction from human amniotic fluid. The phospholipid content of this fraction, known as lamellar body phospholipid, provides an indication of fetal lung maturity (Ann. Clin. Biochem 16: 191, 1979). This method has now been applied to 479 samples of amniotic fluid from 330 pregnancies. The lecithin/sphingomyelin ratio has also been determined for each of the samples by the routine method currently in use in the hospitals providing the samples. Hyaline membrane disease was associated with a low concentration of lamellar body phospholipid (< 35 mg/L) in all eight cases encountered in this study. In contrast, in 182 of the 185 cases where the lamellar body content of the amniotic fluid, collected within two days of delivery, exceeded 35 mg/L, the infants were free from serious respiratory problems. Data are presented which suggests that the lecithin/sphingomyelin ratio falsely indicated lung immaturity in many cases, amounting to 44% or more of all values indicating immaturity that were reported.

Lamellar body phospholipid content of amniotic fluid and L/S ratio compared in assessing fetal lung maturity.

1980 ◽  
Vol 26 (6) ◽  
pp. 766-769
Author(s):  
C G Duck-Chong ◽  
J M Gupta ◽  
G N Storey ◽  
C R Houghton
Keyword(s):  
Amniotic Fluid ◽  
Lamellar Body ◽  
Fetal Lung ◽  
Hyaline Membrane Disease ◽  
Phospholipid Content ◽  
Human Amniotic Fluid ◽  
Respiratory Problems ◽  
Fetal Lung Maturity ◽  
Hyaline Membrane ◽  
Lung Maturity

Abstract A micro-method has been devised for isolating a lung-derived membranous fraction from human amniotic fluid. The phospholipid content of this fraction, known as lamellar body phospholipid, provides an indication of fetal lung maturity (Ann. Clin. Biochem 16: 191, 1979). This method has now been applied to 479 samples of amniotic fluid from 330 pregnancies. The lecithin/sphingomyelin ratio has also been determined for each of the samples by the routine method currently in use in the hospitals providing the samples. Hyaline membrane disease was associated with a low concentration of lamellar body phospholipid (< 35 mg/L) in all eight cases encountered in this study. In contrast, in 182 of the 185 cases where the lamellar body content of the amniotic fluid, collected within two days of delivery, exceeded 35 mg/L, the infants were free from serious respiratory problems. Data are presented which suggests that the lecithin/sphingomyelin ratio falsely indicated lung immaturity in many cases, amounting to 44% or more of all values indicating immaturity that were reported.

543: Determining a cut-off for fetal lung maturity with lamellar body count testing

2007 ◽  
Vol 197 (6) ◽  
pp. S158
Author(s):  
Mary Beth Janicki ◽  
Lisa Dreiss ◽  
James Egan ◽  
Carolyn Zelop
Keyword(s):  
Lamellar Body ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Lung Maturity ◽  
Body Count

Lamellar Body Counts Compared With Traditional Phospholipid Analysis as an Assay for Evaluating Fetal Lung Maturity

2001 ◽  
Vol 97 (2) ◽  
pp. 305-309
Author(s):  
MARK G. NEERHOF ◽  
ELAINE I. HANEY ◽  
RICHARD K. SILVER ◽  
EDWARD R. ASHWOOD ◽  
IN-SIK LEE ◽  
...  
Keyword(s):  
Lamellar Body ◽  
Fetal Lung ◽  
Phospholipid Analysis ◽  
Fetal Lung Maturity ◽  
Lung Maturity

scholarly journals Comparative analysis of amniotic fluid lamellar body count and foam stability test as indices of fetal lung maturity

2010 ◽  
Vol 63 (11-12) ◽  
pp. 747-752 ◽  
Author(s):  
Jovana Visnjevac ◽  
Aleksandra Novakov-Mikic ◽  
Aleksandra Nikolic ◽  
Nemanja Visnjevac
Keyword(s):  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Amniotic Fluid ◽  
Distress Syndrome ◽  
Lamellar Body ◽  
Fetal Lung ◽  
Receiver Operating Characteristic Curves ◽  
Fetal Lung Maturity ◽  
Lung Maturity ◽  
Body Count

Introduction. Respiratory distress syndrome of the newborn caused by the fetal lung immaturity is a very serious clinical problem. Different tests of prenatal analysis of amniotic fluid, such as lamellar body count and Clements? test, are available for predicting the fetal lung maturity. Material and methods. A prospective clinical study was conducted on amniotic fluid samples from 2005 to 2006. The amniotic fluid samples were obtained at the gestational age of 30 to 42 weeks and collected by vaginal amniotomy, amniotomy during Caesarean section and 72 hours before the delivery by amniocentesis. A haematology analyzer (Nikon-Kohden?) was used to determine the lamellar body counts. Clements? test involved adding an equal volume of 96% ethanol to the multiple amniotic fluid volume (1:2, 1:4, 1:16, 1:32), followed by shaking and noting the presence of ring of bubbles. After the delivery, we compared the lamellar body count results and Clements? test and the outcome of pregnancies, primarily the development of respiratory distress syndrome. The most specific lamellar body cutoffs for maturity and immaturity were determined according to receiver operating characteristic curves. Results and Discussion. Out of 232 amniotic fluid samples which were tested, 112 samples were collected after vaginal amniotomy, 88 during the Caesarean delivery and 32 samples by amniocentesis. The overall incidence of respiratory distress syndrome was 14.6%. Receiver operating characteristic curves were used to identify cutoff points for the test. We found that both tests are good screening tests for predicting the fetal lung maturity with the area under the curve of 0.782 in Clements? test and 0.751 in the lamellar body count. Clements? cutoff 2 with sensitivity of 67.6% and specificity of 72.2%, proved best in the prediction of the fetal lung maturity. The lamellar body count cutoff of 42x10?/?l had the sensitivity of 82.4% and specificity of 64.6% in predicting the fetal lung maturity. Conclusion. Although both tests are good in predicting the fetal lung maturity, the lamellar body count has more advantages, because it is not only more objective, but also inexpensive, easy and fast to do, requires a small sample volume and is universally available.

W035 FETAL LUNG MATURITY AND LAMELLAR BODY COUNT

2012 ◽  
Vol 119 ◽  
pp. S716-S716
Author(s):  
T. Stimac ◽  
O. Petrovic ◽  
R. Krajina ◽  
H. Haller
Keyword(s):  
Lamellar Body ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Lung Maturity ◽  
Body Count
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