Expression of human telomerase reverse transcriptase, the catalytic subunit of telomerase, is associated with the development of persistent disease in complete hydatidiform moles

2001 ◽  
Vol 184 (7) ◽  
pp. 1441-1446 ◽  
Author(s):  
Charlie A. Amezcua ◽  
Afshin Bahador ◽  
Yathi M. Naidu ◽  
Juan C. Felix
Keyword(s):  
Reverse Transcriptase ◽  
Catalytic Subunit ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Persistent Disease ◽  
Human Telomerase ◽  
Hydatidiform Moles

scholarly journals Usefulness of Immunological Detection of the Human Telomerase Reverse Transcriptase

2005 ◽  
Vol 27 (5-6) ◽  
pp. 347-353
Author(s):  
Annalisa Volpi ◽  
Sara Bravaccini ◽  
Laura Medri ◽  
Serenella Cerasoli ◽  
Michele Gaudio ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Reverse Transcriptase ◽  
Precancerous Lesions ◽  
Pyramidal Cells ◽  
Catalytic Subunit ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Enzymatic Function ◽  
Somatic Tissues ◽  
Human Telomerase

Objective: Recent years have seen a considerable wealth of studies conducted on the potential usefulness of telomerase determination in diagnosis, prognosis and targeted cancer therapy. The frequently used Telomeric Repeat Amplification Protocol assay suffers from some drawbacks, the most important being the rate of false positives. In situ analysis using well characterised antibodies directed against the human telomerase reverse transcriptase (hTERT) would therefore appear to be important to morphologically identify the nature of telomerase positive cells. Methods: We performed immunostaining in a series of cultured cells and in normal, preneoplastic and tumour tissues from different organs using a monoclonal antibody directed against the catalytic subunit of telomerase. Results: Immunoreactivity was not observed in perennial cells of terminally differentiated cardiac and skeletal muscular tissues or in small pyramidal cells of the cerebral cortex. Conversely, it was found in other normal somatic tissues as well as in precancerous lesions and in all tumour histotypes. Conclusions: Immunohistochemistry with a well characterised hTERT-specific monoclonal antibody permitted the identification of hTERT immunopositive cells in normal somatic tissues. Whether hTERT protein detected by immunostaining with hTERT-specific Tel 3 36-10 antibody is actually the degraded form of the protein that retains hTERT antigenicity but not enzymatic function, or whether it represents the real, potentially functional catalytic subunit of the enzyme, immunohistochemistry would not seem to represent a useful tool to investigate the role of telomerase and the mechanisms involved in its regulation.

Gold Nanoprobe-Based Detection of Human Telomerase Reverse Transcriptase (hTERT) Gene Expression

2015 ◽  
Vol 14 (4) ◽  
pp. 485-490 ◽  
Author(s):  
Mohammad Pourhassan-Moghaddam ◽  
Nosratollah Zarghami ◽  
Afshin Mohsenifar ◽  
Mohammad Rahmati-Yamchi ◽  
Hadis Daraee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reverse Transcriptase ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Htert Gene ◽  
Human Telomerase ◽  
Gold Nanoprobe
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