Microsatellite analysis reveals a high incidence of maternal cell contamination in 46,XX products of conception consisting of villi or a combination of villi and membranous material

2001 ◽  
Vol 185 (1) ◽  
pp. 198-203 ◽  
Author(s):  
Kristine L. Jarrett ◽  
Ron C. Michaelis ◽  
Mary C. Phelan ◽  
Victoria A. Vincent ◽  
Robert G. Best
Keyword(s):  
Microsatellite Analysis ◽  
Maternal Cell ◽  
Products Of Conception ◽  
High Incidence ◽  
Membranous Material

scholarly journals High Incidence of Contaminating Maternal Cell Overgrowth in Human Placental Mesenchymal Stem/Stromal Cell Cultures: A Systematic Review

2014 ◽  
Vol 3 (11) ◽  
pp. 1305-1311 ◽  
Author(s):  
Celena F. Heazlewood ◽  
Helen Sherrell ◽  
Jennifer Ryan ◽  
Kerry Atkinson ◽  
Christine A. Wells ◽  
...  
Keyword(s):  
Systematic Review ◽  
Cell Cultures ◽  
Stromal Cell ◽  
Maternal Cell ◽  
High Incidence

Salvage of fetal karyotype information from SNP array data obtained from products of conception with maternal cell contamination

2017 ◽  
Vol 37 (8) ◽  
pp. 781-787 ◽  
Author(s):  
Karin Sasaki ◽  
Kosei Abe ◽  
Takahide Mori ◽  
Kazunori Hashimoto ◽  
Kazuhiko Nakabayashi
Keyword(s):  
Snp Array ◽  
Array Data ◽  
Maternal Cell ◽  
Products Of Conception ◽  
Fetal Karyotype

A high incidence of maternal cell contamination of amniotic fluid cell cultures

1983 ◽  
Vol 14 (2) ◽  
pp. 361-365 ◽  
Author(s):  
Peter A. Benn ◽  
Amy G. Schonhaut ◽  
Lillian Y. F. Hsu ◽  
Laurence E. Karp
Keyword(s):  
Amniotic Fluid ◽  
Cell Cultures ◽  
Amniotic Fluid Cell ◽  
Maternal Cell ◽  
High Incidence ◽  
Fluid Cell

scholarly journals Detection of cytogenomic abnormalities by OncoScan microarray assay for products of conception from formalin-fixed paraffin-embedded and fresh fetal tissues

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jiadi Wen ◽  
Brittany Grommisch ◽  
Autumn DiAdamo ◽  
Hongyan Chai ◽  
Sok Meng Evelyn Ng ◽  
...  
Keyword(s):  
False Negative ◽  
Microarray Assay ◽  
Maternal Cell ◽  
Fragmented Dna ◽  
Formalin Fixed Paraffin ◽  
Products Of Conception ◽  
Formalin Fixed Paraffin Embedded ◽  
Ffpe Tissues ◽  
Molecular Inversion Probes ◽  
Formalin Fixed

Abstract Background The OncoScan microarray assay (OMA) using highly multiplexed molecular inversion probes for single nucleotide polymorphism (SNP) loci enabled the detection of cytogenomic abnormalities of chromosomal imbalances and pathogenic copy number variants (pCNV). The small size of molecular inversion probes is optimal for SNP genotyping of fragmented DNA from fixed tissues. This retrospective study evaluated the clinical utility of OMA as a uniform platform to detect cytogenomic abnormalities for pregnancy loss from fresh and fixed tissues of products of conception (POC). Results Fresh specimens of POC were routinely subjected to cell culture and then analyzed by karyotyping. POC specimens with a normal karyotype (NK) or culture failure (CF) and from formalin-fixed paraffin-embedded (FFPE) tissues were subjected to DNA extraction for OMA. The abnormality detection rate (ADR) by OMA on 94 cases of POC-NK, 38 cases of POC-CF, and 35 cases of POC-FFPE tissues were 2% (2/94), 26% (10/38), and 57% (20/35), respectively. The detected cytogenomic abnormalities of aneuploidies, triploidies and pCNV accounted for 50%, 40% and 10% in POC-CF and 85%, 10% and 5% in POC-FFPE, respectively. False negative result from cultured maternal cells and maternal cell contamination were each detected in one case. OMA on two cases with unbalanced structural chromosome abnormalities further defined genomic imbalances and breakpoints. Conclusion OMA on POC-CF and POC-FFPE showed a high diagnostic yield of cytogenomic abnormalities. This approach circumvented the obstacles of CF from fresh specimens and fragmented DNA from fixed tissues and provided a reliable and effective platform for detecting cytogenomic abnormalities and monitoring true fetal result from maternal cell contamination.

Ultrastructure of Liver in Lactosyl Ceramidosis

1971 ◽  
Vol 29 ◽  
pp. 312-313
Author(s):  
Z. Hruban ◽  
J. R. Esterly ◽  
G. Dawson ◽  
A. O. Stein
Keyword(s):  
Connective Tissue ◽  
Liver Biopsy ◽  
Osmium Tetroxide ◽  
Close Contact ◽  
Multivesicular Bodies ◽  
Bile Canaliculi ◽  
Dense Bodies ◽  
Marginal Plates ◽  
Membranous Material ◽  
Perichromatin Granules

Samples of a surgical liver biopsy from a patient with lactosyl ceramidosis were fixed in paraformaldehyde and postfixed in osmium tetroxide. Hepatocytes (Figs. 1, 2) contained 0.4 to 2.1 μ inclusions (LCI) limited by a single membrane containing lucid matrix and short segments of curved, lamellated and circular membranous material (Fig. 3). Numerous LCI in large connective tissue cells were up to 11 μ in diameter (Fig. 2). Heterogeneous dense bodies (“lysosomes”) were few and irregularly distributed. Rough cisternae were dilated and contained smooth vesicles and surface invaginations. Close contact with mitochondria was rare. Stacks were small and rare. Vesicular rough reticulum and glycogen rosettes were abundant. Smooth vesicular reticulum was moderately abundant. Mitochondria were round with few cristae and rare matrical granules. Golgi complex was seen rarely (Fig. 1). Microbodies with marginal plates were usual. Multivesicular bodies were very rare. Neutral lipid was rare. Nucleoli were small and perichromatin granules were large. Small bile canaliculi had few microvilli (Fig. 1).

The observation of defects on (100) CdTe surfaces by chemical etching

1992 ◽  
Vol 50 (2) ◽  
pp. 1424-1425
Author(s):  
M.E. Lee
Keyword(s):  
Single Crystal ◽  
Grain Boundaries ◽  
Single Crystals ◽  
Chemical Etching ◽  
Crystalline Perfection ◽  
Crystalline Defects ◽  
High Incidence ◽  
Crystallographic Defects ◽  
Cdznte Substrates ◽  
Device Technology

The crystalline perfection of bulk CdTe substrates plays an important role in their use in infrared device technology. The application of chemical etchants to determine crystal polarity or the density and distribution of crystallographic defects in (100) CdTe is not well understood. The lack of data on (100) CdTe surfaces is a result of the apparent difficulty in growing (100) CdTe single crystal substrates which is caused by a high incidence of twinning. Many etchants have been reported to predict polarity on one or both (111) CdTe planes but are considered to be unsuitable as defect etchants. An etchant reported recently has been considered to be a true defect etchant for CdTe, MCT and CdZnTe substrates. This etchant has been reported to reveal crystalline defects such as dislocations, grain boundaries and inclusions in (110) and (111) CdTe. In this study the effect of this new etchant on (100) CdTe surfaces is investigated.The single crystals used in this study were (100) CdTe as-cut slices (1mm thickness) from Bridgman-grown ingots.

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