Development of the determination of ABO and Rh systems of blood group typing using acousto-optical technique

2021 ◽  
Author(s):  
Farah Mohammed Ali ◽  
Jamal A. Hasan ◽  
Eman Ghadhban Khalil
Keyword(s):  
Blood Group ◽  
Optical Technique ◽  
Blood Group Typing

scholarly journals Enhancement the Agglutination of Erythrocytes in Blood Typing Test by Acousto-Optic Technique

2020 ◽  
Vol 23 (4) ◽  
pp. 365-370
Author(s):  
Farah Mohammed Ali ◽  
Jamal A. Hasan ◽  
Eman Ghadhban Khalil
Keyword(s):  
Blood Group ◽  
Digital Image ◽  
High Efficiency ◽  
Digital Camera ◽  
Experimental Result ◽  
Optical Technique ◽  
Microscope Slide ◽  
Blood Group Typing ◽  
Different Color ◽  
Short Time

A proposed modern technique for determination the blood group typing by monitoring the agglutination of red blood cells using acousto-optical technique and digital camera. The method based on analysis the digital image of the agglutination process by MATLAB software. We present an overview of two acousto-optic sensing approaches; the first demonstrates the cuvette approach while the second is the microscope slide approach. The cuvette approach digital image analyzing depends on the green channel distribution of the original image and count the brighten pixels, while the microscope slide approach passes through series of algorithms started with grayscale filter and end with edge detection it counts the different color pixels. The experimental result shown that it is possible to enhance the determination of blood group typing by using acousto-optical technique in both cases of using isohemagglutinating sera as well as the crossmatch test in a short time and high efficiency compared with the traditional methods.

Biomimetic ABO Blood-Group Typing

2006 ◽  
Vol 45 (16) ◽  
pp. 2626-2629 ◽  
Author(s):  
Oliver Hayden ◽  
Karl-J. Mann ◽  
Stefan Krassnig ◽  
Franz L. Dickert
Keyword(s):  
Blood Group ◽  
Abo Blood Group ◽  
Blood Group Typing

Capture of red blood cells onto optical sensor for rapid ABO blood group typing and erythrocyte counting

2018 ◽  
Vol 262 ◽  
pp. 411-417 ◽  
Author(s):  
Xuemeng Li ◽  
Hanbo Feng ◽  
Yangyang Wang ◽  
Cuiping Zhou ◽  
Wei Jiang ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Group ◽  
Optical Sensor ◽  
Blood Cells ◽  
Abo Blood Group ◽  
Blood Group Typing

Enzymatic Determination of the Anomeric Structures of Two Blood-Group B Active Glycosphingolipids Recombined with Apolipoproteins

1978 ◽  
Vol 33 (1-2) ◽  
pp. 73-78 ◽  
Author(s):  
Peter Hanfland ◽  
Gerd Assmann ◽  
Heinz Egge
Keyword(s):  
Blood Group ◽  
Water Solubility ◽  
Erythrocyte Membranes ◽  
High Density Lipoproteins ◽  
Composition Analysis ◽  
Glycosidic Linkage ◽  
Anomeric Configuration ◽  
Enzymatic Determination ◽  
Group B

Abstract Anomeric configuration of oligosaccharides usually is established by specific glycosidases. For this purpose detergents achieving water solubility of primarily insoluble glycosphingolipids as substrates have been replaced by delipidated hum an serum high density lipoproteins. The new method, tested by several well characterized glycosphingolipids and glycosidases, finally was applied to the evaluation of anomeric structures of two blood-group B active glycosphingolipids [ceramide hexa-saccharide (B-I) and ceramide octasaccharide (B -II)] from hum an erythrocyte membranes. In both B-I and B-II, α-glycosidic linkage was dem onstrated for the term inal galactose and fucose residues. β-glycosidic linkage has been evaluated for backbone saccharides. Together with the results pre­ viously obtained by composition analysis, linkage analysis and sequence analysis the following complete structure can be established:B -I: Galα1 → 3Gal (2 ← 1αFuc)β1 → 4GlcNAcβ1 → 3Galβ1 → 4Glcβ1 → 1Cer;B-II: Galα1 → 3Gal (2 ← 1αFuc)β1 → 4GlcNAcβ1 → 3Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Glcβ1 → 1Cer.

scholarly journals Next-Generation Sequencing Technologies in Blood Group Typing

2019 ◽  
Vol 47 (1) ◽  
pp. 4-13 ◽  
Author(s):  
Daniel Fürst ◽  
Chrysanthi Tsamadou ◽  
Christine Neuchel ◽  
Hubert Schrezenmeier ◽  
Joannis Mytilineos ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Blood Group ◽  
Large Scale ◽  
Cost Effective ◽  
Molecular Testing ◽  
Blood Group Antigens ◽  
Next Generation ◽  
Sequencing Technologies ◽  
Blood Group Typing ◽  
Generation Sequencing

Sequencing of the human genome has led to the definition of the genes for most of the relevant blood group systems, and the polymorphisms responsible for most of the clinically relevant blood group antigens are characterized. Molecular blood group typing is used in situations where erythrocytes are not available or where serological testing was inconclusive or not possible due to the lack of antisera. Also, molecular testing may be more cost-effective in certain situations. Molecular typing approaches are mostly based on either PCR with specific primers, DNA hybridization, or DNA sequencing. Particularly the transition of sequencing techniques from Sanger-based sequencing to next-generation sequencing (NGS) technologies has led to exciting new possibilities in blood group genotyping. We describe briefly the currently available NGS platforms and their specifications, depict the genetic background of blood group polymorphisms, and discuss applications for NGS approaches in immunohematology. As an example, we delineate a protocol for large-scale donor blood group screening established and in use at our institution. Furthermore, we discuss technical challenges and limitations as well as the prospect for future developments, including long-read sequencing technologies.

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